data_1QZA
# 
_entry.id   1QZA 
# 
_audit_conform.dict_name       mmcif_pdbx.dic 
_audit_conform.dict_version    4.031 
_audit_conform.dict_location   http://mmcif.pdb.org/dictionaries/ascii/mmcif_pdbx.dic 
# 
loop_
_database_2.database_id 
_database_2.database_code 
PDB  1QZA       
RCSB RCSB020251 
# 
loop_
_database_PDB_rev.num 
_database_PDB_rev.date 
_database_PDB_rev.date_original 
_database_PDB_rev.status 
_database_PDB_rev.replaces 
_database_PDB_rev.mod_type 
1 2003-11-04 2003-09-16 ? 1QZA 0 
2 2009-02-24 ?          ? 1QZA 1 
# 
_database_PDB_rev_record.rev_num   2 
_database_PDB_rev_record.type      VERSN 
_database_PDB_rev_record.details   ? 
# 
loop_
_pdbx_database_related.db_name 
_pdbx_database_related.db_id 
_pdbx_database_related.details 
_pdbx_database_related.content_type 
PDB  1OB2     'E. coli elongation factor EF-Tu complexed with the antibiotic kirromycin, a GTP analog, and Phe-tRNA' unspecified 
PDB  1QZB     'Coordinates of the A-site tRNA model fitted into the cryo-EM map of 70S ribosome in the pre-translocational state' 
unspecified            
PDB  1QZC     
;Coordinates of S12, SH44, LH69 and SRL separately fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin)bound 70S ribosome
;
unspecified            
PDB  1QZD     
'EF-Tu.kirromycin coordinates fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin)bound 70S ribosome' unspecified 
PDB  1R2W     'COORDINATES OF L11 WITH 58NTS OF 23S RRNA FITTED INTO THE CRYO-EM MAP OF THE 70S RIBOSOME' unspecified            
PDB  1R2X     
'COORDINATES OF L11 WITH 58NTS OF 23S RRNA FITTED INTO THE CRYO-EM MAP OF EF-TU TERNARY COMPLEX (GDP.KIRROMYCIN) BOUND 70S RIBOSOME' 
unspecified            
EMDB EMD-1055 . 'associated EM volume' 
# 
_pdbx_database_status.status_code      REL 
_pdbx_database_status.entry_id         1QZA 
_pdbx_database_status.deposit_site     RCSB 
_pdbx_database_status.process_site     RCSB 
_pdbx_database_status.SG_entry         . 
_pdbx_database_status.status_code_sf   ? 
_pdbx_database_status.status_code_mr   ? 
# 
loop_
_audit_author.name 
_audit_author.pdbx_ordinal 
'Valle, M.'     1  
'Zavialov, A.'  2  
'Li, W.'        3  
'Stagg, S.M.'   4  
'Sengupta, J.'  5  
'Nielsen, R.C.' 6  
'Nissen, P.'    7  
'Harvey, S.C.'  8  
'Ehrenberg, M.' 9  
'Frank, J.'     10 
# 
_citation.id                        primary 
_citation.title                     'Incorporation of Aminoacyl-tRNA into the Ribosome as seen by Cryo-electron Microscopy' 
_citation.journal_abbrev            Nat.Struct.Biol. 
_citation.journal_volume            10 
_citation.page_first                899 
_citation.page_last                 906 
_citation.year                      2003 
_citation.journal_id_ASTM           NSBIEW 
_citation.country                   US 
_citation.journal_id_ISSN           1072-8368 
_citation.journal_id_CSD            2024 
_citation.book_publisher            ? 
_citation.pdbx_database_id_PubMed   14566331 
_citation.pdbx_database_id_DOI      10.1038/nsb1003 
# 
loop_
_citation_author.citation_id 
_citation_author.name 
_citation_author.ordinal 
primary 'Valle, M.'     1  
primary 'Zavialov, A.'  2  
primary 'Li, W.'        3  
primary 'Stagg, S.M.'   4  
primary 'Sengupta, J.'  5  
primary 'Nielsen, R.C.' 6  
primary 'Nissen, P.'    7  
primary 'Harvey, S.C.'  8  
primary 'Ehrenberg, M.' 9  
primary 'Frank, J.'     10 
# 
_cell.entry_id           1QZA 
_cell.length_a           1 
_cell.length_b           1 
_cell.length_c           1 
_cell.angle_alpha        90 
_cell.angle_beta         90 
_cell.angle_gamma        90 
_cell.pdbx_unique_axis   ? 
