data_26635 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, and 15N Chemical Shift Assignments for human K-Ras4B-GDP ; _BMRB_accession_number 26635 _BMRB_flat_file_name bmr26635.str _Entry_type original _Submission_date 2015-08-13 _Accession_date 2015-08-13 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Abraham Sherwin . . 2 Chavan Tanmay . . 3 Banerjee Avik . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 171 "15N chemical shifts" 171 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-05-25 update BMRB 'update entry citation' 2015-09-11 original author 'original release' stop_ _Original_release_date 2015-09-11 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Flexible-body motions of calmodulin and the farnesylated hypervariable region yield a high-affinity interaction enabling K-Ras4B membrane extraction ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 28623230 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jang Hyunbum . . 2 Banerjee Avik . . 3 Chavan Tanmay . . 4 Gaponenko Vadim . . 5 Nussinov Ruth . . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_name_full 'The Journal of biological chemistry' _Journal_volume 292 _Journal_issue 30 _Journal_ISSN 1083-351X _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 12544 _Page_last 12559 _Year 2017 _Details . loop_ _Keyword GDP K-Ras 'hypervariable region' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'human K-Ras4B-GDP' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'human K-Ras4B-GDP' $K-Ras4B stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_K-Ras4B _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common K-Ras4B _Molecular_mass . _Mol_thiol_state 'all free' _Details 'C-terminally His-tagged protein' ############################## # Polymer residue sequence # ############################## _Residue_count 188 _Mol_residue_sequence ; MTEYKLVVVGAGGVGKSALT IQLIQNHFVDEYDPTIEDSY RKQVVIDGETCLLDILDTAG QEEYSAMRDQYMRTGEGFLC VFAINNTKSFEDIHHYREQI KRVKDSEDVPMVLVGNKCDL PSRTVDTKQAQDLARSYGIP FIETSAKTRQGVDDAFYTLV REIRKHKEKMSKDGKKKKKK SKTKCVIM ; loop_ _Residue_seq_code _Residue_label 1 MET 2 THR 3 GLU 4 TYR 5 LYS 6 LEU 7 VAL 8 VAL 9 VAL 10 GLY 11 ALA 12 GLY 13 GLY 14 VAL 15 GLY 16 LYS 17 SER 18 ALA 19 LEU 20 THR 21 ILE 22 GLN 23 LEU 24 ILE 25 GLN 26 ASN 27 HIS 28 PHE 29 VAL 30 ASP 31 GLU 32 TYR 33 ASP 34 PRO 35 THR 36 ILE 37 GLU 38 ASP 39 SER 40 TYR 41 ARG 42 LYS 43 GLN 44 VAL 45 VAL 46 ILE 47 ASP 48 GLY 49 GLU 50 THR 51 CYS 52 LEU 53 LEU 54 ASP 55 ILE 56 LEU 57 ASP 58 THR 59 ALA 60 GLY 61 GLN 62 GLU 63 GLU 