data_34484 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Constitutive decay element CDE2 from human 3'UTR ; _BMRB_accession_number 34484 _BMRB_flat_file_name bmr34484.str _Entry_type original _Submission_date 2020-01-27 _Accession_date 2020-01-27 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Schwalbe H. . . 2 Binas O. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 159 "13C chemical shifts" 94 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2020-05-25 original BMRB . stop_ _Original_release_date 2020-05-22 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structural basis for the recognition of transiently structured AU-rich elements by Roquin ; _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Binas O. . . 2 Tants J. N. . 3 Peter S. . . 4 Janowski R. . . 5 Davydova E. . . 6 Braun J. . . 7 Niessing D. . . 8 Schwalbe H. . . 9 Weigand J. E. . 10 Schlundt A. . . stop_ _Journal_abbreviation 'Nucleic Acids Res.' _Journal_volume . _Journal_issue . _Journal_ASTM NARHAD _Journal_ISSN 1362-4962 _Journal_CSD 0389 _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year 2020 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name "RNA (5'-R(*GP*GP*UP*GP*CP*CP*UP*AP*AP*UP*AP*UP*UP*UP*AP*GP*GP*CP*AP*CP*C)-3')" _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label unit_1 $entity_1 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common entity_1 _Molecular_mass 6690.004 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 21 _Mol_residue_sequence ; GGUGCCUAAUAUUUAGGCAC C ; loop_ _Residue_seq_code _Residue_label 1 G 2 G 3 U 4 G 5 C 6 C 7 U 8 A 9 A 10 U 11 A 12 U 13 U 14 U 15 A 16 G 17 G 18 C 19 A 20 C 21 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 'Thermosinus carboxydivorans Nor1' 401526 Bacteria . Thermosinus carboxydivorans stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity_1 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '900 uM CDE2, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 900 uM 'natural abundance' KCl 50 mM 'natural abundance' 'potassium phosphate buffer' 25 mM 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details '1300 uM [U-99% 13C; U-99% 15N] CDE2, 100% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1300 uM '[U-99% 13C; U-99% 15N]' KCl 50 mM 'natural abundance' 'potassium phosphate buffer' 25 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name CNS _Version 1.2 loop_ _Vendor _Address _Electronic_address 'Brunger A. T. et.al.' . . stop_ loop_ _Task 'structure calculation' stop_ _Details . save_ save_software_2 _Saveframe_category software _Name Sparky _Version 3.2 loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model 'AVANCE III' _Field_strength 600 _Details . save_ save_NMR_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model 'AVANCE III' _Field_strength 800 _Details . save_ save_NMR_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model 'AVANCE III' _Field_strength 900 _Details . save_ save_NMR_spectrometer_4 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model 'AVANCE III' _Field_strength 950 _Details . save_ save_NMR_spectrometer_5 