data_4890
#######################
# Entry information #
#######################
save_entry_information
_Saveframe_category entry_information
_Entry_title
;
1H resonance assignments for the N-ter domain of the human TFIIH MAT1 subunit
;
_BMRB_accession_number 4890
_BMRB_flat_file_name bmr4890.str
_Entry_type original
_Submission_date 2000-11-02
_Accession_date 2000-11-02
_Entry_origination author
_NMR_STAR_version 2.1.1
_Experimental_method NMR
_Details .
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Gervais V. . .
2 Wasielewski E. . .
3 Busso D. . .
4 Poterszman A. . .
5 Egly J.M. . .
6 Thierry J.C. . .
7 Kieffer B. . .
stop_
loop_
_Saveframe_category_type
_Saveframe_category_type_count
assigned_chemical_shifts 1
stop_
loop_
_Data_type
_Data_type_count
"1H chemical shifts" 366
stop_
loop_
_Revision_date
_Revision_keyword
_Revision_author
_Revision_detail
2000-12-04 original author .
stop_
_Original_release_date 2000-12-04
save_
#############################
# Citation for this entry #
#############################
save_entry_citation
_Saveframe_category entry_citation
_Citation_full .
_Citation_title
;
Solution Structure of the N-terminal Domain of the Human TFIIH MAT1 Subunit: New
Insights into the RING Finger Family
;
_Citation_status 'in press'
_Citation_type journal
_CAS_abstract_code .
_MEDLINE_UI_code .
_PubMed_ID ?
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Gervais V. . .
2 Busso D. . .
3 Wasielewski E. . .
4 Poterszman A. . .
5 Egly J.M. . .
6 Thierry J.C. . .
7 Kieffer B. . .
stop_
_Journal_abbreviation 'J. Biol. Chem.'
_Journal_volume .
_Journal_issue .
_Journal_CSD .
_Book_chapter_title .
_Book_volume .
_Book_series .
_Book_ISBN .
_Conference_state_province .
_Conference_abstract_number .
_Page_first .
_Page_last .
_Year .
_Details .
loop_
_Keyword
'RING FINGER (C3HC4)'
'basic transcription factor'
'zinc binding protein'
stop_
save_
#######################################
# Cited references within the entry #
#######################################
save_ref_1
_Saveframe_category citation
_Citation_full
;
Yee A, Nichols MA, Wu L, Hall FL, Kobayashi R, Xiong Y.
Molecular cloning of CDK7-associated human MAT1, a cyclin-dependent
kinase-activating kinase (CAK) assembly factor.
Cancer Res. 1995 Dec 15;55(24):6058-62.
;
_Citation_title 'Molecular cloning of CDK7-associated human MAT1, a cyclin-dependent kinase-activating kinase (CAK) assembly factor.'
_Citation_status published
_Citation_type journal
_CAS_abstract_code .
_MEDLINE_UI_code .
_PubMed_ID 8521393
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Yee A. . .
2 Nichols 'M. A.' A. .
3 Wu L. . .
4 Hall 'F. L.' L. .
5 Kobayashi R. . .
6 Xiong Y. . .
stop_
_Journal_abbreviation 'Cancer Res.'
_Journal_name_full 'Cancer research'
_Journal_volume 55
_Journal_issue 24
_Journal_CSD .
_Book_title .
_Book_chapter_title .
_Book_volume .
_Book_series .
_Book_publisher .
_Book_publisher_city .
_Book_ISBN .
_Conference_title .
_Conference_site .
_Conference_state_province .
_Conference_country .
_Conference_start_date .
_Conference_end_date .
_Conference_abstract_number .
_Thesis_institution .
_Thesis_institution_city .
_Thesis_institution_country .
_Page_first 6058
_Page_last 6062
_Year 1995
_Details
;
Mammalian CDK7 is a protein kinase identified as the catalytic subunit of
cyclin-dependent kinase (CDK)-activating kinase and as an essential component
of the transcription factor TFIIH that is involved in transcription initiation
and DNA repair. We have identified in human cells a number of CDK7-associated
cellular proteins that appear to fall into two classes based on their relative
[35S] metabolic labeling intensity. One class of proteins present in CDK7
immunocomplexes as a minor fraction contains components of the TFIIH
transcription complex such as p62 and p89ERCC3, whereas the other fraction
contains four polypeptides (p35, p37Cyclin H, p75, and p95) that are
stoichiometrically associated with CDK7. Whereas the levels of association of
p35, p37Cyclin H, and p75 with CDK7 remain unchanged between density-arrested
and proliferating Ewing sarcoma EW-1 cells, the association of p95 with CDK7
was significantly decreased as cells reached confluency. Through a large-scale
immunopurification of CDK7 complexes and protein microsequencing, we have
isolated a cDNA that encodes p35 and have shown that it is the human homologue
of Mat1 that is involved in the assembly of CAK. MAT1 contains a highly
conserved C3HC4 motif at its NH2 terminus, a characteristic feature shared
among RING finger proteins. The human MAT1 gene expresses a single 1.6-kb
transcript, the steady-state level of which, like CDK7 and cyclin H, varies
significantly in different cell lines and in different terminally
differentiated tissues.
