data_5353 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H, 15N and 13C resonance assignments of yeast Saccharomyces cerevisiae calmodulin in the Ca2+-free state ; _BMRB_accession_number 5353 _BMRB_flat_file_name bmr5353.str _Entry_type original _Submission_date 2002-04-25 _Accession_date 2002-04-25 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ishida Hiroaki . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 142 "15N chemical shifts" 141 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2002-09-25 original author . stop_ _Original_release_date 2002-09-25 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Letter to the Editor: 1H, 15N and 13C resonance assignments of yeast Saccharomyces cerevisiae calmodulin in the Ca2+-free state ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Ishida Hiroaki . . 2 Nakashima K-i . . 3 Kumaki Yasuhiro . . 4 Nakata Mitsuo . . 5 Hikichi Kunio . . 6 Yazawa Michio . . stop_ _Journal_abbreviation 'J. Biomol. NMR' _Journal_volume 23 _Journal_issue 4 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 323 _Page_last 324 _Year 2002 _Details . loop_ _Keyword 'Yeast Saccharomyces cerevisiae Calmodulin' EF-hand stop_ save_ ################################## # Molecular system description # ################################## save_system_yCaM _Saveframe_category molecular_system _Mol_system_name 'yeast Saccharomyces cerevisiae calmodulin' _Abbreviation_common yCaM _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'yCaM monomer' $yCaM stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_yCaM _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'yeast calmodulin' _Abbreviation_common yCaM _Molecular_mass 16000 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 146 _Mol_residue_sequence ; SSNLTEEQIAEFKEAFALFD KDNNGSISSSELATVMRSLG LSPSEAEVNDLMNEIDVDGN HQIEFSEFLALMSRQLKSND SEQELLEAFKVFDKNGDGLI SAAELKHVLTSIGEKLTDAE VDDMLREVSDGSGEINIQQF AALLSK ; loop_ _Residue_seq_code _Residue_label 1 SER 2 SER 3 ASN 4 LEU 5 THR 6 GLU 7 GLU 8 GLN 9 ILE 10 ALA 11 GLU 12 PHE 13 LYS 14 GLU 15 ALA 16 PHE 17 ALA 18 LEU 19 PHE 20 ASP 21 LYS 22 ASP 23 ASN 24 ASN 25 GLY 26 SER 27 ILE 28 SER 29 SER 30 SER 31 GLU 32 LEU 33 ALA 34 THR 35 VAL 36 MET 37 ARG 38 SER 39 LEU 40 GLY 41 LEU 42 SER 43 PRO 44 SER 45 GLU 46 ALA 47 GLU 48 VAL 49 ASN 50 ASP 51 LEU 52 MET 53 ASN 54 GLU 55 ILE 56 ASP 57 VAL 58 ASP 59 GLY 60 ASN 61 HIS 62 GLN 63 ILE 64 GLU 65 PHE 66 SER 67 GLU 68 PHE 69 LEU 70 ALA 71 LEU 72 MET 73 SER 74 ARG 75 GLN 76 LEU 77 LYS 78 SER 79 ASN 80 ASP 81 SER 82 GLU 83 GLN 84 GLU 85 LEU 86 LEU 87 GLU 88 ALA 89 PHE 90 LYS 91 VAL 92 PHE 93 ASP 94 LYS 95 ASN 96 GLY 97 ASP 98 GLY 99 LEU 100 ILE 101 SER 102 ALA 103 ALA 104 GLU 105 LEU 106 LYS 107 HIS 108 VAL 