data_30503 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; MPER-TM Domain of HIV-1 envelope glycoprotein (Env) ; _BMRB_accession_number 30503 _BMRB_flat_file_name bmr30503.str _Entry_type original _Submission_date 2018-07-31 _Accession_date 2018-07-31 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Fu Q. . . 2 Shaik M. M. . 3 Cai Y. . . 4 Ghantous F. . . 5 Piai A. . . 6 Peng H. . . 7 Rits-Volloch S. . . 8 Liu Z. . . 9 Harrison S. C. . 10 Seaman M. S. . 11 Chen B. . . 12 Chou J. J. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 49 "13C chemical shifts" 88 "15N chemical shifts" 49 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-10-03 update author 'update chemical shifts' 2019-07-09 update BMRB 'update entry citation' 2018-08-31 original author 'original release' stop_ _Original_release_date 2018-08-28 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structure of the membrane proximal external region of HIV-1 envelope glycoprotein ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 30185554 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Fu Q. . . 2 Shaik M. M. . 3 Cai Y. . . 4 Ghantous F. . . 5 Piai A. . . 6 Peng H. . . 7 Rits-Volloch S. . . 8 Liu Z. . . 9 Harrison S. C. . 10 Seaman M. S. . 11 Chen B. . . 12 Chou J. J. . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.' _Journal_volume 115 _Journal_issue 38 _Journal_ASTM PNASA6 _Journal_ISSN 1091-6490 _Journal_CSD 0040 _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first E8892 _Page_last E8899 _Year 2018 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Envelope glycoprotein gp160' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'entity_1, 1' $entity_1 'entity_1, 2' $entity_1 'entity_1, 3' $entity_1 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common entity_1 _Molecular_mass 6179.373 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 51 _Mol_residue_sequence ; LLELDKWASLWNWFDITNWL WYIRIFIIIVGSLIGLRIVF AVLSLVNRVRQ ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 660 LEU 2 661 LEU 3 662 GLU 4 663 LEU 5 664 ASP 6 665 LYS 7 666 TRP 8 667 ALA 9 668 SER 10 669 LEU 11 670 TRP 12 671 ASN 13 672 TRP 14 673 PHE 15 674 ASP 16 675 ILE 17 676 THR 18 677 ASN 19 678 TRP 20 679 LEU 21 680 TRP 22 681 TYR 23 682 ILE 24 683 ARG 25 684 ILE 26 685 PHE 27 686 ILE 28 687 ILE 29 688 ILE 30 689 VAL 31 690 GLY 32 691 SER 33 692 LEU 34 693 ILE 35 694 GLY 36 695 LEU 37 696 ARG 38 697 ILE 39 698 VAL 40 699 PHE 41 700 ALA 42 701 VAL 43 702 LEU 44 703 SER 45 704 LEU 46 705 VAL 47 706 ASN 48 707 ARG 49 708 VAL 50 709 ARG 51 710 GLN stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Gene_mnemonic $entity_1 HIV-1 11676 Viruses . Lentivirus HIV-1 env stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $entity_1 'recombinant technology' . . . BL21 plasmid 'pMM - LR6' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type bicelle _Details '15N, 2H Envelope glycoprotein gp160, 50 mM DMPC, 100 mM DHPC, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling DMPC 50 mM 'natural abundance' DHPC 100 mM 'natural abundance' $entity_1 . mM '15N, 2H' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type bicelle _Details '15N, 2H, 13C Envelope glycoprotein gp160, 50 mM DMPC, 100 mM