# 1 "/u/exp/stan/nmr/lists/pp/ko_cryo_HBHACONH3d.6" ; ko_hbhaconh3d ;3D HBHACONH # 1 "/u/exp/stan/nmr/lists/pp/Avance.incl" 1 ;Avance.incl ; ;avance-version (03/02/17) ;$Id: Avance1.incl,v 1.7.2.3 2003/02/25 14:48:47 ber Exp $ # 6 "/u/exp/stan/nmr/lists/pp/ko_cryo_HBHACONH3d.6" 2 # 1 "/u/exp/stan/nmr/lists/pp/Grad.incl" 1 ;Grad.incl - include file for Gradient Spectroscopy ; ;avance-version (02/05/31) define list EA= ;$Id: Grad1.incl,v 1.7 2002/06/12 09:04:22 ber Exp $ # 7 "/u/exp/stan/nmr/lists/pp/ko_cryo_HBHACONH3d.6" 2 # 1 "/u/exp/stan/nmr/lists/pp/Delay.incl" 1 ;Delay.incl - include file for commonly used delays ; ;version 00/02/07 ;general delays define delay DELTA define delay DELTA1 define delay DELTA2 define delay DELTA3 define delay DELTA4 define delay DELTA5 define delay DELTA6 define delay DELTA7 define delay DELTA8 define delay TAU define delay TAU1 define delay TAU2 define delay TAU3 define delay TAU4 define delay TAU5 ;delays for centering pulses define delay CEN_HN1 define delay CEN_HN2 define delay CEN_HN3 define delay CEN_HC1 define delay CEN_HC2 define delay CEN_HC3 define delay CEN_HC4 define delay CEN_HP1 define delay CEN_HP2 define delay CEN_CN1 define delay CEN_CN2 define delay CEN_CN3 define delay CEN_CN4 define delay CEN_CP1 define delay CEN_CP2 ;loop counters define loopcounter COUNTER define loopcounter SCALEF define loopcounter FACTOR1 define loopcounter FACTOR2 define loopcounter FACTOR3 ;$Id: Delay.incl,v 1.11 2002/06/12 09:04:22 ber Exp $ # 8 "/u/exp/stan/nmr/lists/pp/ko_cryo_HBHACONH3d.6" 2 "p22=p21*2.0" "p2=p1*2.0" "d3=2.1m" ;tau b "d4=1.5m-p19" ;tau a "d22=3.1m" ;T C "d23=3.6m" ;tau c ; 15N-1H transfer delay "d2=2.25m" "d5=5.4m" "d24=0.2m" "d25=d2-p17-d24-p5-140u" "d7=p7" "d17=4.4m" ;tau d "d27=11.4m" ;tau e "d28=d27-p15-4u" "d13=p26+8u" "d19=d22" "DELTA1=d22-d3-p1*2-p7-33.5u" "DELTA2=d23-p24-4u" "DELTA3=d27-d17-p15" ; Initial Delay of Evolution Time "d0=4u" "d10=6.2m-d5-p15/2.0-d7-9.5u" "d30=6.2m-p15/2.0-4u" "in30=in10" ; Centering Delay "CEN_HN2=(p22-p2)/2" "CEN_CN2=(p24-p22)/2" "l3=td1/2" "l13=td2/2" "spoff0=0" "spoff2=0" "spoff3=0" "spoff4=bf2*((cnst21-cnst23)/1000000)" "spoff5=bf2*((cnst22-cnst21)/1000000)" "spoff7=0" "spoff8=0" 1 ze 2 80m do:f3 3 30m 4 30m 5 30m 6 d1 fq=cnst23(bf ppm):f2 20u pl1:f1 20u pl3:f3 20u pl2:f2 50u setnmr2|0 setnmr0|34|32|33 (p1 ph7):f1 5u ; p19:gp1 ; 95u ; d4 d0 (p26:sp8 ph0):f2 ; CAB 180deg REBURP d0 (p2 ph0):f1 5u ; p19:gp1 ; 95u ; d4 d13 (p1 ph9):f1 4u ;------------- 13CB->13CA transfer. (p13:sp0 ph11):f2 ; CAB 90deg rect50 d3 (p1 ph9):f1 4u (p1 ph8):f1 20u pl19:f1 (p7 ph4):f1 5.5u 4u cpds1:f1 DELTA1 (p23:sp7 ph14):f2 ; CAB 180deg. REBURP d19 (p13:sp0 ph0):f2 ; CAB 90deg. rect50 ;------------- 13CA->13CO transfer. 