109 LEU 110 THR 111 SER 112 ILE 113 GLY 114 GLU 115 LYS 116 LEU 117 THR 118 ASP 119 ALA 120 GLU 121 VAL 122 ASP 123 ASP 124 MET 125 LEU 126 ARG 127 GLU 128 VAL 129 SER 130 ASP 131 GLY 132 SER 133 GLY 134 GLU 135 ILE 136 ASN 137 ILE 138 GLN 139 GLN 140 PHE 141 ALA 142 ALA 143 LEU 144 LEU 145 SER 146 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2014-10-26 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 17850 yCaM_GS_CNA1 100.00 176 100.00 100.00 4.00e-96 PDB 1LKJ "Nmr Structure Of Apo Calmodulin From Yeast Saccharomyces Cerevisiae" 100.00 146 100.00 100.00 5.42e-96 PDB 2LHI "Solution Structure Of Ca2+CNA1 PEPTIDE-Bound Ycam" 100.00 176 100.00 100.00 4.00e-96 DBJ GAA21657 "K7_Cmd1p [Saccharomyces cerevisiae Kyokai no. 7]" 100.00 147 99.32 99.32 2.76e-95 EMBL CAA55612 "calmodulin [Saccharomyces cerevisiae]" 100.00 147 100.00 100.00 5.86e-96 EMBL CAA85064 "CMD1 [Saccharomyces cerevisiae]" 100.00 147 100.00 100.00 5.86e-96 EMBL CAG58389 "unnamed protein product [Candida glabrata]" 100.00 147 98.63 99.32 1.60e-94 EMBL CAR27417 "ZYRO0C16346p [Zygosaccharomyces rouxii]" 100.00 148 97.26 99.32 1.81e-93 EMBL CBK39182 "Cmd1p [Saccharomyces cerevisiae EC1118]" 100.00 147 100.00 100.00 5.86e-96 GB AAA34504 "calmodulin [Saccharomyces cerevisiae]" 100.00 147 100.00 100.00 5.86e-96 GB AAC68888 "calmodulin [Saccharomyces cerevisiae]" 100.00 147 100.00 100.00 5.86e-96 GB AAS56510 "YBR109C [Saccharomyces cerevisiae]" 100.00 147 100.00 100.00 5.86e-96 GB AHY74589 "Cmd1p [Saccharomyces cerevisiae YJM993]" 100.00 147 100.00 100.00 5.86e-96 GB EDN64723 "calmodulin [Saccharomyces cerevisiae YJM789]" 100.00 147 100.00 100.00 5.86e-96 REF NP_009667 "calmodulin [Saccharomyces cerevisiae S288c]" 100.00 147 100.00 100.00 5.86e-96 REF XP_001642859 "hypothetical protein Kpol_376p14 [Vanderwaltozyma polyspora DSM 70294]" 100.00 147 97.26 99.32 2.23e-92 REF XP_002496350 "ZYRO0C16346p [Zygosaccharomyces rouxii]" 100.00 148 97.26 99.32 1.81e-93 REF XP_003678123 "hypothetical protein NCAS_0I01100 [Naumovozyma castellii CBS 4309]" 100.00 147 97.26 99.32 2.23e-93 REF XP_003680602 "hypothetical protein TDEL_0C05020 [Torulaspora delbrueckii]" 100.00 147 97.95 99.32 5.10e-94 SP P06787 "RecName: Full=Calmodulin; Short=CaM [Saccharomyces cerevisiae S288c]" 100.00 147 100.00 100.00 5.86e-96 TPG DAA07228 "TPA: calmodulin [Saccharomyces cerevisiae S288c]" 100.00 147 100.00 100.00 5.86e-96 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $yCaM 'Baker's yeast' 4932 Eukaryota Fungi Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $yCaM 'recombinant technology' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $yCaM 1.0 mM '[U-13C; U-15N]' $yCaM 1.4 mM '[U-13C; U-15N]' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $yCaM 