64 TYR 65 SER 66 ALA 67 MET 68 ARG 69 ASP 70 GLN 71 TYR 72 MET 73 ARG 74 THR 75 GLY 76 GLU 77 GLY 78 PHE 79 LEU 80 CYS 81 VAL 82 PHE 83 ALA 84 ILE 85 ASN 86 ASN 87 THR 88 LYS 89 SER 90 PHE 91 GLU 92 ASP 93 ILE 94 HIS 95 HIS 96 TYR 97 ARG 98 GLU 99 GLN 100 ILE 101 LYS 102 ARG 103 VAL 104 LYS 105 ASP 106 SER 107 GLU 108 ASP 109 VAL 110 PRO 111 MET 112 VAL 113 LEU 114 VAL 115 GLY 116 ASN 117 LYS 118 CYS 119 ASP 120 LEU 121 PRO 122 SER 123 ARG 124 THR 125 VAL 126 ASP 127 THR 128 LYS 129 GLN 130 ALA 131 GLN 132 ASP 133 LEU 134 ALA 135 ARG 136 SER 137 TYR 138 GLY 139 ILE 140 PRO 141 PHE 142 ILE 143 GLU 144 THR 145 SER 146 ALA 147 LYS 148 THR 149 ARG 150 GLN 151 GLY 152 VAL 153 ASP 154 ASP 155 ALA 156 PHE 157 TYR 158 THR 159 LEU 160 VAL 161 ARG 162 GLU 163 ILE 164 ARG 165 LYS 166 HIS 167 LYS 168 GLU 169 LYS 170 MET 171 SER 172 LYS 173 ASP 174 GLY 175 LYS 176 LYS 177 LYS 178 LYS 179 LYS 180 LYS 181 SER 182 LYS 183 THR 184 LYS 185 CYS 186 VAL 187 ILE 188 MET stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $K-Ras4B Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $K-Ras4B 'recombinant technology' . Escherichia coli . PET42a stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $K-Ras4B . mM '[U-13C; U-15N]' H2O 90 '% v/v' 'natural abundance' D2O 10 '% v/v' '[U-100% 2H]' TRIS 50 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' 'magenesium cloride' 10 mM 'natural abundance' beta-mercaptoethanol 5 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.105 . M pH 6.5 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0 na direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HN(CO)CA' '3D CBCA(CO)NH' '3D HNCA' '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'human K-Ras4B-GDP' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 THR H H 8.786 0.01 1 2 2 2 THR N N 124.13 0.01 1 3 3 3 GLU H H 8.388 0.01 1 4 3 3 GLU N N 127.30 0.01 1 5 4 4 TYR H H 8.716 0.01 1 6 4 4 TYR N N 121.83 0.01 1 7 5 5 LYS H H 9.126 0.01 1 8 5 5 LYS N N 124.57 0.01 1 9 6 6 LEU H H 9.506 0.01 1 10 6 6 LEU N N 126.87 0.01 1 11 8 8 VAL H H 8.941 0.01 1 12 8 8 VAL N N 129.09 0.01 1 13 9 9 VAL H H 9.116 0.01 1 14 9 9 VAL N N 120.54 0.01 1 15 10 10 GLY H H 7.079 0.01 1 16 10 10 GLY N N 107.47 0.01 1 17 11 11 ALA H H 9.140 0.01 1 18 11 11 ALA N N 123.77 0.01 1 19 12 12 GLY H H 8.581 0.01 1 20 12 12 GLY N N 106.33 0.01 1 21 13 13 GLY H H 10.501 0.01 1 22 13 13 GLY N N 115.17 0.01 1 23 15 15 GLY H H 8.544 0.01 1 24 15 15 GLY N N 109.60 0.01 1 25 17 17 SER H H 9.298 0.01 1 26 17 17 SER N N 120.46 0.01 1 27 18 18 ALA H H 9.460 