;
save_
save_ref_2
_Saveframe_category citation
_Citation_full .
_Citation_title 'X-PLOR, version 3.1: A system for X-ray crystallography and NMR'
_Citation_status .
_Citation_type book
_CAS_abstract_code .
_MEDLINE_UI_code .
_PubMed_ID ?
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Brunger Axel T. .
stop_
_Journal_abbreviation .
_Journal_name_full .
_Journal_volume .
_Journal_issue .
_Journal_CSD .
_Book_title 'X-PLOR, version 3.1: A system for X-ray crystallography and NMR'
_Book_chapter_title .
_Book_volume .
_Book_series .
_Book_publisher 'Yale University Press'
_Book_publisher_city 'New Haven'
_Book_ISBN 0300054025
_Conference_title .
_Conference_site .
_Conference_state_province .
_Conference_country .
_Conference_start_date .
_Conference_end_date .
_Conference_abstract_number .
_Thesis_institution .
_Thesis_institution_city .
_Thesis_institution_country .
_Page_first .
_Page_last .
_Year 1992
_Details .
loop_
_Keyword
'structure calculation'
'structure analysis'
software
stop_
save_
save_ref_3
_Saveframe_category citation
_Citation_full .
_Citation_title
;
The program XEASY for computer-supported NMR spectral analysis of biological
macromolecules.
;
_Citation_status .
_Citation_type journal
_CAS_abstract_code .
_MEDLINE_UI_code .
_PubMed_ID ?
loop_
_Author_ordinal
_Author_family_name
_Author_given_name
_Author_middle_initials
_Author_family_title
1 Bartels C. . .
2 Xia T.-H. . .
3 Billeter Martin . .
4 Guntert Peter . .
5 Wuthrich Kurt . .
stop_
_Journal_abbreviation 'J. Biomol. NMR'
_Journal_name_full .
_Journal_volume 6
_Journal_issue .
_Journal_CSD .
_Book_title .
_Book_chapter_title .
_Book_volume .
_Book_series .
_Book_publisher .
_Book_publisher_city .
_Book_ISBN .
_Conference_title .
_Conference_site .
_Conference_state_province .
_Conference_country .
_Conference_start_date .
_Conference_end_date .
_Conference_abstract_number .
_Thesis_institution .
_Thesis_institution_city .
_Thesis_institution_country .
_Page_first 1
_Page_last 10
_Year 1995
_Details .
loop_
_Keyword
'spectrum analysis'
software
stop_
save_
##################################
# Molecular system description #
##################################
save_system_MAT1
_Saveframe_category molecular_system
_Mol_system_name 'CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1'
_Abbreviation_common MAT1
_Enzyme_commission_number .
loop_
_Mol_system_component_name
_Mol_label
MAT1 $MAT1
Zn(II)-1 $ZN
Zn(II)-2 $ZN
stop_
_System_molecular_weight .
_System_physical_state native
_System_oligomer_state monomer
_System_paramagnetic no
_System_thiol_state 'all other bound'
_Database_query_date .
_Details .
save_
########################
# Monomeric polymers #
########################
save_MAT1
_Saveframe_category monomeric_polymer
_Mol_type polymer
_Mol_polymer_class protein
_Name_common 'Menage a trois'
_Abbreviation_common MAT1
_Molecular_mass 7296
_Mol_thiol_state 'not reported'
_Details .
##############################
# Polymer residue sequence #
##############################
_Residue_count 65
_Mol_residue_sequence
;
MDDQGCPRCKTTKYRNPSLK
LMVNVCGHTLCESCVDLLFV
RGAGNCPECGTPLRKSNFRV
QLFED
;
loop_
_Residue_seq_code
_Residue_label
1 MET 2 ASP 3 ASP 4 GLN 5 GLY
6 CYS 7 PRO 8 ARG 9 CYS 10 LYS
11 THR 12 THR 13 LYS 14 TYR 15 ARG
16 ASN 17 PRO 18 SER 19 LEU 20 LYS
21 LEU 22 MET 23 VAL 24 ASN 25 VAL
26 CYS 27 GLY 28 HIS 29 THR 30 LEU
31 CYS 32 GLU 33 SER 34 CYS 35 VAL
36 ASP 37 LEU 38 LEU 39 PHE 40 VAL
41 ARG 42 GLY 43 ALA 44 GLY 45 ASN
46 CYS 47 PRO 48 GLU 49 CYS 50 GLY
51 THR 52 PRO 53 LEU 54 ARG 55 LYS
56 SER 57 ASN 58 PHE 59 ARG 60 VAL
61 GLN 62 LEU 63 PHE 64 GLU 65 ASP
stop_
_Sequence_homology_query_date 2008-08-19
_Sequence_homology_query_revised_last_date 2008-08-19
loop_
_Database_name
_Database_accession_code
_Database_entry_mol_name
_Sequence_query_to_submitted_percentage
_Sequence_subject_length
_Sequence_identity
_Sequence_positive
_Sequence_homology_expectation_value
SWISS-PROT P51948 'CDK-activating kinase assembly factor MAT1 (RING finger protein MAT1) (Menage a trois) (CDK7/cyclin H assembly factor) (p36) (p35) (Cyclin G1-interacting protein) (RING finger protein 66)' 100.00 309 100.00 100.00 5.19e-33