DHPC, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling DMPC 50 mM 'natural abundance' DHPC 100 mM 'natural abundance' $entity_1 . mM '[U-13C; U-15N; U-2H]' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type bicelle _Details '15N, 13C Envelope glycoprotein gp160, 50 mM [U-99% 2H] DMPC, 100 mM [U-99% 2H] DHPC, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling DMPC 50 mM '[U-99% 2H]' DHPC 100 mM '[U-99% 2H]' $entity_1 . mM '[U-100% 13C; U-100% 15N]' stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type bicelle _Details '15N, 2H mixed with 13C(15%) Envelope glycoprotein gp160, 50 mM [U-99% 2H] DMPC, 100 mM [U-99% 2H] DHPC, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling DMPC 50 mM '[U-99% 2H]' DHPC 100 mM '[U-99% 2H]' $entity_1 . mM '15N, 2H mixed with 13C(15%)' stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type bicelle _Details '15N, 2H mixed with 13C Envelope glycoprotein gp160, 50 mM [U-99% 2H] DMPC, 100 mM [U-99% 2H] DHPC, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling DMPC 50 mM '[U-99% 2H]' DHPC 100 mM '[U-99% 2H]' $entity_1 . mM '15N, 2H mixed with 13C' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name XEASY _Version . loop_ _Vendor _Address _Electronic_address 'Bartels et al.' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_software_2 _Saveframe_category software _Name 'X-PLOR NIH' _Version 2.48 loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task refinement 'structure calculation' stop_ _Details . save_ save_software_3 _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_software_4 _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AvanceII _Field_strength 800 _Details . save_ save_NMR_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AvanceIII _Field_strength 900 _Details . save_ save_NMR_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AvanceII _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_Trosy_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N Trosy HSQC' _Sample_label $sample_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_2 save_ save_3D_HN(CO)CA_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_2 save_ save_3D_HNCO_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_2 save_ save_3D_1H-15N_NOESY_tr-HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY tr-HSQC' _Sample_label $sample_3 save_ save_3D_Jch_Modulated_1H-15N_NOESY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D Jch Modulated 1H-15N NOESY' _Sample_label $sample_5 save_ save_3D_1H-13C_NOESY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_4 save_ save_3D_1H-15N_NOESY_tr-HSQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY tr-HSQC' _Sample_label $sample_4 save_ save_3D_1H-13C_NOESY_9 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_3 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.7 . pH pressure 1 . atm temperature 308 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.000 internal indirect . . . 0.25144953 DSS H 1 'methyl protons' ppm 0.000 internal direct . . . 1.0 DSS N 15 'methyl protons' ppm 0.000 internal indirect . . . 