data_27905 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Clr4 1-191 NCP ; _BMRB_accession_number 27905 _BMRB_flat_file_name bmr27905.str _Entry_type original _Submission_date 2019-05-14 _Accession_date 2019-05-14 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Clr4 1-191 chemical shifts' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Akoury Elias . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 173 "15N chemical shifts" 173 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-12-10 update BMRB 'update entry citation' 2019-07-09 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 27903 'Clr4 1-191 free' stop_ _Original_release_date 2019-05-14 save_ ############################# # Citation for this entry # ############################# save_citations_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Disordered region of H3K9 methyltransferase Clr4 binds the nucleosome and contributes to its activity ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 31165882 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Akoury Elias . . 2 Ma Guoli . . 3 Demolin Segolene . . 4 Bronner Cornelia . . 5 Zocco Manuel . . 6 Cirilo Alexandre . . 7 Ivic Nives . . 8 Halic Mario . . stop_ _Journal_abbreviation 'Nucleic Acids Res.' _Journal_name_full 'Nucleic acids research' _Journal_volume 47 _Journal_issue 13 _Journal_ISSN 1362-4962 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 6726 _Page_last 6736 _Year 2019 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name clr4191 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label crr $protein stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_protein _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common protein _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 190 _Mol_residue_sequence ; MSPKQEEYEVERIVDEKLDR NGAVKLYRIRWLNYSSRSDT WEPPENLSGCSAVLAEWKRR KRRLKGSNSDSDSPHHASNP HPNSRQKHQHQTSKSVPRSQ RFSRELNVKKENKKVFSSQT TKRQSRKQSTALTTNDTSII LDDSLHTNSKKLGKTRNEVK EESQKRELVSNSIKEATSPK TSSILTKXRN ; loop_ _Residue_seq_code _Residue_label 1 MET 2 SER 3 PRO 4 LYS 5 GLN 6 GLU 7 GLU 8 TYR 9 GLU 10 VAL 11 GLU 12 ARG 13 ILE 14 VAL 15 ASP 16 GLU 17 LYS 18 LEU 19 ASP 20 ARG 21 ASN 22 GLY 23 ALA 24 VAL 25 LYS 26 LEU 27 TYR 28 ARG 29 ILE 30 ARG 31 TRP 32 LEU 33 ASN 34 TYR 35 SER 36 SER 37 ARG 38 SER 39 ASP 40 THR 41 TRP 42 GLU 43 PRO 44 PRO 45 GLU 46 ASN 47 LEU 48 SER 49 GLY 50 CYS 51 SER 52 ALA 53 VAL 54 LEU 55 ALA 56 GLU 57 TRP 58 LYS 59 ARG 60 ARG 61 LYS 62 ARG 63 ARG 64 LEU 65 LYS 66 GLY 67 SER 68 ASN 69 SER 70 ASP 71 SER 72 ASP 73 SER 74 PRO 75 HIS 76 HIS 77 ALA 78 SER 79 ASN 80 PRO 81 HIS 82 PRO 83 ASN 84 SER 85 ARG 86 GLN 87 LYS 88 HIS 89 GLN 90 HIS 91 GLN 92 THR 93 SER 94 LYS 95 SER 96 VAL 97 PRO 98 ARG 99 SER 100 GLN 101 ARG 102 PHE 103 SER 104 ARG 105 GLU 106 LEU 107 ASN 108 VAL 109 LYS 110 LYS 111 GLU 112 ASN 113 LYS 114 LYS 115 VAL 116 PHE 117 SER 118 SER 119 GLN 120 THR 121 THR 122 LYS 123 ARG 124 GLN 125 SER 126 ARG 127 LYS 128 GLN 129 SER 130 THR 131 ALA 132 LEU 133 THR 134 THR 135 ASN 136 ASP 137 THR 138 SER 139 