data_28033 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone assignments for the Asciz QT2-4/LC8 complex ; _BMRB_accession_number 28033 _BMRB_flat_file_name bmr28033.str _Entry_type original _Submission_date 2019-10-24 _Accession_date 2019-10-24 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Reardon Patrick . . 2 Barbar Elisar . . 3 Jara Kayla . . 4 Smith Delaney . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 53 "13C chemical shifts" 149 "15N chemical shifts" 53 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-04-17 update BMRB 'update entry citation' 2020-03-05 original author 'original release' stop_ _Original_release_date 2019-10-25 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; The dynein light chain 8 (LC8) binds predominantly "in-register" to a multivalent intrinsically disordered partner ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 32139510 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Reardon Patrick N. . 2 Jara Kayla A. . 3 Rolland Amber D. . 4 Smith Delaney A. . 5 Hoang Hanh . . 6 Prell James S. . 7 Barbar Elisar J. . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_name_full 'The Journal of biological chemistry' _Journal_volume 295 _Journal_issue 15 _Journal_ISSN 1083-351X _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 4912 _Page_last 4922 _Year 2020 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'LC8/QT2-4 complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'QT2-4, chain 1' $QT2-4 'QT2-4, chain 2' $QT2-4 'LC8, chain 1-1' $LC8 'LC8, chain 1-2' $LC8 'LC8, chain 2-1' $LC8 'LC8, chain 2-2' $LC8 'LC8, chain 3-1' $LC8 'LC8, chain 4-2' $LC8 stop_ _System_molecular_weight . _System_physical_state 'partially disordered' _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details 'The predominate complex under these conditions is 2 QT2-4 chains and 3 LC8 homodimers.' save_ ######################## # Monomeric polymers # ######################## save_QT2-4 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common QT2-4 _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 76 _Mol_residue_sequence ; GAMHMNEVLAPLLRDIETQT PDTRGDIGTMTDDFPEEQEP VAVGSHFHAYSETEPMFDLQ TSAHMYTQTCDDLFEE ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 269 GLY 2 270 ALA 3 271 MET 4 272 HIS 5 273 MET 6 274 ASN 7 275 GLU 8 276 VAL 9 277 LEU 10 278 ALA 11 279 PRO 12 280 LEU 13 281 LEU 14 282 ARG 15 283 ASP 16 284 ILE 17 285 GLU 18 286 THR 19 287 GLN 20 288 THR 21 289 PRO 22 290 ASP 23 291 THR 24 292 ARG 25 293 GLY 26 294 ASP 27 295 ILE 28 296 GLY 29 297 THR 30 298 MET 31 299 THR 32 300 ASP 33 301 ASP 34 302 PHE 35 303 PRO 36 304 GLU 37 305 GLU 38 306 GLN 39 307 GLU 40 308 PRO 41 309 VAL 42 310 ALA 43 311 VAL 44 312 GLY 45 313 SER 46 314 HIS 47 315 PHE 48 316 HIS 49 317 ALA 50 318 TYR 51 319 SER 52 320 GLU 53 321 THR 54 322 GLU 55 323 PRO 56 324 MET 57 325 PHE 58 326 ASP 59 327 LEU 60 328 GLN 61 329 THR 62 330 SER 63 331 ALA 