data_26792 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone assignment of p23 GOLD domain ; _BMRB_accession_number 26792 _BMRB_flat_file_name bmr26792.str _Entry_type original _Submission_date 2016-05-10 _Accession_date 2016-05-10 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Yamaguchi Yoshiki . . 2 Nagae Masamichi . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 81 "13C chemical shifts" 162 "15N chemical shifts" 81 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2016-10-14 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 26793 'Backbone assignment of p24 GOLD domain' stop_ _Original_release_date 2016-10-14 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; 3D structure and interaction of p24beta and p24delta Golgi dynamics domains: implications for p24 complex formation and cargo transport ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 27569046 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Nagae Masamichi . . 2 Hirata Tetsuya . . 3 Morita-Matsumoto Kana . . 4 Theiler Romina . . 5 Fujita Morihisa . . 6 Kinoshita Taroh . . 7 Yamaguchi Yoshiki . . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 428 _Journal_issue 20 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 4087 _Page_last 4099 _Year 2016 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name monomer _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'GOLD domain' $p23 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_p23 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common p23 _Molecular_mass . _Mol_thiol_state 'all disulfide bound' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 103 _Mol_residue_sequence ; GSISFHLPVNSRKCLREEIH KDLLVTGAYEITDQSGGAGG LRTHLKITDSAGHILYAKED ATKGKFAFTTEDYDMFEVCF ESKGTGRIPDQLVILDMKHG VEA ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 30 GLY 2 31 SER 3 32 ILE 4 33 SER 5 34 PHE 6 35 HIS 7 36 LEU 8 37 PRO 9 38 VAL 10 39 ASN 11 40 SER 12 41 ARG 13 42 LYS 14 43 CYS 15 44 LEU 16 45 ARG 17 46 GLU 18 47 GLU 19 48 ILE 20 49 HIS 21 50 LYS 22 51 ASP 23 52 LEU 24 53 LEU 25 54 VAL 26 55 THR 27 56 GLY 28 57 ALA 29 58 TYR 30 59 GLU 31 60 ILE 32 61 THR 33 62 ASP 34 63 GLN 35 64 SER 36 65 GLY 37 66 GLY 38 67 ALA 39 68 GLY 40 69 GLY 41 70 LEU 42 71 ARG 43 72 THR 44 73 HIS 45 74 LEU 46 75 LYS 47 76 ILE 48 77 THR 49 78 ASP 50 79 SER 51 80 ALA 52 81 GLY 53 82 HIS 54 83 ILE 55 84 LEU 56 85 TYR 57 86 ALA 58 87 LYS 59 88 GLU 60 89 ASP 61 90 ALA 62 91 THR 63 92 LYS 64 93 GLY 65 94 LYS 66 95 PHE 67 96 ALA 68 97 PHE 69 98 THR 70 99 THR 71 100 GLU 72 101 ASP 73 102 TYR 74 103 ASP 75 104 MET 76 105 PHE 77 106 GLU 78 107 VAL 79 108 CYS 80 109 PHE 81 110 GLU 82 111 SER 83 112 