1.0 mM [U-15N] $yCaM 1.4 mM [U-15N] stop_ save_ ############################ # Computer software used # ############################ save_XEASY _Saveframe_category software _Name XEASY _Version . loop_ _Task 'peak assignments' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model UNITY _Field_strength 500 _Details . save_ save_NMR_spectrometer2 _Saveframe_category NMR_spectrometer _Manufacturer JEOL _Model JNM _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name HSQC _Sample_label . save_ save_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name HNCA _Sample_label . save_ save_HN(CO)CA_3 _Saveframe_category NMR_applied_experiment _Experiment_name HN(CO)CA _Sample_label . save_ save_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name HNCACB _Sample_label . save_ save_CBCA(CO)NH_5 _Saveframe_category NMR_applied_experiment _Experiment_name CBCA(CO)NH _Sample_label . save_ save_HNHA_6 _Saveframe_category NMR_applied_experiment _Experiment_name HNHA _Sample_label . save_ save_HNCO_7 _Saveframe_category NMR_applied_experiment _Experiment_name HNCO _Sample_label . save_ save_HBHA(CBCACO)NH_8 _Saveframe_category NMR_applied_experiment _Experiment_name HBHA(CBCACO)NH _Sample_label . save_ save_H(CCO)NH_9 _Saveframe_category NMR_applied_experiment _Experiment_name H(CCO)NH _Sample_label . save_ save_C(CO)NH_10 _Saveframe_category NMR_applied_experiment _Experiment_name C(CO)NH _Sample_label . save_ save_HCCH-TOCSY_11 _Saveframe_category NMR_applied_experiment _Experiment_name HCCH-TOCSY _Sample_label . save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name HSQC _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name HNCA _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name HN(CO)CA _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_4 _Saveframe_category NMR_applied_experiment _Experiment_name HNCACB _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_5 _Saveframe_category NMR_applied_experiment _Experiment_name CBCA(CO)NH _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_6 _Saveframe_category NMR_applied_experiment _Experiment_name HNHA _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_7 _Saveframe_category NMR_applied_experiment _Experiment_name HNCO _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_8 _Saveframe_category NMR_applied_experiment _Experiment_name HBHA(CBCACO)NH _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_9 _Saveframe_category NMR_applied_experiment _Experiment_name H(CCO)NH _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_10 _Saveframe_category NMR_applied_experiment _Experiment_name C(CO)NH _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_11 _Saveframe_category