4u (p24:sp4 ph0):f2 ; CO 180deg G3 DELTA2 (p23:sp7 ph16):f2 ; CAB 180deg. REBURP 4u (p24:sp4 ph0):f2 ; CO 180deg. G3 DELTA2 (p13:sp0 ph0):f2 ; CAB 90deg. rect50 ;------------- 4u do:f1 5.5u (p7 ph4):f1 ; ; 5u ; p18:gp4 ; 200u ; ; ; 30u fq=cnst21(bf ppm):f2 (p7 ph3):f1 5.5u 4u cpds1:f1 ;------------- 13CO->15N transfer. (p14:sp3 ph19):f2 ; CO 90deg. rect50 d17 (p15:sp5 ph0):f2 ; CA 180deg. rSNOB DELTA3 (p24:sp2 ph0):f2 (CEN_CN2 p22 ph0):f3 ; CO 180deg. N 180deg. rSNOB d28 (p15:sp5 ph0):f2 ; CA 180deg. rSNOB 4u (p14:sp3 ph0):f2 ; CO 90deg. rect50 4u ;------------- 15N evolution. (p21 ph13):f3 ; N 90deg. 4u ;pl2:f2 d30 (p15:sp5 ph0):f2 4u ;pl2:f2 d30 (p24:sp2 ph0):f2 (CEN_CN2 p22 ph17):f3 ; CO 180deg. N 180deg. rSNOB d10 4u ;pl2:f2 5.5u d7 d5 (p15:sp5 ph0):f2 d10 4u do:f1 5.5u (p7 ph4):f1 d5 (p21 ph0):f3 ;------------- 15N->1H transfer. 5u ; p16:gp2 ; Homospoil 195u ; 20u pl5:f1 (p5 ph27:r):f1 20u pl1:f1 ;------------- (p1 ph0):f1 d24 5u ; p17:gp3 ; 95u ; d25 20u pl5:f1 (p5 ph28:r):f1 20u pl1:f1 (CEN_HN2 p2 ph0):f1 (p22 ph0):f3 20u pl5:f1 (p5 ph29:r):f1 20u pl1:f1 d25 5u ; p17:gp3 ; 75u ; 20u setnmr2^0 setnmr0^34^32^33 d24 pl13:f3 go=2 ph31 cpd3:f3 60m do:f3 wr #0 if #0 zd 10m ip7 10m ip8 lo to 3 times 2 15m id0 15m ip31*2 lo to 4 times l3 15m rd0 15m dp13 lo to 5 times 2 10m id10 10m dd30 10m ip31*2 lo to 6 times l13 30m setnmr2^0 setnmr0^34^32^33 exit ; General pulse phase ph0=0 ; 1H pulse phase ph1=0 ph7=0 ph8=2 ph4=1 ph9=1 1 3 3 ph3=3 ph27=2 ph28=2 ph29=2 ; 13C pulse phase ph11=0 ph14=0 ph16=0 ph19=0 0 0 0 2 2 2 2 ; 15N pulse phase ph13=0 2 ph17=0 ; Reciever phase ph31=0 2 2 0 2 0 0 2 ;for 1H ;p1:1H hard pulse ~10us ;pl1:1H hard pulse ;p5:1H wfb pulse [1ms] (phcor27,28,29) ;pl5:water flip back ;pcpd1:1H CPD ~56us @pl19 ;p7:1H CPD ~56us @pl19 ;pl19:1H CPD pulse ; ; ;for 15N ;p21:15N hard pulse ~50us ;pl3:15N hard pulse ;pcpd3:15N CPD pulse ~250us ;pl13:15N CPD pulse ; ;for 13C ;sp0:CAB 90deg rect (p13) ;sp7:CAB 180deg G3.256 (p23) ;sp5:CA off 180deg rSNOB (p15) ;sp3:CO 90deg rect50 (p14) ;sp2:CO 180deg Seduce (p24) ;sp8:CAB 180deg rect (p26) ;sp4:CO off 180deg Seduce (p24) ; ;p13:CAB 90deg rect @~48u ;p14:CO 90deg rect @~53u ;p23:CAB 180deg G3.256 @256u ;p24:CO 180deg Seduce @192u ;p15:CA 180deg rSNOB @367u ;p26:CAB 180deg rect @~21*2u ;cnst21: CO chemical shift (offset, in ppm) ;cnst22: CA chemical shift (offset, in ppm) ;cnst23: CAB chemical shift (offset, in ppm) ;o2p: CAB chemical shift (cnst23) ;for gradient ;p19:500u gp1 : echo ;p16:1.7m gp2 : homospoil ;p17:500u gp3 : echo ;p18:1.0m gp4 : homospoil