0.10132912 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N Trosy HSQC' '3D HNCA' '3D HN(CO)CA' '3D HNCO' '3D 1H-15N NOESY tr-HSQC' '3D Jch Modulated 1H-15N NOESY' '3D 1H-13C NOESY' stop_ loop_ _Sample_label $sample_1 $sample_2 $sample_3 $sample_5 $sample_4 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name 'entity_1, 1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 661 2 LEU H H 8.51 0.0022 1 2 661 2 LEU C C 176.67 0.0300 1 3 661 2 LEU CA C 54.99 0.0500 1 4 661 2 LEU N N 122.17 0.0200 1 5 662 3 GLU H H 8.45 0.0022 1 6 662 3 GLU C C 176.55 0.0300 1 7 662 3 GLU N N 122.43 0.0200 1 8 663 4 LEU H H 8.24 0.0022 1 9 663 4 LEU C C 177.54 0.0300 1 10 663 4 LEU CA C 56.02 0.0500 1 11 663 4 LEU N N 122.75 0.0200 1 12 664 5 ASP H H 8.39 0.0022 1 13 664 5 ASP C C 176.16 0.0300 1 14 664 5 ASP CA C 55.09 0.0500 1 15 664 5 ASP N N 118.12 0.0200 1 16 665 6 LYS H H 7.82 0.0022 1 17 665 6 LYS C C 177.61 0.0300 1 18 665 6 LYS CA C 57.30 0.0500 1 19 665 6 LYS N N 120.10 0.0200 1 20 666 7 TRP H H 7.72 0.0022 1 21 666 7 TRP CA C 58.16 0.0500 1 22 666 7 TRP N N 119.99 0.0200 1 23 667 8 ALA H H 7.85 0.0022 1 24 667 8 ALA C C 179.05 0.0300 1 25 667 8 ALA CA C 54.13 0.0500 1 26 667 8 ALA N N 120.98 0.0200 1 27 668 9 SER H H 7.81 0.0022 1 28 668 9 SER C C 175.75 0.0300 1 29 668 9 SER CA C 60.17 0.0500 1 30 668 9 SER N N 112.49 0.0200 1 31 669 10 LEU H H 7.62 0.0022 1 32 669 10 LEU C C 177.46 0.0300 1 33 669 10 LEU CA C 56.78 0.0500 1 34 669 10 LEU N N 122.15 0.0200 1 35 670 11 TRP H H 7.51 0.0022 1 36 670 11 TRP C C 176.96 0.0300 1 37 670 11 TRP CA C 57.86 0.0500 1 38 670 11 TRP N N 116.59 0.0200 1 39 671 12 ASN H H 7.84 0.0022 1 40 671 12 ASN C C 177.42 0.0300 1 41 671 12 ASN CA C 54.15 0.0500 1 42 671 12 ASN N N 116.53 0.0200 1 43 672 13 TRP H H 7.51 0.0022 1 44 672 13 TRP C C 174.66 0.0300 1 45 672 13 TRP CA C 57.30 0.0500 1 46 672 13 TRP N N 117.78 0.0200 1 47 673 14 PHE H H 7.69 0.0022 1 48 673 14 PHE C C 175.54 0.0300 1 49 673 14 PHE CA C 52.97 0.0500 1 50 673 14 PHE N N 119.65 0.0200 1 51 674 15 ASP C C 179.38 0.0300 1 52 674 15 ASP CA C 58.51 0.0500 1 53 675 16 ILE H H 7.92 0.0022 1 54 675 16 ILE C C 176.43 0.0300 1 55 675 16 ILE CA C 65.19 0.0500 1 56 675 16 ILE N N 119.81 0.0200 1 57 676 17 THR H H 8.05 0.0022 1 58 676 17 THR C C 177.08 0.0300 1 59 676 17 THR CA C 65.11 0.0500 1 60 676 17 THR N N 115.18 0.0200 1 61 677 18 ASN H H 8.11 0.0022 1 62 677 18 ASN C C 177.62 0.0300 1 63 677 18 ASN CA C 59.97 0.0500 1 64 677 18 ASN N N 122.09 0.0200 1 65 678 19 TRP H H 7.89 0.0022 1 66 678 19 TRP C C 177.22 0.0300 1 67 678 19 TRP CA C 63.50 0.0500 1 68 678 19 TRP N N 119.72 0.0200 1 69 679 20 LEU H H 7.83 0.0022 1 70 679 20 LEU C C 177.62 0.0300 1 71 679 20 LEU CA C 61.51 0.0500 1 72 679 20 LEU N N 120.42 0.0200 1 73 680 21 TRP H H 7.80 0.0022 1 74 680 21 TRP N N 120.43 0.0200 1 75 681 22 TYR H H 7.56 0.0022 1 76 681 22 TYR N N 115.69 0.0200 1 77 682 23 ILE H H 8.07 0.0022 1 78 682 23 ILE C C 177.64 0.0300 1 79 682 23 ILE CA C 64.53 0.0500 1 80 682 23 ILE N N 118.21 0.0200 1 81 683 24 ARG H H 8.08 0.0022 1 82 683 24 ARG CA C 59.52 0.0500 1 83 683 24 ARG N N 119.89 0.0200 1 84 684 25 ILE H H 7.66 0.0022 1 85 684 25 ILE CA C 62.39 0.0500 1 86 684 25 ILE N N 117.13 0.0200 1 87 685 26 PHE H H 8.19 0.0022 1 88 685 26 PHE CA C 61.16 0.0500 1 89 685 26 PHE N N 120.36 0.0200 1 90 686 27 ILE H H 8.35 0.0022 1 91 686 27 ILE C C 177.62 0.0300 1 92 686 27 ILE CA C 64.38 0.0500 1 