NMR_applied_experiment _Experiment_name HCCH-TOCSY _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_experimental_conditions _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.0 0.1 n/a temperature 303 0.1 K 'ionic strength' 0.05 . M pressure 1 . atm stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 DSS N 15 'methyl protons' ppm 0.0 . indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $experimental_conditions _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'yCaM monomer' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 3 ASN N N 120.14 . . 2 . 3 ASN H H 8.53 . . 3 . 4 LEU N N 120.84 . . 4 . 4 LEU H H 8.10 . . 5 . 5 THR N N 113.50 . . 6 . 5 THR H H 8.81 . . 7 . 6 GLU N N 119.98 . . 8 . 6 GLU H H 9.01 . . 9 . 7 GLU N N 119.54 . . 10 . 7 GLU H H 8.72 . . 11 . 8 GLN N N 119.98 . . 12 . 8 GLN H H 7.74 . . 13 . 9 ILE N N 118.25 . . 14 . 9 ILE H H 8.21 . . 15 . 10 ALA N N 120.41 . . 16 . 10 ALA H H 7.91 . . 17 . 11 GLU N N 119.11 . . 18 . 11 GLU H H 7.80 . . 19 . 12 PHE N N 119.98 . . 20 . 12 PHE H H 8.84 . . 21 . 13 LYS N N 120.84 . . 22 . 13 LYS H H 9.12 . . 23 . 14 GLU N N 118.25 . . 24 . 14 GLU H H 7.88 . . 25 . 15 ALA N N 120.84 . . 26 . 15 ALA H H 7.74 . . 27 . 16 PHE N N 117.82 . . 28 . 16 PHE H H 8.46 . . 29 . 17 ALA N N 118.05 . . 30 . 17 ALA H H 8.27 . . 31 . 18 LEU N N 116.52 . . 32 . 18 LEU H H 7.35 . . 33 . 19 PHE N N 115.23 . . 34 . 19 PHE H H 7.48 . . 35 . 20 ASP N N 123.00 . . 36 . 20 ASP H H 7.60 . . 37 . 21 LYS N N 122.57 . . 38 . 21 LYS H H 7.91 . . 39 . 22 ASP N N 116.95 . . 40 . 22 ASP H H 8.64 . . 41 . 23 ASN N N 117.84 . . 42 . 23 ASN H H 7.98 . . 43 . 24 ASN N N 118.16 . . 44 . 24 ASN H H 9.87 . . 45 . 25 GLY H H 10.72 . . 46 . 25 GLY N N 112.52 . . 47 . 26 SER N N 113.50 . . 48 . 26 SER H H 7.70 . . 49 . 27 ILE N N 111.42 . . 50 . 27 ILE H H 8.16 . . 51 . 28 SER N N 116.52 . . 52 . 28 SER H H 8.71 . . 53 . 29 SER N N 121.27 . . 54 . 29 SER H H 8.99 . . 55 . 30 SER N N 115.66 . . 56 . 30 SER H H 8.10 . . 57 . 31 GLU N N 120.41 . . 58 . 31 GLU H H 7.77 . . 59 . 32 LEU N N 120.72 . . 60 . 32 LEU H H 7.59 . . 61 . 33 ALA N N 119.54 . . 62 . 33 ALA H H 8.77 . . 63 . 34 THR N N 114.79 . . 64 . 34 THR H H 7.54 . . 65 . 35 VAL N N 123.00 . . 66 . 35 VAL H H 8.04 . . 67 . 36 MET N N 118.68 . . 68 . 36 MET H H 8.47 . . 69 . 37 ARG N N 118.68 . . 70 . 37 ARG H H 8.36 . . 71 . 38 SER N N 118.25 . . 72 . 38 SER H H 8.17 . . 73 . 39 LEU N N 120.41 . . 74 . 39 LEU H H 7.39 . . 75 . 40 GLY N N 106.16 . . 76 . 40 GLY H H 7.91 . . 77 . 41 LEU N N 119.98 . . 78 . 41 LEU H H 7.70 . . 79 . 42 SER N N 113.50 . . 80 . 42 SER H H 8.66 . . 81 . 44 SER N N 118.25 . . 82 . 44 SER H H 8.92 . . 83 . 45 GLU N N 121.27 . . 84 . 45 GLU H H 8.83 . . 85 . 