data_5773 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of HIV-1 Stem Loop SL1 ; _BMRB_accession_number 5773 _BMRB_flat_file_name bmr5773.str _Entry_type original _Submission_date 2003-03-15 _Accession_date 2003-03-15 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lawrence D. C. . 2 Stover C. C. . 3 Noznitsky J. . . 4 Wu Z. . . 5 Summers M. F. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 272 "13C chemical shifts" 237 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2003-05-01 original author . stop_ _Original_release_date 2003-05-01 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structure of the Intact Stem and Bulge of HIV-1 Psi-RNA Stem Loop SL1' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 12559920 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lawrence D. C. . 2 Stover C. C. . 3 Noznitsky J. . . 4 Wu Z. . . 5 Summers M. F. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 326 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 529 _Page_last 542 _Year 2003 _Details . loop_ _Keyword BULGE 'G-A MISMATCH' HIV 'RNA STEM LOOP' SL1 stop_ save_ ################################## # Molecular system description # ################################## save_system_HIV-1_SL1 _Saveframe_category molecular_system _Mol_system_name 'HIV-1 STEM LOOP SL1 MONOMERIC RNA' _Abbreviation_common 'HIV-1 SL1' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'HIV-1 STEM LOOP SL1 MONOMERIC RNA' $SL1 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'not present' loop_ _Biological_function 'dimerization of HIV-1 genome' 'part of Psi encapsidation signal' stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_SL1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common 'HIV-1 Stem loop SL1' _Name_variant SL1m _Abbreviation_common SL1 _Molecular_mass 12363 _Mol_thiol_state 'not present' _Details ; G-A mismatched base pair. DIS terminal loop replaced with GAGA tetraloop. ; ############################## # Polymer residue sequence # ############################## _Residue_count 36 _Mol_residue_sequence ; GGACUCGGCUUGCUGGAGAC GGCAAGAGGCGAGUCC ; loop_ _Residue_seq_code _Residue_label 1 G 2 G 3 A 4 C 5 U 6 C 7 G 8 G 9 C 10 U 11 U 12 G 13 C 14 U 15 G 16 G 17 A 18 G 19 A 20 C 21 G 22 G 23 C 24 A 25 A 26 G 27 A 28 G 29 G 30 C 31 G 32 A 33 G 34 U 35 C 36 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $SL1 HIV-1 11676 Viruses . Lentivirus HIV-1 Lai stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $SL1 'enzymatic semisynthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SL1 1.0 mM '[U-13C; U-15N]' Tris-Cl 10 mM . Na-EDTA 0.1 mM . D2O 100 % . stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SL1 1.0 mM '[13C, 15N]-cytidine' Tris-Cl 10 mM . Na-EDTA 0.1 mM . D2O 100 % . stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SL1 1.0 mM . Tris-Cl 10 mM . Na-EDTA 0.1 mM . D2O 100 % . stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SL1 1.0 mM '[U-13C; U-15N]' Tris-Cl 10 mM . Na-EDTA 0.1 mM . 'Pf1 phage' 17 mg/ml . stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SL1 1.0 mM . Tris-Cl 10 mM . Na-EDTA 0.1 mM . D2O 10 % . H2O 90 % . stop_ save_ ############################ # Computer software used # ############################ save_XWINNMR _Saveframe_category software _Name xwinnmr _Version 2.6 loop_ _Task collection stop_ _Details Bruker save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version 2.1 loop_ _Task processing stop_ _Details ; Delaglio, Grzesiek, Vuister, Zhu, Pfeifer,Bax (1995). NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J. Biomol. NMR, 6, 277-293. ; save_ save_NMRView _Saveframe_category software _Name NMRView _Version 5.0.3 loop_ _Task 'data analysis' stop_ _Details ; Johnson, Blevins (1994). NMRview: a computer program for the visualization and analysis for NMR data. J. Biomol. NMR, 4, 603-614. ; save_ save_DYANA _Saveframe_category software _Name DYANA _Version 1.5 loop_ _Task refinement stop_ _Details ; Guntert, Mumenthaler, Wuthrich (1997) Torsion angle dynamics for protein structures calculations with a new program, DYANA. J. Mol. Biol., 273, 283-298. ; save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 600 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_13C-separated_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 13C-separated NOESY' _Sample_label . save_ save_4D_13C-separated_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '4D 13C-separated NOESY' _Sample_label . save_ save_3D_13C-separated_ROESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 13C-separated ROESY' _Sample_label . save_ save_IPAP_H-COUPLED_CT-HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name 'IPAP H-COUPLED CT-HSQC' _Sample_label . save_ save_2D_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label . save_ save_2D_ROESY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D