SWISS-PROT P51949 'CDK-activating kinase assembly factor MAT1 (RING finger protein MAT1) (Menage a trois) (CDK7/cyclin H assembly factor) (p36) (p35)' 100.00 309 100.00 100.00 4.66e-33
REF NP_703202 'menage a trois 1 [Rattus norvegicus]' 100.00 309 100.00 100.00 4.39e-33
REF XP_001096910 'PREDICTED: menage a trois 1 (CAK assembly factor) isoform 1 [Macaca mulatta]' 100.00 278 100.00 100.00 4.50e-33
REF NP_002422 'menage a trois 1 (CAK assembly factor) [Homo sapiens]' 100.00 309 100.00 100.00 5.19e-33
REF NP_032638 'menage a trois 1 [Mus musculus]' 100.00 309 100.00 100.00 4.66e-33
GenBank AAH89023 'Menage a trois 1 [Mus musculus]' 100.00 309 100.00 100.00 4.66e-33
REF NP_001098879 'menage a trois homolog 1, cyclin H assembly factor [Bos taurus]' 100.00 309 100.00 100.00 6.03e-33
GenBank AAH00820 'Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) [Homo sapiens]' 100.00 309 100.00 100.00 5.19e-33
GenBank AAH78712 'Menage a trois 1 [Rattus norvegicus]' 100.00 309 100.00 100.00 4.39e-33
GenBank AAA91741 'CDK-activating kinase assembly factor p36/MAT1' 100.00 309 100.00 100.00 4.62e-33
GenBank AAB05248 '1500GX1 gene product' 100.00 267 98.46 98.46 4.86e-32
EMBL CAA63356 'p35 [Homo sapiens]' 100.00 309 100.00 100.00 5.19e-33
EMBL CAG29332 'MNAT1 [Homo sapiens]' 100.00 309 100.00 100.00 5.19e-33
DBJ BAE34168 'unnamed protein product [Mus musculus]' 100.00 309 100.00 100.00 4.66e-33
EMBL CAA61112 'cdk7/cyclin H assembly factor [Homo sapiens]' 100.00 309 100.00 100.00 5.19e-33
DBJ BAB25556 'unnamed protein product [Mus musculus]' 100.00 309 100.00 100.00 4.66e-33
DBJ BAE33464 'unnamed protein product [Mus musculus]' 100.00 309 100.00 100.00 4.66e-33
PDB 1G25 'Solution Structure Of The N-Terminal Domain Of The Human Tfiih Mat1 Subunit' 100.00 65 100.00 100.00 1.12e-30
DBJ BAB25483 'unnamed protein product [Mus musculus]' 100.00 309 98.46 98.46 5.19e-32
stop_
save_
#############
# Ligands #
#############
save_ZN
_Saveframe_category ligand
_Mol_type non-polymer
_Name_common "ZN (ZINC ION)"
_BMRB_code .
_PDB_code ZN
_Molecular_mass 65.409
_Mol_charge 2
_Mol_paramagnetic .
_Mol_aromatic no
_Details
;
Information obtained from PDB's Chemical Component Dictionary
at http://wwpdb-remediation.rutgers.edu/downloads.html
Downloaded on Thu Jul 21 14:17:05 2011
;
loop_
_Atom_name
_PDB_atom_name
_Atom_type
_Atom_chirality
_Atom_charge
_Atom_oxidation_number
_Atom_unpaired_electrons
ZN ZN ZN . 2 . ?
stop_
_Mol_thiol_state .
_Sequence_homology_query_date .
save_
####################
# Natural source #
####################
save_natural_source
_Saveframe_category natural_source
loop_
_Mol_label
_Organism_name_common
_NCBI_taxonomy_ID
_Superkingdom
_Kingdom
_Genus
_Species
_Gene_mnemonic
$MAT1 human 9606 Eukaryota Metazoa Homo sapiens MAT1
stop_
save_
#########################
# Experimental source #
#########################
save_experimental_source
_Saveframe_category experimental_source
loop_
_Mol_label
_Production_method
_Host_organism_name_common
_Genus
_Species
_Strain
_Vector_type
_Vector_name
$MAT1 'recombinant technology' 'E. coli' Escherichia coli BL21 plasmid pGEX-4T2
stop_
save_
#####################################
# Sample contents and methodology #
#####################################
########################
# Sample description #
########################
save_sample_1
_Saveframe_category sample
_Sample_type solution
_Details .
loop_
_Mol_label
_Concentration_value
_Concentration_value_units
_Isotopic_labeling
$MAT1 2 mM .
stop_
save_
############################
# Computer software used #
############################
save_X-PLOR
_Saveframe_category software
_Name X-PLOR
_Version 3.8
loop_
_Task
'structure solution'
refinement
stop_
_Details .
_Citation_label $ref_2
save_
save_XWINNMR
_Saveframe_category software
_Name XWINNMR
_Version 1.3
loop_
_Task
'data collection'
'data processing'
stop_
_Details Bruker
save_
save_xeasy
_Saveframe_category software
_Name xeasy
_Version 1.3.13
loop_
_Task
'data analysis'
stop_
_Details .