46 ALA N N 120.84 . . 86 . 46 ALA H H 8.29 . . 87 . 47 GLU N N 118.25 . . 88 . 47 GLU H H 7.78 . . 89 . 48 VAL N N 119.54 . . 90 . 48 VAL H H 8.21 . . 91 . 49 ASN N N 118.68 . . 92 . 49 ASN H H 8.21 . . 93 . 50 ASP N N 119.54 . . 94 . 50 ASP H H 8.10 . . 95 . 51 LEU N N 120.84 . . 96 . 51 LEU H H 7.95 . . 97 . 52 MET N N 116.09 . . 98 . 52 MET H H 8.33 . . 99 . 53 ASN N N 116.09 . . 100 . 53 ASN H H 7.94 . . 101 . 54 GLU N N 118.25 . . 102 . 54 GLU H H 7.84 . . 103 . 55 ILE N N 115.23 . . 104 . 55 ILE H H 7.72 . . 105 . 56 ASP N N 121.70 . . 106 . 56 ASP H H 8.23 . . 107 . 57 VAL N N 119.98 . . 108 . 57 VAL H H 7.99 . . 109 . 58 ASP N N 120.41 . . 110 . 58 ASP H H 8.36 . . 111 . 59 GLY N N 107.88 . . 112 . 59 GLY H H 8.08 . . 113 . 60 ASN N N 117.82 . . 114 . 60 ASN H H 8.35 . . 115 . 61 HIS N N 118.25 . . 116 . 61 HIS H H 8.82 . . 117 . 62 GLN N N 120.41 . . 118 . 62 GLN H H 8.28 . . 119 . 63 ILE N N 121.70 . . 120 . 63 ILE H H 9.24 . . 121 . 64 GLU N N 125.59 . . 122 . 64 GLU H H 8.95 . . 123 . 65 PHE N N 122.57 . . 124 . 65 PHE H H 8.74 . . 125 . 66 SER N N 112.63 . . 126 . 66 SER H H 9.04 . . 127 . 67 GLU N N 123.00 . . 128 . 67 GLU H H 7.12 . . 129 . 68 PHE N N 121.70 . . 130 . 68 PHE H H 8.27 . . 131 . 69 LEU N N 117.82 . . 132 . 69 LEU H H 8.33 . . 133 . 70 ALA N N 121.70 . . 134 . 70 ALA H H 7.35 . . 135 . 71 LEU N N 119.54 . . 136 . 71 LEU H H 7.95 . . 137 . 72 MET N N 117.84 . . 138 . 72 MET H H 8.12 . . 139 . 73 SER N N 113.50 . . 140 . 73 SER H H 8.19 . . 141 . 74 ARG N N 119.11 . . 142 . 74 ARG H H 7.43 . . 143 . 75 GLN N N 117.38 . . 144 . 75 GLN H H 7.90 . . 145 . 76 LEU N N 119.11 . . 146 . 76 LEU H H 7.80 . . 147 . 77 LYS N N 119.98 . . 148 . 77 LYS H H 7.86 . . 149 . 78 SER N N 116.09 . . 150 . 78 SER H H 8.29 . . 151 . 79 ASN N N 120.84 . . 152 . 79 ASN H H 8.48 . . 153 . 80 ASP N N 120.84 . . 154 . 80 ASP H H 8.32 . . 155 . 81 SER N N 116.09 . . 156 . 81 SER H H 8.37 . . 157 . 82 GLU N N 122.13 . . 158 . 82 GLU H H 8.43 . . 159 . 83 GLN N N 118.25 . . 160 . 83 GLN H H 8.17 . . 161 . 84 GLU N N 119.98 . . 162 . 84 GLU H H 8.27 . . 163 . 85 LEU N N 121.27 . . 164 . 85 LEU H H 8.08 . . 165 . 86 LEU N N 118.68 . . 166 . 86 LEU H H 8.05 . . 167 . 87 GLU N N 117.82 . . 168 . 87 GLU H H 8.10 . . 169 . 88 ALA N N 121.70 . . 170 . 88 ALA H H 7.89 . . 171 . 89 PHE N N 115.23 . . 172 . 89 PHE H H 8.10 . . 173 . 90 LYS N N 118.68 . . 174 . 90 LYS H H 7.97 . . 175 . 91 VAL N N 116.52 . . 176 . 91 VAL H H 7.40 . . 177 . 93 ASP N N 120.41 . . 178 . 93 ASP H H 7.86 . . 179 . 94 LYS N N 122.57 . . 180 . 94 LYS H H 8.21 . . 181 . 95 ASN N N 116.09 . . 182 . 95 ASN H H 8.64 . . 183 . 96 GLY N N 109.18 . . 184 . 96 GLY H H 8.14 . . 185 . 97 ASP N N 119.54 . . 186 . 97 ASP H H 8.32 . . 