ROESY' _Sample_label . save_ ####################### # Sample conditions # ####################### save_sample_cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.0 0.1 n/a pressure 1 . atm temperature 308 0.2 K stop_ save_ save_sample_cond_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 8.0 0.05 n/a pressure 1 . atm temperature 308 0.2 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS C 13 'methyl protons' ppm 0.0 . indirect . . . 0.251449530 $entry_citation $entry_citation DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.000000000 $entry_citation $entry_citation DSS N 15 'methyl protons' ppm 0.0 . indirect . . . 0.101329118 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'HIV-1 STEM LOOP SL1 MONOMERIC RNA' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 G C1' C 84.312 . 1 2 . 1 G H1' H 5.881 . 1 3 . 1 G C2' C 64.943 . 1 4 . 1 G H2' H 4.990 . 1 5 . 1 G C3' C 65.319 . 1 6 . 1 G H3' H 4.800 . 1 7 . 1 G C4' C 73.744 . 1 8 . 1 G H4' H 4.614 . 1 9 . 1 G C5' C 57.386 . 1 10 . 1 G H5' H 4.434 . 2 11 . 1 G H5'' H 4.339 . 2 12 . 1 G C8 C 136.868 . 1 13 . 1 G H8 H 8.176 . 1 14 . 2 G C1' C 86.568 . 1 15 . 2 G H1' H 5.952 . 1 16 . 2 G C2' C 65.401 . 1 17 . 2 G H2' H 4.767 . 1 18 . 2 G C3' C 62.926 . 1 19 . 2 G H3' H 4.689 . 1 20 . 2 G C4' C 72.769 . 1 21 . 2 G H4' H 4.650 . 1 22 . 2 G C5' C 53.019 . 1 23 . 2 G H5' H 4.521 . 2 24 . 2 G H5'' H 4.414 . 2 25 . 2 G C8 C 135.099 . 1 26 . 2 G H8 H 7.671 . 1 27 . 3 A C1' C 90.838 . 1 28 . 3 A H1' H 6.081 . 1 29 . 3 A C2' C 65.245 . 1 30 . 3 A H2' H 4.695 . 1 31 . 3 A C3' C 62.835 . 1 32 . 3 A H3' H 4.680 . 1 33 . 3 A C4' C 72.056 . 1 34 . 3 A H4' H 4.598 . 1 35 . 3 A C5' C 51.186 . 1 36 . 3 A H5' H 4.652 . 2 37 . 3 A H5'' H 4.251 . 2 38 . 3 A C8 C 137.163 . 1 39 . 3 A H8 H 7.923 . 1 40 . 3 A C2 C 151.856 . 1 41 . 3 A H2 H 7.875 . 1 42 . 4 C C1' C 87.861 . 1 43 . 4 C H1' H 5.427 . 1 44 . 4 C C2' C 65.455 . 1 45 . 4 C H2' H 4.336 . 1 46 . 4 C C3' C 62.532 . 1 47 . 4 C H3' H 4.356 . 1 48 . 4 C C4' C 71.834 . 1 49 . 4 C H4' H 4.493 . 1 50 . 4 C C5' C 51.140 . 1 51 . 4 C H5' H 4.539 . 2 52 . 4 C H5'' H 4.140 . 2 53 . 4 C C6 C 138.429 . 1 54 . 4 C H6 H 7.419 . 1 55 . 4 C C5 C 91.292 . 1 56 . 4 C H5 H 5.307 . 1 57 . 5 U C1' C 87.748 . 1 58 . 5 U H1' H 5.619 . 1 59 . 5 U C2' C 65.284 . 1 60 . 5 U H2' H 4.566 . 1 61 . 5 U C3' C 62.294 . 1 62 . 5 U H3' H 4.588 . 1 63 . 5 U C4' C 72.067 . 1 64 . 5 U H4' H 4.502 . 