_Citation_label $ref_3
save_
#########################
# Experimental detail #
#########################
##################################
# NMR Spectrometer definitions #
##################################
save_spectrometer_1
_Saveframe_category NMR_spectrometer
_Manufacturer Bruker
_Model DMX
_Field_strength 750
_Details .
save_
#############################
# NMR applied experiments #
#############################
save_2D_NOESY_1
_Saveframe_category NMR_applied_experiment
_Experiment_name '2D NOESY'
_Sample_label $sample_1
save_
save_DQF-COSY_2
_Saveframe_category NMR_applied_experiment
_Experiment_name DQF-COSY
_Sample_label $sample_1
save_
save_2D_TOCSY_3
_Saveframe_category NMR_applied_experiment
_Experiment_name '2D TOCSY'
_Sample_label $sample_1
save_
#######################
# Sample conditions #
#######################
save_MAT1_conditions
_Saveframe_category sample_conditions
_Details .
loop_
_Variable_type
_Variable_value
_Variable_value_error
_Variable_value_units
pH 7.5 0.1 n/a
temperature 298 0.1 K
stop_
save_
####################
# NMR parameters #
####################
##############################
# Assigned chemical shifts #
##############################
################################
# Chemical shift referencing #
################################
save_chemical_shift_reference
_Saveframe_category chemical_shift_reference
_Details .
loop_
_Mol_common_name
_Atom_type
_Atom_isotope_number
_Atom_group
_Chem_shift_units
_Chem_shift_value
_Reference_method
_Reference_type
_External_reference_sample_geometry
_External_reference_location
_External_reference_axis
_Indirect_shift_ratio
DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0
stop_
save_
###################################
# Assigned chemical shift lists #
###################################
###################################################################
# Chemical Shift Ambiguity Index Value Definitions #
# #
# The values other than 1 are used for those atoms with different #
# chemical shifts that cannot be assigned to stereospecific atoms #
# or to specific residues or chains. #
# #
# Index Value Definition #
# #
# 1 Unique (including isolated methyl protons, #
# geminal atoms, and geminal methyl #
# groups with identical chemical shifts) #
# (e.g. ILE HD11, HD12, HD13 protons) #
# 2 Ambiguity of geminal atoms or geminal methyl #
# proton groups (e.g. ASP HB2 and HB3 #
# protons, LEU CD1 and CD2 carbons, or #
# LEU HD11, HD12, HD13 and HD21, HD22, #
# HD23 methyl protons) #
# 3 Aromatic atoms on opposite sides of #
# symmetrical rings (e.g. TYR HE1 and HE2 #
# protons) #
# 4 Intraresidue ambiguities (e.g. LYS HG and #
# HD protons or TRP HZ2 and HZ3 protons) #
# 5 Interresidue ambiguities (LYS 12 vs. LYS 27) #
# 6 Intermolecular ambiguities (e.g. ASP 31 CA #
# in monomer 1 and ASP 31 CA in monomer 2 #
# of an asymmetrical homodimer, duplex #
# DNA assignments, or other assignments #
# that may apply to atoms in one or more #
# molecule in the molecular assembly) #
# 9 Ambiguous, specific ambiguity not defined #
# #
###################################################################
