# 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi"
;hsqcetgpsi
;avance-version (07/04/04)
;HSQC
;2D H-1/X correlation via double inept transfer
; using sensitivity improvement
;phase sensitive using Echo/Antiecho-TPPI gradient selection
;with decoupling during acquisition
;using trim pulses in inept transfer
;
;A.G. Palmer III, J. Cavanagh, P.E. Wright & M. Rance, J. Magn.
; Reson. 93, 151-170 (1991)
;L.E. Kay, P. Keifer & T. Saarinen, J. Am. Chem. Soc. 114,
; 10663-5 (1992)
;J. Schleucher, M. Schwendinger, M. Sattler, P. Schmidt, O. Schedletzky,
; S.J. Glaser, O.W. Sorensen & C. Griesinger, J. Biomol. NMR 4,
; 301-306 (1994)
;
;$CLASS=HighRes
;$DIM=2D
;$TYPE=
;$SUBTYPE=
;$COMMENT=
# 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Avance.incl" 1
;Avance2.incl
; for 1
;
;avance-version (07/08/21)
;
;$CLASS=HighRes Incl
;$COMMENT=
;$Id: Avance2.incl,v 1.17.2.1 2007/09/14 16:17:35 ber Exp $
# 26 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi" 2
# 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Grad.incl" 1
;Grad2.incl - include file for Gradient Spectroscopy
; for 1
;
;avance-version (07/01/17)
;
;$CLASS=HighRes Incl
;$COMMENT=
define list<gradient> EA=<EA>
;$Id: Grad2.incl,v 1.12 2007/01/22 14:22:35 ber Exp $
# 27 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi" 2
# 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Delay.incl" 1
;Delay.incl - include file for commonly used delays
;
;version 00/02/07
;
;$CLASS=HighRes Incl
;$COMMENT=
;general delays
define delay DELTA
define delay DELTA1
define delay DELTA2
define delay DELTA3
define delay DELTA4
define delay DELTA5
define delay DELTA6
define delay DELTA7
define delay DELTA8
define delay TAU
define delay TAU1
define delay TAU2
define delay TAU3
define delay TAU4
define delay TAU5
;delays for centering pulses
define delay CEN_HN1
define delay CEN_HN2
define delay CEN_HN3
define delay CEN_HC1
define delay CEN_HC2
define delay CEN_HC3
define delay CEN_HC4
define delay CEN_HP1
define delay CEN_HP2
define delay CEN_CN1
define delay CEN_CN2
define delay CEN_CN3
define delay CEN_CN4
define delay CEN_CP1
define delay CEN_CP2
;loop counters
define loopcounter COUNTER
define loopcounter SCALEF
define loopcounter FACTOR1
define loopcounter FACTOR2
define loopcounter FACTOR3
;$Id: Delay.incl,v 1.12 2005/11/10 12:16:58 ber Exp $
# 28 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi" 2
"p2=p1*2"
"p4=p3*2"
"d4=1s/(cnst2*4)"
"d11=30m"
"d13=4u"
"p22=p21*2"
"d0=3u"
"in0=inf1/2"
"DELTA1=d13+p16+d16+4u"
"DELTA=p16+d16+50u+larger(p2,p22)+d0*2"
# 1 "mc_line 57 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi expanding definition part of mc command before ze"
define delay MCWRK
define delay MCREST
define loopcounter ST1CNT
"ST1CNT = td1 / (2)"
"MCWRK = 0.142857*d1"
"MCREST = d1 - d1"
dccorr
# 57 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi"
1 ze
# 1 "mc_line 57 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi expanding definition of mc command after ze"
# 58 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi"
d11 pl12:f2
# 1 "mc_line 59 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi expanding start label for mc command"
2 MCWRK * 2 do:f2
LBLSTS1, MCWRK * 4
LBLF1, MCWRK
MCREST
# 60 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi"
3 (p1 ph1)
d4 pl2:f2
(center (p2 ph1) (p4 ph6):f2 )
d4
p28 ph1
d13
(p1 ph2) (p3 ph3):f2
d0
(center (p2 ph7) (p22 ph1):f3 )
d0
50u setnmr3|0 setnmr0|34|32|33 ctrlgrad 0
p16:gp1*EA
d16
(p4 ph4):f2
DELTA
(center (p1 ph1) (p3 ph4):f2 )
d24
(center (p2 ph1) (p4 ph1):f2 )
d24
(center (p1 ph2) (p3 ph5):f2 )
d4
(center (p2 ph1) (p4 ph1):f2 )
d4
(p1 ph1)
DELTA1
(p2 ph1)
d13
p16:gp2
d16 pl12:f2
4u setnmr3^0 setnmr0^34^32^33 ctrlgrad 7
go=2 ph31 cpd2:f2
# 1 "mc_line 97 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi expanding mc command in line"
MCWRK do:f2 wr #0 if #0 zd igrad EA MCWRK ip5*2
lo to LBLSTS1 times 2
MCWRK id0 MCWRK ip3*2 MCWRK ip6*2 MCWRK ip31*2
lo to LBLF1 times ST1CNT
MCWRK
# 99 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetgpsi"
exit
ph1=0
ph2=1
ph3=0 2
ph4=0 0 2 2
ph5=1 1 3 3
ph6=0
ph7=0 0 2 2
ph31=0 2 2 0
;pl1 : f1 channel - power level for pulse (default)
;pl2 : f2 channel - power level for pulse (default)
;pl3 : f3 channel - power level for pulse (default)
;pl12: f2 channel - power level for CPD/BB decoupling
;p1 : f1 channel - 90 degree high power pulse
;p2 : f1 channel - 180 degree high power pulse
;p3 : f2 channel - 90 degree high power pulse
;p4 : f2 channel - 180 degree high power pulse
;p16: homospoil/gradient pulse
;p22: f3 channel - 180 degree high power pulse
;p28: f1 channel - trim pulse
;d0 : incremented delay (2D) [3 usec]
;d1 : relaxation delay; 1-5 * T1
;d4 : 1/(4J)XH
;d11: delay for disk I/O [30 msec]
;d13: short delay [4 usec]
;d16: delay for homospoil/gradient recovery
;d24: 1/(8J)XH for all multiplicities
; 1/(4J)XH for XH
;cnst2: = J(XH)
;inf1: 1/SW(X) = 2 * DW(X)
;in0: 1/(2 * SW(X)) = DW(X)
;nd0: 2
;NS: 1 * n
;DS: >= 16
;td1: number of experiments
;FnMODE: echo-antiecho
;cpd2: decoupling according to sequence defined by cpdprg2
;pcpd2: f2 channel - 90 degree pulse for decoupling sequence
;use gradient ratio: gp 1 : gp 2
; 80 : 20.1 for C-13
; 80 : 8.1 for N-15
;for z-only gradients:
;gpz1: 80%
;gpz2: 20.1% for C-13, 8.1% for N-15
;use gradient files:
;gpnam1: SINE.100
;gpnam2: SINE.100
;preprocessor-flags-start
;1: for C-13 and N-15 labeled samples start experiment with
; option -DLABEL_CN (eda: ZGOPTNS)
;preprocessor-flags-end
;$Id: hsqcetgpsi,v 1.5 2007/04/11 13:34:30 ber Exp $