data_1996 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR Study of the Phosphate-Binding Elements of Escherichia coli Elongation Factor Tu Catalytic Domain ; _BMRB_accession_number 1996 _BMRB_flat_file_name bmr1996.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lowry David F. . 2 Cool Robert H. . 3 Redfield Alfred G. . 4 Parmeggiani Andrea . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 4 "15N chemical shifts" 4 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-16 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Lowry, David F., Cool, Robert H., Redfield, Alfred G., Parmeggiani, Andrea, "NMR Study of the Phosphate-Binding Elements of Escherichia coli Elongation Factor Tu Catalytic Domain," Biochemistry 30 (45), 10872-10877 (1991). ; _Citation_title ; NMR Study of the Phosphate-Binding Elements of Escherichia coli Elongation Factor Tu Catalytic Domain ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lowry David F. . 2 Cool Robert H. . 3 Redfield Alfred G. . 4 Parmeggiani Andrea . . stop_ _Journal_abbreviation Biochemistry _Journal_volume 30 _Journal_issue 45 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 10872 _Page_last 10877 _Year 1991 _Details . save_ ################################## # Molecular system description # ################################## save_system_elongation_factor_Tu _Saveframe_category molecular_system _Mol_system_name 'elongation factor Tu' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'elongation factor Tu' $elongation_factor_Tu stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_elongation_factor_Tu _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'elongation factor Tu' _Name_variant 'catalytic domain,H84G' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 200 _Mol_residue_sequence ; SKEKFERTKPHVNVGTIGHV DHGKTTLTAAITTVLAKTYG GAARAFDQIDNAPEEKARGI TINTSHVEYDTPTRHYAHVD CPGGADYVKNMITGAAQMDG AILVVAATDGPMPQTREHIL LGRQVGVPYIIVFLNKCDMV DDEELLELVEMEVRELLSQY DFPGDDTPIVRGSALKALEG DAEWEAKILELAGFLDSYIP ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 SER 2 2 LYS 3 3 GLU 4 4 LYS 5 5 PHE 6 6 GLU 7 7 ARG 8 8 THR 9 9 LYS 10 10 PRO 11 11 HIS 12 12 VAL 13 13 ASN 14 14 VAL 15 15 GLY 16 16 THR 17 17 ILE 18 18 GLY 19 19 HIS 20 20 VAL 21 21 ASP 22 22 HIS 23 23 GLY 24 24 LYS 25 25 THR 26 26 THR 27 27 LEU 28 28 THR 29 29 ALA 30 30 ALA 31 31 ILE 32 32 THR 33 33 THR 34 34 VAL 35 35 LEU 36 36 ALA 37 37 LYS 38 38 THR 39 39 TYR 40 40 GLY 41 41 GLY 42 42 ALA 43 43 ALA 44 44 ARG 45 45 ALA 46 46 PHE 47 47 ASP 48 48 GLN 49 49 ILE 50 50 ASP 51 51 ASN 52 52 ALA 53 53 PRO 54 54 GLU 55 55 GLU 56 56 LYS 57 57 ALA 58 58 ARG 59 59 GLY 60 60 ILE 61 61 THR 62 62 ILE 63 63 ASN 64 64 THR 65 65 SER 66 66 HIS 67 67 VAL 68 68 GLU 69 69 TYR 70 70 ASP 71 71 THR 72 72 PRO 73 73 THR 74 74 ARG 75 75 HIS 76 76 TYR 77 77 ALA 78 78 HIS 79 79 VAL 80 80 ASP 81 81 CYS 82 82 PRO 83 83 GLY 84 84 GLY 85 85 ALA 86 86 ASP 87 87 TYR 88 88 VAL 89 89 LYS 90 90 ASN 91 91 MET 92 92 ILE 93 93 THR 94 94 GLY 95 95 ALA 96 96 ALA 97 97 GLN 98 98 MET 99 99 ASP 100 100 GLY 101 101 ALA 102 102 ILE 103 103 LEU 104 104 VAL 105 105 VAL 106 106 ALA 107 107 ALA 108 108 THR 109 109 ASP 110 110 GLY 111 111 PRO 112 112 MET 113 113 PRO 114 114 GLN 115 115 THR 116 116 ARG 117 117 GLU 118 118 HIS 119 119 ILE 120 120 LEU 121 121 LEU 122 122 GLY 123 123 ARG 124 124 GLN 125 125 VAL 126 126 GLY 127 127 VAL 128 128 PRO 