ILE 140 ILE 141 LEU 142 ASP 143 ASP 144 SER 145 LEU 146 HIS 147 THR 148 ASN 149 SER 150 LYS 151 LYS 152 LEU 153 GLY 154 LYS 155 THR 156 ARG 157 ASN 158 GLU 159 VAL 160 LYS 161 GLU 162 GLU 163 SER 164 GLN 165 LYS 166 ARG 167 GLU 168 LEU 169 VAL 170 SER 171 ASN 172 SER 173 ILE 174 LYS 175 GLU 176 ALA 177 THR 178 SER 179 PRO 180 LYS 181 THR 182 SER 183 SER 184 ILE 185 LEU 186 THR 187 LYS 188 X 189 ARG 190 ASN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $protein 'E. coli' 562 Bacteria . Escherichia coli stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $protein 'recombinant technology' . Escherichia coli . bl21 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $protein 500 uM '[U-100% 13C; U-100% 15N]' stop_ save_ ############################ # Computer software used # ############################ save_CCPN _Saveframe_category software _Name CCPN _Version . loop_ _Vendor _Address _Electronic_address CCPN . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.8 . pH stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0 internal direct . . . 1.0 DSS N 15 nitrogen ppm 0 internal direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CCPN stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name crr _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 SER H H 8.37072 . . 2 2 2 SER N N 119.55836 . . 3 4 4 LYS H H 8.34217 . . 4 4 4 LYS N N 120.97348 . . 5 5 5 GLN H H 8.36756 . . 6 5 5 GLN N N 122.12881 . . 7 6 6 GLU H H 8.48641 . . 8 6 6 GLU N N 121.72022 . . 9 7 7 GLU H H 8.30438 . . 10 7 7 GLU N N 121.14055 . . 11 8 8 TYR H H 8.16862 . . 12 8 8 TYR N N 121.49236 . . 13 9 9 GLU H H 8.27276 . . 14 9 9 GLU N N 121.68912 . . 15 10 10 VAL H H 8.32418 . . 16 10 10 VAL N N 115.8471 . . 17 11 11 GLU H H 8.21686 . . 18 11 11 GLU N N 120.16151 . . 19 12 12 ARG H H 8.49136 . . 20 12 12 ARG N N 117.73523 . . 21 13 13 ILE H H 8.14566 . . 22 13 13 ILE N N 122.94639 . . 23 14 14 VAL H H 8.37288 . . 24 14 14 VAL N N 116.04643 . . 25 15 15 ASP H H 7.16785 . . 26 15 15 ASP N N 117.70345 . . 27 16 16 GLU H H 9.69398 . . 28 16 16 GLU N N 117.30896 . . 29 17 17 LYS H H 9.06641 . . 30 17 17 LYS N N 121.893 . . 31 18 18 LEU H H 9.05421 . . 32 18 18 LEU N N 125.52495 . . 33 19 19 ASP H H 9.11121 . . 34 19 19 ASP N N 121.28978 . . 35 20 20 ARG H H 8.53163 . . 36 20 20 ARG N N 118.04896 . . 37 21 21 ASN H H 8.31654 . . 38 21 21 ASN N N 116.73728 . . 39 22 22 GLY H H 8.16105 . . 40 22 22 GLY N N 109.02264 . . 41 23 23 ALA H H 8.24516 . . 42 23 23 ALA N N 123.34837 . . 43 24 24 VAL H H 8.50491 . . 44 24 24 VAL N N 121.14421 . . 45 25 25 LYS H H 9.28612 . . 46 25 25 LYS N N 127.22082 . . 47 26 26 LEU H H 7.38207 . . 48 26 26 LEU N N 116.03709 . . 49 27 27 TYR H H 9.442 . . 50 27 27 TYR N N 119.00438 . . 51 28 28 ARG H H 8.0097 . . 52 28 28 ARG N N 124.559 . . 53 29 29 ILE H H 8.41515 . . 54 29 29 ILE N N 126.37588 . . 55 30 30 ARG H H 8.08905 . . 56 30 30 ARG N N 124.02158 . . 57 31 31 TRP H H 8.30887 . . 58 31 31 TRP N N 122.39039 . . 59 32 32 LEU H H 8.29194 . . 60 32 32 LEU N N 125.0606 . . 