0.01 1 28 18 18 ALA N N 125.42 0.01 1 29 19 19 LEU H H 8.991 0.01 1 30 19 19 LEU N N 120.40 0.01 1 31 20 20 THR H H 7.708 0.01 1 32 20 20 THR N N 116.87 0.01 1 33 21 21 ILE H H 8.863 0.01 1 34 21 21 ILE N N 120.74 0.01 1 35 22 22 GLN H H 7.813 0.01 1 36 22 22 GLN N N 120.93 0.01 1 37 23 23 LEU H H 7.609 0.01 1 38 23 23 LEU N N 120.74 0.01 1 39 24 24 ILE H H 8.005 0.01 1 40 24 24 ILE N N 114.14 0.01 1 41 25 25 GLN H H 8.954 0.01 1 42 25 25 GLN N N 116.02 0.01 1 43 26 26 ASN H H 7.918 0.01 1 44 26 26 ASN N N 116.15 0.01 1 45 28 28 PHE H H 8.433 0.01 1 46 28 28 PHE N N 122.56 0.01 1 47 29 29 VAL H H 7.646 0.01 1 48 29 29 VAL N N 126.06 0.01 1 49 30 30 ASP H H 7.744 0.01 1 50 30 30 ASP N N 122.34 0.01 1 51 31 31 GLU H H 7.635 0.01 1 52 31 31 GLU N N 119.79 0.01 1 53 32 32 TYR H H 8.762 0.01 1 54 32 32 TYR N N 125.66 0.01 1 55 33 33 ASP H H 7.818 0.01 1 56 33 33 ASP N N 128.89 0.01 1 57 35 35 THR H H 8.951 0.01 1 58 35 35 THR N N 109.93 0.01 1 59 36 36 ILE H H 6.776 0.01 1 60 36 36 ILE N N 120.90 0.01 1 61 37 37 GLU H H 8.383 0.01 1 62 37 37 GLU N N 132.24 0.01 1 63 38 38 ASP H H 8.114 0.01 1 64 38 38 ASP N N 124.48 0.01 1 65 39 39 SER H H 8.363 0.01 1 66 39 39 SER N N 114.04 0.01 1 67 40 40 TYR H H 9.065 0.01 1 68 40 40 TYR N N 121.28 0.01 1 69 41 41 ARG H H 8.348 0.01 1 70 41 41 ARG N N 119.75 0.01 1 71 42 42 LYS H H 8.516 0.01 1 72 42 42 LYS N N 122.27 0.01 1 73 43 43 GLN H H 8.800 0.01 1 74 43 43 GLN N N 128.92 0.01 1 75 44 44 VAL H H 9.056 0.01 1 76 44 44 VAL N N 121.20 0.01 1 77 46 46 ILE H H 8.259 0.01 1 78 46 46 ILE N N 126.36 0.01 1 79 47 47 ASP H H 9.498 0.01 1 80 47 47 ASP N N 130.34 0.01 1 81 48 48 GLY H H 8.231 0.01 1 82 48 48 GLY N N 103.52 0.01 1 83 49 49 GLU H H 7.656 0.01 1 84 49 49 GLU N N 123.08 0.01 1 85 50 50 THR H H 8.920 0.01 1 86 50 50 THR N N 126.12 0.01 1 87 51 51 CYS H H 9.338 0.01 1 88 51 51 CYS N N 125.48 0.01 1 89 52 52 LEU H H 8.706 0.01 1 90 52 52 LEU N N 122.94 0.01 1 91 53 53 LEU H H 9.031 0.01 1 92 53 53 LEU N N 124.20 0.01 1 93 54 54 ASP H H 8.630 0.01 1 94 54 54 ASP N N 125.66 0.01 1 95 55 55 ILE H H 9.144 0.01 1 96 55 55 ILE N N 123.77 0.01 1 97 56 56 LEU H H 8.619 0.01 1 98 56 56 LEU N N 127.51 0.01 1 99 57 57 ASP H H 8.432 0.01 1 100 57 57 ASP N N 129.29 0.01 1 101 58 58 THR H H 6.703 0.01 1 102 58 58 THR N N 110.03 0.01 1 103 59 59 ALA H H 9.046 0.01 1 104 59 59 ALA N N 121.86 0.01 1 105 60 60 GLY H H 8.230 0.01 1 106 60 60 GLY N N 108.01 0.01 1 107 61 61 GLN H H 8.559 0.01 1 108 61 61 GLN N N 119.12 0.01 1 109 62 62 GLU H H 8.681 0.01 1 110 62 62 GLU N N 120.61 