64 332 HIS 65 333 MET 66 334 TYR 67 335 THR 68 336 GLN 69 337 THR 70 338 CYS 71 339 ASP 72 340 ASP 73 341 LEU 74 342 PHE 75 343 GLU 76 344 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ save_LC8 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common LC8 _Molecular_mass . _Mol_thiol_state 'all free' _Details . _Residue_count 92 _Mol_residue_sequence ; GAHMSDRKAVIKNADMSEEM QQDAVDCATQALEKYNIEKD IAAYIKKEFDKKYNPTWHCI VGRNFGSYVTHETRHFIYFY LGQVAILLFKSG ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 ALA 3 HIS 4 MET 5 SER 6 ASP 7 ARG 8 LYS 9 ALA 10 VAL 11 ILE 12 LYS 13 ASN 14 ALA 15 ASP 16 MET 17 SER 18 GLU 19 GLU 20 MET 21 GLN 22 GLN 23 ASP 24 ALA 25 VAL 26 ASP 27 CYS 28 ALA 29 THR 30 GLN 31 ALA 32 LEU 33 GLU 34 LYS 35 TYR 36 ASN 37 ILE 38 GLU 39 LYS 40 ASP 41 ILE 42 ALA 43 ALA 44 TYR 45 ILE 46 LYS 47 LYS 48 GLU 49 PHE 50 ASP 51 LYS 52 LYS 53 TYR 54 ASN 55 PRO 56 THR 57 TRP 58 HIS 59 CYS 60 ILE 61 VAL 62 GLY 63 ARG 64 ASN 65 PHE 66 GLY 67 SER 68 TYR 69 VAL 70 THR 71 HIS 72 GLU 73 THR 74 ARG 75 HIS 76 PHE 77 ILE 78 TYR 79 PHE 80 TYR 81 LEU 82 GLY 83 GLN 84 VAL 85 ALA 86 ILE 87 LEU 88 LEU 89 PHE 90 LYS 91 SER 92 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $QT2-4 'fruit fly' 7227 Eukaryota Metazoa Drosophila melanogaster $LC8 'fruit fly' 7227 Eukaryota Metazoa Drosophila melanogaster stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $QT2-4 'recombinant technology' . Escherichia coli . pET2Zt2-1a $LC8 'recombinant technology' . Escherichia coli . pET2Zt2-1a stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $QT2-4 0.5 mM '[U-13C; U-15N; U-2H]' $LC8 2.0 mM 'natural abundance' beta-mercaptoethanol 10 mM 'natural abundance' 'sodium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' 'sodium azide' 1 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_CARA _Saveframe_category software _Name CARA _Version . loop_ _Vendor _Address _Electronic_address 'Keller and Wuthrich' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCO_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HCACO_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCACO' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . pH pressure 1 . atm temperature 273 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $NMRPipe $NMRView $CARA stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D HNCO' '3D HNCA' '3D HNCACB' '3D HN(CO)CA' '3D CBCA(CO)NH' '3D HCACO' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'QT2-4, chain 1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 273 5 MET H H 8.1720 0.0000 1 2 273 5 MET C C 175.9770 0.0000 1 3 273 5 MET CA C 55.2720 0.0000 1 4 273 5 MET CB C 31.5260 0.0000 1 5 273 5 MET N N 120.6950 0.0000 1 6 274 6 ASN H H 8.3180 0.0000 1 7 274 6 ASN C C 174.9270 0.0000 1 8 274 6 ASN CA C 53.3350 0.0000 1 9 274 6 ASN CB C 38.2210 0.0000 1 10 274 6 ASN N N 119.4190 0.0000 1 11 275 7 GLU H H 8.1430 0.0000 1 12 275 7 GLU C C 176.0620 0.0000 1 13 275 7 GLU