_cell.Z_PDB              1 
_cell.length_a_esd       ? 
_cell.length_b_esd       ? 
_cell.length_c_esd       ? 
_cell.angle_alpha_esd    ? 
_cell.angle_beta_esd     ? 
_cell.angle_gamma_esd    ? 
# 
_symmetry.entry_id                         1QZA 
_symmetry.space_group_name_H-M             'P 1' 
_symmetry.pdbx_full_space_group_name_H-M   ? 
_symmetry.Int_Tables_number                ? 
_symmetry.cell_setting                     ? 
# 
_entity.id                         1 
_entity.type                       polymer 
_entity.src_method                 syn 
_entity.pdbx_description           Phe-tRNA 
_entity.formula_weight             24189.523 
_entity.pdbx_number_of_molecules   1 
_entity.details                    ? 
# 
_entity_poly.entity_id                      1 
_entity_poly.type                           polyribonucleotide 
_entity_poly.nstd_linkage                   no 
_entity_poly.nstd_monomer                   no 
_entity_poly.pdbx_seq_one_letter_code       GCGGAUUUAGCUCAGUUGGGAGAGCGCCAGACUGAAGAUCUGGAGGUCCUGUGUUCGAUCCACAGAAUUCGCACC 
_entity_poly.pdbx_seq_one_letter_code_can   GCGGAUUUAGCUCAGUUGGGAGAGCGCCAGACUGAAGAUCUGGAGGUCCUGUGUUCGAUCCACAGAAUUCGCACC 
_entity_poly.pdbx_strand_id                 B 
# 
loop_
_entity_poly_seq.entity_id 
_entity_poly_seq.num 
_entity_poly_seq.mon_id 
_entity_poly_seq.hetero 
1 1  G n 
1 2  C n 
1 3  G n 
1 4  G n 
1 5  A n 
1 6  U n 
1 7  U n 
1 8  U n 
1 9  A n 
1 10 G n 
1 11 C n 
1 12 U n 
1 13 C n 
1 14 A n 
1 15 G n 
1 16 U n 
1 17 U n 
1 18 G n 
1 19 G n 
1 20 G n 
1 21 A n 
1 22 G n 
1 23 A n 
1 24 G n 
1 25 C n 
1 26 G n 
1 27 C n 
1 28 C n 
1 29 A n 
1 30 G n 
1 31 A n 
1 32 C n 
1 33 U n 
1 34 G n 
1 35 A n 
1 36 A n 
1 37 G n 
1 38 A n 
1 39 U n 
1 40 C n 
1 41 U n 
1 42 G n 
1 43 G n 
1 44 A n 
1 45 G n 
1 46 G n 
1 47 U n 
1 48 C n 
1 49 C n 
1 50 U n 
1 51 G n 
1 52 U n 
1 53 G n 
1 54 U n 
1 55 U n 
1 56 C n 
1 57 G n 
1 58 A n 
1 59 U n 
1 60 C n 
1 61 C n 
1 62 A n 
1 63 C n 
1 64 A n 
1 65 G n 
1 66 A n 
1 67 A n 
1 68 U n 
1 69 U n 
1 70 C n 
1 71 G n 
1 72 C n 
1 73 A n 
1 74 C n 
1 75 C n 
# 
_struct_ref.id                  1 
_struct_ref.entity_id           1 
_struct_ref.db_name             PDB 
_struct_ref.db_code             1QZA 
_struct_ref.pdbx_db_accession   1QZA 
_struct_ref.biol_id             . 