1 65 . 5 U C5' C 50.734 . 1 66 . 5 U H5' H 4.599 . 2 67 . 5 U H5'' H 4.172 . 2 68 . 5 U C6 C 139.954 . 1 69 . 5 U H6 H 7.902 . 1 70 . 5 U C5 C 97.224 . 1 71 . 5 U H5 H 5.427 . 1 72 . 6 C C1' C 87.564 . 1 73 . 6 C H1' H 5.612 . 1 74 . 6 C C2' C 65.416 . 1 75 . 6 C H2' H 4.506 . 1 76 . 6 C C3' C 62.112 . 1 77 . 6 C H3' H 4.586 . 1 78 . 6 C C4' C 72.107 . 1 79 . 6 C H4' H 4.503 . 1 80 . 6 C C5' C 50.816 . 1 81 . 6 C H5' H 4.600 . 2 82 . 6 C H5'' H 4.195 . 2 83 . 6 C C6 C 139.136 . 1 84 . 6 C H6 H 7.869 . 1 85 . 6 C C5 C 91.720 . 1 86 . 6 C H5 H 5.699 . 1 87 . 7 G C1' C 86.767 . 1 88 . 7 G H1' H 5.711 . 1 89 . 7 G C2' C 65.269 . 1 90 . 7 G H2' H 4.661 . 1 91 . 7 G C3' C 63.259 . 1 92 . 7 G H3' H 4.676 . 1 93 . 7 G C4' C 72.495 . 1 94 . 7 G H4' H 4.480 . 1 95 . 7 G C5' C 51.392 . 1 96 . 7 G H5' H 4.557 . 2 97 . 7 G H5'' H 4.197 . 2 98 . 7 G C8 C 134.426 . 1 99 . 7 G H8 H 7.617 . 1 100 . 8 G C1' C 85.984 . 1 101 . 8 G H1' H 5.827 . 1 102 . 8 G C2' C 65.271 . 1 103 . 8 G H2' H 4.716 . 1 104 . 8 G C3' C 65.273 . 1 105 . 8 G H3' H 4.672 . 1 106 . 8 G C4' C 73.886 . 1 107 . 8 G H4' H 4.582 . 1 108 . 8 G C5' C 52.325 . 1 109 . 8 G H5' H 4.473 . 2 110 . 8 G H5'' H 4.316 . 2 111 . 8 G C8 C 136.403 . 1 112 . 8 G H8 H 7.607 . 1 113 . 9 C C1' C 87.819 . 1 114 . 9 C H1' H 5.601 . 1 115 . 9 C C2' C 65.212 . 1 116 . 9 C H2' H 4.486 . 1 117 . 9 C C3' C 62.311 . 1 118 . 9 C H3' H 4.552 . 1 119 . 9 C C4' C 72.090 . 1 120 . 9 C H4' H 4.499 . 1 121 . 9 C C5' C 51.072 . 1 122 . 9 C H5' H 4.554 . 2 123 . 9 C H5'' H 4.217 . 2 124 . 9 C C6 C 139.716 . 1 125 . 9 C H6 H 7.785 . 1 126 . 9 C C5 C 91.742 . 1 127 . 9 C H5 H 5.631 . 1 128 . 10 U C1' C 87.951 . 1 129 . 10 U H1' H 5.597 . 1 130 . 10 U C2' C 65.344 . 1 131 . 10 U H2' H 4.514 . 1 132 . 10 U C3' C 62.605 . 1 133 . 10 U H3' H 4.597 . 1 134 . 10 U C4' C 72.147 . 1 135 . 10 U H4' H 4.503 . 1 136 . 10 U C5' C 51.107 . 1 137 . 10 U H5' H 4.573 . 2 138 . 10 U H5'' H 4.213 . 2 139 . 10 U C6 C 140.188 . 1 140 . 10 U H6 H 7.923 . 1 141 . 10 U C5 C 97.353 . 1 142 . 10 U H5 H 5.525 . 1 143 . 11 U C1' C 86.835 . 1 144 . 11 U H1' H 5.709 . 1 145 . 11 U C2' C 65.295 . 1 146 . 11 U H2' H 4.581 . 1 147 . 11 U C3' C 62.934 . 1 148 . 11 U H3' H 4.671 . 1 149 . 11 U C4' C 72.312 . 1 150 . 11 U H4' H 4.519 . 1 151 . 11 U C5' C 51.050 . 1 152 . 11 U H5' H 4.573 . 2 153 . 11 U H5'' H 4.213 . 2 154 . 11 U C6 C 140.055 . 1 155 . 11 U H6 H 7.958 . 1 156 . 11 U C5 C 97.885 . 