187 . 98 GLY H H 9.87 . . 188 . 99 LEU N N 120.84 . . 189 . 99 LEU H H 7.84 . . 190 . 100 ILE N N 122.80 . . 191 . 100 ILE H H 8.53 . . 192 . 101 SER N N 119.54 . . 193 . 101 SER H H 8.53 . . 194 . 102 ALA N N 125.16 . . 195 . 102 ALA H H 8.72 . . 196 . 103 ALA N N 118.25 . . 197 . 103 ALA H H 8.27 . . 198 . 104 GLU N N 118.68 . . 199 . 104 GLU H H 7.74 . . 200 . 105 LEU N N 120.84 . . 201 . 105 LEU H H 8.14 . . 202 . 106 LYS N N 117.38 . . 203 . 106 LYS H H 8.19 . . 204 . 107 HIS N N 117.82 . . 205 . 107 HIS H H 7.72 . . 206 . 108 VAL N N 120.41 . . 207 . 108 VAL H H 8.13 . . 208 . 109 LEU N N 118.68 . . 209 . 109 LEU H H 8.34 . . 210 . 110 THR N N 111.77 . . 211 . 110 THR H H 8.01 . . 212 . 111 SER N N 117.38 . . 213 . 111 SER H H 7.95 . . 214 . 112 ILE N N 116.95 . . 215 . 112 ILE H H 7.70 . . 216 . 113 GLY N N 109.18 . . 217 . 113 GLY H H 8.00 . . 218 . 114 GLU N N 119.98 . . 219 . 114 GLU H H 8.07 . . 220 . 115 LYS N N 121.27 . . 221 . 115 LYS H H 8.43 . . 222 . 116 LEU N N 122.13 . . 223 . 116 LEU H H 7.88 . . 224 . 117 THR N N 113.50 . . 225 . 117 THR H H 8.82 . . 226 . 118 ASP N N 120.41 . . 227 . 118 ASP H H 8.62 . . 228 . 119 ALA N N 120.41 . . 229 . 119 ALA H H 8.19 . . 230 . 120 GLU N N 118.75 . . 231 . 120 GLU H H 7.80 . . 232 . 121 VAL N N 120.41 . . 233 . 121 VAL H H 8.18 . . 234 . 122 ASP N N 120.41 . . 235 . 122 ASP H H 8.31 . . 236 . 123 ASP N N 119.11 . . 237 . 123 ASP H H 8.04 . . 238 . 124 MET N N 119.98 . . 239 . 124 MET H H 7.98 . . 240 . 125 LEU N N 117.82 . . 241 . 125 LEU H H 8.45 . . 242 . 126 ARG N N 119.11 . . 243 . 126 ARG H H 7.90 . . 244 . 127 GLU N N 117.38 . . 245 . 127 GLU H H 7.84 . . 246 . 128 VAL N N 113.26 . . 247 . 128 VAL H H 7.74 . . 248 . 129 SER N N 116.09 . . 249 . 129 SER H H 7.89 . . 250 . 130 ASP N N 122.57 . . 251 . 130 ASP H H 8.48 . . 252 . 131 GLY N N 107.88 . . 253 . 131 GLY H H 8.38 . . 254 . 132 SER N N 116.09 . . 255 . 132 SER H H 8.44 . . 256 . 133 GLY N N 110.04 . . 257 . 133 GLY H H 8.46 . . 258 . 134 GLU N N 119.98 . . 259 . 134 GLU H H 8.13 . . 260 . 135 ILE N N 121.49 . . 261 . 135 ILE H H 8.99 . . 262 . 136 ASN N N 123.43 . . 263 . 136 ASN H H 8.58 . . 264 . 137 ILE N N 124.40 . . 265 . 137 ILE H H 8.50 . . 266 . 138 GLN N N 120.84 . . 267 . 138 GLN H H 8.33 . . 268 . 139 GLN N N 119.54 . . 269 . 139 GLN H H 8.22 . . 270 . 140 PHE N N 119.54 . . 271 . 140 PHE H H 8.26 . . 272 . 141 ALA N N 119.98 . . 273 . 141 ALA H H 8.51 . . 274 . 142 ALA N N 118.68 . . 275 . 142 ALA H H 7.66 . . 276 . 143 LEU N N 118.25 . . 277 . 143 LEU H H 7.69 . . 278 . 144 LEU N N 117.38 . . 279 . 144 LEU H H 7.52 . . 280 . 145 SER N N 113.93 . . 281 . 145 SER H H 7.61 . . 282 . 146 LYS N N 127.32 . . 283 . 146 LYS H H 7.69 . . stop_ save_