# 
_struct_ref_seq.align_id                      1 
_struct_ref_seq.ref_id                        1 
_struct_ref_seq.pdbx_PDB_id_code              1QZA 
_struct_ref_seq.pdbx_strand_id                B 
_struct_ref_seq.seq_align_beg                 1 
_struct_ref_seq.pdbx_seq_align_beg_ins_code   ? 
_struct_ref_seq.seq_align_end                 75 
_struct_ref_seq.pdbx_seq_align_end_ins_code   ? 
_struct_ref_seq.pdbx_db_accession             1QZA 
_struct_ref_seq.db_align_beg                  1 
_struct_ref_seq.db_align_end                  75 
_struct_ref_seq.pdbx_auth_seq_align_beg       1 
_struct_ref_seq.pdbx_auth_seq_align_end       75 
# 
loop_
_chem_comp.id 
_chem_comp.type 
_chem_comp.mon_nstd_flag 
_chem_comp.name 
_chem_comp.pdbx_synonyms 
_chem_comp.formula 
_chem_comp.formula_weight 
G 'RNA linking' y "GUANOSINE-5'-MONOPHOSPHATE" ? 'C10 H14 N5 O8 P' 363.223 
C 'RNA linking' y "CYTIDINE-5'-MONOPHOSPHATE"  ? 'C9 H14 N3 O8 P'  323.199 
A 'RNA linking' y "ADENOSINE-5'-MONOPHOSPHATE" ? 'C10 H14 N5 O7 P' 347.224 
U 'RNA linking' y "URIDINE-5'-MONOPHOSPHATE"   ? 'C9 H13 N2 O9 P'  324.183 
# 
_exptl.entry_id          1QZA 
_exptl.method            'ELECTRON MICROSCOPY' 
_exptl.crystals_number   ? 
# 
_exptl_crystal.id                    1 
_exptl_crystal.density_meas          ? 
_exptl_crystal.density_Matthews      ? 
_exptl_crystal.density_percent_sol   ? 
_exptl_crystal.description           ? 
# 
_refine_hist.pdbx_refine_id                   'ELECTRON MICROSCOPY' 
_refine_hist.cycle_id                         LAST 
_refine_hist.pdbx_number_atoms_protein        0 
_refine_hist.pdbx_number_atoms_nucleic_acid   75 
_refine_hist.pdbx_number_atoms_ligand         0 
_refine_hist.number_atoms_solvent             0 
_refine_hist.number_atoms_total               75 
_refine_hist.d_res_high                       . 
_refine_hist.d_res_low                        . 
# 
_struct.entry_id                  1QZA 
_struct.title                     
'Coordinates of the A/T site tRNA model fitted into the cryo-EM map of EF-Tu ternary complex (GDP.Kirromycin) bound 70S ribosome' 
_struct.pdbx_descriptor           Phe-tRNA 
_struct.pdbx_model_details        ? 
_struct.pdbx_CASP_flag            ? 
_struct.pdbx_model_type_details   ? 
# 
_struct_keywords.entry_id        1QZA 
_struct_keywords.pdbx_keywords   RNA 
_struct_keywords.text            'tRNA model, decoding, A/T-site tRNA., RNA' 
# 
_struct_asym.id                            A 
_struct_asym.pdbx_blank_PDB_chainid_flag   N 
_struct_asym.pdbx_modified                 N 
_struct_asym.entity_id                     1 
_struct_asym.details                       ? 
# 
_struct_biol.id                    1 
_struct_biol.pdbx_parent_biol_id   ? 
_struct_biol.details               ? 
# 
_database_PDB_matrix.entry_id          1QZA 
_database_PDB_matrix.origx[1][1]       1.000000 
_database_PDB_matrix.origx[1][2]       0.000000 
_database_PDB_matrix.origx[1][3]       0.000000 
_database_PDB_matrix.origx[2][1]       0.000000 
_database_PDB_matrix.origx[2][2]       1.000000 
_database_PDB_matrix.origx[2][3]       0.000000 
_database_PDB_matrix.origx[3][1]       0.000000 
_database_PDB_matrix.origx[3][2]       0.000000 
_database_PDB_matrix.origx[3][3]       1.000000 
_database_PDB_matrix.origx_vector[1]   0.00000 
_database_PDB_matrix.origx_vector[2]   0.00000 
_database_PDB_matrix.origx_vector[3]   0.00000 
# 
_atom_sites.entry_id                    1QZA 
_atom_sites.Cartn_transform_axes        ? 
