# 1 "/Bruker/35/exp/stan/nmr/lists/pp/ko_HSQCWFBC.3" ; HSQC using the WFB method ; Junji Iwahara # 1 "/Bruker/35/exp/stan/nmr/lists/pp/Avance.incl" 1 ;Avance2.incl ; for 1 ; ;avance-version (03/02/17) ;$Id: Avance2.incl,v 1.7.2.3 2003/02/25 14:48:48 ber Exp $ # 5 "/Bruker/35/exp/stan/nmr/lists/pp/ko_HSQCWFBC.3" 2 # 1 "/Bruker/35/exp/stan/nmr/lists/pp/Grad.incl" 1 ;Grad2.incl - include file for Gradient Spectroscopy ; for 1 ; ;avance-version (02/05/31) define list EA= ;$Id: Grad2.incl,v 1.7 2002/06/12 09:04:22 ber Exp $ # 6 "/Bruker/35/exp/stan/nmr/lists/pp/ko_HSQCWFBC.3" 2 ; PFG length ; "p15=1.8m" ; "p16=1.4m" ; "p17=500u" ; transfer delay "d2=2.25m" ;defined parameters. "d5=p21-p1" ;"d6=p24/2.0-p1" "d24=d2-p17-p5-125u" "d15=p24/2.0-p1" "d16=p24/2.0-p21" ;initial delay ;"d0=in0*1.0-p21*2.0/3.14-p24/2.0" "d0=in0*0.5-p21*2.0/3.14" ;"d0=3u" "l1=td1/2" ;pulse sequence. 30m 1 ze 2 60m do:f3 3 30m 4 20u d1 pl1:f1 (p1 ph0):f1 d2 pl3:f3 (d5 p1*2.0 ph1):f1 (p21*2.0 ph10):f3 d2 setnmr3|0 setnmr0|34|32|33 (p1 ph1):f1 5u 20u pl5:f1 (p5 ph5:r):f1 5u ; p15:gp1 ; 800u ; 20u pl1:f1 20u pl2:f2 (d16 p21 ph11 d0 d0 p21 ph13 d16):f3 (d0 p24:sp1 ph10):f2 (d0 d15 p1*2.0 ph0):f1 ; (p21 ph11):f3 ; d0 ; (d6 p1*2.0 ph0):f1 (p24:sp1 ph10):f2 ; d0 ; (p21 ph12):f3 5u ; p16:gp2 ; 800u ; 20u pl5:f1 (p5 ph7:r):f1 5u 20u pl1:f1 (p1 ph0):f1 5u 5u ; p17:gp3 ; 95u ; d24 pl5:f1 (p5 ph8:r):f1 20u pl1:f1 (d5 p1*2.0 ph3):f1 (p21*2.0 ph12):f3 20u pl5:f1 (p5 ph8:r):f1 5u ; p17:gp3 ; 95u ; d24 pl13:f3 5u setnmr3^0 setnmr0^34^32^33 go=2 ph31 cpd3:f3 60m do:f3 wr #0 if #0 ip11 zd lo to 3 times 2 15m id0 15m ip31*2 lo to 4 times l1 30m setnmr3^0 setnmr0^34^32^33 exit ;-- 1H pulse -- ph0=0 ph1=1 1 3 3 ph3=2 ;-- Water-selective pulse -- ph5=(360) 0 ; 0 deg ph7=(360) 180 ;180 deg ph8=(360) 0 ; 0 deg ;-- 15N pulses -- ph10=0 ph11=0 2 ph12=0 ph13=0 0 0 0 2 2 2 2 ;-- phases for receiver -- ;ph31=0 2 2 0 ph31=0 2 2 0 2 0 0 2 ;pl1: 1H hard pulse -1~0dB ;p1: 1H hard pulse 9~10us ;pl5: wfb pulse 39~40dB (phcor5,7,8) ;p5: wfb pulse ~1ms ;pl2: 13C shaped pulse ;p24: 13C shaped pulse ;sp1: 13C shaped pulse ;pl3: 15N pulse ;p21: 15N pulse 40~50us ;pl13: 15N decouple pulse ;pcpd3: 15N decouple pulse ~250us