LYS 84 113 GLY 85 114 THR 86 115 GLY 87 116 ARG 88 117 ILE 89 118 PRO 90 119 ASP 91 120 GLN 92 121 LEU 93 122 VAL 94 123 ILE 95 124 LEU 96 125 ASP 97 126 MET 98 127 LYS 99 128 HIS 100 129 GLY 101 130 VAL 102 131 GLU 103 132 ALA stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $p23 'Norway rat' 10116 Eukaryota Metazoa Rattus norvegicus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $p23 'recombinant technology' . Escherichia coli . pCold stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $p23 0.5 mM '[U-99% 13C; U-99% 15N]' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' 'sodium phosphate' 20 mM 'natural abundance' NaCl 50 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 150 . mM pH 6.5 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCA' '3D HN(CO)CA' '3D HNCACB' '3D CBCA(CO)NH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'GOLD domain' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 31 2 SER H H 8.52 0.02 1 2 31 2 SER CA C 53.0 0.2 1 3 31 2 SER CB C 60.3 0.2 1 4 31 2 SER N N 116.7 0.2 1 5 32 3 ILE H H 8.96 0.02 1 6 32 3 ILE CA C 55.4 0.2 1 7 32 3 ILE CB C 37.6 0.2 1 8 32 3 ILE N N 124.0 0.2 1 9 33 4 SER H H 8.03 0.02 1 10 33 4 SER CA C 50.9 0.2 1 11 33 4 SER CB C 61.8 0.2 1 12 33 4 SER N N 117.9 0.2 1 13 34 5 PHE H H 8.37 0.02 1 14 34 5 PHE CA C 51.1 0.2 1 15 34 5 PHE CB C 35.9 0.2 1 16 34 5 PHE N N 122.6 0.2 1 17 35 6 HIS H H 8.78 0.02 1 18 35 6 HIS CA C 51.0 0.2 1 19 35 6 HIS CB C 26.7 0.2 1 20 35 6 HIS N N 117.8 0.2 1 21 36 7 LEU H H 9.19 0.02 1 22 36 7 LEU CB C 41.7 0.2 1 23 36 7 LEU N N 124.7 0.2 1 24 37 8 PRO CA C 57.2 0.2 1 25 37 8 PRO CB C 28.0 0.2 1 26 38 9 VAL H H 8.49 0.02 1 27 38 9 VAL CA C 57.9 0.2 1 28 38 9 VAL CB C 26.8 0.2 1 29 38 9 VAL N N 123.2 0.2 1 30 39 10 ASN H H 6.92 0.02 1 31 39 10 ASN CA C 50.0 0.2 1 32 39 10 ASN CB C 32.4 0.2 1 33 39 10 ASN N N 119.2 0.2 1 34 40 11 SER H H 7.57 0.02 1 35 40 11 SER CA C 52.7 0.2 1 36 40 11 SER CB C 62.0 0.2 1 37 40 11 SER N N 112.6 0.2 1 38 41 12 ARG H H 8.07 0.02 1 39 41 12 ARG CA C 49.4 0.2 1 40 41 12 ARG CB C 29.8 0.2 1 41 41 12 ARG N N 119.5 0.2 1 42 42 13 LYS H H 8.94 0.02 1 43 42 13 LYS CA C 50.6 0.2 1 44 42 13 LYS CB C 31.8 0.2 1 45 42 13 LYS N N 126.8 0.2 1 46 43 14 CYS H H 8.57 0.02 1 47 43 14 CYS CA C 50.1 0.2 1 48 43 14 CYS CB C 44.8 0.2 1 49 43 14 CYS N N 122.1 0.2 1 50 44 15 LEU H H 9.06 0.02 1 51 44 15 LEU CA C 49.0 0.2 1 52 44 15 LEU CB C 39.5 0.2 1 53 44 15 LEU N N 124.7 0.2 1 54 45 16 ARG H H 8.39 0.02 1 55 45 16 ARG CA C 49.7 0.2 1 56 45 16 ARG CB C 28.6 0.2 1 57 45 16 ARG N N 122.8 0.2 1 58 46 17 GLU H H 8.97 0.02 1 59 46 17 GLU CA C 56.1 0.2 1 60 46 17 GLU CB C 28.4 0.2 1 61 46 17 GLU N N 126.2 