_atom_sites.fract_transf_matrix[1][1]   1.000000 
_atom_sites.fract_transf_matrix[1][2]   0.000000 
_atom_sites.fract_transf_matrix[1][3]   0.000000 
_atom_sites.fract_transf_matrix[2][1]   0.000000 
_atom_sites.fract_transf_matrix[2][2]   1.000000 
_atom_sites.fract_transf_matrix[2][3]   0.000000 
_atom_sites.fract_transf_matrix[3][1]   0.000000 
_atom_sites.fract_transf_matrix[3][2]   0.000000 
_atom_sites.fract_transf_matrix[3][3]   1.000000 
_atom_sites.fract_transf_vector[1]      0.00000 
_atom_sites.fract_transf_vector[2]      0.00000 
_atom_sites.fract_transf_vector[3]      0.00000 
# 
_atom_type.symbol   P 
# 
loop_
_atom_site.group_PDB 
_atom_site.id 
_atom_site.type_symbol 
_atom_site.label_atom_id 
_atom_site.label_alt_id 
_atom_site.label_comp_id 
_atom_site.label_asym_id 
_atom_site.label_entity_id 
_atom_site.label_seq_id 
_atom_site.pdbx_PDB_ins_code 
_atom_site.Cartn_x 
_atom_site.Cartn_y 
_atom_site.Cartn_z 
_atom_site.occupancy 
_atom_site.B_iso_or_equiv 
_atom_site.Cartn_x_esd 
_atom_site.Cartn_y_esd 
_atom_site.Cartn_z_esd 
_atom_site.occupancy_esd 
_atom_site.B_iso_or_equiv_esd 
_atom_site.pdbx_formal_charge 
_atom_site.auth_seq_id 
_atom_site.auth_comp_id 
_atom_site.auth_asym_id 
_atom_site.auth_atom_id 
_atom_site.pdbx_PDB_model_num 
ATOM 1  P P . G A 1 1  ? -24.604 51.452 -47.785 1.00 37.10 ? ? ? ? ? ? 1  G B P 1 
ATOM 2  P P . C A 1 2  ? -22.560 47.565 -50.981 1.00 0.00  ? ? ? ? ? ? 2  C B P 1 
ATOM 3  P P . G A 1 3  ? -19.739 42.120 -50.271 1.00 0.00  ? ? ? ? ? ? 3  G B P 1 
ATOM 4  P P . G A 1 4  ? -19.552 36.440 -46.055 1.00 0.00  ? ? ? ? ? ? 4  G B P 1 
ATOM 5  P P . A A 1 5  ? -21.173 32.188 -41.018 1.00 0.00  ? ? ? ? ? ? 5  A B P 1 
ATOM 6  P P . U A 1 6  ? -24.381 29.372 -36.707 1.00 0.00  ? ? ? ? ? ? 6  U B P 1 
ATOM 7  P P . U A 1 7  ? -29.927 27.806 -34.668 1.00 0.00  ? ? ? ? ? ? 7  U B P 1 
ATOM 8  P P . U A 1 8  ? -36.011 27.557 -33.938 1.00 0.00  ? ? ? ? ? ? 8  U B P 1 
ATOM 9  P P . A A 1 9  ? -39.932 25.127 -32.665 1.00 0.00  ? ? ? ? ? ? 9  A B P 1 
ATOM 10 P P . G A 1 10 ? -45.490 23.645 -32.338 1.00 0.00  ? ? ? ? ? ? 10 G B P 1 
ATOM 11 P P . C A 1 11 ? -42.126 27.657 -29.337 1.00 0.00  ? ? ? ? ? ? 11 C B P 1 
