data_25553 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 25553 _Entry.Title ; Docked structure between SUMO1 and ZZ-domain from CBP ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2015-03-26 _Entry.Accession_date 2016-03-23 _Entry.Last_release_date 2016-06-01 _Entry.Original_release_date 2016-06-01 _Entry.Origination author _Entry.NMR_STAR_version 3.1.2.6 _Entry.Original_NMR_STAR_version 3.1.1.99 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype 'SOLUTION NMR' _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 C. Diehl . . . . 25553 stop_ loop_ _SG_project.SG_project_ID _SG_project.Project_name _SG_project.Full_name_of_center _SG_project.Initial_of_center _SG_project.Entry_ID 1 'not applicable' 'not applicable' . 25553 stop_ loop_ _Struct_keywords.Keywords _Struct_keywords.Text _Struct_keywords.Entry_ID SIM . 25553 SUMO1 . 25553 ZZ-domain . 25553 'docked structure' . 25553 'protein-protein complex' . 25553 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 25553 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 14 25553 '15N chemical shifts' 92 25553 '1H chemical shifts' 92 25553 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 2 . . 2016-08-25 2015-03-26 update BMRB 'update entry citation' 25553 1 . . 2016-06-01 2015-03-26 original author 'original release' 25553 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID PDB 2N1A 'BMRB Entry Tracking System' 25553 stop_ save_ ############### # Citations # ############### save_citations _Citation.Sf_category citations _Citation.Sf_framecode citations _Citation.Entry_ID 25553 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 27129204 _Citation.Full_citation . _Citation.Title ; Structural Analysis of a Complex between Small Ubiquitin-like Modifier 1 (SUMO1) and the ZZ Domain of CREB-binding Protein (CBP/p300) Reveals a New Interaction Surface on SUMO ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'J. Biol. Chem.' _Citation.Journal_name_full . _Citation.Journal_volume 291 _Citation.Journal_issue 24 _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 12658 _Citation.Page_last 12672 _Citation.Year 2016 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 C. Diehl . . . . 25553 1 2 M. Akke . . . . 25553 1 3 S. Bekker-Jensen . . . . 25553 1 4 N. Mailand . . . . 25553 1 5 W. Streicher . . . . 25553 1 6 M. Wikstrom . . . . 25553 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 25553 _Assembly.ID 1 _Assembly.Name 'SUMO1 and ZZ-domain from CBP' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds . _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 entity_1 1 $entity_1 A . yes native no no . . . 25553 1 2 entity_2 2 $entity_2 B . no native no no . . . 25553 1 3 ZN_1 3 $entity_ZN C . no native no no . . . 25553 1 4 ZN_2 3 $entity_ZN D . no native no no . . . 25553 1 stop_ loop_ _Bond.ID _Bond.Type _Bond.Value_order _Bond.Assembly_atom_ID_1 _Bond.Entity_assembly_ID_1 _Bond.Entity_assembly_name_1 _Bond.Entity_ID_1 _Bond.Comp_ID_1 _Bond.Comp_index_ID_1 _Bond.Seq_ID_1 _Bond.Atom_ID_1 _Bond.Assembly_atom_ID_2 _Bond.Entity_assembly_ID_2 _Bond.Entity_assembly_name_2 _Bond.Entity_ID_2 _Bond.Comp_ID_2 _Bond.Comp_index_ID_2 _Bond.Seq_ID_2 _Bond.Atom_ID_2 _Bond.Auth_entity_assembly_ID_1 _Bond.Auth_entity_assembly_name_1 _Bond.Auth_seq_ID_1 _Bond.Auth_comp_ID_1 _Bond.Auth_atom_ID_1 _Bond.Auth_entity_assembly_ID_2 _Bond.Auth_entity_assembly_name_2 _Bond.Auth_seq_ID_2 _Bond.Auth_comp_ID_2 _Bond.Auth_atom_ID_2 _Bond.Entry_ID _Bond.Assembly_ID 1 coordinate single . 2 entity_2 2 CYS 9 9 SG . 3 ZN_1 3 ZN 1 1 ZN . . 209 CYS . . . 300 ZN . 25553 1 2 coordinate single . 2 entity_2 2 CYS 12 12 SG . 3 ZN_1 3 ZN 1 1 ZN . . 212 CYS . . . 300 ZN . 25553 1 3 coordinate single . 2 entity_2 2 CYS 22 22 SG . 4 ZN_2 3 ZN 1 1 ZN . . 222 CYS . . . 301 ZN . 25553 1 4 coordinate single . 2 entity_2 2 CYS 25 25 SG . 4 ZN_2 3 ZN 1 1 ZN . . 225 CYS . . . 301 ZN . 25553 1 5 coordinate single . 2 entity_2 2 CYS 31 31 SG . 3 ZN_1 3 ZN 1 1 ZN . . 231 CYS . . . 300 ZN . 25553 1 6 coordinate single . 2 entity_2 2 CYS 34 34 SG . 3 ZN_1 3 ZN 1 1 ZN . . 234 CYS . . . 300 ZN . 25553 1 7 coordinate single . 2 entity_2 2 HIS 40 40 NE2 . 4 ZN_2 3 ZN 1 1 ZN . . 240 HIS . . . 301 ZN . 25553 1 8 coordinate single . 2 entity_2 2 HIS 42 42 NE2 . 4 ZN_2 3 ZN 1 1 ZN . . 242 HIS . . . 301 ZN . 