# 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi"
;hsqcetf3gpsi
;avance-version (07/04/04)
;HSQC
;2D H-1/X correlation via double inept transfer
; using sensitivity improvement
;phase sensitive using Echo/Antiecho-TPPI gradient selection
;with decoupling during acquisition
;using trim pulses in inept transfer
;using f3 - channel
;
;A.G. Palmer III, J. Cavanagh, P.E. Wright & M. Rance, J. Magn.
; Reson. 93, 151-170 (1991)
;L.E. Kay, P. Keifer & T. Saarinen, J. Am. Chem. Soc. 114,
; 10663-5 (1992)
;J. Schleucher, M. Schwendinger, M. Sattler, P. Schmidt, O. Schedletzky,
; S.J. Glaser, O.W. Sorensen & C. Griesinger, J. Biomol. NMR 4,
; 301-306 (1994)
;
;$CLASS=HighRes
;$DIM=2D
;$TYPE=
;$SUBTYPE=
;$COMMENT=
# 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Avance.incl" 1
;Avance2.incl
; for 1
;
;avance-version (07/08/21)
;
;$CLASS=HighRes Incl
;$COMMENT=
;$Id: Avance2.incl,v 1.17.2.1 2007/09/14 16:17:35 ber Exp $
# 27 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi" 2
# 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Grad.incl" 1
;Grad2.incl - include file for Gradient Spectroscopy
; for 1
;
;avance-version (07/01/17)
;
;$CLASS=HighRes Incl
;$COMMENT=
define list<gradient> EA=<EA>
;$Id: Grad2.incl,v 1.12 2007/01/22 14:22:35 ber Exp $
# 28 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi" 2
# 1 "/opt/topspin2.1/exp/stan/nmr/lists/pp/Delay.incl" 1
;Delay.incl - include file for commonly used delays
;
;version 00/02/07
;
;$CLASS=HighRes Incl
;$COMMENT=
;general delays
define delay DELTA
define delay DELTA1
define delay DELTA2
define delay DELTA3
define delay DELTA4
define delay DELTA5
define delay DELTA6
define delay DELTA7
define delay DELTA8
define delay TAU
define delay TAU1
define delay TAU2
define delay TAU3
define delay TAU4
define delay TAU5
;delays for centering pulses
define delay CEN_HN1
define delay CEN_HN2
define delay CEN_HN3
define delay CEN_HC1
define delay CEN_HC2
define delay CEN_HC3
define delay CEN_HC4
define delay CEN_HP1
define delay CEN_HP2
define delay CEN_CN1
define delay CEN_CN2
define delay CEN_CN3
define delay CEN_CN4
define delay CEN_CP1
define delay CEN_CP2
;loop counters
define loopcounter COUNTER
define loopcounter SCALEF
define loopcounter FACTOR1
define loopcounter FACTOR2
define loopcounter FACTOR3
;$Id: Delay.incl,v 1.12 2005/11/10 12:16:58 ber Exp $
# 29 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi" 2
"p2=p1*2"
"p22=p21*2"
"d11=30m"
"d13=4u"
"d26=1s/(cnst4*4)"
"d0=3u"
"in0=inf1/2"
"DELTA1=d13+p16+d16+4u"
"DELTA=p16+d16+larger(p2,p14)+d0*2"
# 1 "mc_line 53 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi expanding definition part of mc command before ze"
define delay MCWRK
define delay MCREST
define loopcounter ST1CNT
"ST1CNT = td1 / (2)"
"MCWRK = 0.142857*d1"
"MCREST = d1 - d1"
dccorr
# 53 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi"
1 ze
# 1 "mc_line 53 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi expanding definition of mc command after ze"
# 54 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi"
d11 pl16:f3
# 1 "mc_line 55 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi expanding start label for mc command"
2 MCWRK * 2 do:f3
LBLSTS1, MCWRK * 4
LBLF1, MCWRK
MCREST
# 56 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi"
3 (p1 ph1)
d26 pl3:f3
(center (p2 ph1) (p22 ph6):f3 )
d26 setnmr3|0 setnmr0|34|32|33 ctrlgrad 0
p28 ph1
d13
(p1 ph2)
3u
p16:gp1
d16
(p21 ph3):f3
d0
(center (p2 ph7) (p14:sp3 ph1):f2 )
d0
p16:gp2*EA
d16
(p22 ph4):f3
DELTA
(center (p1 ph1) (p21 ph4):f3 )
d24
(center (p2 ph1) (p22 ph1):f3 )
d24
(center (p1 ph2) (p21 ph5):f3 )
d26
(center (p2 ph1) (p22 ph1):f3 )
d26
(p1 ph1)
DELTA1
(p2 ph1)
d13
p16:gp3
d16 pl16:f3
4u setnmr3^0 setnmr0^34^32^33 ctrlgrad 7
go=2 ph31 cpd3:f3
# 1 "mc_line 96 file /opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi expanding mc command in line"
MCWRK do:f3 wr #0 if #0 zd igrad EA MCWRK ip5*2
lo to LBLSTS1 times 2
MCWRK id0 MCWRK ip3*2 MCWRK ip6*2 MCWRK ip31*2
lo to LBLF1 times ST1CNT
MCWRK
# 98 "/opt/topspin2.1/exp/stan/nmr/lists/pp/hsqcetf3gpsi"
exit
ph1=0
ph2=1
ph3=0 2
ph4=0 0 2 2
ph5=1 1 3 3
ph6=0
ph7=0 0 2 2
ph31=0 2 2 0
;pl1 : f1 channel - power level for pulse (default)
;pl3 : f3 channel - power level for pulse (default)
;pl16: f3 channel - power level for CPD/BB decoupling
;sp3: f2 channel - shaped pulse 180 degree (adiabatic)
;p1 : f1 channel - 90 degree high power pulse
;p2 : f1 channel - 180 degree high power pulse
;p14: f2 channel - 180 degree shaped pulse for inversion (adiabatic)
;p16: homospoil/gradient pulse [1 msec]
;p21: f3 channel - 90 degree high power pulse
;p22: f3 channel - 180 degree high power pulse
;p28: f1 channel - trim pulse [1 msec]
;d0 : incremented delay (2D) [3 usec]
;d1 : relaxation delay; 1-5 * T1
;d11: delay for disk I/O [30 msec]
;d13: short delay [4 usec]
;d16: delay for homospoil/gradient recovery
;d24: 1/(4J)YH for YH
; 1/(8J)YH for all multiplicities
;d26: 1/(4J(YH))
;cnst4: = J(YH)
;inf1: 1/SW(X) = 2 * DW(X)
;in0: 1/(2 * SW(X)) = DW(X)
;nd0: 2
;NS: 1 * n
;DS: >= 16
;td1: number of experiments
;FnMODE: echo-antiecho
;cpd3: decoupling according to sequence defined by cpdprg3
;pcpd3: f3 channel - 90 degree pulse for decoupling sequence
;use gradient ratio: gp 1 : gp 2 : gp 3
; 50 : 80 : 20.1 for C-13
; 50 : 80 : 8.1 for N-15
;for z-only gradients:
;gpz1: 50%
;gpz2: 80%
;gpz3: 20.1% for C-13, 8.1% for N-15
;use gradient files:
;gpnam1: SINE.100
;gpnam2: SINE.100
;gpnam3: SINE.100
;preprocessor-flags-start
;1: for C-13 and N-15 labeled samples start experiment with
; option -DLABEL_CN (eda: ZGOPTNS)
;preprocessor-flags-end
;$Id: hsqcetf3gpsi,v 1.4 2007/04/11 13:34:30 ber Exp $