CA C 56.2860 0.0000 1 14 275 7 GLU CB C 29.2400 0.0000 1 15 275 7 GLU N N 121.2630 0.0000 1 16 276 8 VAL H H 7.9600 0.0000 1 17 276 8 VAL C C 175.7780 0.0000 1 18 276 8 VAL CA C 61.9740 0.0000 1 19 276 8 VAL CB C 31.5070 0.0000 1 20 276 8 VAL N N 121.5460 0.0000 1 21 277 9 LEU H H 8.0770 0.0000 1 22 277 9 LEU C C 176.2670 0.0000 1 23 277 9 LEU CA C 54.3800 0.0000 1 24 277 9 LEU CB C 41.2420 0.0000 1 25 277 9 LEU N N 126.2260 0.0000 1 26 278 10 ALA H H 7.9780 0.0000 1 27 278 10 ALA C C 175.0370 0.0000 1 28 278 10 ALA CA C 49.8920 0.0000 1 29 278 10 ALA CB C 17.4030 0.0000 1 30 278 10 ALA N N 126.3060 0.0000 1 31 279 11 PRO CA C 62.4050 0.0000 1 32 279 11 PRO CB C 31.2000 0.0000 1 33 280 12 LEU H H 8.2540 0.0000 1 34 280 12 LEU C C 176.1610 0.0000 1 35 280 12 LEU CA C 55.1180 0.0000 1 36 280 12 LEU CB C 41.3230 0.0000 1 37 280 12 LEU N N 122.9820 0.0000 1 38 281 13 LEU H H 7.7020 0.0000 1 39 281 13 LEU C C 176.6010 0.0000 1 40 281 13 LEU CA C 52.8890 0.0000 1 41 281 13 LEU CB C 42.9560 0.0000 1 42 281 13 LEU N N 122.0330 0.0000 1 43 282 14 ARG H H 8.4080 0.0000 1 44 282 14 ARG C C 173.0960 0.0000 1 45 282 14 ARG CA C 54.2730 0.0000 1 46 282 14 ARG CB C 32.8330 0.0000 1 47 282 14 ARG N N 121.6940 0.0000 1 48 283 15 ASP H H 7.9220 0.0000 1 49 283 15 ASP C C 175.6930 0.0000 1 50 283 15 ASP CA C 52.3660 0.0000 1 51 283 15 ASP CB C 40.7520 0.0000 1 52 283 15 ASP N N 123.6810 0.0000 1 53 284 16 ILE H H 8.7300 0.0000 1 54 284 16 ILE C C 170.0880 0.0000 1 55 284 16 ILE CA C 57.6850 0.0000 1 56 284 16 ILE CB C 41.6500 0.0000 1 57 284 16 ILE N N 121.6520 0.0000 1 58 285 17 GLU H H 7.0990 0.0000 1 59 285 17 GLU C C 175.4090 0.0000 1 60 285 17 GLU CA C 52.0280 0.0000 1 61 285 17 GLU CB C 32.0980 0.0000 1 62 285 17 GLU N N 120.0460 0.0000 1 63 286 18 THR H H 7.9850 0.0000 1 64 286 18 THR CA C 60.0990 0.0000 1 65 286 18 THR CB C 70.5850 0.0000 1 66 286 18 THR N N 111.0120 0.0000 1 67 287 19 GLN H H 7.6820 0.0000 1 68 287 19 GLN CA C 55.3950 0.0000 1 69 287 19 GLN CB C 31.5600 0.0000 1 70 287 19 GLN N N 120.2960 0.0000 1 71 293 25 GLY H H 8.0970 0.0000 1 72 293 25 GLY CA C 43.8960 0.0000 1 73 293 25 GLY N N 107.9310 0.0000 1 74 294 26 ASP H H 7.8610 0.0000 1 75 294 26 ASP CA C 52.0280 0.0000 1 76 294 26 ASP CB C 41.4690 0.0000 1 77 294 26 ASP N N 119.9240 0.0000 1 78 295 27 ILE H H 8.5790 0.0000 1 79 295 27 ILE C C 170.3860 0.0000 1 80 295 27 ILE CA C 58.9310 0.0000 1 81 295 27 ILE CB C 41.1040 0.0000 1 82 295 27 ILE N N 122.9290 0.0000 1 83 296 28 GLY H H 7.4830 0.0000 1 84 296 28 GLY CA C 44.0500 0.0000 1 85 296 28 GLY N N 109.9180 0.0000 1 86 297 29 THR CA C 59.6380 0.0000 1 87 297 29 THR CB C 71.1560 0.0000 1 88 298 30 MET H H 8.0350 0.0000 1 89 298 30 MET C C 177.8640 0.0000 1 90 298 30 MET CA C 53.7960 0.0000 1 91 298 30 MET CB C 35.6420 0.0000 1 92 298 30 MET N N 119.3940 0.0000 1 93 299 31 THR H H 7.7010 0.0000 1 94 299 31 THR CA C 60.7750 0.0000 1 95 299 31 THR CB C 68.7070 0.0000 1 