61 33 33 ASN H H 8.78355 . . 62 33 33 ASN N N 116.75324 . . 63 34 34 TYR H H 8.3165 . . 64 34 34 TYR N N 118.60231 . . 65 35 35 SER H H 8.15377 . . 66 35 35 SER N N 115.712 . . 67 36 36 SER H H 8.21223 . . 68 36 36 SER N N 116.81635 . . 69 37 37 ARG H H 7.53513 . . 70 37 37 ARG N N 117.07199 . . 71 38 38 SER H H 8.25544 . . 72 38 38 SER N N 115.712 . . 73 39 39 ASP H H 8.39467 . . 74 39 39 ASP N N 122.24059 . . 75 40 40 THR H H 8.25153 . . 76 40 40 THR N N 110.88851 . . 77 41 41 TRP H H 8.7632 . . 78 41 41 TRP N N 120.88148 . . 79 42 42 GLU H H 9.66206 . . 80 42 42 GLU N N 122.94627 . . 81 45 45 GLU H H 9.83628 . . 82 45 45 GLU N N 118.49605 . . 83 46 46 ASN H H 8.03367 . . 84 46 46 ASN N N 119.69384 . . 85 47 47 LEU H H 7.84111 . . 86 47 47 LEU N N 121.5235 . . 87 48 48 SER H H 7.59049 . . 88 48 48 SER N N 116.16858 . . 89 49 49 GLY H H 8.95353 . . 90 49 49 GLY N N 111.2183 . . 91 50 50 CYS H H 8.08591 . . 92 50 50 CYS N N 120.39162 . . 93 51 51 SER H H 8.05459 . . 94 51 51 SER N N 116.83005 . . 95 52 52 ALA H H 8.824 . . 96 52 52 ALA N N 125.42761 . . 97 53 53 VAL H H 7.6421 . . 98 53 53 VAL N N 120.00166 . . 99 54 54 LEU H H 7.98425 . . 100 54 54 LEU N N 122.53035 . . 101 55 55 ALA H H 8.19758 . . 102 55 55 ALA N N 121.85098 . . 103 56 56 GLU H H 8.07147 . . 104 56 56 GLU N N 119.34292 . . 105 57 57 TRP H H 7.69003 . . 106 57 57 TRP N N 120.97728 . . 107 58 58 LYS H H 8.11312 . . 108 58 58 LYS N N 117.4478 . . 109 59 59 ARG H H 7.73548 . . 110 59 59 ARG N N 117.9861 . . 111 60 60 ARG H H 8.03026 . . 112 60 60 ARG N N 121.5447 . . 113 61 61 LYS H H 8.08811 . . 114 61 61 LYS N N 118.99519 . . 115 62 62 ARG H H 7.5953 . . 116 62 62 ARG N N 119.21074 . . 117 63 63 ARG H H 7.91274 . . 118 63 63 ARG N N 120.16343 . . 119 64 64 LEU H H 8.03754 . . 120 64 64 LEU N N 122.53801 . . 121 65 65 LYS H H 8.02593 . . 122 65 65 LYS N N 120.47152 . . 123 66 66 GLY H H 8.31601 . . 124 66 66 GLY N N 109.78689 . . 125 67 67 SER H H 8.24434 . . 126 67 67 SER N N 115.71346 . . 127 68 68 ASN H H 8.55828 . . 128 68 68 ASN N N 120.92771 . . 129 69 69 SER H H 7.89987 . . 130 69 69 SER N N 120.45348 . . 131 70 70 ASP H H 8.43324 . . 132 70 70 ASP N N 122.53124 . . 133 71 71 SER H H 8.15687 . . 134 71 71 SER N N 116.97996 . . 135 72 72 ASP H H 8.50751 . . 136 72 72 ASP N N 122.12534 . . 137 73 73 SER H H 8.14934 . . 138 73 73 SER N N 116.99942 . . 139 77 77 ALA H H 8.4443 . . 140 77 77 ALA N N 124.6146 . . 141 78 78 SER H H 8.21025 . . 142 78 78 SER N N 113.65432 . . 143 79 79 ASN H H 8.42346 . . 144 79 79 ASN N N 119.50613 . . 145 83 83 ASN H H 8.12135 . . 146 83 83 ASN N N 118.09931 . . 147 84 84 SER H H 8.44407 . . 148 84 84 SER N N 118.52626 . . 149 85 85 ARG H H 8.40417 . . 150 85 85 ARG N N 122.84425 . . 151 86 86 GLN H H 8.55259 . . 152 86 86 GLN N N 120.94666 . . 153 87 87 LYS H H 8.45734 . . 154 87 87 LYS N N 123.50146 . . 155 89 89 GLN H H 8.33648 . . 156 89 89 GLN N N 120.50244 . . 157 91 91 GLN H H 8.28018 . . 158 91 91 GLN N N 121.05569 . . 159 92 92 THR H H 8.10611 . . 160 92 92 THR N N 115.69282 . . 