25553 1 stop_ loop_ _Entity_deleted_atom.ID _Entity_deleted_atom.Entity_atom_list_ID _Entity_deleted_atom.Entity_assembly_ID _Entity_deleted_atom.Entity_ID _Entity_deleted_atom.Comp_ID _Entity_deleted_atom.Comp_index_ID _Entity_deleted_atom.Seq_ID _Entity_deleted_atom.Atom_ID _Entity_deleted_atom.Auth_entity_assembly_ID _Entity_deleted_atom.Auth_seq_ID _Entity_deleted_atom.Auth_comp_ID _Entity_deleted_atom.Auth_atom_ID _Entity_deleted_atom.Entry_ID _Entity_deleted_atom.Assembly_ID 1 . 2 2 CYS 9 9 HG . 209 . . 25553 1 2 . 2 2 CYS 12 12 HG . 212 . . 25553 1 3 . 2 2 CYS 22 22 HG . 222 . . 25553 1 4 . 2 2 CYS 25 25 HG . 225 . . 25553 1 5 . 2 2 CYS 31 31 HG . 231 . . 25553 1 6 . 2 2 CYS 34 34 HG . 234 . . 25553 1 7 . 2 2 HIS 40 40 HE2 . 240 . . 25553 1 8 . 2 2 HIS 42 42 HD1 . 242 . . 25553 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 25553 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID A _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MSDQEAKPSTEDLGDKKEGE YIKLKVIGQDSSEIHFKVKM TTHLKKLKESYCQRQGVPMN SLRFLFEGQRIADNHTPKEL GMEEEDVIEVYQEQTGHSTV ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 100 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method man _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight 11518.051 _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 1 MET . 25553 1 2 2 SER . 25553 1 3 3 ASP . 25553 1 4 4 GLN . 25553 1 5 5 GLU . 25553 1 6 6 ALA . 25553 1 7 7 LYS . 25553 1 8 8 PRO . 25553 1 9 9 SER . 25553 1 10 10 THR . 25553 1 11 11 GLU . 25553 1 12 12 ASP . 25553 1 13 13 LEU . 25553 1 14 14 GLY . 25553 1 15 15 ASP . 25553 1 16 16 LYS . 25553 1 17 17 LYS . 25553 1 18 18 GLU . 25553 1 19 19 GLY . 25553 1 20 20 GLU . 25553 1 21 21 TYR . 25553 1 22 22 ILE . 25553 1 23 23 LYS . 25553 1 24 24 LEU . 25553 1 25 25 LYS . 25553 1 26 26 VAL . 25553 1 27 27 ILE . 25553 1 28 28 GLY . 25553 1 29 29 GLN . 25553 1 30 30 ASP . 25553 1 31 31 SER . 25553 1 32 32 SER . 25553 1 33 33 GLU . 25553 1 34 34 ILE . 25553 1 35 35 HIS . 25553 1 36 36 PHE . 25553 1 37 37 LYS . 25553 1 38 38 VAL . 25553 1 39 39 LYS . 25553 1 40 40 MET . 25553 1 41 41 THR . 25553 1 42 42 THR . 25553 1 43 43 HIS . 25553 1 44 44 LEU . 25553 1 45 45 LYS . 25553 1 46 46 LYS . 25553 1 47 47 LEU . 25553 1 48 48 LYS . 25553 1 49 49 GLU . 25553 1 50 50 SER . 25553 1 51 51 TYR . 25553 1 52 52 CYS . 25553 1 53 53 GLN . 25553 1 54 54 ARG . 25553 1 55 55 GLN . 25553 1 56 56 GLY . 25553 1 57 57 VAL . 25553 1 58 58 PRO . 25553 1 59 59 MET . 25553 1 60 60 ASN . 25553 1 61 61 SER . 25553 1 62 62 LEU . 25553 1 63 63 ARG . 25553 1 64 64 PHE . 25553 1 65 65 LEU . 25553 1 66 66 PHE . 25553 1 67 67 GLU . 25553 1 68 68 GLY . 25553 1 69 69 GLN . 25553 1 70 70 ARG . 25553 1 71 71 ILE . 25553 1 72 72 ALA . 25553 1 73 73 ASP . 25553 1 74 74 ASN . 25553 1 75 75 HIS . 25553 1 76 76 THR . 25553 1 77 77 PRO . 25553 1 78 78 LYS . 25553 1 79 79 GLU . 25553 1 80 80 LEU . 25553 1 81 81 GLY . 25553 1 82 82 MET . 25553 1 83 83 GLU . 25553 1 84 84 GLU . 25553 1 85 85 GLU . 25553 1 86 86 ASP . 25553 1 87 87 VAL . 25553 1 88 88 ILE . 25553 1 89 89 GLU . 25553 1 90 90 VAL . 25553 1 91 91 TYR . 25553 1 92 92 GLN . 25553 1 93 93 GLU . 25553 1 94 94 GLN . 25553 1 95 95 THR . 25553 1 96 96 GLY . 25553 1 97 97 HIS . 25553 1 98 98 SER . 25553 1 99 99 THR . 25553 1 100 100 VAL . 25553 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 25553 1 . SER 2 2 25553 1 . ASP 3 3 25553 1 . GLN 4 4 25553 1 . GLU 5 5 25553 1 . ALA 6 6 25553 1 . LYS 7 7 25553 1 . PRO 8 8 25553 1 . SER 9 9 25553 1 . THR 10 10 25553 1 . GLU 11 11 25553 1 . ASP 12 12 25553 1 . LEU 13 13 25553 1 . GLY 14 14 25553 1 . ASP 15 15 25553 1 . LYS 16 16 25553 1 . LYS 17 17 25553 1 . GLU 18 18 25553 1 . GLY 19 19 25553 1 . GLU 20 20 25553 1 . TYR 21 21 25553 1 . ILE 22 22 25553 1 . LYS 23 23 25553 1 . LEU 24 24 25553 1 . LYS 25 25 25553 1 . VAL 26 26 25553 1 . ILE 27 27 25553 1 . GLY 28 28 25553 1 . GLN 29 29 25553 1 . ASP 30 30 25553 1 . SER 31 31 25553 1 . SER 32 32 25553 1 . GLU 33 33 25553 1 . ILE 34 34 25553 1 . HIS 35 35 25553 1 . PHE 36 36 25553 1 . LYS 37 37 25553 1 . VAL 38 38 25553 1 . LYS 39 39 25553 1 . MET 40 40 25553 1 . THR 41 41 25553 1 . THR 42 42 25553 1 . HIS 43 43 25553 1 . LEU 44 44 25553 1 . LYS 45 45 25553 1 . LYS 46 46 25553 1 . LEU 47 47 25553 1 . LYS 48 48 25553 1 . GLU 49 49 25553 