# 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d" ;hncacogp3d ;avance-version (07/04/04) ;HN(CA)CO ;3D sequence with ; inverse correlation for triple resonance using multiple ; inept transfer steps ; ; F1(H) -> F3(N) -> F2(Ca) -> F2(C=O,t1) ; -> F2(Ca) -> F3(N,t2) -> F1(H,t3) ; ;on/off resonance Ca and C=O pulses using shaped pulse ;phase sensitive (t1) ;phase sensitive using Echo/Antiecho gradient selection (t2) ;using constant time in t2 ;(use parameterset HNCACOGP3D) ; ;R.T. Clubb, V. Thanabal & G. Wagner, J. Magn. Reson. 97, 213-217 (1992) ;(L.E. Kay, G.Y. Xu & T. Yamazaki, J. Magn. Reson. A109, 129-133 (1994)) ; ;$CLASS=HighRes ;$DIM=3D ;$TYPE= ;$SUBTYPE= ;$COMMENT= prosol relations= # 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Avance.incl" 1 ;Avance2.incl ; for 1 ; ;avance-version (07/08/21) ; ;$CLASS=HighRes Incl ;$COMMENT= ;$Id: Avance2.incl,v 1.17.2.1 2007/09/14 16:17:35 ber Exp $ # 30 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d" 2 # 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Grad.incl" 1 ;Grad2.incl - include file for Gradient Spectroscopy ; for 1 ; ;avance-version (07/01/17) ; ;$CLASS=HighRes Incl ;$COMMENT= define list EA= ;$Id: Grad2.incl,v 1.12 2007/01/22 14:22:35 ber Exp $ # 31 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d" 2 # 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Delay.incl" 1 ;Delay.incl - include file for commonly used delays ; ;version 00/02/07 ; ;$CLASS=HighRes Incl ;$COMMENT= ;general delays define delay DELTA define delay DELTA1 define delay DELTA2 define delay DELTA3 define delay DELTA4 define delay DELTA5 define delay DELTA6 define delay DELTA7 define delay DELTA8 define delay TAU define delay TAU1 define delay TAU2 define delay TAU3 define delay TAU4 define delay TAU5 ;delays for centering pulses define delay CEN_HN1 define delay CEN_HN2 define delay CEN_HN3 define delay CEN_HC1 define delay CEN_HC2 define delay CEN_HC3 define delay CEN_HC4 define delay CEN_HP1 define delay CEN_HP2 define delay CEN_CN1 define delay CEN_CN2 define delay CEN_CN3 define delay CEN_CN4 define delay CEN_CP1 define delay CEN_CP2 ;loop counters define loopcounter COUNTER define loopcounter SCALEF define loopcounter FACTOR1 define loopcounter FACTOR2 define loopcounter FACTOR3 ;$Id: Delay.incl,v 1.12 2005/11/10 12:16:58 ber Exp $ # 32 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d" 2 "p2=p1*2" "p22=p21*2" "d11=30m" "d13=4u" "d21=5.5m" "d22=4m" "d23=12m" "d26=2.3m" "d0=3u" "d10=d23/2-p14/2" "d29=d23/2-p14/2-p26-d21-4u" "d30=d23/2-p14/2" "in0=inf1/2" "in10=inf2/4" "in29=in10" "in30=in10" "DELTA1=p16+d16+d13+4u" "DELTA2=d23-d21-p26" "DELTA3=d22-p14-4u" "DELTA4=d0*2+larger(p14,p22)-p14" "DELTA5=d21-p16-d16-4u" "spoff2=0" "spoff3=0" "spoff5=bf2*((cnst21-cnst22)/1000000)" "spoff7=bf2*((cnst22-cnst21)/1000000)" "spoff8=0" "spoff9=0" aqseq 321 # 1 "mc_line 76 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d expanding definition part of mc command before ze" ; dimension 3 aq-mode (F2) Echo-Antiecho (F1) States-TPPI F2->F1 define delay MCWRK define delay MCREST define loopcounter ST1CNT "ST1CNT = td1 / (2)" define loopcounter ST2CNT "ST2CNT = td2 / (2)" "MCWRK = 0.090909*d11" "MCREST = d11 - d11" dccorr # 76 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d" 1 d11 ze # 1 "mc_line 76 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d expanding definition of mc command after ze" # 77 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d" d11 pl16:f3 # 1 "mc_line 78 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d expanding start label for mc command" 2 MCWRK * 2 do:f3 LBLSTS2, MCWRK * 3 LBLF2, MCWRK * 4 LBLSTS1, MCWRK LBLF1, MCWRK MCREST # 79 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d" 3 d1 pl1:f1 fq=cnst22(bf ppm):f2 p1 ph1 d26 pl3:f3 (center (p2 ph1) (p22 ph1):f3 ) d26 setnmr3|0 setnmr0|34|32|33 ctrlgrad 0 (p1 ph2):f1 4u pl0:f1 (p11:sp1 ph1:r):f1 4u p16:gp1 d16 (p21 ph1):f3 d21 pl19:f1 (p26 ph2):f1 