161 93 93 SER H H 8.41903 . . 162 93 93 SER N N 116.35674 . . 163 94 94 LYS H H 8.39772 . . 164 94 94 LYS N N 125.47282 . . 165 95 95 SER H H 8.32974 . . 166 95 95 SER N N 117.43735 . . 167 96 96 VAL H H 8.17117 . . 168 96 96 VAL N N 116.95224 . . 169 98 98 ARG H H 8.47853 . . 170 98 98 ARG N N 122.20243 . . 171 99 99 SER H H 8.27999 . . 172 99 99 SER N N 115.69821 . . 173 100 100 GLN H H 8.55412 . . 174 100 100 GLN N N 120.36001 . . 175 101 101 ARG H H 8.55812 . . 176 101 101 ARG N N 122.2052 . . 177 102 102 PHE H H 8.29134 . . 178 102 102 PHE N N 116.26841 . . 179 103 103 SER H H 8.05247 . . 180 103 103 SER N N 118.1305 . . 181 104 104 ARG H H 8.3793 . . 182 104 104 ARG N N 122.80624 . . 183 105 105 GLU H H 8.50143 . . 184 105 105 GLU N N 121.79009 . . 185 106 106 LEU H H 8.23692 . . 186 106 106 LEU N N 122.15381 . . 187 107 107 ASN H H 8.41809 . . 188 107 107 ASN N N 116.46771 . . 189 108 108 VAL H H 8.25538 . . 190 108 108 VAL N N 116.33636 . . 191 109 109 LYS H H 8.34879 . . 192 109 109 LYS N N 123.08355 . . 193 110 110 LYS H H 8.42867 . . 194 110 110 LYS N N 123.50093 . . 195 111 111 GLU H H 8.25034 . . 196 111 111 GLU N N 121.44952 . . 197 112 112 ASN H H 7.83716 . . 198 112 112 ASN N N 119.38129 . . 199 113 113 LYS H H 8.3296 . . 200 113 113 LYS N N 123.26017 . . 201 114 114 LYS H H 8.71476 . . 202 114 114 LYS N N 122.43459 . . 203 115 115 VAL H H 9.00039 . . 204 115 115 VAL N N 119.15577 . . 205 116 116 PHE H H 8.4246 . . 206 116 116 PHE N N 125.79199 . . 207 117 117 SER H H 8.05377 . . 208 117 117 SER N N 118.1409 . . 209 118 118 SER H H 8.44243 . . 210 118 118 SER N N 117.89077 . . 211 119 119 GLN H H 8.39098 . . 212 119 119 GLN N N 119.97083 . . 213 120 120 THR H H 8.19759 . . 214 120 120 THR N N 115.89411 . . 215 121 121 THR H H 8.23433 . . 216 121 121 THR N N 115.30531 . . 217 122 122 LYS H H 8.30839 . . 218 122 122 LYS N N 125.91501 . . 219 123 123 ARG H H 8.46258 . . 220 123 123 ARG N N 122.79506 . . 221 124 124 GLN H H 8.62414 . . 222 124 124 GLN N N 121.27644 . . 223 125 125 SER H H 8.38776 . . 224 125 125 SER N N 118.231 . . 225 126 126 ARG H H 8.4114 . . 226 126 126 ARG N N 123.08067 . . 227 127 127 LYS H H 8.41032 . . 228 127 127 LYS N N 122.46641 . . 229 128 128 GLN H H 8.35387 . . 230 128 128 GLN N N 122.09303 . . 231 129 129 SER H H 8.30879 . . 232 129 129 SER N N 117.97692 . . 233 130 130 THR H H 8.22528 . . 234 130 130 THR N N 114.5919 . . 235 131 131 ALA H H 8.31787 . . 236 131 131 ALA N N 126.54115 . . 237 132 132 LEU H H 8.52469 . . 238 132 132 LEU N N 122.34766 . . 239 133 133 THR H H 8.14369 . . 240 133 133 THR N N 113.22546 . . 241 134 134 THR H H 8.17143 . . 242 134 134 THR N N 115.23639 . . 243 135 135 ASN H H 8.32466 . . 244 135 135 ASN N N 119.73299 . . 245 136 136 ASP H H 8.27351 . . 246 136 136 ASP N N 125.34425 . . 247 137 137 THR H H 8.38165 . . 248 137 137 THR N N 109.21305 . . 249 138 138 SER H H 8.29898 . . 250 138 138 SER N N 116.34768 . . 251 139 139 ILE H H 8.09826 . . 252 139 139 ILE N N 121.84726 . . 253 140 140 ILE H H 8.24389 . . 254 140 140 ILE N N 125.98994 . . 