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model 'AVANCE III' _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1 save_ save_2D_gamma_HCN_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D gamma HCN' _Sample_label $sample_2 save_ save_3D_HCN_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCN' _Sample_label $sample_2 save_ save_fwHCCH-TOCSY-CCH-E.COSY_5 _Saveframe_category NMR_applied_experiment _Experiment_name fwHCCH-TOCSY-CCH-E.COSY _Sample_label $sample_2 save_ save_3D_HCP_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCP' _Sample_label $sample_2 save_ save_2D_qHCP_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D qHCP' _Sample_label $sample_2 save_ save_HCCH-TOCSY_8 _Saveframe_category NMR_applied_experiment _Experiment_name HCCH-TOCSY _Sample_label $sample_2 save_ save_2D_PFIDS_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D PFIDS' _Sample_label $sample_2 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 75 . mM pH 6.2 . pH pressure 1 . bar temperature 298 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 75 . mM pH 6.2 . pH pressure 1 . bar temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details ; 13C 15N referencing was calculated using the au program calc_wishart.rw from Bruker Topspin 3.5 developed by Ruediger Weisemann (Bruker Biospin) after Wishart et al. ; loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.000 internal direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '2D 1H-13C HSQC' '2D gamma HCN' '3D HCN' fwHCCH-TOCSY-CCH-E.COSY '3D HCP' '2D qHCP' HCCH-TOCSY '2D PFIDS' stop_ loop_ _Sample_label $sample_1 $sample_2 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name unit_1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 G H1' H 5.843 0.02 . 2 1 1 G H2' H 4.961 0.02 . 3 1 1 G H3' H 4.695 0.02 . 4 1 1 G H4' H 4.572 0.02 . 5 1 1 G H5' H 4.458 0.02 . 6 1 1 G H5'' H 4.284 0.02 . 7 1 1 G H8 H 8.154 0.02 . 8 1 1 G C1' C 91.893 0.02 . 9 1 1 G C2' C 75.074 0.02 . 10 1 1 G C3' C 74.591 0.02 . 11 1 1 G C4' C 83.275 0.02 . 12 1 1 G C5' C 67.161 0.02 . 13 2 2 G H1' H 5.936 0.02 . 14 2 2 G H2' H 4.526 0.02 . 15 2 2 G H3' H 4.559 0.02 . 16 2 2 G H4' H 4.493 0.02 . 17 2 2 G H5' H 4.411 0.02 . 18 2 2 G H5'' H 4.242 0.02 . 19 2 2 G H8 H 7.541 0.02 . 20 2 2 G C1' C 93.094 0.02 . 21 2 2 G C3' C 73.108 0.02 . 22 2 2 G C5' C 66.829 0.02 . 23 3 3 U H1' H 5.591 0.02 . 24 3 3 U H2' H 4.631 0.02 . 25 3 3 U H3' H 4.594 0.02 . 26 3 3 U H4' H 4.475 0.02 . 27 3 3 U H5 H 5.141 0.02 . 28 3 3 U H5' H 4.605 0.02 . 29 3 3 U H5'' H 4.140 0.02 . 30 3 3 U H6 H 7.784 0.02 . 31 3 3 U C1' C 93.552 0.02 . 32 3 3 U C2' C 75.378 0.02 . 33 3 3 U C3' C 72.352 0.02 . 34 3 3 U C4' C 82.065 0.02 . 35 3 3 U C5' C 64.503 0.02 . 36 4 4 G H1' H 5.825 0.02 . 37 4 4 G H2' H 4.527 0.02 . 38 4 4 G H3' H 4.590 0.02 . 39 4 4 G H4' H 4.511 0.02 . 40 4 4 G H5' H 4.530 0.02 . 41 4 4 G H5'' H 4.160 0.02 . 42 4 4 G H8 H 7.739 0.02 . 43 4 4 G C1' C 92.618 0.02 . 44 4 4 G C2' C 75.367 0.02 . 45 4 4 G C3' C 73.161 0.02 . 46 4 4 G C4' C 82.025 0.02 . 47 4 4 G C5' C 65.721 0.02 . 48 5 5 C H1' H 5.459 0.02 . 49 5 5 C H2' H 4.401 0.02 . 