# 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi" ;trosyetf3gpsi ;avance-version (07/04/04) ;2D H-1/X correlation via TROSY ; using sensitivity improvement ;phase sensitive using Echo/Antiecho gradient selection ;using f3 - channel ;(use parameterset TROSYETF3GPSI) ; ;M. Czisch & R. Boelens, J. Magn. Reson. 134, 158-160 (1998) ;K. Pervushin, G. Wider & K. Wuethrich, J. Biomol. NMR 12, ; 345-348 (1998) ;A. Meissner, T. Schulte-Herbrueggen, J. Briand & O.W. Sorensen, Mol. Phys. 96, ; 1137-1142 (1998) ;J. Weigelt, J. Am. Chem. Soc. 120, 10778-10779 (1998) ;M. Rance, J.P. Loria & A.G. Palmer III, J. Magn. Reson. 136, 91-101 (1999) ;G. Zhu, X.M. Kong & K.H. Sze, J. Biomol. NMR 13, 77-81 (1999) ; ;$CLASS=HighRes ;$DIM=2D ;$TYPE= ;$SUBTYPE= ;$COMMENT= # 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Avance.incl" 1 ;Avance2.incl ; for 1 ; ;avance-version (07/08/21) ; ;$CLASS=HighRes Incl ;$COMMENT= ;$Id: Avance2.incl,v 1.17.2.1 2007/09/14 16:17:35 ber Exp $ # 25 "/opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi" 2 # 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Grad.incl" 1 ;Grad2.incl - include file for Gradient Spectroscopy ; for 1 ; ;avance-version (07/01/17) ; ;$CLASS=HighRes Incl ;$COMMENT= define list EA= ;$Id: Grad2.incl,v 1.12 2007/01/22 14:22:35 ber Exp $ # 26 "/opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi" 2 # 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Delay.incl" 1 ;Delay.incl - include file for commonly used delays ; ;version 00/02/07 ; ;$CLASS=HighRes Incl ;$COMMENT= ;general delays define delay DELTA define delay DELTA1 define delay DELTA2 define delay DELTA3 define delay DELTA4 define delay DELTA5 define delay DELTA6 define delay DELTA7 define delay DELTA8 define delay TAU define delay TAU1 define delay TAU2 define delay TAU3 define delay TAU4 define delay TAU5 ;delays for centering pulses define delay CEN_HN1 define delay CEN_HN2 define delay CEN_HN3 define delay CEN_HC1 define delay CEN_HC2 define delay CEN_HC3 define delay CEN_HC4 define delay CEN_HP1 define delay CEN_HP2 define delay CEN_CN1 define delay CEN_CN2 define delay CEN_CN3 define delay CEN_CN4 define delay CEN_CP1 define delay CEN_CP2 ;loop counters define loopcounter COUNTER define loopcounter SCALEF define loopcounter FACTOR1 define loopcounter FACTOR2 define loopcounter FACTOR3 ;$Id: Delay.incl,v 1.12 2005/11/10 12:16:58 ber Exp $ # 27 "/opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi" 2 "p2=p1*2" "p22=p21*2" "d11=30m" "d26=1s/(cnst4*4)" "d0=6u" "in0=inf1/2" "DELTA1=d26-p16-d16-4u" "DELTA2=d26-p1-p16-d16-4u" "DELTA3=p19+d16+8u" "DELTA=d0*2+p14+p21*4/3.1416+8u" "l0=1" # 1 "mc_line 55 file /opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi expanding definition part of mc command before ze" define delay MCWRK define delay MCREST define loopcounter