data_4634 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structural analysis of multi-functional peptide motifs present in human bifunctional tRNA synthetase: Identification of RNA-binding residues and functional implications for tandem repeats ; _BMRB_accession_number 4634 _BMRB_flat_file_name bmr4634.str _Entry_type original _Submission_date 2000-11-09 _Accession_date 2001-03-13 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jeong E.-J. . . 2 Hwang G.-S. . . 3 Kim K. H. . 4 Kim M. J. . 5 Kim S. . . 6 Kim K.-S. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count coupling_constants 1 stop_ loop_ _Data_type _Data_type_count "coupling constants" 46 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2001-05-07 original author . stop_ _Original_release_date 2001-05-07 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structural analysis of multi-functional peptide motifs present in human bifunctional tRNA synthetase: Identification of RNA-binding residues and functional implications for tandem repeats ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 11123902 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jeong E.-J. . . 2 Hwang G.-S. . . 3 Kim K. H. . 4 Kim M. J. . 5 Kim S. . . 6 Kim K.-S. . . stop_ _Journal_abbreviation Biochemistry _Journal_volume 39 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 15775 _Page_last 15782 _Year 2000 _Details . loop_ _Keyword helix-turn-helix stop_ save_ ################################## # Molecular system description # ################################## save_system_tRNA_synthetase _Saveframe_category molecular_system _Mol_system_name 'Multifunctional aminoacyl-tRNA synthetase(E.C.6.1.1.17)' _Abbreviation_common 'tRNA synthetase' _Enzyme_commission_number 6.1.1.17 loop_ _Mol_system_component_name _Mol_label 'Multifunctional aminoacyl-tRNA synthetase' $tRNA_synthetase stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_tRNA_synthetase _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'Multifunctional aminoacyl-tRNA synthetase(E.C.6.1.1.17)' _Abbreviation_common 'tRNA synthetase' _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 57 _Mol_residue_sequence ; DSLVLYNRVAVQGDVVRELK AKKAPKEDVDAAVKQLLSLK AEYKEKTGQEYKPGNPP ; loop_ _Residue_seq_code _Residue_label 1 ASP 2 SER 3 LEU 4 VAL 5 LEU 6 TYR 7 ASN 8 ARG 9 VAL 10 ALA 11 VAL 12 GLN 13 GLY 14 ASP 15 VAL 16 VAL 17 ARG 18 GLU 19 LEU 20 LYS 21 ALA 22 LYS 23 LYS 24 ALA 25 PRO 26 LYS 27 GLU 28 ASP 29 VAL 30 ASP 31 ALA 32 ALA 33 VAL 34 LYS 35 GLN 36 LEU 37 LEU 38 SER 39 LEU 40 LYS 41 ALA 42 GLU 43 TYR 44 LYS 45 GLU 46 LYS 47 THR 48 GLY 49 GLN 50 GLU 51 TYR 52 LYS 53 PRO 54 GLY 55 ASN 56 PRO 57 PRO stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2014-05-12 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1FYJ "Solution Structure Of Multi-Functional Peptide Motif-1 Present In Human Glutamyl-Prolyl Trna Synthetase (Eprs)" 100.00 57 100.00 100.00 1.34e-30 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $tRNA_synthetase human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $tRNA_synthetase 'recombinant technology' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $tRNA_synthetase 2 mM [U-15N] H2O 90 % . D2O 10 % . stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version 1.7 loop_ _Task processing stop_ _Details Delaglio,F. save_ save_pipp _Saveframe_category software _Name PIPP _Version 4.2.5 loop_ _Task 'data analysis' stop_ _Details 'Garretr, D.' save_ save_X-PLOR _Saveframe_category software _Name X-PLOR _Version 3.851 loop_ _Task 'structure solution' stop_ _Details 'Bunger, A.T.' save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model UNITY _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label $sample_1 save_ save_3D_15N-separated_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 15N-separated NOESY' _Sample_label $sample_1 save_ save_DQF-COSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _Sample_label $sample_1 save_ save_HNHA_4 _Saveframe_category NMR_applied_experiment _Experiment_name HNHA _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 . pH pressure 1 . atm temperature 303 . K stop_ save_ ######################## # Coupling constants # ######################## save_coupling_constant_set_1 _Saveframe_category coupling_constants _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_cond_1 _Spectrometer_frequency_1H 600 _Mol_system_component_name 'Multifunctional aminoacyl-tRNA synthetase' _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 3JHNHA 2 SER H 2 SER HA 4.71 . . 0.5 2 3JHNHA 3 LEU H 3 LEU HA 4.83 . . 0.5 3 3JHNHA 4 VAL H 4 VAL HA 2.73 . . 0.5 4 3JHNHA 5 LEU H 5 LEU HA 3.51 . . 0.5 5 3JHNHA 6 TYR H 6 TYR HA 2.97 . . 0.5 6 3JHNHA 7 ASN H 7 ASN HA 3.65 . . 0.5 7 3JHNHA 8 ARG H 8 ARG HA 4.18 . . 0.5 8 3JHNHA 9 VAL H 9 VAL HA 4.70 . . 0.5 9 3JHNHA 10 ALA H 10 ALA HA 2.78 . . 0.5 10 3JHNHA 11 VAL H 11 VAL HA 4.23 . . 0.5 11 3JHNHA 12 GLN H 12 GLN HA 4.25 . . 0.5 12 3JHNHA 14 ASP H 14 ASP HA 4.68 . . 0.5 13 3JHNHA 15 VAL H 15 VAL HA 4.17 . . 0.5 14 3JHNHA 16 VAL H 16 VAL HA 3.56 . . 0.5 15 3JHNHA 17 ARG H 17 ARG HA 3.48 . . 0.5 16 3JHNHA 18 GLU H 18 GLU HA 4.51 . . 0.5 17 3JHNHA 19 LEU H 19 LEU HA 4.16 . . 0.5 18 3JHNHA 20 LYS H 20 LYS HA 4.21 . . 0.5 19 3JHNHA 22 LYS H 22 LYS HA 6.98 . . 0.5 20 3JHNHA 23 LYS H 23 LYS HA 6.41 . . 0.5 21 3JHNHA 24 ALA H 24 ALA HA 4.02 . . 0.5 22 3JHNHA 26 LYS H 26 LYS HA 2.16 . . 0.5 23 3JHNHA 27 GLU H 27 GLU HA 3.17 . . 0.5 24 3JHNHA 28 ASP H 28 ASP HA 6.42 . . 0.5 25 3JHNHA 29 VAL H 29 VAL HA 4.82 . . 0.5 26 3JHNHA 30 ASP H 30 ASP HA 3.20 . . 0.5 27 3JHNHA 31 ALA H 31 ALA HA 3.59 . . 0.5 28 3JHNHA 32 ALA H 32 ALA HA 4.74 . . 0.5 29 3JHNHA 33 VAL H 33 VAL HA 5.12 . . 0.5 30 3JHNHA 34 LYS H 34 LYS HA 3.28 . . 0.5 31 3JHNHA 35 GLN H 35 GLN HA 4.53 . . 0.5 32 3JHNHA 36 LEU H 36 LEU HA 3.09 . . 0.5 33 3JHNHA 37 LEU H 37 LEU HA 3.19 . . 0.5 34 3JHNHA 38 SER H 38 SER HA 4.15 . . 0.5 35 3JHNHA 40 LYS H 40 LYS HA 3.38 . . 0.5 36 3JHNHA 41 ALA H 41 ALA HA 4.55 . . 0.5 37 3JHNHA 43 TYR H 43 TYR HA 3.06 . . 0.5 38 3JHNHA 44 LYS H 44 LYS HA 3.84 . . 0.5 39 3JHNHA 45 GLU H 45 GLU HA 3.26 . . 0.5 40 3JHNHA 46 LYS H 46 LYS HA 4.89 . . 0.5 41 3JHNHA 47 THR H 47 THR HA 7.50 . . 0.5 42 3JHNHA 49 GLN H 49 GLN HA 8.34 . . 0.5 43 3JHNHA 50 GLU H 50 GLU HA 5.44 . . 0.5 44 3JHNHA 51 TYR H 51 TYR HA 3.98 . . 0.5 45 3JHNHA 52 LYS H 52 LYS HA 4.79 . . 0.5 46 3JHNHA 55 ASN H 55 ASN HA 8.04 . . 0.5 stop_ save_