save_chemical_shift_set_1
_Saveframe_category assigned_chemical_shifts
_Details .
loop_
_Sample_label
$sample_1
stop_
_Sample_conditions_label $MAT1_conditions
_Chem_shift_reference_set_label $chemical_shift_reference
_Mol_system_component_name MAT1
_Text_data_format .
_Text_data .
loop_
_Atom_shift_assign_ID
_Residue_author_seq_code
_Residue_seq_code
_Residue_label
_Atom_name
_Atom_type
_Chem_shift_value
_Chem_shift_value_error
_Chem_shift_ambiguity_code
1 . 2 ASP H H 8.04 0.02 1
2 . 2 ASP HA H 4.91 0.02 1
3 . 2 ASP HB2 H 4.58 0.02 1
4 . 2 ASP HB3 H 4.58 0.02 1
5 . 3 ASP HA H 4.58 0.02 1
6 . 3 ASP HB2 H 2.66 0.02 1
7 . 3 ASP HB3 H 2.66 0.02 1
8 . 4 GLN H H 7.99 0.02 1
9 . 4 GLN HA H 4.25 0.02 1
10 . 4 GLN HB2 H 1.92 0.02 1
11 . 4 GLN HB3 H 2.00 0.02 1
12 . 4 GLN HG2 H 2.23 0.02 2
13 . 4 GLN HG3 H 2.17 0.02 2
14 . 4 GLN HE21 H 7.46 0.02 1
15 . 4 GLN HE22 H 6.80 0.02 1
16 . 5 GLY H H 8.04 0.02 1
17 . 5 GLY HA2 H 4.26 0.02 2
18 . 5 GLY HA3 H 3.90 0.02 2
19 . 6 CYS H H 8.32 0.02 1
20 . 6 CYS HA H 4.66 0.02 1
21 . 6 CYS HB2 H 2.57 0.02 1
22 . 6 CYS HB3 H 3.53 0.02 1
23 . 7 PRO HA H 4.43 0.02 1
24 . 7 PRO HB2 H 2.42 0.02 2
25 . 7 PRO HB3 H 2.10 0.02 2
26 . 7 PRO HG2 H 2.35 0.02 2
27 . 7 PRO HG3 H 2.20 0.02 2
28 . 7 PRO HD2 H 4.15 0.02 2
29 . 7 PRO HD3 H 4.10 0.02 2
30 . 8 ARG H H 8.60 0.02 1
31 . 8 ARG HA H 4.42 0.02 1
32 . 8 ARG HB2 H 1.72 0.02 1
33 . 8 ARG HB3 H 2.39 0.02 1
34 . 8 ARG HG2 H 1.63 0.02 2
35 . 8 ARG HG3 H 1.54 0.02 2
36 . 8 ARG HD2 H 3.45 0.02 2
37 . 8 ARG HD3 H 3.08 0.02 2
38 . 9 CYS H H 8.16 0.02 1
39 . 9 CYS HA H 4.73 0.02 1
40 . 9 CYS HB2 H 3.28 0.02 1
41 . 9 CYS HB3 H 2.51 0.02 1
42 . 10 LYS H H 7.77 0.02 1
43 . 10 LYS HA H 4.18 0.02 1
44 . 10 LYS HB2 H 2.28 0.02 1
45 . 10 LYS HB3 H 2.04 0.02 1
46 . 10 LYS HG2 H 1.38 0.02 2
47 . 10 LYS HG3 H 1.21 0.02 2
48 . 10 LYS HD2 H 1.57 0.02 1
49 . 10 LYS HD3 H 1.57 0.02 1
50 . 10 LYS HE2 H 3.03 0.02 1
51 . 10 LYS HE3 H 3.03 0.02 1
52 . 11 THR H H 8.17 0.02 1
53 . 11 THR HA H 4.24 0.02 1
54 . 11 THR HB H 4.08 0.02 1
55 . 11 THR HG2 H 1.42 0.02 1
56 . 12 THR H H 8.17 0.02 1
57 . 12 THR HA H 4.24 0.02 1
58 . 12 THR HB H 4.09 0.02 1
59 . 12 THR HG2 H 1.42 0.02 1
60 . 13 LYS H H 8.89 0.02 1
61 . 13 LYS HA H 4.32 0.02 1
62 . 13 LYS HB2 H 1.35 0.02 1
63 . 13 LYS HB3 H 1.29 0.02 1
64 . 13 LYS HG2 H 0.75 0.02 1
65 . 13 LYS HD2 H 1.23 0.02 1
66 . 13 LYS HD3 H 1.23 0.02 1
67 . 13 LYS HE2 H 2.91 0.02 1
68 . 13 LYS HE3 H 2.91 0.02 1
69 . 14 TYR H H 7.70 0.02 1
70 . 14 TYR HA H 4.24 0.02 1
71 . 14 TYR HB2 H 3.00 0.02 2
72 . 14 TYR HB3 H 2.96 0.02 2
73 . 14 TYR HD1 H 7.12 0.02 1
74 . 14 TYR HD2 H 7.12 0.02 1
75 . 14 TYR HE1 H 6.86 0.02 1
76 . 14 TYR HE2 H 6.86 0.02 1
77 . 15 ARG H H 7.35 0.02 1
78 . 15 ARG HA H 4.20 0.02 1
79 . 15 ARG HB2 H 2.01 0.02 1