ST1CNT "ST1CNT = td1 / (2)" "MCWRK = 0.111111*d11" "MCREST = d11 - d11" dccorr # 55 "/opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi" 1 ze # 1 "mc_line 55 file /opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi expanding definition of mc command after ze" # 56 "/opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi" # 1 "mc_line 56 file /opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi expanding start label for mc command" 2 MCWRK * 4 LBLSTS1, MCWRK * 4 LBLF1, MCWRK MCREST # 57 "/opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi" 3 d1 pl1:f1 50u setnmr3|0 setnmr0|34|32|33 ctrlgrad 0 (p1 ph1) 4u p16:gp1 d16 DELTA1 (center (p2 ph2) (p22 ph1):f3 ) 4u DELTA1 p16:gp1 d16 (p1 ph3) if "l0 %2 == 1" { (p21 ph4):f3 } else { (p21 ph5):f3 } d0 gron0 2u groff (p14:sp3 ph1):f2 d0 gron0*-1 2u groff 4u p19:gp2*EA d16 (p22 ph1):f3 DELTA p19:gp2*-1*EA d16 (p1 ph6) 4u p16:gp3 d16 DELTA2 pl1:f1 (center (p2 ph1) (p22 ph1):f3 ) 4u DELTA1 p16:gp3 d16 (center (p1 ph1) (p21 ph2):f3 ) 4u p16:gp4 d16 DELTA1 (center (p2 ph1) (p22 ph1):f3 ) DELTA1 p16:gp4 d16 4u (p21 ph7):f3 DELTA3 (p2 ph1) 4u p19:gp5 d16 4u setnmr3^0 setnmr0^34^32^33 ctrlgrad 7 go=2 ph31 # 1 "mc_line 133 file /opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi expanding mc command in line" MCWRK wr #0 if #0 zd igrad EA MCWRK ip6*2 MCWRK ip7*2 MCWRK iu0 lo to LBLSTS1 times 2 MCWRK id0 MCWRK ip4*2 MCWRK ip5*2 MCWRK ip31*2 lo to LBLF1 times ST1CNT MCWRK # 135 "/opt/topspin2.1/exp/stan/nmr/lists/pp/trosyetf3gpsi" exit ph1=0 ph2=1 ph3=3 ph4=1 3 2 0 ph5=3 1 2 0 ph6=1 ph7=0 ph31=0 2 3 1 ;pl1 : f1 channel - power level for pulse (default) ;pl3 : f3 channel - power level for pulse (default) ;sp3: f2 channel - shaped pulse 180 degree (adiabatic) ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p14: f2 channel - 180 degree shaped pulse for inversion (adiabatic) ;p16: homospoil/gradient pulse [1 msec] ;p19: gradient pulse 2 [500 usec] ;p21: f3 channel - 90 degree high power pulse ;p22: f3 channel - 180 degree high power pulse ;d0 : incremented delay (2D) [6 usec] ;d1 : relaxation delay; 1-5 * T1 ;d11: delay for disk I/O [30 msec] ;d16: delay for homospoil/gradient recovery ;d26 : 1/(4J)YH ;cnst4: = J(YH) ;inf1: 1/SW(X) = 2 * DW(X) ;in0: 1/(2 * SW(X)) = DW(X) ;nd0: 2 ;NS: 4 * n ;DS: 16 ;td1: number of experiments ;FnMODE: echo-antiecho ;use gradient ratio: gp 0 : gp 1 : gp 2 : gp 3 : gp 4 : gp 5 ; 3 : 30 : 80 : 45 : 50 : 16.2 ;for z-only gradients: ;gpz0: 3% ;gpz1: 30% ;gpz2: 80% ;gpz3: 45% ;gpz4: 50% ;gpz5: 16.2% ;use gradient files: ;gpnam1: SINE.100 ;gpnam2: SINE.50 ;gpnam3: SINE.100 ;gpnam4: SINE.100 ;gpnam5: SINE.50 ;preprocessor-flags-start ;1: for C-13 and N-15 labeled samples start experiment with ; option -DLABEL_CN (eda: ZGOPTNS) ;preprocessor-flags-end ;$Id: trosyetf3gpsi,v 1.7 2007/04/11 13:34:31 ber Exp $