1 157 . 11 U H5 H 5.681 . 1 158 . 12 G C1' C 86.497 . 1 159 . 12 G H1' H 5.801 . 1 160 . 12 G C2' C 65.130 . 1 161 . 12 G H2' H 4.570 . 1 162 . 12 G C3' C 63.164 . 1 163 . 12 G H3' H 4.598 . 1 164 . 12 G C4' C 72.079 . 1 165 . 12 G H4' H 4.546 . 1 166 . 12 G C5' C 51.705 . 1 167 . 12 G H5' H 4.554 . 2 168 . 12 G H5'' H 4.227 . 2 169 . 12 G C8 C 134.610 . 1 170 . 12 G H8 H 7.831 . 1 171 . 13 C C1' C 87.726 . 1 172 . 13 C H1' H 5.524 . 1 173 . 13 C C2' C 65.065 . 1 174 . 13 C H2' H 4.610 . 1 175 . 13 C C3' C 61.975 . 1 176 . 13 C H3' H 4.347 . 1 177 . 13 C C4' C 71.925 . 1 178 . 13 C H4' H 4.474 . 1 179 . 13 C C5' C 50.442 . 1 180 . 13 C H5' H 4.575 . 2 181 . 13 C H5'' H 4.139 . 2 182 . 13 C C6 C 138.648 . 1 183 . 13 C H6 H 7.563 . 1 184 . 13 C C5 C 90.927 . 1 185 . 13 C H5 H 5.209 . 1 186 . 14 U C1' C 87.891 . 1 187 . 14 U H1' H 5.734 . 1 188 . 14 U C2' C 65.662 . 1 189 . 14 U H2' H 4.338 . 1 190 . 14 U C3' C 63.131 . 1 191 . 14 U H3' H 4.583 . 1 192 . 14 U C4' C 72.278 . 1 193 . 14 U H4' H 4.464 . 1 194 . 14 U C5' C 50.507 . 1 195 . 14 U H5' H 4.513 . 2 196 . 14 U H5'' H 4.136 . 2 197 . 14 U C6 C 138.800 . 1 198 . 14 U H6 H 7.766 . 1 199 . 14 U C5 C 98.886 . 1 200 . 14 U H5 H 5.717 . 1 201 . 15 G C1' C 86.363 . 1 202 . 15 G H1' H 5.782 . 1 203 . 15 G C2' C 65.741 . 1 204 . 15 G H2' H 4.677 . 1 205 . 15 G C3' C 62.824 . 1 206 . 15 G H3' H 4.674 . 1 207 . 15 G C4' C 72.552 . 1 208 . 15 G H4' H 4.486 . 1 209 . 15 G C5' C 51.677 . 1 210 . 15 G H5' H 4.559 . 2 211 . 15 G H5'' H 4.209 . 2 212 . 15 G C8 C 134.878 . 1 213 . 15 G H8 H 7.828 . 1 214 . 16 G C1' C 87.236 . 1 215 . 16 G H1' H 5.715 . 1 216 . 16 G C2' C 65.344 . 1 217 . 16 G H2' H 4.456 . 1 218 . 16 G C3' C 63.071 . 1 219 . 16 G H3' H 4.667 . 1 220 . 16 G C4' C 72.655 . 1 221 . 16 G H4' H 4.438 . 1 222 . 16 G C5' C 51.193 . 1 223 . 16 G H5' H 4.490 . 2 224 . 16 G H5'' H 4.175 . 2 225 . 16 G C8 C 134.381 . 1 226 . 16 G H8 H 7.369 . 1 227 . 17 A C1' C 85.515 . 1 228 . 17 A H1' H 5.830 . 1 229 . 17 A C2' C 65.987 . 1 230 . 17 A H2' H 4.749 . 1 231 . 17 A C3' C 64.205 . 1 232 . 17 A H3' H 4.518 . 1 233 . 17 A C4' C 73.698 . 1 234 . 17 A H4' H 4.296 . 1 235 . 17 A C5' C 51.143 . 1 236 . 17 A H5' H 4.281 . 2 237 . 17 A H5'' H 3.976 . 2 238 . 17 A C8 C 139.772 . 1 239 . 17 A H8 H 8.285 . 1 240 . 17 A C2 C 152.480 . 1 241 . 17 A H2 H 8.021 . 1 242 . 18 G C1' C 85.314 . 1 243 . 18 G H1' H 5.266 . 1 244 . 18 G C2' C 65.514 . 1 245 . 