0.2 1 62 54 25 VAL CA C 56.2 0.2 1 63 54 25 VAL CB C 27.5 0.2 1 64 55 26 THR H H 8.15 0.02 1 65 55 26 THR CA C 54.1 0.2 1 66 55 26 THR CB C 67.3 0.2 1 67 55 26 THR N N 115.4 0.2 1 68 56 27 GLY H H 7.77 0.02 1 69 56 27 GLY CA C 39.7 0.2 1 70 56 27 GLY N N 109.2 0.2 1 71 57 28 ALA H H 8.43 0.02 1 72 57 28 ALA CA C 46.0 0.2 1 73 57 28 ALA CB C 17.6 0.2 1 74 57 28 ALA N N 126.3 0.2 1 75 58 29 TYR H H 8.29 0.02 1 76 58 29 TYR CA C 50.1 0.2 1 77 58 29 TYR CB C 38.6 0.2 1 78 58 29 TYR N N 117.2 0.2 1 79 59 30 GLU H H 8.62 0.02 1 80 59 30 GLU CA C 52.0 0.2 1 81 59 30 GLU CB C 28.5 0.2 1 82 59 30 GLU N N 120.3 0.2 1 83 60 31 ILE H H 8.70 0.02 1 84 60 31 ILE CA C 55.7 0.2 1 85 60 31 ILE CB C 34.6 0.2 1 86 60 31 ILE N N 127.0 0.2 1 87 61 32 THR H H 9.31 0.02 1 88 61 32 THR CA C 57.1 0.2 1 89 61 32 THR CB C 66.1 0.2 1 90 61 32 THR N N 123.9 0.2 1 91 62 33 ASP H H 8.61 0.02 1 92 62 33 ASP CA C 48.0 0.2 1 93 62 33 ASP CB C 37.7 0.2 1 94 62 33 ASP N N 124.9 0.2 1 95 63 34 GLN H H 8.79 0.02 1 96 63 34 GLN CA C 51.6 0.2 1 97 63 34 GLN CB C 24.9 0.2 1 98 63 34 GLN N N 124.2 0.2 1 99 64 35 SER H H 8.57 0.02 1 100 64 35 SER CA C 54.8 0.2 1 101 64 35 SER CB C 60.8 0.2 1 102 64 35 SER N N 116.5 0.2 1 103 65 36 GLY H H 8.01 0.02 1 104 65 36 GLY CA C 41.5 0.2 1 105 65 36 GLY N N 111.5 0.2 1 106 66 37 GLY H H 8.43 0.02 1 107 66 37 GLY CA C 40.7 0.2 1 108 66 37 GLY N N 109.1 0.2 1 109 67 38 ALA H H 7.60 0.02 1 110 67 38 ALA CA C 47.9 0.2 1 111 67 38 ALA CB C 15.0 0.2 1 112 67 38 ALA N N 123.9 0.2 1 113 68 39 GLY H H 8.33 0.02 1 114 68 39 GLY CA C 41.4 0.2 1 115 68 39 GLY N N 106.2 0.2 1 116 69 40 GLY H H 8.11 0.02 1 117 69 40 GLY CA C 41.3 0.2 1 118 69 40 GLY N N 107.9 0.2 1 119 70 41 LEU H H 7.25 0.02 1 120 70 41 LEU CA C 50.6 0.2 1 121 70 41 LEU CB C 39.1 0.2 1 122 70 41 LEU N N 120.4 0.2 1 123 71 42 ARG H H 8.43 0.02 1 124 71 42 ARG CA C 50.5 0.2 1 125 71 42 ARG CB C 30.2 0.2 1 126 71 42 ARG N N 124.5 0.2 1 127 72 43 THR H H 8.90 0.02 1 128 72 43 THR CA C 57.2 0.2 1 129 72 43 THR CB C 65.6 0.2 1 130 72 43 THR N N 120.3 0.2 1 131 73 44 HIS H H 9.03 0.02 1 132 73 44 HIS CA C 49.0 0.2 1 133 73 44 HIS CB C 29.2 0.2 1 134 73 44 HIS N N 124.7 0.2 1 135 74 45 LEU H H 8.41 0.02 1 136 74 45 LEU CA C 48.4 0.2 1 137 74 45 LEU CB C 41.2 0.2 1 138 74 45 LEU N N 126.5 0.2 1 139 75 46 LYS H H 8.81 0.02 1 140 75 46 LYS CA C 51.3 0.2 1 141 75 46 LYS CB C 32.5 0.2 1 142 75 46 LYS N N 130.5 0.2 1 143 76 47 ILE H H 9.02 0.02 1 144 76 47 ILE CA C 55.4 0.2 1 145 76 47 ILE CB C 34.2 0.2 1 146 76 47 ILE N N 127.6 0.2 1 147 77 48 THR H H 8.85 0.02 1 148 77 48 THR CA C 54.0 0.2 1 149 77 48 THR CB C 67.9 0.2 1 150 77 48 THR N N 118.3 0.2 1 151 78 49 ASP H H 7.59 