data_27777 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H-15N HSQC assignment for Insulin-like growth factor 2 mRNA-binding protein 1 domain KH1-2 ; _BMRB_accession_number 27777 _BMRB_flat_file_name bmr27777.str _Entry_type original _Submission_date 2019-02-07 _Accession_date 2019-02-07 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kelly Geoff . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 142 "15N chemical shifts" 142 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-06-26 update BMRB 'update entry citation' 2019-03-06 original author 'original release' stop_ _Original_release_date 2019-02-07 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; IMP1 KH1 and KH2 domains create a structural platform with unique RNA recognition and re-modelling properties. ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 30864660 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Dagil Robert . . 2 Ball Neil J. . 3 Ogrodowicz Roksana W. . 4 Hobor Fruzsina . . 5 Purkiss Andrew G. . 6 Kelly Geoff . . 7 Martin Stephen R. . 8 Taylor Ian A. . 9 Ramos Andres . . stop_ _Journal_abbreviation 'Nucleic Acids Res.' _Journal_name_full 'Nucleic acids research' _Journal_volume 47 _Journal_issue 8 _Journal_ISSN 1362-4962 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 4334 _Page_last 4348 _Year 2019 _Details . loop_ _Keyword 'IMP1 KH1-2' 'Multidomain RNA binding protein' 'Protein-RNA recognition' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Insulin-like growth factor 2 mRNA-binding protein 1 domain KH1-2' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'domain KH1-2' $IMP1_KH1-2 stop_ _System_molecular_weight 19.7 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_IMP1_KH1-2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common IMP1_KH1-2 _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'RNA binding protein' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 179 _Mol_residue_sequence ; GAMVDIPLRLLVPTQYVGAI IGKEGATIRNITKQTQSKID VHRKENAGAAEKAISVHSTP EGCSSACKMILEIMHKEAKD TKTADEVPLKILAHNNFVGR LIGKEGRNLKKVEQDTETKI TISSLQDLTLYNPERTITVK GAIENCCRAEQEIMKKVREA YENDVAAMSLQSHLIPGLN ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 191 GLY 2 192 ALA 3 193 MET 4 194 VAL 5 195 ASP 6 196 ILE 7 197 PRO 8 198 LEU 9 199 ARG 10 200 LEU 11 201 LEU 12 202 VAL 13 203 PRO 14 204 THR 15 205 GLN 16 206 TYR 17 207 VAL 18 208 GLY 19 209 ALA 20 210 ILE 21 211 ILE 22 212 GLY 23 213 LYS 24 214 GLU 25 215 GLY 26 216 ALA 27 217 THR 28 218 ILE 29 219 ARG 30 220 ASN 31 221 ILE 32 222 THR 33 223 LYS 34 224 GLN 35 225 THR 36 226 GLN 37 227 SER 38 228 LYS 39 229 ILE 40 230 ASP 41 231 VAL 42 232 HIS 43 233 ARG 44 234 LYS 45 235 GLU 46 236 ASN 47 237 ALA 48 238 GLY 49 239 ALA 50 240 ALA 51 241 GLU 52 242 LYS 53 243 ALA 54 244 ILE 55 245 SER 56 246 VAL 57 247 HIS 58 248 SER 59 249 THR 60 250 PRO 61 251 GLU 62 252 GLY 63 253 CYS 64 254 SER 65 255 SER 66 256 