129 129 TYR 130 130 ILE 131 131 ILE 132 132 VAL 133 133 PHE 134 134 LEU 135 135 ASN 136 136 LYS 137 137 CYS 138 138 ASP 139 139 MET 140 140 VAL 141 141 ASP 142 142 ASP 143 143 GLU 144 144 GLU 145 145 LEU 146 146 LEU 147 147 GLU 148 148 LEU 149 149 VAL 150 150 GLU 151 151 MET 152 152 GLU 153 153 VAL 154 154 ARG 155 155 GLU 156 156 LEU 157 157 LEU 158 158 SER 159 159 GLN 160 160 TYR 161 161 ASP 162 162 PHE 163 163 PRO 164 164 GLY 165 165 ASP 166 166 ASP 167 167 THR 168 168 PRO 169 169 ILE 170 170 VAL 171 171 ARG 172 172 GLY 173 173 SER 174 174 ALA 175 175 LEU 176 176 LYS 177 177 ALA 178 178 LEU 179 179 GLU 180 180 GLY 181 181 ASP 182 182 ALA 183 183 GLU 184 184 TRP 185 185 GLU 186 186 ALA 187 187 LYS 188 188 ILE 189 189 LEU 190 190 GLU 191 191 LEU 192 192 ALA 193 193 GLY 194 194 PHE 195 195 LEU 196 196 ASP 197 197 SER 198 198 TYR 199 199 ILE 200 200 PRO stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-11-25 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1995 "elongation factor Tu" 100.00 200 99.50 99.50 1.14e-142 PDB 1D8T "Crystal Structure Of Elongation Factor, Tu (Ef-Tu-Mggdp) Complexed With Ge2270a, A Thiazolyl Peptide Antibiotic" 100.00 393 99.50 99.50 3.40e-140 PDB 1DG1 "Whole, Unmodified, Ef-Tu(Elongation Factor Tu)." 100.00 394 99.50 99.50 3.22e-140 PDB 1EFC "Intact Elongation Factor From E.Coli" 100.00 393 99.50 99.50 2.95e-140 PDB 1EFU "Elongation Factor Complex Ef-TuEF-Ts From Escherichia Coli" 96.00 385 99.48 99.48 8.76e-134 PDB 1ETU "Structural Details Of The Binding Of Guanosine Diphosphate To Elongation Factor Tu From E. Coli As Studied By X-Ray Crystallogr" 100.00 393 99.50 99.50 3.40e-140 PDB 1LS2 "Fitting Of Ef-Tu And Trna In The Low Resolution Cryo-Em Map Of An Ef-Tu Ternary Complex (Gdp And Kirromycin) Bound To E. Coli 7" 100.00 393 99.50 99.50 2.95e-140 PDB 1OB2 "E. Coli Elongation Factor Ef-tu Complexed With The Antibiotic Kirromycin, A Gtp Analog, And Phe-trna" 100.00 393 99.00 99.50 1.08e-139 PDB 1QZD "Ef-Tu.Kirromycin Coordinates Fitted Into The Cryo-Em Map Of Ef-Tu Ternary Complex (Gdp.Kirromycin) Bound 70s Ribosome" 100.00 393 99.50 99.50 2.95e-140 PDB 2BVN "E. Coli Ef-Tu:gdpnp In Complex With The Antibiotic Enacyloxin Iia" 100.00 393 99.50 99.50 2.95e-140 PDB 2FX3 "Crystal Structure Determination Of E. Coli Elongation Factor, Tu Using A Twinned Data Set" 100.00 393 99.50 99.50 3.40e-140 PDB 2HCJ "trypsin-Modified Elongation Factor Tu In Complex With Tetracycline" 71.00 335 98.59 98.59 2.05e-93 PDB 2HDN "Trypsin-Modified Elongation Factor Tu In Complex With Tetracycline At 2.8 Angstrom Resolution" 71.00 335 99.30 99.30 3.86e-95 PDB 3AGP "Structure Of Viral Polymerase Form I" 100.00 1289 99.50 99.50 1.18e-131 PDB 3AGQ "Structure Of Viral Polymerase Form Ii" 100.00 1289 99.50 99.50 1.18e-131 PDB 3AVT "Structure Of Viral Rna Polymerase Complex 1" 100.00 1289 99.50 99.50 1.18e-131 PDB 3AVU "Structure Of Viral Rna Polymerase Complex 2" 100.00 1289 99.50 99.50 1.18e-131 PDB 3AVV "Structure Of Viral Rna Polymerase Complex 3" 100.00 1289 99.50 99.50 1.18e-131 PDB 3AVW "Structure Of Viral Rna Polymerase Complex 4" 100.00 1289 99.50 99.50 1.18e-131 PDB 3AVX "Structure Of Viral Rna Polymerase Complex 5" 100.00 1289 99.50 99.50 1.18e-131 PDB 3AVY "Structure Of Viral Rna Polymerase Complex 6" 100.00 1289 99.50 99.50 1.18e-131 PDB 3EP2 "Model Of Phe-Trna(Phe) In The Ribosomal Pre-Accommodated State Revealed By Cryo-Em" 100.00 393 99.50 99.50 2.95e-140 PDB 3EQ3 "Model Of Trna(Trp)-Ef-Tu In The Ribosomal Pre-Accommodated State Revealed By Cryo-Em" 100.00 393 99.50 99.50 2.95e-140 PDB 3EQ4 "Model Of Trna(Leu)-Ef-Tu In The Ribosomal Pre-Accommodated State Revealed By Cryo-Em" 100.00 393 99.50 99.50 2.95e-140 PDB 3FIH "Ternary Complex-Bound E.Coli 70s Ribosome. This Entry Consists Of The 30s Subunit, Trnas And The Ternary Complex." 