data_27972 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; VSV Phosphoprotein 35-106 ; _BMRB_accession_number 27972 _BMRB_flat_file_name bmr27972.str _Entry_type original _Submission_date 2019-07-12 _Accession_date 2019-07-12 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Chemical shift data for VSV Phosphoprotein Residues 35 - 106' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Petit Chad M. . 2 Green Todd J. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 60 "13C chemical shifts" 114 "15N chemical shifts" 60 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-03-31 update BMRB 'update entry citation' 2020-02-11 original author 'original release' stop_ _Original_release_date 2019-07-12 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; The Connector Domain of Vesicular Stomatitis Virus Large Protein Interacts with the Viral Phosphoprotein ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 31896592 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Gould Joseph R. . 2 Qiu Shihong . . 3 Shang Qiao . . 4 Ogino Tomoaki . . 5 Prevelige Peter E. Jr 6 Petit Chad M. . 7 Green Todd J. . stop_ _Journal_abbreviation 'J. Virol.' _Journal_name_full 'Journal of virology' _Journal_volume 94 _Journal_issue 6 _Journal_ISSN 1098-5514 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first e01729-19 _Page_last e01729-19 _Year 2020 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'VSV Phosphoprotein 35-106' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'VSV Phosphoprotein 35-106' $L_Protein stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_L_Protein _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common L_Protein _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 72 _Mol_residue_sequence ; SNYELFQEDGVEEHTRPSYF QAADDSDTESEPEIEDNQGL YVPDPEAEQVEGFIQGPLDD YADEDVDVVFTS ; loop_ _Residue_seq_code _Residue_label 1 SER 2 ASN 3 TYR 4 GLU 5 LEU 6 PHE 7 GLN 8 GLU 9 ASP 10 GLY 11 VAL 12 GLU 13 GLU 14 HIS 15 THR 16 ARG 17 PRO 18 SER 19 TYR 20 PHE 21 GLN 22 ALA 23 ALA 24 ASP 25 ASP 26 SER 27 ASP 28 THR 29 GLU 30 SER 31 GLU 32 PRO 33 GLU 34 ILE 35 GLU 36 ASP 37 ASN 38 GLN 39 GLY 40 LEU 41 TYR 42 VAL 43 PRO 44 ASP 45 PRO 46 GLU 47 ALA 48 GLU 49 GLN 50 VAL 51 GLU 52 GLY 53 PHE 54 ILE 55 GLN 56 GLY 57 PRO 58 LEU 59 ASP 60 ASP 61 TYR 62 ALA 63 ASP 64 GLU 65 ASP 66 VAL 67 ASP 68 VAL 69 VAL 70 PHE 71 THR 72 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain _Gene_mnemonic _Details $L_Protein 'Vesicular Stomatitis Virus' 11276 Viruses . Vesiculovirus 'Vesicular Stomatitis Virus' Indiana Phosphoprotein 'Residues 35 - 106' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $L_Protein 'recombinant technology' . Escherichia coli . SpGFTk stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'Standard 15N (NH4Cl) and 13C (Glucose) labeling was used.' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $L_Protein 1 mM '[U-13C; U-15N]' HEPES 20 mM 'natural abundance' 'Sodium Chloride' 150 mM 'natural abundance' glycerol 10 % 'natural abundance' TCEP 1 mM 'natural abundance' 'sodium azide' 0.02 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_VNMRJ _Saveframe_category software _Name VNMRJ _Version . loop_ _Vendor _Address _Electronic_address Varian . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.150 . M pH 7.0 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCACB' '3D CBCA(CO)NH' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'VSV Phosphoprotein 35-106' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 4 4 GLU H H 8.249 0.020 1 2 4 4 GLU CA C 56.602 0.3 1 3 4 4 GLU CB C 30.214 0.3 1 4 4 4 GLU N N 121.896 0.3 1 5 5 5 LEU H H 8.004 0.020 1 6 5 5 LEU CA C 55.120 0.3 1 7 5 5 LEU CB C 42.412 0.3 1 8 5 5 LEU N N 122.422 0.3 1 9 6 6 PHE H H 8.168 0.020 1 10 6 6 PHE CA C 57.518 0.3 1 11 6 6 PHE CB C 39.720 0.3 1 12 6 6 PHE N N 120.672 0.3 1 13 7 7 GLN H H 8.180 0.020 1 14 7 7 GLN CA C 55.389 0.3 1 15 7 7 GLN CB C 29.810 0.3 1 16 7 7 GLN N N 122.385 0.3 1 17 8 8 GLU H H 8.428 0.020 1 18 8 8 GLU CA C 56.533 0.3 1 19 8 8 GLU CB C 30.163 0.3 1 20 8 8 GLU N N 122.784 0.3 1 21 10 10 GLY H H 8.368 0.020 1 22 10 10 GLY CA C 45.499 0.3 1 23 10 10 GLY N N 109.405 0.3 1 24 11 11 VAL H H 7.909 0.020 1 25 11 11 VAL CA C 62.318 0.3 1 26 11 11 VAL CB C 32.624 0.3 1 27 11 11 VAL N N 119.224 0.3 1 28 15 15 THR H H 8.044 0.020 1 29 15 15 THR CA C 61.911 0.3 1 30 15 15 THR CB C 69.641 0.3 1 31 15 15 THR N N 115.016 0.3 1 32 16 16 ARG H H 8.288 0.020 1 33 16 16 ARG CA C 54.041 0.3 1 34 16 16 ARG CB C 30.199 0.3 1 35 16 16 ARG N N 124.818 0.3 1 36 18 18 SER H H 8.353 0.020 1 37 18 18 SER CA C 58.382 0.3 1 38 18 18 SER CB C 63.675 0.3 1 39 18 18 SER N N 115.633 0.3 1 40 19 19 TYR H H 7.935 0.020 1 41 19 19 TYR CA C 57.592 0.3 1 42 19 19 TYR CB C 38.695 0.3 1 43 19 19 TYR N N 121.369 0.3 1 44 20 20 PHE H H 7.990 0.020 1 45 20 20 PHE CA C 57.598 0.3 1 46 20 20 PHE CB C 39.770 0.3 1 47 20 20 PHE N N 122.019 0.3 1 48 21 21 GLN H H 8.095 0.020 1 49 21 21 GLN CA C 55.284 0.3 1 50 21 21 GLN CB C 29.791 0.3 1 51 21 21 GLN N N 123.193 0.3 1 52 22 22 ALA H H 8.293 0.020 1 53 22 22 ALA CA C 52.293 0.3 1 54 22 22 ALA CB C 19.311 0.3 1 55 22 22 ALA N N 126.497 0.3 1 56 23 23 ALA H H 8.340 0.020 1 57 23 23 ALA CA C 52.312 0.3 1 58 23 23 ALA CB C 19.330 0.3 1 59 23 23 ALA N N 123.709 0.3 1 60 24 24 ASP H H 8.304 0.020 1 61 24 24 ASP CA C 54.239 0.3 1 62 24 24 ASP CB C 41.269 0.3 1 63 24 24 ASP N N 119.758 0.3 1 64 25 25 ASP H H 