50 5 5 C H4' H 4.523 0.02 . 51 5 5 C H5 H 5.270 0.02 . 52 5 5 C H5'' H 4.085 0.02 . 53 5 5 C H6 H 7.656 0.02 . 54 5 5 C C1' C 93.895 0.02 . 55 6 6 C H1' H 5.460 0.02 . 56 6 6 C H2' H 4.352 0.02 . 57 6 6 C H5 H 5.514 0.02 . 58 6 6 C H5' H 4.436 0.02 . 59 6 6 C H5'' H 4.065 0.02 . 60 6 6 C H6 H 7.735 0.02 . 61 6 6 C C1' C 94.362 0.02 . 62 6 6 C C2' C 75.351 0.02 . 63 7 7 U H1' H 5.455 0.02 . 64 7 7 U H2' H 4.402 0.02 . 65 7 7 U H3' H 4.610 0.02 . 66 7 7 U H4' H 4.462 0.02 . 67 7 7 U H5 H 5.419 0.02 . 68 7 7 U H5' H 4.560 0.02 . 69 7 7 U H5'' H 4.094 0.02 . 70 7 7 U H6 H 7.848 0.02 . 71 7 7 U C1' C 93.728 0.02 . 72 7 7 U C2' C 75.449 0.02 . 73 7 7 U C3' C 72.282 0.02 . 74 8 8 A H1' H 5.865 0.02 . 75 8 8 A H2 H 6.651 0.02 . 76 8 8 A H2' H 4.420 0.02 . 77 8 8 A H3' H 4.681 0.02 . 78 8 8 A H4' H 4.467 0.02 . 79 8 8 A H5' H 4.522 0.02 . 80 8 8 A H5'' H 4.155 0.02 . 81 8 8 A H8 H 8.032 0.02 . 82 8 8 A C1' C 92.710 0.02 . 83 8 8 A C2' C 75.812 0.02 . 84 8 8 A C3' C 73.087 0.02 . 85 8 8 A C4' C 82.330 0.02 . 86 8 8 A C5' C 65.330 0.02 . 87 9 9 A H1' H 5.702 0.02 . 88 9 9 A H2 H 7.728 0.02 . 89 9 9 A H2' H 4.399 0.02 . 90 9 9 A H3' H 4.563 0.02 . 91 9 9 A H4' H 4.415 0.02 . 92 9 9 A H5' H 4.443 0.02 . 93 9 9 A H5'' H 4.081 0.02 . 94 9 9 A H8 H 7.683 0.02 . 95 9 9 A C1' C 91.713 0.02 . 96 9 9 A C2' C 76.290 0.02 . 97 9 9 A C3' C 74.431 0.02 . 98 9 9 A C4' C 83.189 0.02 . 99 9 9 A C5' C 65.974 0.02 . 100 10 10 U H1' H 5.528 0.02 . 101 10 10 U H2' H 4.206 0.02 . 102 10 10 U H3' H 4.400 0.02 . 103 10 10 U H4' H 4.214 0.02 . 104 10 10 U H5 H 5.528 0.02 . 105 10 10 U H5' H 4.085 0.02 . 106 10 10 U H5'' H 3.943 0.02 . 107 10 10 U H6 H 7.543 0.02 . 108 10 10 U C1' C 90.902 0.02 . 109 10 10 U C2' C 75.571 0.02 . 110 10 10 U C3' C 76.450 0.02 . 111 10 10 U C4' C 84.747 0.02 . 112 10 10 U C5' C 66.980 0.02 . 113 11 11 A H1' H 5.799 0.02 . 114 11 11 A H2 H 8.003 0.02 . 115 11 11 A H2' H 4.561 0.02 . 116 11 11 A H3' H 4.638 0.02 . 117 11 11 A H4' H 4.413 0.02 . 118 11 11 A H5' H 4.114 0.02 . 119 11 11 A H5'' H 4.000 0.02 . 120 11 11 A H8 H 8.011 0.02 . 121 11 11 A C1' C 90.218 0.02 . 122 11 11 A C2' C 77.184 0.02 . 123 11 11 A C3' C 77.921 0.02 . 124 11 11 A C4' C 86.000 0.02 . 125 11 11 A C5' C 67.831 0.02 . 126 12 12 U H1' H 5.920 0.02 . 127 12 12 U H2' H 4.380 0.02 . 128 12 12 U H3' H 4.684 0.02 . 129 12 12 U H4' H 4.454 0.02 . 130 12 12 U H5 H 5.772 0.02 . 131 12 12 U H5' H 4.153 0.02 . 132 12 12 U H5'' H 4.089 0.02 . 133 12 12 U H6 H 7.744 0.02 . 134 12 12 U C1' C 90.467 0.02 . 135 12 12 U C2' C 75.606 0.02 . 136 12 12 U C3' C 77.003 0.02 . 137 12 12 U C4' C 85.166 0.02 . 138 12 12 U C5' C 67.992 0.02 . 139 13 13 U H1' H 5.858 0.02 . 140 13 13 U H2' H 4.557 0.02 . 141 13 13 U H3' H 4.596 0.02 . 142 13 13 U H4' H 4.516 0.02 . 143 13 13 U H5 H 5.902 0.02 . 144 13 13 U H5' H 4.247 0.02 . 145 13 13 U H5'' H 4.201 0.02 . 146 13 13 U H6 H 7.903 0.02 . 147 13 13 U C1' C 91.160 0.02 . 148 13 13 U C2' C 74.889 0.02 . 149 13 13 U C3' C 76.425 0.02 . 150 13 13 U C4' C 84.607 0.02 . 151 13 13 U C5' C 68.415 0.02 . 152 14 14 U H1' H 5.698 0.02 . 153 14 14 U H2' H 4.592 0.02 . 154 14 14 U H3' H 4.553 0.02 . 