0.01 1 111 63 63 GLU H H 8.255 0.01 1 112 63 63 GLU N N 120.16 0.01 1 113 64 64 TYR H H 8.244 0.01 1 114 64 64 TYR N N 121.13 0.01 1 115 65 65 SER H H 7.833 0.01 1 116 65 65 SER N N 120.39 0.01 1 117 66 66 ALA H H 8.126 0.01 1 118 66 66 ALA N N 121.32 0.01 1 119 67 67 MET H H 8.214 0.01 1 120 67 67 MET N N 117.79 0.01 1 121 68 68 ARG H H 7.813 0.01 1 122 68 68 ARG N N 120.93 0.01 1 123 69 69 ASP H H 8.074 0.01 1 124 69 69 ASP N N 118.58 0.01 1 125 70 70 GLN H H 7.785 0.01 1 126 70 70 GLN N N 117.49 0.01 1 127 71 71 TYR H H 8.217 0.01 1 128 71 71 TYR N N 119.39 0.01 1 129 72 72 MET H H 8.433 0.01 1 130 72 72 MET N N 118.73 0.01 1 131 73 73 ARG H H 7.922 0.01 1 132 73 73 ARG N N 115.71 0.01 1 133 74 74 THR H H 7.886 0.01 1 134 74 74 THR N N 108.05 0.01 1 135 75 75 GLY H H 7.952 0.01 1 136 75 75 GLY N N 111.25 0.01 1 137 76 76 GLU H H 8.842 0.01 1 138 76 76 GLU N N 121.94 0.01 1 139 77 77 GLY H H 7.135 0.01 1 140 77 77 GLY N N 100.83 0.01 1 141 78 78 PHE H H 8.126 0.01 1 142 78 78 PHE N N 121.31 0.01 1 143 79 79 LEU H H 9.175 0.01 1 144 79 79 LEU N N 126.93 0.01 1 145 80 80 CYS H H 8.679 0.01 1 146 80 80 CYS N N 124.71 0.01 1 147 81 81 VAL H H 9.010 0.01 1 148 81 81 VAL N N 126.42 0.01 1 149 82 82 PHE H H 9.253 0.01 1 150 82 82 PHE N N 123.64 0.01 1 151 83 83 ALA H H 8.688 0.01 1 152 83 83 ALA N N 121.38 0.01 1 153 84 84 ILE H H 8.452 0.01 1 154 84 84 ILE N N 113.56 0.01 1 155 85 85 ASN H H 7.839 0.01 1 156 85 85 ASN N N 116.95 0.01 1 157 86 86 ASN H H 7.833 0.01 1 158 86 86 ASN N N 119.23 0.01 1 159 87 87 THR H H 8.571 0.01 1 160 87 87 THR N N 121.80 0.01 1 161 88 88 LYS H H 8.397 0.01 1 162 88 88 LYS N N 124.10 0.01 1 163 89 89 SER H H 8.028 0.01 1 164 89 89 SER N N 114.36 0.01 1 165 90 90 PHE H H 7.369 0.01 1 166 90 90 PHE N N 124.67 0.01 1 167 91 91 GLU H H 8.401 0.01 1 168 91 91 GLU N N 121.86 0.01 1 169 92 92 ASP H H 8.421 0.01 1 170 92 92 ASP N N 116.88 0.01 1 171 93 93 ILE H H 7.551 0.01 1 172 93 93 ILE N N 120.71 0.01 1 173 94 94 HIS H H 7.651 0.01 1 174 94 94 HIS N N 116.95 0.01 1 175 95 95 HIS H H 7.196 0.01 1 176 95 95 HIS N N 117.18 0.01 1 177 96 96 TYR H H 7.670 0.01 1 178 96 96 TYR N N 118.89 0.01 1 179 97 97 ARG H H 8.434 0.01 1 180 97 97 ARG N N 118.73 0.01 1 181 98 98 GLU H H 7.884 0.01 1 182 98 98 GLU N N 117.63 0.01 1 183 99 99 GLN H H 7.819 0.01 1 184 99 99 GLN N N 120.10 0.01 1 185 100 100 ILE H H 7.635 0.01 1 186 100 100 ILE N N 119.78 0.01 1 187 101 101 LYS H H 7.784 0.01 1 188 101 101 LYS N N 117.50 0.01 1 189 102 102 ARG H H 7.693 0.01 1 190 102 102 ARG N N 117.69 0.01 1 191 103 