18 G H2' H 4.442 . 1 246 . 18 G C3' C 64.599 . 1 247 . 18 G H3' H 4.667 . 1 248 . 18 G C4' C 73.516 . 1 249 . 18 G H4' H 4.363 . 1 250 . 18 G C5' C 51.883 . 1 251 . 18 G H5' H 4.170 . 2 252 . 18 G H5'' H 4.021 . 2 253 . 18 G C8 C 135.686 . 1 254 . 18 G H8 H 7.686 . 1 255 . 19 A C1' C 89.233 . 1 256 . 19 A H1' H 6.117 . 1 257 . 19 A C2' C 66.532 . 1 258 . 19 A H2' H 4.681 . 1 259 . 19 A C3' C 63.982 . 1 260 . 19 A H3' H 4.936 . 1 261 . 19 A C4' C 73.031 . 1 262 . 19 A H4' H 4.545 . 1 263 . 19 A C5' C 52.076 . 1 264 . 19 A H5' H 4.563 . 2 265 . 19 A H5'' H 4.322 . 2 266 . 19 A C8 C 138.671 . 1 267 . 19 A H8 H 8.283 . 1 268 . 19 A C2 C 153.276 . 1 269 . 19 A H2 H 8.267 . 1 270 . 20 C C1' C 87.479 . 1 271 . 20 C H1' H 4.377 . 1 272 . 20 C C2' C 65.640 . 1 273 . 20 C H2' H 4.550 . 1 274 . 20 C C3' C 64.563 . 1 275 . 20 C H3' H 4.205 . 1 276 . 20 C C4' C 72.352 . 1 277 . 20 C H4' H 4.311 . 1 278 . 20 C C5' C 58.023 . 1 279 . 20 C H5' H 4.322 . 2 280 . 20 C H5'' H 4.320 . 2 281 . 20 C C6 C 139.609 . 1 282 . 20 C H6 H 7.843 . 1 283 . 20 C C5 C 93.163 . 1 284 . 20 C H5 H 6.073 . 1 285 . 21 G C1' C 87.503 . 1 286 . 21 G H1' H 5.788 . 1 287 . 21 G C2' C 64.735 . 1 288 . 21 G H2' H 4.837 . 1 289 . 21 G C3' C 64.023 . 1 290 . 21 G H3' H 4.311 . 1 291 . 21 G C4' C 72.626 . 1 292 . 21 G H4' H 4.533 . 1 293 . 21 G C5' C 58.117 . 1 294 . 21 G H5' H 4.349 . 2 295 . 21 G H5'' H 4.127 . 2 296 . 21 G C8 C 135.686 . 1 297 . 21 G H8 H 7.658 . 1 298 . 22 G C1' C 86.906 . 1 299 . 22 G H1' H 5.771 . 1 300 . 22 G C2' C 65.242 . 1 301 . 22 G H2' H 4.581 . 1 302 . 22 G C3' C 62.408 . 1 303 . 22 G H3' H 4.569 . 1 304 . 22 G C4' C 72.181 . 1 305 . 22 G H4' H 4.517 . 1 306 . 22 G C5' C 50.360 . 1 307 . 22 G H5' H 4.545 . 2 308 . 22 G H5'' H 4.128 . 2 309 . 22 G C8 C 134.066 . 1 310 . 22 G H8 H 7.288 . 1 311 . 23 C C1' C 87.792 . 1 312 . 23 C H1' H 5.549 . 1 313 . 23 C C2' C 65.225 . 1 314 . 23 C H2' H 4.616 . 1 315 . 23 C C3' C 62.296 . 1 316 . 23 C H3' H 4.534 . 1 317 . 23 C C4' C 72.050 . 1 318 . 23 C H4' H 4.522 . 1 319 . 23 C C5' C 50.261 . 1 320 . 23 C H5' H 4.577 . 2 321 . 23 C H5'' H 4.135 . 2 322 . 23 C C6 C 138.422 . 1 323 . 23 C H6 H 7.552 . 1 324 . 23 C C5 C 91.357 . 1 325 . 23 C H5 H 5.276 . 1 326 . 24 A C1' C 86.866 . 1 327 . 24 A H1' H 5.934 . 1 328 . 24 A C2' C 65.579 . 1 329 . 24 A H2' H 4.620 . 1 330 . 24 A C3' C 63.082 . 1 331 . 24 A H3' H 4.676 . 1 332 . 24 A C4' C 72.153 . 1 333 . 24 A H4' H 4.554 . 