93 686 27 ILE N N 118.16 0.0200 1 94 687 28 ILE H H 8.01 0.0022 1 95 687 28 ILE C C 178.02 0.0300 1 96 687 28 ILE CA C 64.96 0.0500 1 97 687 28 ILE N N 120.23 0.0200 1 98 688 29 ILE H H 8.43 0.0022 1 99 688 29 ILE C C 177.03 0.0300 1 100 688 29 ILE CA C 65.49 0.0500 1 101 688 29 ILE N N 122.18 0.0200 1 102 689 30 VAL H H 8.34 0.0022 1 103 689 30 VAL C C 177.26 0.0300 1 104 689 30 VAL CA C 66.75 0.0500 1 105 689 30 VAL N N 118.60 0.0200 1 106 690 31 GLY H H 8.80 0.0022 1 107 690 31 GLY C C 175.12 0.0300 1 108 690 31 GLY CA C 46.80 0.0500 1 109 690 31 GLY N N 106.71 0.0200 1 110 691 32 SER H H 8.06 0.0022 1 111 691 32 SER C C 175.26 0.0300 1 112 691 32 SER CA C 62.94 0.0500 1 113 691 32 SER N N 118.23 0.0200 1 114 692 33 LEU H H 7.86 0.0022 1 115 692 33 LEU CA C 57.82 0.0500 1 116 692 33 LEU N N 121.90 0.0200 1 117 693 34 ILE H H 8.12 0.0022 1 118 693 34 ILE CA C 64.24 0.0500 1 119 693 34 ILE N N 118.76 0.0200 1 120 694 35 GLY H H 8.53 0.0022 1 121 694 35 GLY C C 174.81 0.0300 1 122 694 35 GLY CA C 47.25 0.0500 1 123 694 35 GLY N N 107.14 0.0200 1 124 695 36 LEU H H 8.40 0.0022 1 125 695 36 LEU C C 177.94 0.0300 1 126 695 36 LEU CA C 57.38 0.0500 1 127 695 36 LEU N N 121.23 0.0200 1 128 696 37 ARG H H 7.96 0.0022 1 129 696 37 ARG C C 178.76 0.0300 1 130 696 37 ARG CA C 58.54 0.0500 1 131 696 37 ARG N N 119.05 0.0200 1 132 697 38 ILE H H 8.17 0.0022 1 133 697 38 ILE C C 177.19 0.0300 1 134 697 38 ILE CA C 65.04 0.0500 1 135 697 38 ILE N N 120.35 0.0200 1 136 698 39 VAL H H 8.23 0.0022 1 137 698 39 VAL C C 177.52 0.0300 1 138 698 39 VAL CA C 66.98 0.0500 1 139 698 39 VAL N N 120.08 0.0200 1 140 699 40 PHE H H 8.40 0.0022 1 141 699 40 PHE C C 177.91 0.0300 1 142 699 40 PHE CA C 61.31 0.0500 1 143 699 40 PHE N N 117.63 0.0200 1 144 700 41 ALA H H 8.08 0.0022 1 145 700 41 ALA C C 180.29 0.0300 1 146 700 41 ALA CA C 54.61 0.0500 1 147 700 41 ALA N N 123.21 0.0200 1 148 701 42 VAL H H 8.28 0.0022 1 149 701 42 VAL C C 177.59 0.0300 1 150 701 42 VAL CA C 66.27 0.0500 1 151 701 42 VAL N N 118.50 0.0200 1 152 702 43 LEU H H 8.28 0.0022 1 153 702 43 LEU C C 178.66 0.0300 1 154 702 43 LEU CA C 57.38 0.0500 1 155 702 43 LEU N N 119.03 0.0200 1 156 703 44 SER H H 7.90 0.0022 1 157 703 44 SER C C 176.08 0.0300 1 158 703 44 SER CA C 61.06 0.0500 1 159 703 44 SER N N 113.65 0.0200 1 160 704 45 LEU H H 7.57 0.0022 1 161 704 45 LEU C C 178.53 0.0300 1 162 704 45 LEU CA C 56.98 0.0500 1 163 704 45 LEU N N 121.72 0.0200 1 164 705 46 VAL H H 7.86 0.0022 1 165 705 46 VAL C C 176.60 0.0300 1 166 705 46 VAL CA C 64.53 0.0500 1 167 705 46 VAL N N 116.27 0.0200 1 168 706 47 ASN H H 7.80 0.0022 1 169 706 47 ASN C C 175.49 0.0300 1 170 706 47 ASN CA C 54.06 0.0500 1 171 706 47 ASN N N 117.02 0.0200 1 172 707 48 ARG H H 7.60 0.0022 1 173 707 48 ARG C C 176.55 0.0300 1 174 707 48 ARG CA C 56.30 0.0500 1 175 707 48 ARG N N 118.75 0.0200 1 176 708 49 VAL H H 7.67 0.0022 1 177 708 49 VAL C C 174.97 0.0300 1 178 708 49 VAL CA C 61.96 0.0500 1 179 708 49 VAL N N 117.75 0.0200 1 180 709 50 ARG H H 7.50 0.0022 1 181 709 50 ARG C C 176.73 0.0300 1 182 709 50 ARG CA C 56.62 0.0500 1 183 709 50 ARG N N 103.83 0.0200 1 184 710 51 GLN H H 7.95 0.0022 1 185 710 51 GLN CA C 57.73 0.0500 1 186 710 51 GLN N N 121.89 0.0200 1 stop_ save_