103 VAL H H 7.987 0.01 1 192 103 103 VAL N N 118.05 0.01 1 193 104 104 LYS H H 7.947 0.01 1 194 104 104 LYS N N 117.23 0.01 1 195 105 105 ASP H H 7.919 0.01 1 196 105 105 ASP N N 120.87 0.01 1 197 106 106 SER H H 7.549 0.01 1 198 106 106 SER N N 109.10 0.01 1 199 107 107 GLU H H 8.350 0.01 1 200 107 107 GLU N N 121.68 0.01 1 201 108 108 ASP H H 8.321 0.01 1 202 108 108 ASP N N 121.35 0.01 1 203 109 109 VAL H H 7.525 0.01 1 204 109 109 VAL N N 121.48 0.01 1 205 111 111 MET H H 8.123 0.01 1 206 111 111 MET N N 122.67 0.01 1 207 112 112 VAL H H 7.979 0.01 1 208 112 112 VAL N N 118.06 0.01 1 209 113 113 LEU H H 8.903 0.01 1 210 113 113 LEU N N 128.87 0.01 1 211 114 114 VAL H H 9.157 0.01 1 212 114 114 VAL N N 128.17 0.01 1 213 115 115 GLY H H 8.158 0.01 1 214 115 115 GLY N N 113.46 0.01 1 215 116 116 ASN H H 8.758 0.01 1 216 116 116 ASN N N 121.47 0.01 1 217 118 118 CYS H H 8.634 0.01 1 218 118 118 CYS N N 114.09 0.01 1 219 119 119 ASP H H 8.548 0.01 1 220 119 119 ASP N N 116.88 0.01 1 221 120 120 LEU H H 7.759 0.01 1 222 120 120 LEU N N 121.57 0.01 1 223 122 122 SER H H 7.222 0.01 1 224 122 122 SER N N 113.12 0.01 1 225 124 124 THR H H 9.012 0.01 1 226 124 124 THR N N 114.21 0.01 1 227 125 125 VAL H H 7.508 0.01 1 228 125 125 VAL N N 124.21 0.01 1 229 126 126 ASP H H 8.557 0.01 1 230 126 126 ASP N N 128.45 0.01 1 231 128 128 LYS H H 8.368 0.01 1 232 128 128 LYS N N 121.06 0.01 1 233 129 129 GLN H H 7.295 0.01 1 234 129 129 GLN N N 117.49 0.01 1 235 130 130 ALA H H 7.045 0.01 1 236 130 130 ALA N N 122.45 0.01 1 237 131 131 GLN H H 8.383 0.01 1 238 131 131 GLN N N 117.82 0.01 1 239 132 132 ASP H H 8.478 0.01 1 240 132 132 ASP N N 120.40 0.01 1 241 133 133 LEU H H 7.477 0.01 1 242 133 133 LEU N N 123.60 0.01 1 243 134 134 ALA H H 8.206 0.01 1 244 134 134 ALA N N 121.60 0.01 1 245 135 135 ARG H H 8.420 0.01 1 246 135 135 ARG N N 118.25 0.01 1 247 136 136 SER H H 7.885 0.01 1 248 136 136 SER N N 117.64 0.01 1 249 137 137 TYR H H 7.529 0.01 1 250 137 137 TYR N N 119.78 0.01 1 251 138 138 GLY H H 8.237 0.01 1 252 138 138 GLY N N 110.91 0.01 1 253 139 139 ILE H H 7.985 0.01 1 254 139 139 ILE N N 112.88 0.01 1 255 141 141 PHE H H 8.180 0.01 1 256 141 141 PHE N N 119.91 0.01 1 257 142 142 ILE H H 8.346 0.01 1 258 142 142 ILE N N 129.97 0.01 1 259 143 143 GLU H H 7.747 0.01 1 260 143 143 GLU N N 125.10 0.01 1 261 144 144 THR H H 8.719 0.01 1 262 144 144 THR N N 112.06 0.01 1 263 145 145 SER H H 8.740 0.01 1 264 145 145 SER N N 112.45 0.01 1 265 146 146 ALA H H 9.096 0.01 1 266 146 146 ALA N N 132.40 0.01 1 267 147 147 