1 334 . 24 A C5' C 51.656 . 1 335 . 24 A H5' H 4.580 . 2 336 . 24 A H5'' H 4.207 . 2 337 . 24 A C8 C 137.441 . 1 338 . 24 A H8 H 7.967 . 1 339 . 24 A C2 C 150.090 . 1 340 . 24 A H2 H 6.764 . 1 341 . 25 A C1' C 86.205 . 1 342 . 25 A H1' H 5.809 . 1 343 . 25 A C2' C 65.673 . 1 344 . 25 A H2' H 4.523 . 1 345 . 25 A C3' C 62.994 . 1 346 . 25 A H3' H 4.615 . 1 347 . 25 A C4' C 72.090 . 1 348 . 25 A H4' H 4.510 . 1 349 . 25 A C5' C 51.592 . 1 350 . 25 A H5' H 4.545 . 2 351 . 25 A H5'' H 4.189 . 2 352 . 25 A C8 C 137.089 . 1 353 . 25 A H8 H 7.702 . 1 354 . 25 A C2 C 151.152 . 1 355 . 25 A H2 H 7.487 . 1 356 . 26 G C1' C 85.990 . 1 357 . 26 G H1' H 5.507 . 1 358 . 26 G C2' C 65.273 . 1 359 . 26 G H2' H 4.400 . 1 360 . 26 G C3' C 63.668 . 1 361 . 26 G H3' H 4.499 . 1 362 . 26 G C4' C 72.620 . 1 363 . 26 G H4' H 4.446 . 1 364 . 26 G C5' C 51.947 . 1 365 . 26 G H5' H 4.441 . 2 366 . 26 G H5'' H 4.113 . 2 367 . 26 G C8 C 134.509 . 1 368 . 26 G H8 H 7.176 . 1 369 . 27 A C1' C 85.771 . 1 370 . 27 A H1' H 5.878 . 1 371 . 27 A C2' C 65.859 . 1 372 . 27 A H2' H 4.435 . 1 373 . 27 A C3' C 64.125 . 1 374 . 27 A H3' H 4.614 . 1 375 . 27 A C4' C 73.356 . 1 376 . 27 A H4' H 4.467 . 1 377 . 27 A C5' C 52.147 . 1 378 . 27 A H5' H 4.437 . 2 379 . 27 A H5'' H 4.131 . 2 380 . 27 A C8 C 137.928 . 1 381 . 27 A H8 H 7.830 . 1 382 . 27 A C2 C 151.983 . 1 383 . 27 A H2 H 7.712 . 1 384 . 28 G C1' C 84.651 . 1 385 . 28 G H1' H 5.594 . 1 386 . 28 G C2' C 65.121 . 1 387 . 28 G H2' H 4.593 . 1 388 . 28 G C3' C 65.432 . 1 389 . 28 G H3' H 4.636 . 1 390 . 28 G C4' C 74.217 . 1 391 . 28 G H4' H 4.435 . 1 392 . 28 G C5' C 52.759 . 1 393 . 28 G H5' H 4.316 . 2 394 . 28 G H5'' H 4.129 . 2 395 . 28 G C8 C 136.613 . 1 396 . 28 G H8 H 7.560 . 1 397 . 29 G C1' C 84.004 . 1 398 . 29 G H1' H 5.809 . 1 399 . 29 G C2' C 65.201 . 1 400 . 29 G H2' H 4.791 . 1 401 . 29 G C3' C 66.367 . 1 402 . 29 G H3' H 4.798 . 1 403 . 29 G C4' C 74.507 . 1 404 . 29 G H4' H 4.593 . 1 405 . 29 G C5' C 51.144 . 1 406 . 29 G H5' H 4.646 . 2 407 . 29 G H5'' H 4.239 . 2 408 . 29 G C8 C 137.091 . 1 409 . 29 G H8 H 7.875 . 1 410 . 30 C C1' C 87.428 . 1 411 . 30 C H1' H 5.745 . 1 412 . 30 C C2' C 65.229 . 1 413 . 30 C H2' H 4.659 . 1 414 . 30 C C3' C 65.234 . 1 415 . 30 C H3' H 4.735 . 1 416 . 30 C C4' C 72.615 . 1 417 . 30 C H4' H 4.554 . 1 418 . 30 C C5' C 52.645 . 1 419 . 30 C H5' H 4.454 . 2 420 . 30 C H5'' H 4.318 . 2 421 . 30 C C6 C 140.118 . 