0.02 1 152 78 49 ASP CA C 47.4 0.2 1 153 78 49 ASP CB C 36.8 0.2 1 154 78 49 ASP N N 118.3 0.2 1 155 79 50 SER CA C 56.6 0.2 1 156 79 50 SER CB C 58.1 0.2 1 157 80 51 ALA H H 7.57 0.02 1 158 80 51 ALA CA C 46.7 0.2 1 159 80 51 ALA CB C 14.6 0.2 1 160 80 51 ALA N N 124.0 0.2 1 161 81 52 GLY H H 7.70 0.02 1 162 81 52 GLY CA C 40.2 0.2 1 163 81 52 GLY N N 106.5 0.2 1 164 82 53 HIS H H 8.52 0.02 1 165 82 53 HIS CA C 51.3 0.2 1 166 82 53 HIS CB C 23.2 0.2 1 167 82 53 HIS N N 121.1 0.2 1 168 83 54 ILE H H 8.47 0.02 1 169 83 54 ILE CA C 56.9 0.2 1 170 83 54 ILE CB C 32.7 0.2 1 171 83 54 ILE N N 118.2 0.2 1 172 84 55 LEU H H 8.30 0.02 1 173 84 55 LEU CA C 50.2 0.2 1 174 84 55 LEU CB C 37.3 0.2 1 175 84 55 LEU N N 125.9 0.2 1 176 85 56 TYR H H 7.12 0.02 1 177 85 56 TYR CA C 53.8 0.2 1 178 85 56 TYR CB C 36.4 0.2 1 179 85 56 TYR N N 116.8 0.2 1 180 86 57 ALA H H 7.25 0.02 1 181 86 57 ALA CA C 46.4 0.2 1 182 86 57 ALA CB C 17.7 0.2 1 183 86 57 ALA N N 128.4 0.2 1 184 87 58 LYS H H 8.48 0.02 1 185 87 58 LYS CA C 50.2 0.2 1 186 87 58 LYS CB C 31.3 0.2 1 187 87 58 LYS N N 121.0 0.2 1 188 88 59 GLU H H 8.64 0.02 1 189 88 59 GLU CA C 51.1 0.2 1 190 88 59 GLU CB C 25.9 0.2 1 191 88 59 GLU N N 128.4 0.2 1 192 89 60 ASP H H 9.21 0.02 1 193 89 60 ASP CA C 50.1 0.2 1 194 89 60 ASP CB C 34.5 0.2 1 195 89 60 ASP N N 123.0 0.2 1 196 90 61 ALA H H 8.57 0.02 1 197 90 61 ALA CA C 47.7 0.2 1 198 90 61 ALA CB C 14.7 0.2 1 199 90 61 ALA N N 122.6 0.2 1 200 91 62 THR H H 8.96 0.02 1 201 91 62 THR CA C 56.8 0.2 1 202 91 62 THR CB C 66.3 0.2 1 203 91 62 THR N N 113.3 0.2 1 204 92 63 LYS H H 8.25 0.02 1 205 92 63 LYS CA C 49.6 0.2 1 206 92 63 LYS CB C 30.8 0.2 1 207 92 63 LYS N N 120.7 0.2 1 208 93 64 GLY H H 7.13 0.02 1 209 93 64 GLY CA C 40.8 0.2 1 210 93 64 GLY N N 111.0 0.2 1 211 94 65 LYS H H 8.23 0.02 1 212 94 65 LYS CA C 49.6 0.2 1 213 94 65 LYS CB C 32.7 0.2 1 214 94 65 LYS N N 118.1 0.2 1 215 95 66 PHE H H 7.97 0.02 1 216 95 66 PHE CA C 50.8 0.2 1 217 95 66 PHE CB C 37.3 0.2 1 218 95 66 PHE N N 115.1 0.2 1 219 96 67 ALA H H 8.09 0.02 1 220 96 67 ALA CA C 46.9 0.2 1 221 96 67 ALA N N 121.3 0.2 1 222 98 69 THR CA C 55.6 0.2 1 223 98 69 THR CB C 65.6 0.2 1 224 99 70 THR H H 8.87 0.02 1 225 99 70 THR CA C 56.3 0.2 1 226 99 70 THR CB C 65.4 0.2 1 227 99 70 THR N N 116.8 0.2 1 228 107 78 VAL CA C 56.1 0.2 1 229 107 78 VAL CB C 28.3 0.2 1 230 108 79 CYS H H 8.76 0.02 1 231 108 79 CYS CA C 50.5 0.2 1 232 108 79 CYS CB C 43.1 0.2 1 233 108 79 CYS N N 122.1 0.2 1 234 109 80 PHE H H 8.93 0.02 1 235 109 80 PHE CA C 51.2 0.2 1 236 109 80 PHE CB C 38.6 0.2 1 237 109 80 PHE N N 121.2 0.2 1 238 110 81 GLU H H 8.70 0.02 1 239 110 81 GLU CA C 49.8 0.2 1 