1 . SER 50 50 25553 1 . TYR 51 51 25553 1 . CYS 52 52 25553 1 . GLN 53 53 25553 1 . ARG 54 54 25553 1 . GLN 55 55 25553 1 . GLY 56 56 25553 1 . VAL 57 57 25553 1 . PRO 58 58 25553 1 . MET 59 59 25553 1 . ASN 60 60 25553 1 . SER 61 61 25553 1 . LEU 62 62 25553 1 . ARG 63 63 25553 1 . PHE 64 64 25553 1 . LEU 65 65 25553 1 . PHE 66 66 25553 1 . GLU 67 67 25553 1 . GLY 68 68 25553 1 . GLN 69 69 25553 1 . ARG 70 70 25553 1 . ILE 71 71 25553 1 . ALA 72 72 25553 1 . ASP 73 73 25553 1 . ASN 74 74 25553 1 . HIS 75 75 25553 1 . THR 76 76 25553 1 . PRO 77 77 25553 1 . LYS 78 78 25553 1 . GLU 79 79 25553 1 . LEU 80 80 25553 1 . GLY 81 81 25553 1 . MET 82 82 25553 1 . GLU 83 83 25553 1 . GLU 84 84 25553 1 . GLU 85 85 25553 1 . ASP 86 86 25553 1 . VAL 87 87 25553 1 . ILE 88 88 25553 1 . GLU 89 89 25553 1 . VAL 90 90 25553 1 . TYR 91 91 25553 1 . GLN 92 92 25553 1 . GLU 93 93 25553 1 . GLN 94 94 25553 1 . THR 95 95 25553 1 . GLY 96 96 25553 1 . HIS 97 97 25553 1 . SER 98 98 25553 1 . THR 99 99 25553 1 . VAL 100 100 25553 1 stop_ save_ save_entity_2 _Entity.Sf_category entity _Entity.Sf_framecode entity_2 _Entity.Entry_ID 25553 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name entity_2 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID B _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GQDRFVYTCNECKHHVETRW HCTVCEDYDLCINCYNTKSH AHKMVKWGLGLDD ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 53 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all other bound' _Entity.Src_method man _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight 6315.152 _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 201 GLY . 25553 2 2 202 GLN . 25553 2 3 203 ASP . 25553 2 4 204 ARG . 25553 2 5 205 PHE . 25553 2 6 206 VAL . 25553 2 7 207 TYR . 25553 2 8 208 THR . 25553 2 9 209 CYS . 25553 2 10 210 ASN . 25553 2 11 211 GLU . 25553 2 12 212 CYS . 25553 2 13 213 LYS . 25553 2 14 214 HIS . 25553 2 15 215 HIS . 25553 2 16 216 VAL . 25553 2 17 217 GLU . 25553 2 18 218 THR . 25553 2 19 219 ARG . 25553 2 20 220 TRP . 25553 2 21 221 HIS . 25553 2 22 222 CYS . 25553 2 23 223 THR . 25553 2 24 224 VAL . 25553 2 25 225 CYS . 25553 2 26 226 GLU . 25553 2 27 227 ASP . 25553 2 28 228 TYR . 25553 2 29 229 ASP . 25553 2 30 230 LEU . 25553 2 31 231 CYS . 25553 2 32 232 ILE . 25553 2 33 233 ASN . 25553 2 34 234 CYS . 25553 2 35 235 TYR . 25553 2 36 236 ASN . 25553 2 37 237 THR . 25553 2 38 238 LYS . 25553 2 39 239 SER . 25553 2 40 240 HIS . 25553 2 41 241 ALA . 25553 2 42 242 HIS . 25553 2 43 243 LYS . 25553 2 44 244 MET . 25553 2 45 245 VAL . 25553 2 46 246 LYS . 25553 2 47 247 TRP . 25553 2 48 248 GLY . 25553 2 49 249 LEU . 25553 2 50 250 GLY . 25553 2 51 251 LEU . 25553 2 52 252 ASP . 25553 2 53 253 ASP . 25553 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 25553 2 . GLN 2 2 25553 2 . ASP 3 3 25553 2 . ARG 4 4 25553 2 . PHE 5 5 25553 2 . VAL 6 6 25553 2 . TYR 7 7 25553 2 . THR 8 8 25553 2 . CYS 9 9 25553 2 . ASN 10 10 25553 2 . GLU 11 11 25553 2 . CYS 12 12 25553 2 . LYS 13 13 25553 2 . HIS 14 14 25553 2 . HIS 15 15 25553 2 . VAL 16 16 25553 2 . GLU 17 17 25553 2 . THR 18 18 25553 2 . ARG 19 19 25553 2 . TRP 20 20 25553 2 . HIS 21 21 25553 2 . CYS 22 22 25553 2 . THR 23 23 25553 2 . VAL 24 24 25553 2 . CYS 25 25 25553 2 . GLU 26 26 25553 2 . ASP 27 27 25553 2 . TYR 28 28 25553 2 . ASP 29 29 25553 2 . LEU 30 30 25553 2 . CYS 31 31 25553 2 . ILE 32 32 25553 2 . ASN 33 33 25553 2 . CYS 34 34 25553 2 . TYR 35 35 25553 2 . ASN 36 36 25553 2 . THR 37 37 25553 2 . LYS 38 38 25553 2 . SER 39 39 25553 2 . HIS 40 40 25553 2 . ALA 41 41 25553 2 . HIS 42 42 25553 2 . LYS 43 43 25553 2 . MET 44 44 25553 2 . VAL 45 45 25553 2 . LYS 46 46 25553 2 . TRP 47 47 25553 2 . GLY 48 48 25553 2 . LEU 49 49 25553 2 . GLY 50 50 25553 2 . LEU 51 51 25553 2 . ASP 52 52 25553 2 . ASP 53 53 25553 2 stop_ save_ save_entity_ZN _Entity.Sf_category entity _Entity.Sf_framecode entity_ZN _Entity.Entry_ID 25553 _Entity.ID 3 _Entity.BMRB_code ZN _Entity.Name 'ZINC ION' _Entity.Type non-polymer _Entity.Polymer_common_type . _Entity.Polymer_type . _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code . _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states . _Entity.Ambiguous_chem_comp_sites . _Entity.Nstd_monomer . _Entity.Nstd_chirality . _Entity.Nstd_linkage . _Entity.Nonpolymer_comp_ID ZN _Entity.Nonpolymer_comp_label $chem_comp_ZN _Entity.Number_of_monomers . _Entity.Number_of_nonpolymer_components 1 _Entity.Paramagnetic . _Entity.Thiol_state . _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight 65.409 _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_common_name.Name _Entity_common_name.Type _Entity_common_name.Entry_ID _Entity_common_name.Entity_ID 'ZINC ION' BMRB 25553 3 stop_ loop_ _Entity_systematic_name.Name _Entity_systematic_name.Naming_system _Entity_systematic_name.Entry_ID _Entity_systematic_name.Entity_ID 'ZINC ION' BMRB 25553 3 ZN 'Three letter code' 25553 3 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 1 ZN $chem_comp_ZN 25553 3 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 25553 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 25553 1 2 2 $entity_2 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . 25553 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 25553 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . 469008 Escherichia coli 'DE3 Rosetta' . . . . . pNIC28 . . . 25553 1 2 2 $entity_2 . 'recombinant technology' 'Escherichia coli' . . 469008 Escherichia coli 'DE3 Rosetta' . . . . . pNIC28 . . . 25553 1 stop_ save_ ################################# # Polymer residues and ligands # ################################# save_chem_comp_ZN _Chem_comp.Sf_category chem_comp _Chem_comp.Sf_framecode chem_comp_ZN _Chem_comp.Entry_ID 25553 _Chem_comp.ID ZN _Chem_comp.Provenance PDB _Chem_comp.Name 'ZINC ION' _Chem_comp.Type NON-POLYMER _Chem_comp.BMRB_code ZN _Chem_comp.PDB_code ZN _Chem_comp.Ambiguous_flag no _Chem_comp.Initial_date 2012-11-20 _Chem_comp.Modified_date 2012-11-20 _Chem_comp.Release_status REL _Chem_comp.Replaced_by . _Chem_comp.Replaces . _Chem_comp.One_letter_code . _Chem_comp.Three_letter_code ZN _Chem_comp.Number_atoms_all 1 _Chem_comp.Number_atoms_nh 1 _Chem_comp.PubChem_code . _Chem_comp.Subcomponent_list . _Chem_comp.InChI_code InChI=1S/Zn/q+2 _Chem_comp.Mon_nstd_flag . _Chem_comp.Mon_nstd_class . _Chem_comp.Mon_nstd_details . _Chem_comp.Mon_nstd_parent . _Chem_comp.Mon_nstd_parent_comp_ID . _Chem_comp.Std_deriv_one_letter_code . _Chem_comp.Std_deriv_three_letter_code . _Chem_comp.Std_deriv_BMRB_code . _Chem_comp.Std_deriv_PDB_code . _Chem_comp.Std_deriv_chem_comp_name . _Chem_comp.Synonyms . _Chem_comp.Formal_charge 2 _Chem_comp.Paramagnetic . _Chem_comp.Aromatic no _Chem_comp.Formula Zn _Chem_comp.Formula_weight 65.409 _Chem_comp.Formula_mono_iso_wt_nat . _Chem_comp.Formula_mono_iso_wt_13C . _Chem_comp.Formula_mono_iso_wt_15N . _Chem_comp.Formula_mono_iso_wt_13C_15N . _Chem_comp.Image_file_name . _Chem_comp.Image_file_format . _Chem_comp.Topo_file_name . _Chem_comp.Topo_file_format . _Chem_comp.Struct_file_name . _Chem_comp.Struct_file_format . _Chem_comp.Stereochem_param_file_name . _Chem_comp.Stereochem_param_file_format . _Chem_comp.Model_details . _Chem_comp.Model_erf . _Chem_comp.Model_source . _Chem_comp.Model_coordinates_details . _Chem_comp.Model_coordinates_missing_flag no _Chem_comp.Ideal_coordinates_details . _Chem_comp.Ideal_coordinates_missing_flag no _Chem_comp.Model_coordinates_db_code . _Chem_comp.Processing_site RCSB _Chem_comp.Vendor . _Chem_comp.Vendor_product_code . _Chem_comp.Details . _Chem_comp.DB_query_date . _Chem_comp.DB_last_query_revised_last_date . loop_ _Chem_comp_descriptor.Descriptor _Chem_comp_descriptor.Type _Chem_comp_descriptor.Program _Chem_comp_descriptor.Program_version _Chem_comp_descriptor.Entry_ID _Chem_comp_descriptor.Comp_ID InChI=1S/Zn/q+2 InChI InChI 1.03 25553 ZN PTFCDOFLOPIGGS-UHFFFAOYSA-N InChIKey InChI 1.03 25553 ZN [Zn++] SMILES CACTVS 3.341 25553 ZN [Zn++] SMILES_CANONICAL CACTVS 3.341 25553 ZN [Zn+2] SMILES ACDLabs 10.04 25553 ZN [Zn+2] SMILES 'OpenEye OEToolkits' 1.5.0 25553 ZN [Zn+2] SMILES_CANONICAL 'OpenEye OEToolkits' 1.5.0 25553 ZN stop_ loop_ _Chem_comp_identifier.Identifier _Chem_comp_identifier.Type _Chem_comp_identifier.Program _Chem_comp_identifier.Program_version _Chem_comp_identifier.Entry_ID _Chem_comp_identifier.Comp_ID zinc 'SYSTEMATIC NAME' ACDLabs 10.04 25553 ZN 'zinc(+2) cation' 'SYSTEMATIC NAME' 'OpenEye OEToolkits' 1.5.0 25553 ZN stop_ loop_ _Chem_comp_atom.Atom_ID _Chem_comp_atom.BMRB_code _Chem_comp_atom.PDB_atom_ID _Chem_comp_atom.Alt_atom_ID _Chem_comp_atom.Auth_atom_ID _Chem_comp_atom.Type_symbol _Chem_comp_atom.Isotope_number _Chem_comp_atom.Chirality _Chem_comp_atom.Stereo_config _Chem_comp_atom.Charge _Chem_comp_atom.Partial_charge _Chem_comp_atom.Oxidation_number _Chem_comp_atom.Unpaired_electron_number _Chem_comp_atom.Align _Chem_comp_atom.Aromatic_flag _Chem_comp_atom.Leaving_atom_flag _Chem_comp_atom.Substruct_code _Chem_comp_atom.Ionizable _Chem_comp_atom.Drawing_2D_coord_x _Chem_comp_atom.Drawing_2D_coord_y _Chem_comp_atom.Model_Cartn_x _Chem_comp_atom.Model_Cartn_x_esd _Chem_comp_atom.Model_Cartn_y _Chem_comp_atom.Model_Cartn_y_esd _Chem_comp_atom.Model_Cartn_z _Chem_comp_atom.Model_Cartn_z_esd _Chem_comp_atom.Model_Cartn_x_ideal _Chem_comp_atom.Model_Cartn_y_ideal _Chem_comp_atom.Model_Cartn_z_ideal _Chem_comp_atom.PDBX_ordinal _Chem_comp_atom.Details _Chem_comp_atom.Entry_ID _Chem_comp_atom.Comp_ID ZN ZN ZN ZN . ZN . . N 2 . . . 0 no no . . . . 0.000 . 0.000 . 0.000 . 0.000 0.000 0.000 1 . 25553 ZN stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 25553 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details '20 mM MES pH 6.0, 90% H2O, 10% D2O' _Sample.Aggregate_sample_number . _Sample.Solvent_system '93% H2O/7% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 entity_1 . . . 1 $entity_1 . protein . . . mM . . . . 25553 1 2 entity_2 . . . 2 $entity_2 . protein . . . mM . . . . 25553 1 3 MES 'natural abundance' . . . . . buffer 20 . . mM . . . . 25553 1 4 H2O 'natural abundance' . . . . . solvent 93 . . % . . . . 25553 1 5 D2O [U-2H] . . . . . solvent 7 . . % . . . . 25553 1 stop_ save_ save_sample_2 _Sample.Sf_category sample _Sample.Sf_framecode sample_2 _Sample.Entry_ID 25553 _Sample.ID 2 _Sample.Type solution _Sample.Sub_type . _Sample.Details '20 mM MES pH 6.0, 90% H2O, 10% D2O' _Sample.Aggregate_sample_number . _Sample.Solvent_system '90% H2O, 10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 entity_1 . . . 1 $entity_1 . protein . . . mM . . . . 25553 2 2 entity_2 . . . 2 $entity_2 . protein . . . mM . . . . 25553 2 3 MES 'natural abundance' . . . . . buffer 20 . . mM . . . . 25553 2 4 H2O 'natural abundance' . . . . . solvent 90 . . % . . . . 25553 2 5 D2O [U-2H] . . . . . solvent 10 . . % . . . . 25553 2 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 25553 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 6 . pH 25553 1 pressure 1 . atm 25553 1 temperature 298 . K 25553 1 stop_ save_ ############################ # Computer software used # ############################ save_CNS _Software.Sf_category software _Software.Sf_framecode CNS _Software.Entry_ID 25553 _Software.ID 1 _Software.Name CNS _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Brunger A. T. et.al.' . . 25553 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID refinement 25553 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 25553 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model INOVA _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 25553 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Varian INOVA . 600 . . . 25553 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 25553 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '15N IPAP-HSQC' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 25553 1 2 '15N IPAP-HSQC' no . . . . . . . . . . 2 $sample_2 anisotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 25553 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chemical_shift_reference_1 _Chem_shift_reference.Entry_ID 25553 _Chem_shift_reference.ID 1 _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 water protons . . . . ppm 4.7 na indirect 0.251449530 . . . . . 25553 1 H 1 water protons . . . . ppm 4.7 internal direct 1 . . . . . 25553 1 N 15 water protons . . . . ppm 4.7 na indirect 0.101329118 . . . . . 25553 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chem_shift_list_1 _Assigned_chem_shift_list.Entry_ID 25553 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chemical_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '15N IPAP-HSQC' 1 $sample_1 isotropic 25553 1 2 '15N IPAP-HSQC' 2 $sample_2 anisotropic 25553 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 1 1 1 MET H H 1 8.647 . . 1 . . . A 1 MET H . 25553 1 2 . 1 1 1 1 MET N N 15 125.399 . . 1 . . . A 1 MET N . 25553 1 3 . 