80 . 15 ARG HB3 H 1.87 0.02 1
81 . 15 ARG HG2 H 1.71 0.02 1
82 . 15 ARG HG3 H 1.71 0.02 1
83 . 15 ARG HD2 H 3.29 0.02 1
84 . 15 ARG HD3 H 3.29 0.02 1
85 . 15 ARG HE H 7.43 0.02 1
86 . 16 ASN H H 8.01 0.02 1
87 . 16 ASN HA H 5.12 0.02 1
88 . 16 ASN HB2 H 2.71 0.02 2
89 . 16 ASN HB3 H 2.86 0.02 2
90 . 16 ASN HD21 H 7.19 0.02 1
91 . 16 ASN HD22 H 7.10 0.02 1
92 . 17 PRO HA H 4.62 0.02 1
93 . 17 PRO HB2 H 2.45 0.02 1
94 . 17 PRO HB3 H 2.45 0.02 1
95 . 17 PRO HG2 H 2.06 0.02 2
96 . 17 PRO HG3 H 2.04 0.02 2
97 . 17 PRO HD2 H 3.85 0.02 2
98 . 17 PRO HD3 H 3.50 0.02 2
99 . 18 SER H H 7.68 0.02 1
100 . 18 SER HA H 4.54 0.02 1
101 . 18 SER HB2 H 3.97 0.02 1
102 . 18 SER HB3 H 3.97 0.02 1
103 . 19 LEU H H 7.60 0.02 1
104 . 19 LEU HA H 3.97 0.02 1
105 . 19 LEU HB2 H 1.71 0.02 1
106 . 19 LEU HB3 H 1.71 0.02 1
107 . 19 LEU HG H 1.35 0.02 1
108 . 19 LEU HD1 H 0.94 0.02 2
109 . 19 LEU HD2 H 0.74 0.02 2
110 . 20 LYS H H 8.83 0.02 1
111 . 20 LYS HA H 4.57 0.02 1
112 . 20 LYS HB2 H 1.74 0.02 1
113 . 20 LYS HB3 H 1.74 0.02 1
114 . 20 LYS HG2 H 1.33 0.02 1
115 . 20 LYS HG3 H 1.33 0.02 1
116 . 20 LYS HD2 H 1.52 0.02 1
117 . 20 LYS HD3 H 1.52 0.02 1
118 . 20 LYS HE2 H 2.99 0.02 1
119 . 20 LYS HE3 H 2.99 0.02 1
120 . 21 LEU H H 8.65 0.02 1
121 . 21 LEU HA H 4.47 0.02 1
122 . 21 LEU HB2 H 1.56 0.02 1
123 . 21 LEU HB3 H 1.90 0.02 1
124 . 21 LEU HG H 1.25 0.02 1
125 . 21 LEU HD1 H 0.84 0.02 2
126 . 21 LEU HD2 H 0.66 0.02 2
127 . 22 MET H H 8.56 0.02 1
128 . 22 MET HA H 5.37 0.02 1
129 . 22 MET HB2 H 2.00 0.02 2
130 . 22 MET HB3 H 1.95 0.02 2
131 . 22 MET HG2 H 2.36 0.02 2
132 . 22 MET HG3 H 2.18 0.02 2
133 . 23 VAL H H 9.32 0.02 1
134 . 23 VAL HA H 4.93 0.02 1
135 . 23 VAL HB H 1.93 0.02 1
136 . 23 VAL HG1 H 0.99 0.02 2
137 . 23 VAL HG2 H 0.82 0.02 2
138 . 24 ASN H H 7.78 0.02 1
139 . 24 ASN HA H 4.99 0.02 1
140 . 24 ASN HB2 H 3.04 0.02 1
141 . 24 ASN HB3 H 3.04 0.02 1
142 . 24 ASN HD21 H 6.57 0.02 1
143 . 24 ASN HD22 H 6.57 0.02 1
144 . 25 VAL H H 9.87 0.02 1
145 . 25 VAL HA H 3.91 0.02 1
146 . 25 VAL HB H 2.20 0.02 1
147 . 25 VAL HG1 H 1.05 0.02 2
148 . 25 VAL HG2 H 0.93 0.02 2
149 . 26 CYS H H 7.04 0.02 1
150 . 26 CYS HA H 4.62 0.02 1
151 . 26 CYS HB2 H 3.25 0.02 1
152 . 26 CYS HB3 H 2.75 0.02 1
153 . 27 GLY H H 7.67 0.02 1
154 . 27 GLY HA2 H 3.50 0.02 2
155 . 27 GLY HA3 H 3.85 0.02 2
156 . 28 HIS H H 8.14 0.02 1
157 . 28 HIS HA H 5.07 0.02 1
158 . 28 HIS HB2 H 3.11 0.02 1
159 . 28 HIS HB3 H 3.40 0.02 1
160 . 28 HIS HD2 H 7.28 0.02 1
161 . 28 HIS HE1 H 7.88 0.02 1
162 . 29 THR H H 9.07 0.02 1
163 . 29 THR HA H 5.09 0.02 1
164 . 29 THR HB H 4.01 0.02 1
165 . 29 THR HG2 H 1.33 0.02 1
166 . 30 LEU H H 8.94 0.02 1
167 . 30 LEU HA H 5.03 0.02 1
168 . 30 LEU HB2 H 1.87 0.02 2
169 . 30 LEU HB3 H 1.48 0.02 2
170 . 30 LEU HG H 1.01 0.02 1
171 . 30 LEU HD1 H 0.66 0.02 2
172 . 30 LEU HD2 H 0.01 0.02 2
173 . 31 CYS H H 9.37 0.02 1
174 . 31 CYS HA H 5.43 0.02 1
175 . 31 CYS HB2 H 3.47 0.02 1