ALA 67 257 CYS 68 258 LYS 69 259 MET 70 260 ILE 71 261 LEU 72 262 GLU 73 263 ILE 74 264 MET 75 265 HIS 76 266 LYS 77 267 GLU 78 268 ALA 79 269 LYS 80 270 ASP 81 271 THR 82 272 LYS 83 273 THR 84 274 ALA 85 275 ASP 86 276 GLU 87 277 VAL 88 278 PRO 89 279 LEU 90 280 LYS 91 281 ILE 92 282 LEU 93 283 ALA 94 284 HIS 95 285 ASN 96 286 ASN 97 287 PHE 98 288 VAL 99 289 GLY 100 290 ARG 101 291 LEU 102 292 ILE 103 293 GLY 104 294 LYS 105 295 GLU 106 296 GLY 107 297 ARG 108 298 ASN 109 299 LEU 110 300 LYS 111 301 LYS 112 302 VAL 113 303 GLU 114 304 GLN 115 305 ASP 116 306 THR 117 307 GLU 118 308 THR 119 309 LYS 120 310 ILE 121 311 THR 122 312 ILE 123 313 SER 124 314 SER 125 315 LEU 126 316 GLN 127 317 ASP 128 318 LEU 129 319 THR 130 320 LEU 131 321 TYR 132 322 ASN 133 323 PRO 134 324 GLU 135 325 ARG 136 326 THR 137 327 ILE 138 328 THR 139 329 VAL 140 330 LYS 141 331 GLY 142 332 ALA 143 333 ILE 144 334 GLU 145 335 ASN 146 336 CYS 147 337 CYS 148 338 ARG 149 339 ALA 150 340 GLU 151 341 GLN 152 342 GLU 153 343 ILE 154 344 MET 155 345 LYS 156 346 LYS 157 347 VAL 158 348 ARG 159 349 GLU 160 350 ALA 161 351 TYR 162 352 GLU 163 353 ASN 164 354 ASP 165 355 VAL 166 356 ALA 167 357 ALA 168 358 MET 169 359 SER 170 360 LEU 171 361 GLN 172 362 SER 173 363 HIS 174 364 LEU 175 365 ILE 176 366 PRO 177 367 GLY 178 368 LEU 179 369 ASN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value UNP Q9NZI8 IGF2BP1 . . . . . stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $IMP1_KH1-2 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $IMP1_KH1-2 'recombinant technology' . Escherichia coli . pETM-30 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'pH 7.4' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $IMP1_KH1-2 560 uM '[U-100% 13C; U-100% 15N]' 'sodium phosphate' 10 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' TCEP 10 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CCPN_Analysis _Saveframe_category software _Name CCPN_Analysis _Version 2 loop_ _Vendor _Address _Electronic_address CCPN . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_950 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 950 _Details . save_ save_800 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ save_700 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_C(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D C(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCO_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCACB_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(CA)CO_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CA)CO' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.4 . pH pressure 1 . atm temperature 273 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 0 internal direct . . . 1.0 water N 15 protons ppm 0 internal direct . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CCPN_Analysis stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCA(CO)NH' '3D C(CO)NH' '3D HNCO' '3D HNCACB' '3D HN(CA)CO' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'domain KH1-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 194 4 VAL H H 8.149 . 1 2 194 4 VAL N N 120.208 . 1 3 195 5 ASP H H 8.272 . 1 4 195 5 ASP N N 124.167 . 1 5 196 6 ILE H H 7.843 . 1 6 196 6 ILE N N 120.665 . 1 7 198 8 LEU H H 7.964 . 1 8 198 8 LEU N N 120.081 . 1 9 199 9 ARG H H 9.149 . 1 10 199 9 ARG N N 129.616 . 1 11 200 10 LEU H H 8.832 . 1 12 200 10 LEU N N 121.856 . 1 13 201 11 LEU H H 8.005 . 1 14 201 11 LEU N N 120.302 . 1 15 202 12 VAL H H 9.192 . 1 16 202 12 VAL N N 121.468 . 1 17 204 14 THR H H 8.706 . 1 18 204 14 THR N N 122.660 . 1 19 206 16 TYR H H 7.641 . 1 20 206 16 TYR N N 116.571 . 1 21 207 17 VAL H H 7.603 . 1 22 207 17 VAL N N 120.884 . 1 23 208 18 GLY H H 8.679 . 1 24 208 18 GLY N N 106.957 . 1 25 209 19 ALA H H 7.650 . 1 26 209 19 ALA N N 124.515 . 1 27 210 20 ILE H H 7.369 . 1 28 210 20 ILE N N 118.117 . 1 29 211 21 ILE H H 7.662 . 1 30 211 21 ILE N N 114.597 . 1 31 212 22 GLY H H 8.108 . 1 32 212 22 GLY N N 106.005 . 1 33 215 25 GLY H H 7.766 . 1 34 215 25 GLY N N 105.151 . 1 35 216 26 ALA H H 7.841 . 1 36 216 26 ALA N N 120.040 . 1 37 217 27 THR H H 7.903 . 1 38 217 27 THR N N 115.687 . 1 39 218 28 ILE H H 8.034 . 1 40 218 28 ILE N N 124.529 . 1 41 219 29 ARG H H 7.958 . 1 42 219 29 ARG N N 123.189 . 1 43 220 30 ASN H H 7.607 . 1 44 220 30 ASN N N 118.410 . 1 45 221 31 ILE H H 8.456 . 1 46 221 31 ILE N N 119.216 . 1 47 222 32 THR H H 8.655 . 1 48 222 32 THR N N 114.823 . 1 49 223 33 LYS H H 7.925 . 1 50 223 33 LYS N N 121.926 . 1 51 224 34 GLN H H 8.298 . 1 52 224 34 GLN N N 115.804 . 1 53 225 35 THR H H 7.638 . 1 54 225 35 THR N N 104.995 . 1 55 226 36 GLN H H 7.613 . 1 56 226 36 GLN N N 118.718 . 1 57 227 37 SER H H 8.047 . 1 58 227 37 SER N N 114.036 . 1 59 228 38 LYS H H 8.457 . 1 60 228 38 LYS N N 120.485 . 1 61 229 39 ILE H H 8.386 . 1 62 229 39 ILE N N 124.711 . 1 63 230 40 ASP H H 8.979 . 1 64 230 40 ASP N N 127.827 . 1 65 231 41 VAL H H 8.366 . 1 66 231 41 VAL N N 122.435 . 1 67 232 42 HIS H H 8.717 . 1 68 232 42 HIS N N 127.505 . 1 69 240 50 ALA H H 7.918 . 1 70 240 50 ALA N N 122.859 . 1 71 241 51 GLU H H 7.562 . 1 72 241 51 GLU N N 117.772 . 1 73 242 52 LYS H H 9.056 . 1 74 242 52 LYS N N 119.314 . 1 75 243 53 ALA H H 8.694 . 1 76 243 53 ALA N N 124.972 . 1 77 244 54 ILE H H 9.106 . 1 78 244 54 ILE N N 120.026 . 1 79 245 55 SER H H 8.886 . 1 80 245 55 SER N N 120.300 . 1 81 246 56 VAL H H 9.544 . 1 82 246 56 VAL N N 125.070 . 1 83 247 57 HIS H H 9.105 . 1 84 247 57 HIS N N 125.779 . 1 85 248 58 SER H H 7.957 . 1 86 248 58 SER N N 118.161 . 1 87 249 59 THR H H 8.460 . 1 88 249 59 THR N N 109.268 . 1 89 251 61 GLU H H 8.822 . 1 90 251 61 GLU N N 115.505 . 1 91 252 62 GLY H H 8.220 . 1 92 252 62 GLY N N 112.629 . 1 93 253 63 CYS H H 8.836 . 1 94 253 63 CYS N N 119.603 . 1 95 254 64 SER H H 8.356 . 