ATOM 12 P P . U A 1 12 ? -37.610 28.609 -25.970 1.00 0.00  ? ? ? ? ? ? 12 U B P 1 
ATOM 13 P P . C A 1 13 ? -32.136 26.389 -24.891 1.00 0.00  ? ? ? ? ? ? 13 C B P 1 
ATOM 14 P P . A A 1 14 ? -29.218 21.238 -28.901 1.00 0.00  ? ? ? ? ? ? 14 A B P 1 
ATOM 15 P P . G A 1 15 ? -27.223 15.733 -31.578 1.00 0.00  ? ? ? ? ? ? 15 G B P 1 
ATOM 16 P P . U A 1 16 ? -27.016 12.313 -36.378 1.00 0.00  ? ? ? ? ? ? 16 U B P 1 
ATOM 17 P P . U A 1 17 ? -27.326 11.992 -42.221 1.00 0.00  ? ? ? ? ? ? 17 U B P 1 
ATOM 18 P P . G A 1 18 ? -28.394 14.643 -46.927 1.00 0.00  ? ? ? ? ? ? 18 G B P 1 
ATOM 19 P P . G A 1 19 ? -33.871 11.573 -44.790 1.00 0.00  ? ? ? ? ? ? 19 G B P 1 
ATOM 20 P P . G A 1 20 ? -38.064 6.551  -44.178 1.00 0.00  ? ? ? ? ? ? 20 G B P 1 
ATOM 21 P P . A A 1 21 ? -41.717 11.613 -41.541 1.00 0.00  ? ? ? ? ? ? 21 A B P 1 
ATOM 22 P P . G A 1 22 ? -42.126 11.634 -34.331 1.00 0.00  ? ? ? ? ? ? 22 G B P 1 
ATOM 23 P P . A A 1 23 ? -40.583 10.555 -27.786 1.00 0.00  ? ? ? ? ? ? 23 A B P 1 
ATOM 24 P P . G A 1 24 ? -39.377 11.135 -22.148 1.00 0.00  ? ? ? ? ? ? 24 G B P 1 
ATOM 25 P P . C A 1 25 ? -40.644 15.200 -17.716 1.00 0.00  ? ? ? ? ? ? 25 C B P 1 
ATOM 26 P P . G A 1 26 ? -43.855 19.874 -15.996 1.00 0.00  ? ? ? ? ? ? 26 G B P 1 
ATOM 27 P P . C A 1 27 ? -49.311 22.625 -17.327 1.00 0.00  ? ? ? ? ? ? 27 C B P 1 
ATOM 28 P P . C A 1 28 ? -53.882 21.353 -21.036 1.00 0.00  ? ? ? ? ? ? 28 C B P 1 
ATOM 29 P P . A A 1 29 ? -58.838 19.067 -23.494 1.00 0.00  ? ? ? ? ? ? 29 A B P 1 
ATOM 30 P P . G A 1 30 ? -63.013 14.641 -22.119 1.00 0.00  ? ? ? ? ? ? 30 G B P 1 
ATOM 31 P P . A A 1 31 ? -66.043 10.988 -18.336 1.00 0.00  ? ? ? ? ? ? 31 A B P 1 
ATOM 32 P P . C A 1 32 ? -66.924 10.799 -14.263 1.00 0.00  ? ? ? ? ? ? 32 C B P 1 
ATOM 33 P P . U A 1 33 ? -67.867 13.863 -8.776  1.00 0.00  ? ? ? ? ? ? 33 U B P 1 
ATOM 34 P P . G A 1 34 ? -67.177 17.903 -6.064  1.00 0.00  ? ? ? ? ? ? 34 G B P 1 
ATOM 35 P P . A A 1 35 ? -63.937 19.519 -10.485 1.00 0.00  ? ? ? ? ? ? 35 A B P 1 
ATOM 36 P P . A A 1 36 ? -57.948 19.157 -11.951 1.00 0.00  ? ? ? ? ? ? 36 A B P 1 
ATOM 37 P P . G A 1 37 ? -52.379 16.963 -10.622 1.00 0.00  ? ? ? ? ? ? 37 G B P 1 
ATOM 38 P P . A A 1 38 ? -49.474 12.375 -7.984  1.00 0.00  ? ? ? ? ? ? 38 A B P 1 