176 . 31 CYS HB3 H 2.79 0.02 1
177 . 32 GLU H H 8.20 0.02 1
178 . 32 GLU HA H 3.72 0.02 1
179 . 32 GLU HB2 H 2.13 0.02 2
180 . 32 GLU HB3 H 2.04 0.02 2
181 . 32 GLU HG2 H 2.27 0.02 1
182 . 32 GLU HG3 H 2.27 0.02 1
183 . 33 SER H H 7.97 0.02 1
184 . 33 SER HA H 4.37 0.02 1
185 . 33 SER HB2 H 4.09 0.02 1
186 . 33 SER HB3 H 4.05 0.02 1
187 . 34 CYS H H 8.96 0.02 1
188 . 34 CYS HA H 3.96 0.02 1
189 . 34 CYS HB2 H 2.82 0.02 1
190 . 34 CYS HB3 H 3.32 0.02 1
191 . 35 VAL H H 8.59 0.02 1
192 . 35 VAL HA H 3.34 0.02 1
193 . 35 VAL HB H 1.95 0.02 1
194 . 35 VAL HG1 H 0.39 0.02 2
195 . 35 VAL HG2 H 0.01 0.02 2
196 . 36 ASP H H 8.08 0.02 1
197 . 36 ASP HA H 4.30 0.02 1
198 . 36 ASP HB2 H 2.61 0.02 1
199 . 36 ASP HB3 H 2.74 0.02 1
200 . 37 LEU H H 7.66 0.02 1
201 . 37 LEU HA H 4.18 0.02 1
202 . 37 LEU HB2 H 2.28 0.02 2
203 . 37 LEU HB3 H 2.04 0.02 2
204 . 37 LEU HG H 1.56 0.02 1
205 . 37 LEU HD1 H 1.37 0.02 2
206 . 37 LEU HD2 H 1.20 0.02 2
207 . 38 LEU H H 8.20 0.02 1
208 . 38 LEU HA H 4.13 0.02 1
209 . 38 LEU HB2 H 1.79 0.02 1
210 . 38 LEU HB3 H 1.79 0.02 1
211 . 38 LEU HG H 1.20 0.02 1
212 . 38 LEU HD1 H 0.90 0.02 2
213 . 38 LEU HD2 H 0.84 0.02 2
214 . 39 PHE H H 8.21 0.02 1
215 . 39 PHE HA H 4.58 0.02 1
216 . 39 PHE HB2 H 3.02 0.02 1
217 . 39 PHE HB3 H 3.17 0.02 1
218 . 39 PHE HD1 H 7.34 0.02 1
219 . 39 PHE HD2 H 7.34 0.02 1
220 . 39 PHE HE1 H 7.14 0.02 1
221 . 39 PHE HE2 H 7.14 0.02 1
222 . 39 PHE HZ H 7.08 0.02 1
223 . 40 VAL H H 7.34 0.02 1
224 . 40 VAL HA H 4.11 0.02 1
225 . 40 VAL HB H 2.27 0.02 1
226 . 40 VAL HG1 H 1.09 0.02 2
227 . 40 VAL HG2 H 1.04 0.02 2
228 . 41 ARG H H 8.71 0.02 1
229 . 41 ARG HA H 4.45 0.02 1
230 . 41 ARG HB2 H 2.18 0.02 1
231 . 41 ARG HB3 H 1.92 0.02 1
232 . 41 ARG HG2 H 1.76 0.02 1
233 . 41 ARG HG3 H 1.72 0.02 1
234 . 41 ARG HD2 H 3.29 0.02 1
235 . 41 ARG HD3 H 3.25 0.02 1
236 . 42 GLY H H 8.37 0.02 1
237 . 42 GLY HA2 H 4.53 0.02 2
238 . 42 GLY HA3 H 3.75 0.02 2
239 . 43 ALA H H 7.72 0.02 1
240 . 43 ALA HA H 5.23 0.02 1
241 . 43 ALA HB H 1.53 0.02 1
242 . 44 GLY H H 8.29 0.02 1
243 . 44 GLY HA2 H 3.90 0.02 2
244 . 44 GLY HA3 H 3.37 0.02 2
245 . 45 ASN H H 8.49 0.02 1
246 . 45 ASN HA H 5.28 0.02 1
247 . 45 ASN HB2 H 2.24 0.02 1
248 . 45 ASN HB3 H 2.41 0.02 1
249 . 45 ASN HD21 H 7.35 0.02 1
250 . 45 ASN HD22 H 6.83 0.02 1
251 . 46 CYS H H 9.18 0.02 1
252 . 46 CYS HA H 4.53 0.02 1
253 . 46 CYS HB2 H 3.20 0.02 1
254 . 46 CYS HB3 H 3.31 0.02 1
255 . 47 PRO HA H 4.45 0.02 1
256 . 47 PRO HB2 H 2.38 0.02 2
257 . 47 PRO HB3 H 2.20 0.02 2
258 . 47 PRO HG2 H 2.05 0.02 1
259 . 47 PRO HG3 H 2.05 0.02 1
260 . 47 PRO HD2 H 4.52 0.02 2
261 . 47 PRO HD3 H 4.02 0.02 2
262 . 48 GLU H H 8.87 0.02 1
263 . 48 GLU HA H 4.30 0.02 1
264 . 48 GLU HB2 H 1.54 0.02 1
265 . 48 GLU HB3 H 1.37 0.02 1
266 . 48 GLU HG2 H 1.82 0.02 2
267 . 48 GLU HG3 H 1.65 0.02 2
268 . 49 CYS H H 7.97 0.02 1
269 . 49 CYS HA H 5.11 0.02 1
270 . 49 CYS HB2 H 3.53 0.02 2
271 . 49 CYS HB3 H 2.68 0.02 2
272 . 50 GLY H H 8.36 0.02 1