100.00 393 99.50 99.50 2.95e-140 PDB 3IZV "Structural Insights Into Cognate Vs. Near-Cognate Discrimination During Decoding. This Entry Contains The Small Subunit Of A Ri" 100.00 393 99.50 99.50 2.95e-140 PDB 3IZW "Structural Insights Into Cognate Vs. Near-Cognate Discrimination During Decoding.This Entry Contains The Small Subunit Of A Rib" 100.00 393 99.50 99.50 2.95e-140 PDB 3MMP "Structure Of The Qb Replicase, An Rna-Dependent Rna Polymerase Consisting Of Viral And Host Proteins" 100.00 678 99.50 99.50 1.45e-138 PDB 3U2Q "Ef-Tu (Escherichia Coli) In Complex With Nvp-Lff571" 100.00 394 99.00 99.50 1.32e-139 PDB 3U6B "Ef-Tu (Escherichia Coli) In Complex With Nvp-Ldi028" 100.00 394 99.00 99.50 1.32e-139 PDB 3U6K "Ef-Tu (Escherichia Coli) In Complex With Nvp-Ldk733" 100.00 394 99.00 99.50 1.32e-139 PDB 3VNU "Complex Structure Of Viral Rna Polymerase I" 100.00 1289 99.50 99.50 1.18e-131 PDB 3VNV "Complex Structure Of Viral Rna Polymerase Ii" 100.00 1289 99.50 99.50 1.18e-131 PDB 4FWT "Complex Structure Of Viral Rna Polymerase Form Iii" 100.00 1289 99.50 99.50 1.18e-131 PDB 4G5G "Ef-Tu (Escherichia Coli) Complexed With Nvp-Ldu796" 100.00 394 99.00 99.50 1.32e-139 PDB 4P3Y "Crystal Structure Of Acinetobacter Baumannii Dsba In Complex With Ef- Tu" 100.00 394 99.50 99.50 3.71e-140 PDB 4PC1 "Elongation Factor Tu:ts Complex With A Bound Phosphate" 100.00 394 99.50 99.50 3.71e-140 PDB 4PC2 "Elongation Factor Tu:ts Complex With A Bound Gdp" 100.00 394 99.50 99.50 3.71e-140 PDB 4PC3 "Elongation Factor Tu:ts Complex With Partially Bound Gdp" 100.00 394 99.50 99.50 3.71e-140 PDB 4PC6 "Elongation Factor Tu:ts Complex With Bound Gdpnp" 100.00 394 99.50 99.50 3.71e-140 PDB 4PC7 "Elongation Factor Tu:ts Complex In A Near Gtp Conformation." 100.00 394 99.50 99.50 3.71e-140 PDB 4Q7J "Complex Structure Of Viral Rna Polymerase" 100.00 393 99.50 99.50 3.40e-140 PDB 4R71 "Structure Of The Qbeta Holoenzyme Complex In The P1211 Crystal Form" 100.00 694 99.50 99.50 3.08e-138 DBJ BAB37613 "protein chain elongation factor EF-Tu [Escherichia coli O157:H7 str. Sakai]" 100.00 394 99.50 99.50 3.71e-140 DBJ BAB38326 "protein chain elongation factor EF-Tu [Escherichia coli O157:H7 str. Sakai]" 100.00 394 99.50 99.50 3.22e-140 DBJ BAE77340 "protein chain elongation factor EF-Tu [Escherichia coli str. K12 substr. W3110]" 100.00 394 99.50 99.50 3.22e-140 DBJ BAE77952 "protein chain elongation factor EF-Tu [Escherichia coli str. K12 substr. W3110]" 100.00 394 99.50 99.50 3.71e-140 DBJ BAG79124 "translation elongation factor EF-Tu [Escherichia coli SE11]" 100.00 394 99.50 99.50 3.71e-140 EMBL CAA38912 "elongation factor Tu [Salmonella enterica subsp. enterica serovar Typhimurium]" 100.00 394 99.00 99.50 1.23e-139 EMBL CAA38913 "elongation factor Tu [Salmonella enterica subsp. enterica serovar Typhimurium]" 100.00 394 99.00 99.50 1.23e-139 EMBL CAA40370 "translation elongation factor EF-Tu [Escherichia coli]" 