ATOM 39 P P . U A 1 39 ? -50.202 6.736  -7.874  1.00 0.00  ? ? ? ? ? ? 39 U B P 1 
ATOM 40 P P . C A 1 40 ? -52.368 3.128  -11.870 1.00 0.00  ? ? ? ? ? ? 40 C B P 1 
ATOM 41 P P . U A 1 41 ? -54.242 1.488  -16.381 1.00 0.00  ? ? ? ? ? ? 41 U B P 1 
ATOM 42 P P . G A 1 42 ? -53.534 1.814  -21.904 1.00 0.00  ? ? ? ? ? ? 42 G B P 1 
ATOM 43 P P . G A 1 43 ? -52.210 4.503  -27.390 1.00 0.00  ? ? ? ? ? ? 43 G B P 1 
ATOM 44 P P . A A 1 44 ? -51.312 10.611 -30.869 1.00 0.00  ? ? ? ? ? ? 44 A B P 1 
ATOM 45 P P . G A 1 45 ? -51.605 15.468 -32.986 1.00 0.00  ? ? ? ? ? ? 45 G B P 1 
ATOM 46 P P . G A 1 46 ? -48.719 18.715 -35.203 1.00 0.00  ? ? ? ? ? ? 46 G B P 1 
ATOM 47 P P . U A 1 47 ? -45.894 23.684 -37.084 1.00 0.00  ? ? ? ? ? ? 47 U B P 1 
ATOM 48 P P . C A 1 48 ? -41.348 22.647 -42.021 1.00 0.00  ? ? ? ? ? ? 48 C B P 1 
ATOM 49 P P . C A 1 49 ? -35.673 25.880 -39.819 1.00 0.00  ? ? ? ? ? ? 49 C B P 1 
ATOM 50 P P . U A 1 50 ? -40.688 27.567 -42.595 1.00 0.00  ? ? ? ? ? ? 50 U B P 1 
ATOM 51 P P . G A 1 51 ? -43.258 29.571 -47.495 1.00 0.00  ? ? ? ? ? ? 51 G B P 1 
ATOM 52 P P . U A 1 52 ? -43.475 29.778 -53.229 1.00 0.00  ? ? ? ? ? ? 52 U B P 1 
ATOM 53 P P . G A 1 53 ? -40.169 26.186 -58.189 1.00 0.00  ? ? ? ? ? ? 53 G B P 1 
ATOM 54 P P . U A 1 54 ? -36.416 22.686 -61.345 1.00 0.00  ? ? ? ? ? ? 54 U B P 1 
ATOM 55 P P . U A 1 55 ? -34.002 17.671 -62.012 1.00 0.00  ? ? ? ? ? ? 55 U B P 1 
ATOM 56 P P . C A 1 56 ? -35.642 12.615 -61.499 1.00 0.00  ? ? ? ? ? ? 56 C B P 1 
ATOM 57 P P . G A 1 57 ? -39.689 13.906 -57.695 1.00 0.00  ? ? ? ? ? ? 57 G B P 1 
ATOM 58 P P . A A 1 58 ? -40.338 15.684 -52.607 1.00 0.00  ? ? ? ? ? ? 58 A B P 1 
ATOM 59 P P . U A 1 59 ? -39.405 18.075 -46.936 1.00 0.00  ? ? ? ? ? ? 59 U B P 1 
ATOM 60 P P . C A 1 60 ? -34.663 20.553 -47.045 1.00 0.00  ? ? ? ? ? ? 60 C B P 1 
ATOM 61 P P . C A 1 61 ? -28.255 21.924 -46.596 1.00 0.00  ? ? ? ? ? ? 61 C B P 1 
ATOM 62 P P . A A 1 62 ? -26.057 23.937 -51.197 1.00 0.00  ? ? ? ? ? ? 62 A B P 1 
ATOM 63 P P . C A 1 63 ? -26.114 28.249 -54.910 1.00 0.00  ? ? ? ? ? ? 63 C B P 1 
ATOM 64 P P . A A 1 64 ? -28.534 33.923 -55.347 1.00 0.00  ? ? ? ? ? ? 64 A B P 1 