155 14 14 U H4' H 4.556 0.02 . 156 14 14 U H5 H 5.802 0.02 . 157 14 14 U H5' H 4.418 0.02 . 158 14 14 U H5'' H 4.308 0.02 . 159 14 14 U H6 H 7.949 0.02 . 160 14 14 U C1' C 93.559 0.02 . 161 14 14 U C2' C 75.149 0.02 . 162 14 14 U C3' C 74.054 0.02 . 163 14 14 U C4' C 83.097 0.02 . 164 14 14 U C5' C 67.182 0.02 . 165 15 15 A H1' H 5.999 0.02 . 166 15 15 A H2 H 6.746 0.02 . 167 15 15 A H2' H 4.649 0.02 . 168 15 15 A H3' H 4.732 0.02 . 169 15 15 A H4' H 4.540 0.02 . 170 15 15 A H5' H 4.562 0.02 . 171 15 15 A H5'' H 4.246 0.02 . 172 15 15 A H8 H 8.208 0.02 . 173 15 15 A C1' C 92.478 0.02 . 174 15 15 A C2' C 75.731 0.02 . 175 15 15 A C3' C 73.135 0.02 . 176 15 15 A C4' C 82.138 0.02 . 177 15 15 A C5' C 65.690 0.02 . 178 16 16 G H1' H 5.534 0.02 . 179 16 16 G H2' H 4.476 0.02 . 180 16 16 G H3' H 4.422 0.02 . 181 16 16 G H4' H 4.436 0.02 . 182 16 16 G H5' H 4.447 0.02 . 183 16 16 G H5'' H 4.081 0.02 . 184 16 16 G H8 H 7.156 0.02 . 185 16 16 G C1' C 92.402 0.02 . 186 16 16 G C2' C 75.468 0.02 . 187 16 16 G C3' C 73.025 0.02 . 188 16 16 G C4' C 81.940 0.02 . 189 16 16 G C5' C 65.868 0.02 . 190 17 17 G H1' H 5.662 0.02 . 191 17 17 G H2' H 4.423 0.02 . 192 17 17 G H3' H 4.450 0.02 . 193 17 17 G H4' H 4.433 0.02 . 194 17 17 G H5' H 4.463 0.02 . 195 17 17 G H5'' H 4.033 0.02 . 196 17 17 G H8 H 7.154 0.02 . 197 17 17 G C1' C 92.934 0.02 . 198 17 17 G C2' C 75.319 0.02 . 199 17 17 G C3' C 72.759 0.02 . 200 17 17 G C4' C 81.964 0.02 . 201 17 17 G C5' C 65.270 0.02 . 202 18 18 C H1' H 5.484 0.02 . 203 18 18 C H2' H 4.527 0.02 . 204 18 18 C H3' H 4.515 0.02 . 205 18 18 C H4' H 4.411 0.02 . 206 18 18 C H5 H 5.212 0.02 . 207 18 18 C H5' H 4.549 0.02 . 208 18 18 C H5'' H 4.067 0.02 . 209 18 18 C H6 H 7.618 0.02 . 210 18 18 C C1' C 93.749 0.02 . 211 18 18 C C2' C 75.473 0.02 . 212 18 18 C C3' C 72.273 0.02 . 213 18 18 C C4' C 81.926 0.02 . 214 18 18 C C5' C 64.753 0.02 . 215 19 19 A H1' H 5.940 0.02 . 216 19 19 A H2 H 7.429 0.02 . 217 19 19 A H2' H 4.575 0.02 . 218 19 19 A H3' H 4.630 0.02 . 219 19 19 A H4' H 4.566 0.02 . 220 19 19 A H5' H 4.528 0.02 . 221 19 19 A H5'' H 4.133 0.02 . 222 19 19 A H8 H 8.038 0.02 . 223 19 19 A C1' C 93.195 0.02 . 224 19 19 A C2' C 75.559 0.02 . 225 19 19 A C3' C 72.926 0.02 . 226 19 19 A C4' C 82.521 0.02 . 227 19 19 A C5' C 65.233 0.02 . 228 20 20 C H1' H 5.406 0.02 . 229 20 20 C H2' H 4.068 0.02 . 230 20 20 C H3' H 4.356 0.02 . 231 20 20 C H4' H 4.360 0.02 . 232 20 20 C H5 H 5.218 0.02 . 233 20 20 C H5' H 4.507 0.02 . 234 20 20 C H5'' H 4.022 0.02 . 235 20 20 C H6 H 7.514 0.02 . 236 20 20 C C1' C 94.224 0.02 . 237 20 20 C C2' C 75.767 0.02 . 238 20 20 C C3' C 72.302 0.02 . 239 20 20 C C4' C 82.036 0.02 . 240 20 20 C C5' C 64.587 0.02 . 241 21 21 C H1' H 6.099 0.02 . 242 21 21 C H2' H 4.626 0.02 . 243 21 21 C H3' H 4.935 0.02 . 244 21 21 C H4' H 4.399 0.02 . 245 21 21 C H5 H 5.472 0.02 . 246 21 21 C H5' H 4.503 0.02 . 247 21 21 C H5'' H 4.075 0.02 . 248 21 21 C H6 H 7.457 0.02 . 249 21 21 C C1' C 91.985 0.02 . 250 21 21 C C2' C 85.173 0.02 . 251 21 21 C C3' C 77.977 0.02 . 252 21 21 C C4' C 85.572 0.02 . 253 21 21 C C5' C 65.759 0.02 . stop_ save_