1 1 2 2 SER H H 1 8.456 . . 1 . . . A 2 SER H . 25553 1 4 . 1 1 2 2 SER CA C 13 58.536 . . 1 . . . A 2 SER CA . 25553 1 5 . 1 1 2 2 SER N N 15 116.497 . . 1 . . . A 2 SER N . 25553 1 6 . 1 1 3 3 ASP H H 1 8.291 . . 1 . . . A 3 ASP H . 25553 1 7 . 1 1 3 3 ASP N N 15 122.166 . . 1 . . . A 3 ASP N . 25553 1 8 . 1 1 4 4 GLN H H 1 8.250 . . 1 . . . A 4 GLN H . 25553 1 9 . 1 1 4 4 GLN N N 15 119.634 . . 1 . . . A 4 GLN N . 25553 1 10 . 1 1 5 5 GLU H H 1 8.278 . . 1 . . . A 5 GLU H . 25553 1 11 . 1 1 5 5 GLU N N 15 121.555 . . 1 . . . A 5 GLU N . 25553 1 12 . 1 1 6 6 ALA H H 1 8.300 . . 1 . . . A 6 ALA H . 25553 1 13 . 1 1 6 6 ALA N N 15 125.392 . . 1 . . . A 6 ALA N . 25553 1 14 . 1 1 7 7 LYS H H 1 8.345 . . 1 . . . A 7 LYS H . 25553 1 15 . 1 1 7 7 LYS CA C 13 61.997 . . 1 . . . A 7 LYS CA . 25553 1 16 . 1 1 7 7 LYS CB C 13 69.572 . . 1 . . . A 7 LYS CB . 25553 1 17 . 1 1 7 7 LYS N N 15 122.249 . . 1 . . . A 7 LYS N . 25553 1 18 . 1 1 9 9 SER H H 1 8.648 . . 1 . . . A 9 SER H . 25553 1 19 . 1 1 9 9 SER N N 15 116.955 . . 1 . . . A 9 SER N . 25553 1 20 . 1 1 10 10 THR H H 1 8.306 . . 1 . . . A 10 THR H . 25553 1 21 . 1 1 10 10 THR N N 15 115.893 . . 1 . . . A 10 THR N . 25553 1 22 . 1 1 11 11 GLU H H 1 7.801 . . 1 . . . A 11 GLU H . 25553 1 23 . 1 1 11 11 GLU CA C 13 64.005 . . 1 . . . A 11 GLU CA . 25553 1 24 . 1 1 11 11 GLU CB C 13 33.299 . . 1 . . . A 11 GLU CB . 25553 1 25 . 1 1 11 11 GLU N N 15 125.995 . . 1 . . . A 11 GLU N . 25553 1 26 . 1 1 12 12 ASP H H 1 8.413 . . 1 . . . A 12 ASP H . 25553 1 27 . 1 1 12 12 ASP CA C 13 54.261 . . 1 . . . A 12 ASP CA . 25553 1 28 . 1 1 12 12 ASP CB C 13 41.184 . . 1 . . . A 12 ASP CB . 25553 1 29 . 1 1 12 12 ASP N N 15 122.112 . . 1 . . . A 12 ASP N . 25553 1 30 . 1 1 13 13 LEU H H 1 8.448 . . 1 . . . A 13 LEU H . 25553 1 31 . 1 1 13 13 LEU CA C 13 55.626 . . 1 . . . A 13 LEU CA . 25553 1 32 . 1 1 13 13 LEU CB C 13 41.909 . . 1 . . . A 13 LEU CB . 25553 1 33 . 1 1 13 13 LEU N N 15 123.821 . . 1 . . . A 13 LEU N . 25553 1 34 . 1 1 14 14 GLY H H 1 8.486 . . 1 . . . A 14 GLY H . 25553 1 35 . 1 1 14 14 GLY N N 15 109.007 . . 1 . . . A 14 GLY N . 25553 1 36 . 1 1 15 15 ASP H H 1 8.241 . . 1 . . . A 15 ASP H . 25553 1 37 . 1 1 15 15 ASP N N 15 120.475 . . 1 . . . A 15 ASP N . 25553 1 38 . 1 1 16 16 LYS H H 1 8.341 . . 1 . . . A 16 LYS H . 25553 1 39 . 1 1 16 16 LYS N N 15 121.239 . . 1 . . . A 16 LYS N . 25553 1 40 . 1 1 17 17 LYS H H 1 8.364 . . 1 . . . A 17 LYS H . 25553 1 41 . 1 1 17 17 LYS N N 15 121.961 . . 1 . . . A 17 LYS N . 25553 1 42 . 1 1 18 18 GLU H H 1 8.552 . . 1 . . . A 18 GLU H . 25553 1 43 . 1 1 18 18 GLU N N 15 121.945 . . 1 . . . A 18 GLU N . 25553 1 44 . 1 1 19 19 GLY H H 1 8.496 . . 1 . . . A 19 GLY H . 25553 1 45 . 1 1 19 19 GLY N N 15 109.685 . . 1 . . . A 19 GLY N . 25553 1 46 . 1 1 20 20 GLU H H 1 8.243 . . 1 . . . A 20 GLU H . 25553 1 47 . 1 1 20 20 GLU N N 15 119.405 . . 1 . . . A 20 GLU N . 25553 1 48 . 1 1 21 21 TYR H H 1 8.445 . . 1 . . . A 21 TYR H . 25553 1 49 . 1 1 21 21 TYR N N 15 119.454 . . 1 . . . A 21 TYR N . 25553 1 50 . 1 1 22 22 ILE H H 1 9.150 . . 1 . . . A 22 ILE H . 25553 1 51 . 1 1 22 22 ILE N N 15 116.473 . . 1 . . . A 22 ILE N . 25553 1 52 . 1 1 23 23 LYS H H 1 8.681 . . 1 . . . A 23 LYS H . 25553 1 53 . 1 1 23 23 LYS N N 15 124.373 . . 1 . . . A 23 LYS N . 25553 1 54 . 1 1 24 24 LEU H H 1 8.972 . . 1 . . . A 24 LEU H . 25553 1 55 . 1 1 24 24 LEU N N 15 124.469 . . 1 . . . A 24 LEU N . 25553 1 56 . 1 1 25 25 LYS H H 1 8.693 . . 1 . . . A 25 LYS H . 25553 1 57 . 1 1 25 25 LYS N N 15 120.713 . . 1 . . . A 25 LYS N . 25553 1 58 . 1 1 26 26 VAL H H 1 9.109 . . 1 . . . A 26 VAL H . 25553 1 59 . 1 1 26 26 VAL N N 15 124.266 . . 1 . . . A 26 VAL N . 25553 1 60 . 1 1 27 27 ILE H H 1 9.182 . . 1 . . . A 27 ILE H . 25553 1 61 . 1 1 27 27 ILE N N 15 127.219 . . 1 . . . A 27 ILE N . 25553 1 62 . 1 1 28 28 GLY H H 1 8.474 . . 1 . . . A 28 GLY H . 25553 1 63 . 1 1 28 28 GLY N N 15 113.517 . . 1 . . . A 28 GLY N . 25553 1 64 . 1 1 29 29 GLN H H 1 8.777 . . 1 . . . A 29 GLN H . 25553 1 65 . 1 1 29 29 GLN N N 15 120.402 . . 1 . . . A 29 GLN N . 25553 1 66 . 1 1 30 30 ASP H H 1 8.395 . . 1 . . . A 30 ASP H . 25553 1 67 . 1 1 30 30 ASP N N 15 118.456 . . 1 . . . A 30 ASP N . 25553 1 68 . 1 1 31 31 SER H H 1 8.037 . . 1 . . . A 31 SER H . 25553 1 69 . 1 1 31 31 SER N N 15 111.091 . . 