1 96 254 64 SER N N 117.154 . 1 97 255 65 SER H H 8.400 . 1 98 255 65 SER N N 119.444 . 1 99 256 66 ALA H H 8.624 . 1 100 256 66 ALA N N 125.120 . 1 101 257 67 CYS H H 8.475 . 1 102 257 67 CYS N N 117.403 . 1 103 258 68 LYS H H 7.561 . 1 104 258 68 LYS N N 118.130 . 1 105 259 69 MET H H 7.704 . 1 106 259 69 MET N N 115.696 . 1 107 260 70 ILE H H 8.499 . 1 108 260 70 ILE N N 119.542 . 1 109 261 71 LEU H H 8.848 . 1 110 261 71 LEU N N 121.082 . 1 111 262 72 GLU H H 8.657 . 1 112 262 72 GLU N N 119.855 . 1 113 263 73 ILE H H 8.006 . 1 114 263 73 ILE N N 121.563 . 1 115 264 74 MET H H 8.311 . 1 116 264 74 MET N N 117.926 . 1 117 265 75 HIS H H 8.420 . 1 118 265 75 HIS N N 116.444 . 1 119 266 76 LYS H H 7.856 . 1 120 266 76 LYS N N 120.311 . 1 121 267 77 GLU H H 7.989 . 1 122 267 77 GLU N N 119.334 . 1 123 268 78 ALA H H 8.290 . 1 124 268 78 ALA N N 124.244 . 1 125 269 79 LYS H H 7.771 . 1 126 269 79 LYS N N 117.762 . 1 127 270 80 ASP H H 8.798 . 1 128 270 80 ASP N N 121.210 . 1 129 271 81 THR H H 7.856 . 1 130 271 81 THR N N 107.712 . 1 131 272 82 LYS H H 7.967 . 1 132 272 82 LYS N N 119.030 . 1 133 273 83 THR H H 8.226 . 1 134 273 83 THR N N 110.430 . 1 135 274 84 ALA H H 7.752 . 1 136 274 84 ALA N N 122.908 . 1 137 275 85 ASP H H 8.290 . 1 138 275 85 ASP N N 117.729 . 1 139 276 86 GLU H H 7.728 . 1 140 276 86 GLU N N 117.354 . 1 141 277 87 VAL H H 8.147 . 1 142 277 87 VAL N N 122.672 . 1 143 279 89 LEU H H 8.416 . 1 144 279 89 LEU N N 123.419 . 1 145 280 90 LYS H H 8.810 . 1 146 280 90 LYS N N 127.590 . 1 147 281 91 ILE H H 9.123 . 1 148 281 91 ILE N N 124.013 . 1 149 282 92 LEU H H 8.490 . 1 150 282 92 LEU N N 126.695 . 1 151 283 93 ALA H H 8.579 . 1 152 283 93 ALA N N 122.060 . 1 153 284 94 HIS H H 9.011 . 1 154 284 94 HIS N N 124.712 . 1 155 285 95 ASN H H 8.332 . 1 156 285 95 ASN N N 124.285 . 1 157 287 97 PHE H H 8.238 . 1 158 287 97 PHE N N 116.351 . 1 159 288 98 VAL H H 7.228 . 1 160 288 98 VAL N N 111.593 . 1 161 291 101 LEU H H 7.681 . 1 162 291 101 LEU N N 119.608 . 1 163 292 102 ILE H H 7.722 . 1 164 292 102 ILE N N 112.595 . 1 165 293 103 GLY H H 7.696 . 1 166 293 103 GLY N N 106.661 . 1 167 299 109 LEU H H 7.626 . 1 168 299 109 LEU N N 121.913 . 1 169 300 110 LYS H H 7.604 . 1 170 300 110 LYS N N 117.409 . 1 171 301 111 LYS H H 7.472 . 1 172 301 111 LYS N N 119.932 . 1 173 302 112 VAL H H 7.624 . 1 174 302 112 VAL N N 118.750 . 1 175 303 113 GLU H H 8.308 . 1 176 303 113 GLU N N 122.825 . 1 177 304 114 GLN H H 7.978 . 1 178 304 114 GLN N N 117.769 . 1 179 305 115 ASP H H 8.771 . 1 180 305 115 ASP N N 119.122 . 1 181 306 116 THR H H 7.813 . 1 182 306 116 THR N N 105.635 . 1 183 307 117 GLU H H 7.852 . 1 184 307 117 GLU N N 119.767 . 1 185 308 118 THR H H 7.924 . 1 186 308 118 THR N N 108.603 . 1 187 309 119 LYS H H 8.194 . 1 188 309 119 LYS N N 117.518 . 1 189 310 120 ILE H H 10.136 . 1 190 310 120 ILE N N 128.163 . 1 191 311 121 THR H H 8.885 . 