96 299 31 THR N N 117.2590 0.0000 1 97 300 32 ASP H H 7.9060 0.0000 1 98 300 32 ASP CA C 53.7350 0.0000 1 99 300 32 ASP CB C 41.3570 0.0000 1 100 300 32 ASP N N 120.7290 0.0000 1 101 303 35 PRO CA C 62.8040 0.0000 1 102 303 35 PRO CB C 30.9550 0.0000 1 103 304 36 GLU H H 8.3510 0.0000 1 104 304 36 GLU CA C 56.2560 0.0000 1 105 304 36 GLU CB C 29.3220 0.0000 1 106 304 36 GLU N N 121.3690 0.0000 1 107 305 37 GLU H H 8.3010 0.0000 1 108 305 37 GLU C C 175.0800 0.0000 1 109 305 37 GLU CA C 56.0710 0.0000 1 110 305 37 GLU CB C 29.4040 0.0000 1 111 305 37 GLU N N 122.0780 0.0000 1 112 306 38 GLN H H 8.2220 0.0000 1 113 306 38 GLN C C 175.5080 0.0000 1 114 306 38 GLN CA C 55.0870 0.0000 1 115 306 38 GLN CB C 29.0770 0.0000 1 116 306 38 GLN N N 121.6100 0.0000 1 117 307 39 GLU H H 8.3010 0.0000 1 118 307 39 GLU C C 174.4160 0.0000 1 119 307 39 GLU CA C 54.0420 0.0000 1 120 307 39 GLU CB C 28.7970 0.0000 1 121 307 39 GLU N N 123.8600 0.0000 1 122 308 40 PRO CA C 62.7430 0.0000 1 123 308 40 PRO CB C 30.9410 0.0000 1 124 309 41 VAL H H 7.9920 0.0000 1 125 309 41 VAL C C 175.6930 0.0000 1 126 309 41 VAL CA C 61.7280 0.0000 1 127 309 41 VAL CB C 31.5260 0.0000 1 128 309 41 VAL N N 120.2700 0.0000 1 129 310 42 ALA H H 8.1810 0.0000 1 130 310 42 ALA C C 177.4100 0.0000 1 131 310 42 ALA CA C 51.7670 0.0000 1 132 310 42 ALA CB C 18.3010 0.0000 1 133 310 42 ALA N N 127.7420 0.0000 1 134 311 43 VAL H H 7.9500 0.0000 1 135 311 43 VAL C C 176.5590 0.0000 1 136 311 43 VAL CA C 62.0050 0.0000 1 137 311 43 VAL CB C 31.7710 0.0000 1 138 311 43 VAL N N 119.8180 0.0000 1 139 312 44 GLY H H 8.3030 0.0000 1 140 312 44 GLY C C 173.9900 0.0000 1 141 312 44 GLY CA C 44.8190 0.0000 1 142 312 44 GLY N N 112.1860 0.0000 1 143 313 45 SER H H 8.0160 0.0000 1 144 313 45 SER N N 115.6300 0.0000 1 145 318 50 TYR H H 8.0070 0.0000 1 146 318 50 TYR C C 175.6220 0.0000 1 147 318 50 TYR CA C 57.6520 0.0000 1 148 318 50 TYR CB C 37.8940 0.0000 1 149 318 50 TYR N N 119.7910 0.0000 1 150 319 51 SER H H 7.9930 0.0000 1 151 319 51 SER C C 175.5510 0.0000 1 152 319 51 SER CA C 57.6520 0.0000 1 153 319 51 SER CB C 63.5300 0.0000 1 154 319 51 SER N N 117.4680 0.0000 1 155 320 52 GLU H H 8.3060 0.0000 1 156 320 52 GLU C C 176.4170 0.0000 1 157 320 52 GLU CA C 56.4400 0.0000 1 158 320 52 GLU CB C 29.2400 0.0000 1 159 320 52 GLU N N 123.0090 0.0000 1 160 321 53 THR H H 7.9380 0.0000 1 161 321 53 THR C C 174.0890 0.0000 1 162 321 53 THR CA C 61.3290 0.0000 1 163 321 53 THR CB C 69.3260 0.0000 1 164 321 53 THR N N 113.8340 0.0000 1 165 322 54 GLU H H 8.0820 0.0000 1 166 322 54 GLU CA C 53.9810 0.0000 1 167 322 54 GLU CB C 28.8320 0.0000 1 168 322 54 GLU N N 124.5240 0.0000 1 169 323 55 PRO CA C 62.8770 0.0000 1 170 323 55 PRO CB C 30.8730 0.0000 1 171 324 56 MET H H 8.1390 0.0000 1 172 324 56 MET CA C 54.9570 0.0000 1 173 324 56 MET CB C 31.8530 0.0000 1 174 324 56 MET N N 120.2220 0.0000 1 175 325 57 PHE H H 7.9330 0.0000 1 176 325 