100.00 394 99.50 99.50 3.22e-140 EMBL CAD08168 "elongation factor Tu [Salmonella enterica subsp. enterica serovar Typhi str. CT18]" 100.00 394 99.00 99.50 1.23e-139 EMBL CAD09494 "elongation factor Tu [Salmonella enterica subsp. enterica serovar Typhi str. CT18]" 100.00 394 99.00 99.50 1.23e-139 GB AAA24669 "elongation factor Tu [Escherichia coli]" 100.00 394 99.50 99.50 3.22e-140 GB AAA50993 "elongation factor Tu [Escherichia coli]" 100.00 394 99.50 99.50 3.71e-140 GB AAA58136 "CG Site No. 61 [Escherichia coli str. K-12 substr. MG1655]" 100.00 394 99.50 99.50 3.71e-140 GB AAC43078 "elongation factor EF-Tu (duplicate gene) [Escherichia coli str. K-12 substr. MG1655]" 100.00 394 99.50 99.50 3.22e-140 GB AAC76364 "translation elongation factor EF-Tu 1 [Escherichia coli str. K-12 substr. MG1655]" 100.00 394 99.50 99.50 3.71e-140 PIR AD0934 "elongation factor Tu [imported] - Salmonella enterica subsp. enterica serovar Typhi (strain CT18)" 100.00 394 99.00 99.50 1.23e-139 PIR AD1005 "elongation factor Tu [imported] - Salmonella enterica subsp. enterica serovar Typhi (strain CT18)" 100.00 394 99.00 99.50 1.23e-139 PIR D86089 "hypothetical protein tufB [imported] - Escherichia coli (strain O157:H7, substrain EDL933)" 100.00 394 98.00 98.00 1.30e-136 PIR F91152 "protein chain elongation factor EF-Tu [imported] - Escherichia coli (strain O157:H7, substrain RIMD 0509952)" 100.00 394 99.50 99.50 3.71e-140 PRF 0803214A "elongation factor Tu 59-263" 71.00 205 99.30 99.30 6.59e-97 REF NP_312217 "elongation factor Tu [Escherichia coli O157:H7 str. Sakai]" 100.00 394 99.50 99.50 3.71e-140 REF NP_312930 "elongation factor Tu [Escherichia coli O157:H7 str. Sakai]" 100.00 394 99.50 99.50 3.22e-140 REF NP_417798 "translation elongation factor EF-Tu 1 [Escherichia coli str. K-12 substr. MG1655]" 100.00 394 99.50 99.50 3.71e-140 REF NP_418407 "translation elongation factor EF-Tu 2 [Escherichia coli str. K-12 substr. MG1655]" 100.00 394 99.50 99.50 3.22e-140 REF NP_457924 "elongation factor Tu [Salmonella enterica subsp. enterica serovar Typhi str. CT18]" 100.00 394 99.00 99.50 1.23e-139 SP A1AGM6 "RecName: Full=Elongation factor Tu 1; Short=EF-Tu 1" 100.00 394 99.50 99.50 3.71e-140 SP A1AIF3 "RecName: Full=Elongation factor Tu 2; Short=EF-Tu 2" 100.00 394 99.50 99.50 3.22e-140 SP A6TEX7 "RecName: Full=Elongation factor Tu; Short=EF-Tu" 100.00 394 97.50 99.00 2.02e-137 SP A7MKI5 "RecName: Full=Elongation factor Tu; Short=EF-Tu" 100.00 394 98.00 99.00 5.96e-138 SP A7ZSL4 "RecName: Full=Elongation factor Tu 1; Short=EF-Tu 1" 100.00 394 99.50 99.50 3.71e-140 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $elongation_factor_Tu 'E. coli' 562 Eubacteria . Escherichia coli generic stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $elongation_factor_Tu 'not available' . Escherichia coli generic . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.6 . na temperature 290 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS H . . ppm 0 . . . . . $entry_citation $entry_citation 'liquid NH3' N . . ppm 0 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'elongation factor Tu' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 18 GLY H H 6.9 . 1 2 . 18 GLY N N 106.1 . 1 3 . 23 GLY H H 9.09 . 1 4 . 23 GLY N N 109.1 . 1 5 . 24 LYS H H 11.07 . 1 6 . 24 LYS N N 126.8 . 1 7 . 83 GLY H H 11.57 . 1 8 . 83 GLY N N 114.6 . 1 stop_ save_