LYS H H 6.945 0.01 1 268 147 147 LYS N N 116.17 0.01 1 269 148 148 THR H H 7.643 0.01 1 270 148 148 THR N N 106.41 0.01 1 271 149 149 ARG H H 7.761 0.01 1 272 149 149 ARG N N 119.46 0.01 1 273 150 150 GLN H H 7.750 0.01 1 274 150 150 GLN N N 128.28 0.01 1 275 151 151 GLY H H 8.878 0.01 1 276 151 151 GLY N N 115.30 0.01 1 277 152 152 VAL H H 6.968 0.01 1 278 152 152 VAL N N 120.57 0.01 1 279 153 153 ASP H H 7.882 0.01 1 280 153 153 ASP N N 117.14 0.01 1 281 154 154 ASP H H 7.997 0.01 1 282 154 154 ASP N N 116.30 0.01 1 283 155 155 ALA H H 8.573 0.01 1 284 155 155 ALA N N 124.85 0.01 1 285 156 156 PHE H H 7.175 0.01 1 286 156 156 PHE N N 113.03 0.01 1 287 157 157 TYR H H 9.557 0.01 1 288 157 157 TYR N N 119.72 0.01 1 289 158 158 THR H H 8.557 0.01 1 290 158 158 THR N N 116.50 0.01 1 291 159 159 LEU H H 7.243 0.01 1 292 159 159 LEU N N 121.95 0.01 1 293 160 160 VAL H H 7.592 0.01 1 294 160 160 VAL N N 118.76 0.01 1 295 161 161 ARG H H 8.203 0.01 1 296 161 161 ARG N N 118.78 0.01 1 297 162 162 GLU H H 7.979 0.01 1 298 162 162 GLU N N 118.04 0.01 1 299 163 163 ILE H H 8.375 0.01 1 300 163 163 ILE N N 123.67 0.01 1 301 164 164 ARG H H 8.134 0.01 1 302 164 164 ARG N N 118.43 0.01 1 303 166 166 HIS H H 7.985 0.01 1 304 166 166 HIS N N 125.47 0.01 1 305 167 167 LYS H H 8.080 0.01 1 306 167 167 LYS N N 122.28 0.01 1 307 169 169 LYS H H 8.184 0.01 1 308 169 169 LYS N N 115.39 0.01 1 309 170 170 MET H H 8.520 0.01 1 310 170 170 MET N N 122.28 0.01 1 311 171 171 SER H H 8.383 0.01 1 312 171 171 SER N N 117.82 0.01 1 313 173 173 ASP H H 8.350 0.01 1 314 173 173 ASP N N 121.68 0.01 1 315 174 174 GLY H H 8.174 0.01 1 316 174 174 GLY N N 108.81 0.01 1 317 175 175 LYS H H 8.045 0.01 1 318 175 175 LYS N N 120.36 0.01 1 319 176 176 LYS H H 8.314 0.01 1 320 176 176 LYS N N 123.29 0.01 1 321 177 177 LYS H H 8.230 0.01 1 322 177 177 LYS N N 122.65 0.01 1 323 178 178 LYS H H 8.375 0.01 1 324 178 178 LYS N N 123.68 0.01 1 325 179 179 LYS H H 8.000 0.01 1 326 179 179 LYS N N 123.28 0.01 1 327 180 180 LYS H H 8.450 0.01 1 328 180 180 LYS N N 123.74 0.01 1 329 181 181 SER H H 8.422 0.01 1 330 181 181 SER N N 118.26 0.01 1 331 182 182 LYS H H 8.398 0.01 1 332 182 182 LYS N N 124.10 0.01 1 333 183 183 THR H H 8.159 0.01 1 334 183 183 THR N N 116.07 0.01 1 335 185 185 CYS H H 8.255 0.01 1 336 185 185 CYS N N 120.16 0.01 1 337 186 186 VAL H H 8.246 0.01 1 338 186 186 VAL N N 123.62 0.01 1 339 187 187 ILE H H 8.266 0.01 1 340 187 187 ILE N N 125.83 0.01 1 341 188 188 MET H H 8.009 0.01 1 342 188 188 MET N N 128.05 0.01 1 stop_ save_