255 141 141 LEU H H 8.36473 . . 256 141 141 LEU N N 124.34868 . . 257 142 142 ASP H H 8.30417 . . 258 142 142 ASP N N 123.6391 . . 259 143 143 ASP H H 8.14612 . . 260 143 143 ASP N N 123.05481 . . 261 144 144 SER H H 8.21874 . . 262 144 144 SER N N 117.49389 . . 263 145 145 LEU H H 8.34626 . . 264 145 145 LEU N N 125.5812 . . 265 147 147 THR H H 8.10681 . . 266 147 147 THR N N 114.97446 . . 267 148 148 ASN H H 8.4724 . . 268 148 148 ASN N N 119.89399 . . 269 149 149 SER H H 8.42813 . . 270 149 149 SER N N 118.53458 . . 271 150 150 LYS H H 8.39312 . . 272 150 150 LYS N N 125.3389 . . 273 151 151 LYS H H 8.41892 . . 274 151 151 LYS N N 123.51432 . . 275 152 152 LEU H H 8.37634 . . 276 152 152 LEU N N 123.57924 . . 277 153 153 GLY H H 8.40019 . . 278 153 153 GLY N N 109.79847 . . 279 154 154 LYS H H 8.33431 . . 280 154 154 LYS N N 122.55136 . . 281 155 155 THR H H 8.2214 . . 282 155 155 THR N N 114.75044 . . 283 156 156 ARG H H 8.48565 . . 284 156 156 ARG N N 123.41789 . . 285 157 157 ASN H H 8.30804 . . 286 157 157 ASN N N 119.09247 . . 287 158 158 GLU H H 8.43436 . . 288 158 158 GLU N N 121.64476 . . 289 160 160 LYS H H 8.57156 . . 290 160 160 LYS N N 122.49569 . . 291 161 161 GLU H H 8.27669 . . 292 161 161 GLU N N 121.61284 . . 293 162 162 GLU H H 8.37449 . . 294 162 162 GLU N N 121.33149 . . 295 163 163 SER H H 8.37068 . . 296 163 163 SER N N 115.40188 . . 297 164 164 GLN H H 8.33156 . . 298 164 164 GLN N N 120.73878 . . 299 165 165 LYS H H 7.81925 . . 300 165 165 LYS N N 120.40554 . . 301 166 166 ARG H H 8.62082 . . 302 166 166 ARG N N 121.16651 . . 303 167 167 GLU H H 8.52985 . . 304 167 167 GLU N N 121.54557 . . 305 168 168 LEU H H 8.23905 . . 306 168 168 LEU N N 122.82448 . . 307 169 169 VAL H H 8.24703 . . 308 169 169 VAL N N 121.15779 . . 309 170 170 SER H H 8.32598 . . 310 170 170 SER N N 119.23674 . . 311 171 171 ASN H H 8.4756 . . 312 171 171 ASN N N 119.83178 . . 313 172 172 SER H H 8.43178 . . 314 172 172 SER N N 118.78116 . . 315 173 173 ILE H H 8.27087 . . 316 173 173 ILE N N 122.53348 . . 317 174 174 LYS H H 8.35875 . . 318 174 174 LYS N N 125.6645 . . 319 175 175 GLU H H 8.30112 . . 320 175 175 GLU N N 121.92873 . . 321 176 176 ALA H H 8.42153 . . 322 176 176 ALA N N 127.68403 . . 323 177 177 THR H H 8.15043 . . 324 177 177 THR N N 113.61523 . . 325 178 178 SER H H 8.38421 . . 326 178 178 SER N N 116.20725 . . 327 180 180 LYS H H 8.43191 . . 328 180 180 LYS N N 121.54974 . . 329 181 181 THR H H 8.21841 . . 330 181 181 THR N N 114.74467 . . 331 182 182 SER H H 8.29587 . . 332 182 182 SER N N 116.12344 . . 333 183 183 SER H H 8.39622 . . 334 183 183 SER N N 116.85551 . . 335 184 184 ILE H H 8.15098 . . 336 184 184 ILE N N 122.39463 . . 337 185 185 LEU H H 8.21462 . . 338 185 185 LEU N N 123.71804 . . 339 186 186 THR H H 8.14904 . . 340 186 186 THR N N 113.23259 . . 341 187 187 LYS H H 8.31816 . . 342 187 187 LYS N N 125.92909 . . 343 189 189 ARG H H 8.44693 . . 344 189 189 ARG N N 121.95218 . . 345 190 190 ASN H H 8.20773 . . 346 190 190 ASN N N 118.97816 . . stop_ save_