1 . . . A 31 SER N . 25553 1 70 . 1 1 32 32 SER H H 1 8.129 . . 1 . . . A 32 SER H . 25553 1 71 . 1 1 32 32 SER N N 15 117.531 . . 1 . . . A 32 SER N . 25553 1 72 . 1 1 33 33 GLU H H 1 8.646 . . 1 . . . A 33 GLU H . 25553 1 73 . 1 1 33 33 GLU N N 15 121.992 . . 1 . . . A 33 GLU N . 25553 1 74 . 1 1 34 34 ILE H H 1 8.752 . . 1 . . . A 34 ILE H . 25553 1 75 . 1 1 34 34 ILE N N 15 124.429 . . 1 . . . A 34 ILE N . 25553 1 76 . 1 1 35 35 HIS H H 1 8.724 . . 1 . . . A 35 HIS H . 25553 1 77 . 1 1 35 35 HIS N N 15 126.008 . . 1 . . . A 35 HIS N . 25553 1 78 . 1 1 36 36 PHE H H 1 9.152 . . 1 . . . A 36 PHE H . 25553 1 79 . 1 1 36 36 PHE N N 15 120.095 . . 1 . . . A 36 PHE N . 25553 1 80 . 1 1 37 37 LYS H H 1 8.828 . . 1 . . . A 37 LYS H . 25553 1 81 . 1 1 37 37 LYS N N 15 123.638 . . 1 . . . A 37 LYS N . 25553 1 82 . 1 1 38 38 VAL H H 1 9.199 . . 1 . . . A 38 VAL H . 25553 1 83 . 1 1 38 38 VAL N N 15 119.691 . . 1 . . . A 38 VAL N . 25553 1 84 . 1 1 40 40 MET H H 1 8.636 . . 1 . . . A 40 MET H . 25553 1 85 . 1 1 40 40 MET N N 15 120.339 . . 1 . . . A 40 MET N . 25553 1 86 . 1 1 41 41 THR H H 1 7.004 . . 1 . . . A 41 THR H . 25553 1 87 . 1 1 41 41 THR N N 15 130.421 . . 1 . . . A 41 THR N . 25553 1 88 . 1 1 42 42 THR H H 1 7.212 . . 1 . . . A 42 THR H . 25553 1 89 . 1 1 42 42 THR N N 15 120.469 . . 1 . . . A 42 THR N . 25553 1 90 . 1 1 43 43 HIS H H 1 8.236 . . 1 . . . A 43 HIS H . 25553 1 91 . 1 1 43 43 HIS N N 15 116.909 . . 1 . . . A 43 HIS N . 25553 1 92 . 1 1 44 44 LEU H H 1 9.373 . . 1 . . . A 44 LEU H . 25553 1 93 . 1 1 44 44 LEU N N 15 124.587 . . 1 . . . A 44 LEU N . 25553 1 94 . 1 1 45 45 LYS H H 1 8.581 . . 1 . . . A 45 LYS H . 25553 1 95 . 1 1 45 45 LYS N N 15 121.350 . . 1 . . . A 45 LYS N . 25553 1 96 . 1 1 46 46 LYS H H 1 7.447 . . 1 . . . A 46 LYS H . 25553 1 97 . 1 1 46 46 LYS N N 15 114.002 . . 1 . . . A 46 LYS N . 25553 1 98 . 1 1 47 47 LEU H H 1 6.757 . . 1 . . . A 47 LEU H . 25553 1 99 . 1 1 47 47 LEU N N 15 121.848 . . 1 . . . A 47 LEU N . 25553 1 100 . 1 1 48 48 LYS H H 1 7.641 . . 1 . . . A 48 LYS H . 25553 1 101 . 1 1 48 48 LYS N N 15 118.450 . . 1 . . . A 48 LYS N . 25553 1 102 . 1 1 49 49 GLU H H 1 8.601 . . 1 . . . A 49 GLU H . 25553 1 103 . 1 1 49 49 GLU N N 15 120.214 . . 1 . . . A 49 GLU N . 25553 1 104 . 1 1 50 50 SER H H 1 8.066 . . 1 . . . A 50 SER H . 25553 1 105 . 1 1 50 50 SER N N 15 116.063 . . 1 . . . A 50 SER N . 25553 1 106 . 1 1 51 51 TYR H H 1 8.990 . . 1 . . . A 51 TYR H . 25553 1 107 . 1 1 51 51 TYR N N 15 121.658 . . 1 . . . A 51 TYR N . 25553 1 108 . 1 1 52 52 CYS H H 1 8.454 . . 1 . . . A 52 CYS H . 25553 1 109 . 1 1 52 52 CYS N N 15 117.818 . . 1 . . . A 52 CYS N . 25553 1 110 . 1 1 53 53 GLN H H 1 8.302 . . 1 . . . A 53 GLN H . 25553 1 111 . 1 1 53 53 GLN N N 15 118.730 . . 1 . . . A 53 GLN N . 25553 1 112 . 1 1 54 54 ARG H H 1 7.984 . . 1 . . . A 54 ARG H . 25553 1 113 . 1 1 54 54 ARG N N 15 120.164 . . 1 . . . A 54 ARG N . 25553 1 114 . 1 1 55 55 GLN H H 1 7.796 . . 1 . . . A 55 GLN H . 25553 1 115 . 1 1 55 55 GLN N N 15 114.277 . . 1 . . . A 55 GLN N . 25553 1 116 . 1 1 56 56 GLY H H 1 7.927 . . 1 . . . A 56 GLY H . 25553 1 117 . 1 1 56 56 GLY N N 15 109.154 . . 1 . . . A 56 GLY N . 25553 1 118 . 1 1 57 57 VAL H H 1 7.586 . . 1 . . . A 57 VAL H . 25553 1 119 . 1 1 57 57 VAL N N 15 112.596 . . 1 . . . A 57 VAL N . 25553 1 120 . 1 1 59 59 MET H H 1 8.658 . . 1 . . . A 59 MET H . 25553 1 121 . 1 1 59 59 MET N N 15 125.439 . . 1 . . . A 59 MET N . 25553 1 122 . 1 1 60 60 ASN H H 1 8.644 . . 1 . . . A 60 ASN H . 25553 1 123 . 1 1 60 60 ASN N N 15 113.488 . . 1 . . . A 60 ASN N . 25553 1 124 . 1 1 61 61 SER H H 1 7.972 . . 1 . . . A 61 SER H . 25553 1 125 . 1 1 61 61 SER N N 15 114.354 . . 1 . . . A 61 SER N . 25553 1 126 . 1 1 62 62 LEU H H 1 7.500 . . 1 . . . A 62 LEU H . 25553 1 127 . 1 1 62 62 LEU N N 15 121.938 . . 1 . . . A 62 LEU N . 25553 1 128 . 1 1 63 63 ARG H H 1 8.989 . . 1 . . . A 63 ARG H . 25553 1 129 . 1 1 63 63 ARG N N 15 121.523 . . 1 . . . A 63 ARG N . 25553 1 130 . 1 1 64 64 PHE H H 1 8.922 . . 1 . . . A 64 PHE H . 25553 1 131 . 1 1 64 64 PHE N N 15 123.031 . . 1 . . . A 64 PHE N . 25553 1 132 . 1 1 65 65 LEU H H 1 9.765 . . 1 . . . A 65 LEU H . 25553 1 133 . 1 1 65 65 LEU CA C 13 53.268 . . 1 . . . A 65 LEU CA . 25553 1 134 . 1 1 65 65 LEU N N 15 124.233 . . 