273 . 50 GLY HA2 H 4.30 0.02 2
274 . 50 GLY HA3 H 3.77 0.02 2
275 . 51 THR H H 8.24 0.02 1
276 . 51 THR HA H 4.38 0.02 1
277 . 51 THR HB H 4.21 0.02 1
278 . 51 THR HG2 H 1.25 0.02 1
279 . 52 PRO HA H 4.82 0.02 1
280 . 52 PRO HB2 H 2.36 0.02 2
281 . 52 PRO HB3 H 2.00 0.02 2
282 . 52 PRO HG2 H 2.19 0.02 2
283 . 52 PRO HG3 H 2.02 0.02 2
284 . 52 PRO HD2 H 4.09 0.02 1
285 . 52 PRO HD3 H 3.79 0.02 1
286 . 53 LEU H H 9.02 0.02 1
287 . 53 LEU HA H 4.82 0.02 1
288 . 53 LEU HB2 H 2.07 0.02 2
289 . 53 LEU HB3 H 1.81 0.02 2
290 . 53 LEU HG H 1.25 0.02 1
291 . 53 LEU HD1 H 0.94 0.02 2
292 . 53 LEU HD2 H 0.81 0.02 2
293 . 54 ARG H H 9.18 0.02 1
294 . 54 ARG HA H 4.52 0.02 1
295 . 54 ARG HD2 H 3.75 0.02 1
296 . 54 ARG HD3 H 3.75 0.02 1
297 . 55 LYS H H 8.87 0.02 1
298 . 55 LYS HA H 4.97 0.02 1
299 . 55 LYS HB2 H 2.05 0.02 1
300 . 55 LYS HB3 H 2.05 0.02 1
301 . 55 LYS HG2 H 1.73 0.02 1
302 . 55 LYS HG3 H 1.73 0.02 1
303 . 55 LYS HD2 H 1.80 0.02 1
304 . 55 LYS HD3 H 1.80 0.02 1
305 . 55 LYS HE2 H 3.22 0.02 1
306 . 55 LYS HE3 H 3.22 0.02 1
307 . 56 SER H H 8.84 0.02 1
308 . 56 SER HA H 4.97 0.02 1
309 . 56 SER HB2 H 3.71 0.02 1
310 . 56 SER HB3 H 3.71 0.02 1
311 . 57 ASN H H 7.20 0.02 1
312 . 57 ASN HA H 4.96 0.02 1
313 . 57 ASN HB2 H 2.99 0.02 1
314 . 57 ASN HB3 H 2.70 0.02 1
315 . 57 ASN HD21 H 7.83 0.02 1
316 . 57 ASN HD22 H 6.36 0.02 1
317 . 58 PHE H H 7.59 0.02 1
318 . 58 PHE HA H 4.61 0.02 1
319 . 58 PHE HB2 H 3.07 0.02 1
320 . 58 PHE HB3 H 2.97 0.02 1
321 . 58 PHE HD1 H 7.29 0.02 1
322 . 58 PHE HD2 H 7.29 0.02 1
323 . 58 PHE HE1 H 6.90 0.02 1
324 . 58 PHE HE2 H 6.90 0.02 1
325 . 58 PHE HZ H 6.90 0.02 1
326 . 59 ARG H H 9.12 0.02 1
327 . 59 ARG HA H 4.81 0.02 1
328 . 59 ARG HB2 H 1.80 0.02 1
329 . 59 ARG HB3 H 1.76 0.02 1
330 . 59 ARG HG2 H 1.64 0.02 2
331 . 59 ARG HG3 H 1.51 0.02 2
332 . 59 ARG HD2 H 3.18 0.02 2
333 . 59 ARG HD3 H 3.15 0.02 2
334 . 60 VAL H H 8.61 0.02 1
335 . 60 VAL HA H 4.20 0.02 1
336 . 60 VAL HB H 1.99 0.02 1
337 . 60 VAL HG1 H 1.04 0.02 2
338 . 60 VAL HG2 H 0.97 0.02 2
339 . 61 GLN H H 8.81 0.02 1
340 . 61 GLN HA H 4.25 0.02 1
341 . 61 GLN HB2 H 1.86 0.02 1
342 . 61 GLN HB3 H 1.60 0.02 1
343 . 61 GLN HG2 H 1.93 0.02 1
344 . 61 GLN HG3 H 1.93 0.02 1
345 . 62 LEU H H 8.26 0.02 1
346 . 62 LEU HA H 4.42 0.02 1
347 . 62 LEU HB2 H 1.58 0.02 1
348 . 62 LEU HB3 H 1.49 0.02 1
349 . 62 LEU HD1 H 0.92 0.02 2
350 . 62 LEU HD2 H 0.85 0.02 2
351 . 63 PHE H H 8.50 0.02 1
352 . 63 PHE HA H 4.63 0.02 1
353 . 63 PHE HB2 H 2.95 0.02 1
354 . 63 PHE HB3 H 3.21 0.02 1
355 . 63 PHE HD1 H 7.29 0.02 1
356 . 63 PHE HD2 H 7.29 0.02 1
357 . 64 GLU H H 8.47 0.02 1
358 . 64 GLU HA H 4.38 0.02 1
359 . 64 GLU HB2 H 1.93 0.02 2
360 . 64 GLU HB3 H 1.90 0.02 2
361 . 64 GLU HG2 H 2.24 0.02 2
362 . 64 GLU HG3 H 2.11 0.02 2
363 . 65 ASP H H 8.02 0.02 1
364 . 65 ASP HA H 4.38 0.02 1
365 . 65 ASP HB2 H 2.70 0.02 2
366 . 65 ASP HB3 H 2.58 0.02 2
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