1 192 311 121 THR N N 118.855 . 1 193 312 122 ILE H H 8.663 . 1 194 312 122 ILE N N 121.583 . 1 195 313 123 SER H H 8.490 . 1 196 313 123 SER N N 123.487 . 1 197 317 127 ASP H H 7.504 . 1 198 317 127 ASP N N 117.742 . 1 199 318 128 LEU H H 7.567 . 1 200 318 128 LEU N N 121.849 . 1 201 319 129 THR H H 7.911 . 1 202 319 129 THR N N 112.690 . 1 203 322 132 ASN H H 7.366 . 1 204 322 132 ASN N N 121.795 . 1 205 324 134 GLU H H 7.616 . 1 206 324 134 GLU N N 119.059 . 1 207 325 135 ARG H H 8.782 . 1 208 325 135 ARG N N 121.656 . 1 209 326 136 THR H H 8.752 . 1 210 326 136 THR N N 116.612 . 1 211 327 137 ILE H H 10.095 . 1 212 327 137 ILE N N 133.444 . 1 213 328 138 THR H H 9.145 . 1 214 328 138 THR N N 124.800 . 1 215 329 139 VAL H H 9.802 . 1 216 329 139 VAL N N 129.279 . 1 217 330 140 LYS H H 8.835 . 1 218 330 140 LYS N N 124.668 . 1 219 331 141 GLY H H 8.104 . 1 220 331 141 GLY N N 110.498 . 1 221 332 142 ALA H H 8.320 . 1 222 332 142 ALA N N 122.085 . 1 223 333 143 ILE H H 8.656 . 1 224 333 143 ILE N N 122.421 . 1 225 334 144 GLU H H 9.210 . 1 226 334 144 GLU N N 118.028 . 1 227 335 145 ASN H H 7.317 . 1 228 335 145 ASN N N 118.635 . 1 229 336 146 CYS H H 8.649 . 1 230 336 146 CYS N N 118.041 . 1 231 337 147 CYS H H 7.944 . 1 232 337 147 CYS N N 117.480 . 1 233 338 148 ARG H H 7.732 . 1 234 338 148 ARG N N 120.538 . 1 235 339 149 ALA H H 8.102 . 1 236 339 149 ALA N N 121.009 . 1 237 340 150 GLU H H 8.492 . 1 238 340 150 GLU N N 120.469 . 1 239 341 151 GLN H H 7.692 . 1 240 341 151 GLN N N 117.320 . 1 241 342 152 GLU H H 7.562 . 1 242 342 152 GLU N N 117.984 . 1 243 343 153 ILE H H 9.064 . 1 244 343 153 ILE N N 120.707 . 1 245 344 154 MET H H 8.937 . 1 246 344 154 MET N N 115.187 . 1 247 345 155 LYS H H 7.715 . 1 248 345 155 LYS N N 121.350 . 1 249 346 156 LYS H H 7.320 . 1 250 346 156 LYS N N 117.486 . 1 251 347 157 VAL H H 8.452 . 1 252 347 157 VAL N N 123.631 . 1 253 348 158 ARG H H 8.672 . 1 254 348 158 ARG N N 117.756 . 1 255 349 159 GLU H H 7.989 . 1 256 349 159 GLU N N 118.914 . 1 257 350 160 ALA H H 7.733 . 1 258 350 160 ALA N N 121.719 . 1 259 351 161 TYR H H 8.061 . 1 260 351 161 TYR N N 119.014 . 1 261 352 162 GLU H H 8.279 . 1 262 352 162 GLU N N 117.556 . 1 263 353 163 ASN H H 7.872 . 1 264 353 163 ASN N N 116.004 . 1 265 354 164 ASP H H 8.142 . 1 266 354 164 ASP N N 121.235 . 1 267 355 165 VAL H H 8.003 . 1 268 355 165 VAL N N 120.089 . 1 269 356 166 ALA H H 8.102 . 1 270 356 166 ALA N N 124.390 . 1 271 357 167 ALA H H 7.889 . 1 272 357 167 ALA N N 120.693 . 1 273 358 168 MET H H 7.959 . 1 274 358 168 MET N N 116.999 . 1 275 364 174 LEU H H 7.819 . 1 276 364 174 LEU N N 122.061 . 1 277 365 175 ILE H H 7.930 . 1 278 365 175 ILE N N 122.896 . 1 279 367 177 GLY H H 8.354 . 1 280 367 177 GLY N N 109.071 . 1 281 368 178 LEU H H 7.934 . 1 282 368 178 LEU N N 121.572 . 1 283 369 179 ASN H H 7.899 . 1 284 369 179 ASN N N 124.146 . 1 stop_ save_