8.274 0.020 1 65 25 25 ASP CA C 54.294 0.3 1 66 25 25 ASP CB C 41.020 0.3 1 67 25 25 ASP N N 121.179 0.3 1 68 26 26 SER H H 8.270 0.020 1 69 26 26 SER CA C 58.853 0.3 1 70 26 26 SER CB C 63.924 0.3 1 71 26 26 SER N N 115.907 0.3 1 72 27 27 ASP H H 8.414 0.020 1 73 27 27 ASP CA C 54.349 0.3 1 74 27 27 ASP CB C 41.073 0.3 1 75 27 27 ASP N N 122.725 0.3 1 76 28 28 THR H H 8.073 0.020 1 77 28 28 THR CA C 61.997 0.3 1 78 28 28 THR CB C 69.817 0.3 1 79 28 28 THR N N 114.352 0.3 1 80 29 29 GLU H H 8.423 0.020 1 81 29 29 GLU CA C 56.373 0.3 1 82 29 29 GLU CB C 30.277 0.3 1 83 29 29 GLU N N 123.471 0.3 1 84 30 30 SER H H 8.293 0.020 1 85 30 30 SER CA C 58.184 0.3 1 86 30 30 SER CB C 63.982 0.3 1 87 30 30 SER N N 117.334 0.3 1 88 31 31 GLU H H 8.462 0.020 1 89 31 31 GLU CA C 54.396 0.3 1 90 31 31 GLU CB C 29.717 0.3 1 91 31 31 GLU N N 124.105 0.3 1 92 33 33 GLU H H 8.513 0.020 1 93 33 33 GLU CA C 56.339 0.3 1 94 33 33 GLU CB C 30.200 0.3 1 95 33 33 GLU N N 121.628 0.3 1 96 34 34 ILE H H 8.244 0.020 1 97 34 34 ILE CA C 60.838 0.3 1 98 34 34 ILE CB C 38.853 0.3 1 99 34 34 ILE N N 122.590 0.3 1 100 35 35 GLU H H 8.503 0.020 1 101 35 35 GLU CA C 56.505 0.3 1 102 35 35 GLU CB C 30.517 0.3 1 103 35 35 GLU N N 125.702 0.3 1 104 36 36 ASP H H 8.304 0.020 1 105 36 36 ASP CA C 54.245 0.3 1 106 36 36 ASP CB C 41.148 0.3 1 107 36 36 ASP N N 119.574 0.3 1 108 37 37 ASN H H 8.442 0.020 1 109 37 37 ASN CA C 53.172 0.3 1 110 37 37 ASN CB C 38.636 0.3 1 111 37 37 ASN N N 119.839 0.3 1 112 38 38 GLN H H 8.453 0.020 1 113 38 38 GLN CA C 56.449 0.3 1 114 38 38 GLN CB C 29.057 0.3 1 115 38 38 GLN N N 120.063 0.3 1 116 39 39 GLY H H 8.416 0.020 1 117 39 39 GLY CA C 45.400 0.3 1 118 39 39 GLY N N 109.273 0.3 1 119 40 40 LEU H H 7.906 0.020 1 120 40 40 LEU CA C 55.019 0.3 1 121 40 40 LEU CB C 42.432 0.3 1 122 40 40 LEU N N 121.384 0.3 1 123 41 41 TYR H H 8.215 0.020 1 124 41 41 TYR CA C 57.663 0.3 1 125 41 41 TYR CB C 38.656 0.3 1 126 41 41 TYR N N 122.152 0.3 1 127 42 42 VAL H H 7.954 0.020 1 128 42 42 VAL CA C 59.139 0.3 1 129 42 42 VAL CB C 33.204 0.3 1 130 42 42 VAL N N 126.620 0.3 1 131 44 44 ASP H H 8.330 0.020 1 132 44 44 ASP N N 121.643 0.3 1 133 46 46 GLU H H 8.414 0.020 1 134 46 46 GLU CA C 56.581 0.3 1 135 46 46 GLU CB C 29.817 0.3 1 136 46 46 GLU N N 119.438 0.3 1 137 47 47 ALA H H 7.964 0.020 1 138 47 47 ALA CA C 52.591 0.3 1 139 47 47 ALA CB C 19.288 0.3 1 140 47 47 ALA N N 124.109 0.3 1 141 48 48 GLU H H 8.247 0.020 1 142 48 48 GLU CA C 56.601 0.3 1 143 48 48 GLU CB C 30.295 0.3 1 144 48 48 GLU N N 119.814 0.3 1 145 49 49 GLN H H 8.330 0.020 1 146 49 49 GLN CA C 55.483 0.3 1 147 49 49 GLN CB C 29.282 0.3 1 148 49 49 GLN N N 121.772 0.3 1 149 50 50 VAL H H 8.182 0.020 1 150 50 50 VAL CA