57 PHE CA C 57.0550 0.0000 1 177 325 57 PHE CB C 38.9560 0.0000 1 178 325 57 PHE N N 120.7480 0.0000 1 179 326 58 ASP H H 8.2170 0.0000 1 180 326 58 ASP CA C 53.7000 0.0000 1 181 326 58 ASP CB C 40.4020 0.0000 1 182 326 58 ASP N N 121.8320 0.0000 1 183 327 59 LEU H H 7.9500 0.0000 1 184 327 59 LEU C C 177.0410 0.0000 1 185 327 59 LEU CA C 54.9340 0.0000 1 186 327 59 LEU CB C 41.0780 0.0000 1 187 327 59 LEU N N 123.0090 0.0000 1 188 328 60 GLN H H 8.3080 0.0000 1 189 328 60 GLN C C 176.4170 0.0000 1 190 328 60 GLN CA C 55.6410 0.0000 1 191 328 60 GLN CB C 28.3420 0.0000 1 192 328 60 GLN N N 120.8550 0.0000 1 193 329 61 THR H H 8.0870 0.0000 1 194 329 61 THR C C 174.2310 0.0000 1 195 329 61 THR CA C 61.4210 0.0000 1 196 329 61 THR CB C 69.1630 0.0000 1 197 329 61 THR N N 113.6750 0.0000 1 198 330 62 SER H H 7.7760 0.0000 1 199 330 62 SER C C 171.8190 0.0000 1 200 330 62 SER CA C 56.6860 0.0000 1 201 330 62 SER CB C 65.3260 0.0000 1 202 330 62 SER N N 117.6370 0.0000 1 203 331 63 ALA H H 8.2010 0.0000 1 204 331 63 ALA C C 173.6500 0.0000 1 205 331 63 ALA CA C 50.2910 0.0000 1 206 331 63 ALA CB C 21.1920 0.0000 1 207 331 63 ALA N N 123.7530 0.0000 1 208 332 64 HIS H H 7.5800 0.0000 1 209 332 64 HIS C C 176.7150 0.0000 1 210 332 64 HIS CA C 53.2430 0.0000 1 211 332 64 HIS CB C 31.2000 0.0000 1 212 332 64 HIS N N 118.1690 0.0000 1 213 333 65 MET H H 8.9100 0.0000 1 214 333 65 MET C C 172.7700 0.0000 1 215 333 65 MET CA C 52.5510 0.0000 1 216 333 65 MET CB C 34.7440 0.0000 1 217 333 65 MET N N 122.8780 0.0000 1 218 334 66 TYR H H 7.3100 0.0000 1 219 334 66 TYR C C 176.0620 0.0000 1 220 334 66 TYR CA C 54.9800 0.0000 1 221 334 66 TYR CB C 41.5200 0.0000 1 222 334 66 TYR N N 118.5250 0.0000 1 223 335 67 THR H H 8.1440 0.0000 1 224 335 67 THR C C 172.0040 0.0000 1 225 335 67 THR CA C 60.6520 0.0000 1 226 335 67 THR CB C 70.7480 0.0000 1 227 335 67 THR N N 110.0580 0.0000 1 228 336 68 GLN H H 7.8130 0.0000 1 229 336 68 GLN CA C 55.2720 0.0000 1 230 336 68 GLN CB C 31.2340 0.0000 1 231 336 68 GLN N N 121.2270 0.0000 1 232 340 72 ASP H H 8.1590 0.0000 1 233 340 72 ASP CA C 54.3800 0.0000 1 234 340 72 ASP CB C 39.9690 0.0000 1 235 340 72 ASP N N 119.3660 0.0000 1 236 341 73 LEU H H 7.6490 0.0000 1 237 341 73 LEU C C 176.5870 0.0000 1 238 341 73 LEU CA C 55.1800 0.0000 1 239 341 73 LEU CB C 40.7550 0.0000 1 240 341 73 LEU N N 121.3860 0.0000 1 241 342 74 PHE H H 7.5720 0.0000 1 242 342 74 PHE C C 175.3380 0.0000 1 243 342 74 PHE CA C 56.9320 0.0000 1 244 342 74 PHE CB C 38.6290 0.0000 1 245 342 74 PHE N N 118.5410 0.0000 1 246 343 75 GLU H H 7.8690 0.0000 1 247 343 75 GLU C C 175.0830 0.0000 1 248 343 75 GLU CA C 55.9480 0.0000 1 249 343 75 GLU CB C 29.8940 0.0000 1 250 343 75 GLU N N 122.4370 0.0000 1 251 344 76 GLU H H 7.8520 0.0000 1 252 344 76 GLU C C 180.8580 0.0000 1 253 344 76 GLU CA C 57.6390 0.0000 1 254 344 76 GLU CB C 30.2200 0.0000 1 255 344 76 GLU N N 127.4020 0.0000 1 stop_ save_