240 110 81 GLU CB C 29.1 0.2 1 241 110 81 GLU N N 121.2 0.2 1 242 111 82 SER H H 8.84 0.02 1 243 111 82 SER CA C 50.2 0.2 1 244 111 82 SER CB C 63.3 0.2 1 245 111 82 SER N N 121.0 0.2 1 246 112 83 LYS H H 8.58 0.02 1 247 112 83 LYS CA C 50.0 0.2 1 248 112 83 LYS CB C 32.0 0.2 1 249 112 83 LYS N N 121.1 0.2 1 250 113 84 GLY H H 8.42 0.02 1 251 113 84 GLY CA C 40.5 0.2 1 252 113 84 GLY N N 110.3 0.2 1 253 114 85 THR H H 8.43 0.02 1 254 114 85 THR CA C 56.7 0.2 1 255 114 85 THR CB C 66.5 0.2 1 256 114 85 THR N N 114.5 0.2 1 257 115 86 GLY H H 8.14 0.02 1 258 115 86 GLY CA C 39.4 0.2 1 259 115 86 GLY N N 110.0 0.2 1 260 116 87 ARG H H 8.19 0.02 1 261 116 87 ARG CA C 50.8 0.2 1 262 116 87 ARG CB C 24.3 0.2 1 263 116 87 ARG N N 120.6 0.2 1 264 117 88 ILE H H 7.15 0.02 1 265 117 88 ILE CA C 53.3 0.2 1 266 117 88 ILE CB C 36.3 0.2 1 267 117 88 ILE N N 123.0 0.2 1 268 118 89 PRO CA C 57.9 0.2 1 269 118 89 PRO CB C 28.0 0.2 1 270 119 90 ASP H H 8.27 0.02 1 271 119 90 ASP CA C 50.2 0.2 1 272 119 90 ASP CB C 36.5 0.2 1 273 119 90 ASP N N 121.1 0.2 1 274 120 91 GLN H H 7.44 0.02 1 275 120 91 GLN CA C 50.7 0.2 1 276 120 91 GLN CB C 28.6 0.2 1 277 120 91 GLN N N 117.9 0.2 1 278 121 92 LEU H H 8.16 0.02 1 279 121 92 LEU CA C 49.2 0.2 1 280 121 92 LEU CB C 39.1 0.2 1 281 121 92 LEU N N 123.6 0.2 1 282 122 93 VAL H H 8.51 0.02 1 283 122 93 VAL CA C 56.2 0.2 1 284 122 93 VAL CB C 28.1 0.2 1 285 122 93 VAL N N 127.9 0.2 1 286 123 94 ILE H H 8.18 0.02 1 287 123 94 ILE CA C 55.3 0.2 1 288 123 94 ILE CB C 36.1 0.2 1 289 123 94 ILE N N 124.5 0.2 1 290 124 95 LEU H H 8.77 0.02 1 291 124 95 LEU CA C 48.9 0.2 1 292 124 95 LEU CB C 41.8 0.2 1 293 124 95 LEU N N 126.2 0.2 1 294 125 96 ASP H H 8.95 0.02 1 295 125 96 ASP CA C 48.5 0.2 1 296 125 96 ASP CB C 39.3 0.2 1 297 125 96 ASP N N 126.9 0.2 1 298 126 97 MET H H 8.47 0.02 1 299 126 97 MET CA C 50.0 0.2 1 300 126 97 MET CB C 39.2 0.2 1 301 126 97 MET N N 123.4 0.2 1 302 127 98 LYS H H 8.96 0.02 1 303 127 98 LYS CA C 50.2 0.2 1 304 127 98 LYS CB C 31.4 0.2 1 305 127 98 LYS N N 124.3 0.2 1 306 128 99 HIS H H 8.21 0.02 1 307 128 99 HIS CA C 49.2 0.2 1 308 128 99 HIS CB C 26.8 0.2 1 309 128 99 HIS N N 117.4 0.2 1 310 129 100 GLY H H 8.67 0.02 1 311 129 100 GLY CA C 40.6 0.2 1 312 129 100 GLY N N 109.7 0.2 1 313 130 101 VAL H H 7.99 0.02 1 314 130 101 VAL CA C 57.1 0.2 1 315 130 101 VAL CB C 28.5 0.2 1 316 130 101 VAL N N 118.1 0.2 1 317 131 102 GLU H H 8.23 0.02 1 318 131 102 GLU CA C 51.7 0.2 1 319 131 102 GLU CB C 25.9 0.2 1 320 131 102 GLU N N 124.1 0.2 1 321 132 103 ALA H H 7.77 0.02 1 322 132 103 ALA CA C 49.0 0.2 1 323 132 103 ALA CB C 15.8 0.2 1 324 132 103 ALA N N 130.8 0.2 1 stop_ save_