C 62.162 0.3 1 151 50 50 VAL CB C 32.843 0.3 1 152 50 50 VAL N N 121.729 0.3 1 153 51 51 GLU H H 8.522 0.020 1 154 51 51 GLU CA C 56.743 0.3 1 155 51 51 GLU CB C 30.199 0.3 1 156 51 51 GLU N N 124.781 0.3 1 157 52 52 GLY H H 8.421 0.020 1 158 52 52 GLY CA C 45.140 0.3 1 159 52 52 GLY N N 110.395 0.3 1 160 53 53 PHE H H 8.034 0.020 1 161 53 53 PHE CA C 57.807 0.3 1 162 53 53 PHE CB C 39.708 0.3 1 163 53 53 PHE N N 120.352 0.3 1 164 54 54 ILE H H 8.028 0.020 1 165 54 54 ILE CA C 60.567 0.3 1 166 54 54 ILE CB C 38.837 0.3 1 167 54 54 ILE N N 124.417 0.3 1 168 55 55 GLN H H 8.396 0.020 1 169 55 55 GLN CA C 55.789 0.3 1 170 55 55 GLN CB C 29.722 0.3 1 171 55 55 GLN N N 125.556 0.3 1 172 56 56 GLY H H 8.351 0.020 1 173 56 56 GLY CA C 44.425 0.3 1 174 56 56 GLY N N 111.900 0.3 1 175 58 58 LEU H H 8.633 0.020 1 176 58 58 LEU CA C 54.769 0.3 1 177 58 58 LEU CB C 42.165 0.3 1 178 58 58 LEU N N 123.086 0.3 1 179 59 59 ASP H H 8.172 0.020 1 180 59 59 ASP CA C 54.343 0.3 1 181 59 59 ASP CB C 41.245 0.3 1 182 59 59 ASP N N 120.963 0.3 1 183 60 60 ASP H H 8.161 0.020 1 184 60 60 ASP CA C 54.131 0.3 1 185 60 60 ASP CB C 41.062 0.3 1 186 60 60 ASP N N 120.229 0.3 1 187 61 61 TYR H H 8.077 0.020 1 188 61 61 TYR CA C 57.852 0.3 1 189 61 61 TYR CB C 38.602 0.3 1 190 61 61 TYR N N 120.560 0.3 1 191 62 62 ALA H H 8.061 0.020 1 192 62 62 ALA CA C 52.226 0.3 1 193 62 62 ALA CB C 19.632 0.3 1 194 62 62 ALA N N 126.073 0.3 1 195 63 63 ASP H H 8.194 0.020 1 196 63 63 ASP CA C 54.276 0.3 1 197 63 63 ASP CB C 41.184 0.3 1 198 63 63 ASP N N 120.240 0.3 1 199 64 64 GLU H H 8.317 0.020 1 200 64 64 GLU CA C 56.516 0.3 1 201 64 64 GLU CB C 30.536 0.3 1 202 64 64 GLU N N 120.946 0.3 1 203 65 65 ASP H H 8.430 0.020 1 204 65 65 ASP CA C 54.349 0.3 1 205 65 65 ASP CB C 41.073 0.3 1 206 65 65 ASP N N 121.471 0.3 1 207 66 66 VAL H H 7.944 0.020 1 208 66 66 VAL CA C 62.103 0.3 1 209 66 66 VAL CB C 32.882 0.3 1 210 66 66 VAL N N 119.497 0.3 1 211 67 67 ASP H H 8.384 0.020 1 212 67 67 ASP CA C 54.416 0.3 1 213 67 67 ASP CB C 41.131 0.3 1 214 67 67 ASP N N 123.746 0.3 1 215 68 68 VAL H H 7.949 0.020 1 216 68 68 VAL CA C 62.306 0.3 1 217 68 68 VAL CB C 32.663 0.3 1 218 68 68 VAL N N 120.675 0.3 1 219 69 69 VAL H H 8.098 0.020 1 220 69 69 VAL CA C 62.122 0.3 1 221 69 69 VAL CB C 32.805 0.3 1 222 69 69 VAL N N 124.112 0.3 1 223 70 70 PHE H H 8.417 0.020 1 224 70 70 PHE CA C 57.617 0.3 1 225 70 70 PHE CB C 39.736 0.3 1 226 70 70 PHE N N 124.872 0.3 1 227 71 71 THR H H 8.113 0.020 1 228 71 71 THR CA C 61.438 0.3 1 229 71 71 THR CB C 70.011 0.3 1 230 71 71 THR N N 116.451 0.3 1 231 72 72 SER H H 7.884 0.020 1 232 72 72 SER CA C 60.014 0.3 1 233 72 72 SER CB C 64.742 0.3 1 234 72 72 SER N N 123.350 0.3 1 stop_ save_