1 422 . 30 C H6 H 7.791 . 1 423 . 30 C C5 C 92.434 . 1 424 . 30 C H5 H 5.674 . 1 425 . 31 G C1' C 86.567 . 1 426 . 31 G H1' H 5.807 . 1 427 . 31 G C2' C 65.442 . 1 428 . 31 G H2' H 4.753 . 1 429 . 31 G C3' C 65.607 . 1 430 . 31 G H3' H 4.682 . 1 431 . 31 G C4' C 72.421 . 1 432 . 31 G H4' H 4.571 . 1 433 . 31 G C5' C 52.332 . 1 434 . 31 G H5' H 4.544 . 2 435 . 31 G H5'' H 4.288 . 2 436 . 31 G C8 C 134.526 . 1 437 . 31 G H8 H 7.713 . 1 438 . 32 A C1' C 86.697 . 1 439 . 32 A H1' H 6.011 . 1 440 . 32 A C2' C 65.674 . 1 441 . 32 A H2' H 4.777 . 1 442 . 32 A C3' C 62.840 . 1 443 . 32 A H3' H 4.735 . 1 444 . 32 A C4' C 71.993 . 1 445 . 32 A H4' H 4.575 . 1 446 . 32 A C5' C 51.172 . 1 447 . 32 A H5' H 4.638 . 2 448 . 32 A H5'' H 4.237 . 2 449 . 32 A C8 C 137.174 . 1 450 . 32 A H8 H 7.829 . 1 451 . 32 A C2 C 151.147 . 1 452 . 32 A H2 H 7.503 . 1 453 . 33 G C1' C 86.801 . 1 454 . 33 G H1' H 5.638 . 1 455 . 33 G C2' C 65.267 . 1 456 . 33 G H2' H 4.493 . 1 457 . 33 G C3' C 63.139 . 1 458 . 33 G H3' H 4.408 . 1 459 . 33 G C4' C 72.113 . 1 460 . 33 G H4' H 4.531 . 1 461 . 33 G C5' C 52.033 . 1 462 . 33 G H5' H 4.493 . 2 463 . 33 G H5'' H 4.121 . 2 464 . 33 G C8 C 133.663 . 1 465 . 33 G H8 H 7.144 . 1 466 . 34 U C1' C 87.608 . 1 467 . 34 U H1' H 5.637 . 1 468 . 34 U C2' C 65.212 . 1 469 . 34 U H2' H 4.576 . 1 470 . 34 U C3' C 62.261 . 1 471 . 34 U H3' H 4.548 . 1 472 . 34 U C4' C 72.084 . 1 473 . 34 U H4' H 4.500 . 1 474 . 34 U C5' C 51.115 . 1 475 . 34 U H5' H 4.572 . 2 476 . 34 U H5'' H 4.200 . 2 477 . 34 U C6 C 139.591 . 1 478 . 34 U H6 H 7.787 . 1 479 . 34 U C5 C 96.417 . 1 480 . 34 U H5 H 5.096 . 1 481 . 35 C C1' C 88.205 . 1 482 . 35 C H1' H 5.690 . 1 483 . 35 C C2' C 65.802 . 1 484 . 35 C H2' H 4.335 . 1 485 . 35 C C3' C 62.461 . 1 486 . 35 C H3' H 4.568 . 1 487 . 35 C C4' C 72.022 . 1 488 . 35 C H4' H 4.503 . 1 489 . 35 C C5' C 50.830 . 1 490 . 35 C H5' H 4.606 . 2 491 . 35 C H5'' H 4.185 . 2 492 . 35 C C6 C 139.640 . 1 493 . 35 C H6 H 7.936 . 1 494 . 35 C C5 C 91.472 . 1 495 . 35 C H5 H 5.726 . 1 496 . 36 C C1' C 86.657 . 1 497 . 36 C H1' H 5.807 . 1 498 . 36 C C2' C 67.472 . 1 499 . 36 C H2' H 4.064 . 1 500 . 36 C C3' C 59.843 . 1 501 . 36 C H3' H 4.247 . 1 502 . 36 C C4' C 73.522 . 1 503 . 36 C H4' H 4.241 . 1 504 . 36 C C5' C 58.134 . 1 505 . 36 C H5' H 4.347 . 2 506 . 36 C H5'' H 4.165 . 2 507 . 36 C C6 C 139.754 . 1 508 . 36 C H6 H 7.754 . 1 509 . 36 C C5 C 92.109 . 1 stop_ save_