DELTA2 cpds1:f1 ph1 (center (p14:sp3 ph1):f2 (p22 ph1):f3 ) d23 (p21 ph1):f3 (p13:sp2 ph3):f2 4u (p14:sp5 ph1):f2 DELTA3 (p24:sp9 ph1):f2 4u (p14:sp5 ph1):f2 DELTA3 (p13:sp8 ph2):f2 4u 30u fq=cnst21(bf ppm):f2 (p13:sp2 ph4):f2 d0 (center (p14:sp7 ph1):f2 (p22 ph8):f3 ) d0 4u (p14:sp3 ph1):f2 DELTA4 (p14:sp7 ph1):f2 4u (p13:sp8 ph1):f2 4u 30u fq=cnst22(bf ppm):f2 (p13:sp2 ph2):f2 DELTA3 (p14:sp5 ph1):f2 4u (p24:sp9 ph1):f2 DELTA3 (p14:sp5 ph1):f2 4u (p13:sp8 ph1):f2 (p21 ph1):f3 d30 (p14:sp5 ph1):f2 d30 (center (p14:sp3 ph1):f2 (p22 ph8):f3 ) d10 (p14:sp5 ph1):f2 d29 4u do:f1 (p26 ph7):f1 4u p16:gp2*EA d16 DELTA5 pl1:f1 (center (p1 ph1) (p21 ph5):f3 ) d26 (center (p2 ph1) (p22 ph1):f3 ) d26 (center (p1 ph2) (p21 ph6):f3 ) d26 (center (p2 ph1) (p22 ph1):f3 ) d26 (p1 ph1) DELTA1 (p2 ph1) d13 p16:gp3 d16 pl16:f3 4u setnmr3^0 setnmr0^34^32^33 ctrlgrad 7 go=2 ph31 cpd3:f3 # 1 "mc_line 168 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d expanding mc command in line" MCWRK do:f3 wr #0 if #0 zd igrad EA MCWRK ip6*2 lo to LBLSTS2 times 2 MCWRK id10 MCWRK id29 MCWRK dd30 lo to LBLF2 times ST2CNT MCWRK rd10 MCWRK rd29 MCWRK rd30 MCWRK ip4 lo to LBLSTS1 times 2 MCWRK id0 lo to LBLF1 times ST1CNT MCWRK # 171 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hncacogp3d" exit ph1=0 ph2=1 ph3=0 0 0 0 0 0 0 0 2 2 2 2 2 2 2 2 ph4=0 2 ph5=0 0 2 2 ph6=3 3 1 1 ph7=3 ph8=0 0 0 0 2 2 2 2 ph31=0 2 2 0 0 2 2 0 2 0 0 2 2 0 0 2 ;pl0 : 120dB ;pl1 : f1 channel - power level for pulse (default) ;pl3 : f3 channel - power level for pulse (default) ;pl16: f3 channel - power level for CPD/BB decoupling ;pl19: f1 channel - power level for CPD/BB decoupling ;sp1: f1 channel - shaped pulse 90 degree (H2O on resonance) ;sp2: f2 channel - shaped pulse 90 degree (on resonance) ;sp3: f2 channel - shaped pulse 180 degree (on resonance) ;sp5: f2 channel - shaped pulse 180 degree (C=O off resonance) ;sp7: f2 channel - shaped pulse 180 degree (Ca off resonance) ;sp8: f2 channel - shaped pulse 90 degree (on resonance) ; for time reversed pulse ;sp9: f2 channel - shaped pulse 180 degree (Ca on resonance) ; sp9 requires higher selectivity than sp3 ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p11: f1 channel - 90 degree shaped pulse [2 msec] ;p13: f2 channel - 90 degree shaped pulse ;p14: f2 channel - 180 degree shaped pulse ;p16: homospoil/gradient pulse [1 msec] ;p21: f3 channel - 90 degree high power pulse ;p22: f3 channel - 180 degree high power pulse ;p24: f2 channel - 180 degree shaped pulse (sp9) ;p26: f1 channel - 90 degree pulse at pl19 ;d0 : incremented delay (F1 in 3D) [3 usec] ;d1 : relaxation delay; 1-5 * T1 ;d10: incremented delay (F2 in 3D) = d23/2-p14/2 ;d11: delay for disk I/O [30 msec] ;d13: short delay [4 usec] ;d16: delay for homospoil/gradient recovery ;d21: 1/(2J(NH) [5.5 msec] ;d22: 1/(4J(CaCO) [4 msec] ;d23: 1/(4J(NCa) [12 msec] ;d26: 1/(4J'(NH) [2.3 msec] ;d29: incremented delay (F2 in 3D) = d23/2-p14/2-p26-d21-4u ;d30: decremented delay (F2 in 3D) = d23/2-p14/2 ;cnst21: CO chemical shift (offset, in ppm) ;cnst22: Calpha chemical shift (offset, in ppm) ;o2p: CO chemical shift (cnst21) ;inf1: 1/SW(CO) = 2 * DW(CO) ;inf2: 1/SW(N) = 2 * DW(N) ;in0: 1/(2 * SW(CO)) = DW(CO) ;nd0: 2 ;in10: 1/(4 * SW(N)) = (1/2) DW(N) ;nd10: 4 ;in29: = in10 ;in30: = in10 ;NS: 8 * n ;DS: >= 16 ;td1: number of experiments in F1 ;td2: number of experiments in F2 td2 max = 2 * d30 / in30 ;FnMODE: States-TPPI (or TPPI) in F1 ;FnMODE: echo-antiecho in F2 ;cpds1: decoupling according to sequence defined by cpdprg1 ;cpd3: decoupling according to sequence defined by cpdprg3 ;pcpd1: f1 channel - 90 degree pulse for decoupling sequence ;pcpd3: f3 channel - 90 degree pulse for decoupling sequence ;use gradient ratio: gp 1 : gp 2 : gp 3 ; 30 : 80 : 8.1 ;for z-only gradients: ;gpz1: 30% ;gpz2: 80% ;gpz3: 8.1% ;use gradient files: ;gpnam1: SINE.100 ;gpnam2: SINE.100 ;gpnam3: SINE.100 ;$Id: hncacogp3d,v 1.14 2007/04/11 13:34:30 ber Exp $