1 . . . A 65 LEU N . 25553 1 135 . 1 1 66 66 PHE H H 1 8.992 . . 1 . . . A 66 PHE H . 25553 1 136 . 1 1 66 66 PHE N N 15 119.418 . . 1 . . . A 66 PHE N . 25553 1 137 . 1 1 67 67 GLU H H 1 9.421 . . 1 . . . A 67 GLU H . 25553 1 138 . 1 1 67 67 GLU N N 15 129.229 . . 1 . . . A 67 GLU N . 25553 1 139 . 1 1 68 68 GLY H H 1 8.901 . . 1 . . . A 68 GLY H . 25553 1 140 . 1 1 68 68 GLY N N 15 133.446 . . 1 . . . A 68 GLY N . 25553 1 141 . 1 1 69 69 GLN H H 1 8.026 . . 1 . . . A 69 GLN H . 25553 1 142 . 1 1 69 69 GLN N N 15 120.668 . . 1 . . . A 69 GLN N . 25553 1 143 . 1 1 70 70 ARG H H 1 8.842 . . 1 . . . A 70 ARG H . 25553 1 144 . 1 1 70 70 ARG N N 15 124.718 . . 1 . . . A 70 ARG N . 25553 1 145 . 1 1 71 71 ILE H H 1 8.943 . . 1 . . . A 71 ILE H . 25553 1 146 . 1 1 71 71 ILE N N 15 126.632 . . 1 . . . A 71 ILE N . 25553 1 147 . 1 1 72 72 ALA H H 1 11.082 . . 1 . . . A 72 ALA H . 25553 1 148 . 1 1 72 72 ALA N N 15 109.283 . . 1 . . . A 72 ALA N . 25553 1 149 . 1 1 73 73 ASP H H 1 8.813 . . 1 . . . A 73 ASP H . 25553 1 150 . 1 1 73 73 ASP N N 15 120.436 . . 1 . . . A 73 ASP N . 25553 1 151 . 1 1 74 74 ASN H H 1 7.754 . . 1 . . . A 74 ASN H . 25553 1 152 . 1 1 74 74 ASN N N 15 109.502 . . 1 . . . A 74 ASN N . 25553 1 153 . 1 1 75 75 HIS H H 1 7.152 . . 1 . . . A 75 HIS H . 25553 1 154 . 1 1 75 75 HIS CB C 13 33.557 . . 1 . . . A 75 HIS CB . 25553 1 155 . 1 1 75 75 HIS N N 15 119.774 . . 1 . . . A 75 HIS N . 25553 1 156 . 1 1 76 76 THR H H 1 7.353 . . 1 . . . A 76 THR H . 25553 1 157 . 1 1 76 76 THR CA C 13 57.529 . . 1 . . . A 76 THR CA . 25553 1 158 . 1 1 76 76 THR N N 15 108.335 . . 1 . . . A 76 THR N . 25553 1 159 . 1 1 78 78 LYS H H 1 8.118 . . 1 . . . A 78 LYS H . 25553 1 160 . 1 1 78 78 LYS N N 15 115.350 . . 1 . . . A 78 LYS N . 25553 1 161 . 1 1 79 79 GLU H H 1 7.980 . . 1 . . . A 79 GLU H . 25553 1 162 . 1 1 79 79 GLU CB C 13 36.135 . . 1 . . . A 79 GLU CB . 25553 1 163 . 1 1 79 79 GLU N N 15 120.853 . . 1 . . . A 79 GLU N . 25553 1 164 . 1 1 80 80 LEU H H 1 7.488 . . 1 . . . A 80 LEU H . 25553 1 165 . 1 1 80 80 LEU N N 15 115.048 . . 1 . . . A 80 LEU N . 25553 1 166 . 1 1 81 81 GLY H H 1 7.680 . . 1 . . . A 81 GLY H . 25553 1 167 . 1 1 81 81 GLY N N 15 108.185 . . 1 . . . A 81 GLY N . 25553 1 168 . 1 1 82 82 MET H H 1 7.679 . . 1 . . . A 82 MET H . 25553 1 169 . 1 1 82 82 MET N N 15 117.789 . . 1 . . . A 82 MET N . 25553 1 170 . 1 1 83 83 GLU H H 1 9.294 . . 1 . . . A 83 GLU H . 25553 1 171 . 1 1 83 83 GLU N N 15 120.994 . . 1 . . . A 83 GLU N . 25553 1 172 . 1 1 84 84 GLU H H 1 8.338 . . 1 . . . A 84 GLU H . 25553 1 173 . 1 1 84 84 GLU N N 15 119.709 . . 1 . . . A 84 GLU N . 25553 1 174 . 1 1 85 85 GLU H H 1 9.389 . . 1 . . . A 85 GLU H . 25553 1 175 . 1 1 85 85 GLU CA C 13 58.580 . . 1 . . . A 85 GLU CA . 25553 1 176 . 1 1 85 85 GLU N N 15 118.776 . . 1 . . . A 85 GLU N . 25553 1 177 . 1 1 86 86 ASP H H 1 7.977 . . 1 . . . A 86 ASP H . 25553 1 178 . 1 1 86 86 ASP N N 15 120.870 . . 1 . . . A 86 ASP N . 25553 1 179 . 1 1 87 87 VAL H H 1 8.397 . . 1 . . . A 87 VAL H . 25553 1 180 . 1 1 87 87 VAL N N 15 114.496 . . 1 . . . A 87 VAL N . 25553 1 181 . 1 1 88 88 ILE H H 1 9.225 . . 1 . . . A 88 ILE H . 25553 1 182 . 1 1 88 88 ILE N N 15 127.112 . . 1 . . . A 88 ILE N . 25553 1 183 . 1 1 89 89 GLU H H 1 8.864 . . 1 . . . A 89 GLU H . 25553 1 184 . 1 1 89 89 GLU N N 15 125.965 . . 1 . . . A 89 GLU N . 25553 1 185 . 1 1 90 90 VAL H H 1 7.891 . . 1 . . . A 90 VAL H . 25553 1 186 . 1 1 90 90 VAL N N 15 120.387 . . 1 . . . A 90 VAL N . 25553 1 187 . 1 1 92 92 GLN H H 1 8.765 . . 1 . . . A 92 GLN H . 25553 1 188 . 1 1 92 92 GLN N N 15 120.844 . . 1 . . . A 92 GLN N . 25553 1 189 . 1 1 93 93 GLU H H 1 8.647 . . 1 . . . A 93 GLU H . 25553 1 190 . 1 1 93 93 GLU N N 15 125.444 . . 1 . . . A 93 GLU N . 25553 1 191 . 1 1 94 94 GLN H H 1 8.817 . . 1 . . . A 94 GLN H . 25553 1 192 . 1 1 94 94 GLN N N 15 122.909 . . 1 . . . A 94 GLN N . 25553 1 193 . 1 1 95 95 THR H H 1 8.445 . . 1 . . . A 95 THR H . 25553 1 194 . 1 1 95 95 THR N N 15 115.903 . . 1 . . . A 95 THR N . 25553 1 195 . 1 1 96 96 GLY H H 1 8.561 . . 1 . . . A 96 GLY H . 25553 1 196 . 1 1 96 96 GLY N N 15 111.388 . . 1 . . . A 96 GLY N . 25553 1 197 . 1 1 97 97 HIS H H 1 8.355 . . 1 . . . A 97 HIS HN . 25553 1 198 . 1 1 97 97 HIS N N 15 108.599 . . 1 . . . A 97 HIS N . 25553 1 stop_ save_