ATOM 65 P P . G A 1 65 ? -32.342 39.392 -53.123 1.00 0.00  ? ? ? ? ? ? 65 G B P 1 
ATOM 66 P P . A A 1 66 ? -35.304 42.378 -47.724 1.00 0.00  ? ? ? ? ? ? 66 A B P 1 
ATOM 67 P P . A A 1 67 ? -36.429 43.872 -42.221 1.00 0.00  ? ? ? ? ? ? 67 A B P 1 
ATOM 68 P P . U A 1 68 ? -35.061 43.959 -36.052 1.00 0.00  ? ? ? ? ? ? 68 U B P 1 
ATOM 69 P P . U A 1 69 ? -31.431 42.711 -31.457 1.00 0.00  ? ? ? ? ? ? 69 U B P 1 
ATOM 70 P P . C A 1 70 ? -24.485 42.235 -30.365 1.00 0.00  ? ? ? ? ? ? 70 C B P 1 
ATOM 71 P P . G A 1 71 ? -18.649 41.323 -31.441 1.00 0.00  ? ? ? ? ? ? 71 G B P 1 
ATOM 72 P P . C A 1 72 ? -13.515 43.826 -35.551 1.00 0.00  ? ? ? ? ? ? 72 C B P 1 
ATOM 73 P P . A A 1 73 ? -11.378 48.131 -40.556 1.00 0.00  ? ? ? ? ? ? 73 A B P 1 
ATOM 74 P P . C A 1 74 ? -11.518 53.397 -44.780 1.00 0.00  ? ? ? ? ? ? 74 C B P 1 
ATOM 75 P P . C A 1 75 ? -13.042 58.653 -46.853 1.00 0.00  ? ? ? ? ? ? 75 C B P 1 
# 
loop_
_pdbx_poly_seq_scheme.asym_id 
_pdbx_poly_seq_scheme.entity_id 
_pdbx_poly_seq_scheme.seq_id 
_pdbx_poly_seq_scheme.mon_id 
_pdbx_poly_seq_scheme.ndb_seq_num 
_pdbx_poly_seq_scheme.pdb_seq_num 
_pdbx_poly_seq_scheme.auth_seq_num 
_pdbx_poly_seq_scheme.pdb_mon_id 
_pdbx_poly_seq_scheme.auth_mon_id 
_pdbx_poly_seq_scheme.pdb_strand_id 
_pdbx_poly_seq_scheme.pdb_ins_code 
_pdbx_poly_seq_scheme.hetero 
A 1 1  G 1  1  1  G G B . n 
A 1 2  C 2  2  2  C C B . n 
A 1 3  G 3  3  3  G G B . n 
A 1 4  G 4  4  4  G G B . n 
A 1 5  A 5  5  5  A A B . n 
A 1 6  U 6  6  6  U U B . n 
A 1 7  U 7  7  7  U U B . n 
A 1 8  U 8  8  8  U U B . n 
A 1 9  A 9  9  9  A A B . n 
A 1 10 G 10 10 10 G G B . n 
A 1 11 C 11 11 11 C C B . n 
A 1 12 U 12 12 12 U U B . n 
A 1 13 C 13 13 13 C C B . n 
A 1 14 A 14 14 14 A A B . n 
A 1 15 G 15 15 15 G G B . n 
A 1 16 U 16 16 16 U U B . n 
A 1 17 U 17 17 17 U U B . n 
A 1 18 G 18 18 18 G G B . n 
A 1 19 G 19 19 19 G G B . n 
A 1 20 G 20 20 20 G G B . n 
A 1 21 A 21 21 21 A A B . n 
A 1 22 G 22 22 22 G G B . n 
A 1 23 A 23 23 23 A A B . n 
A 1 24 G 24 24 24 G G B . n 
A 1 25 C 25 25 25 C C B . n 
A 1 26 G 26 26 26 G G B . n 
A 1 27 C 27 27 27 C C B . n 
A 1 28 C 28 28 28 C C B . n 
A 1 29 A 29 29 29 A A B . n 
A 1 30 G 30 30 30 G G B . n 
A 1 31 A 31 31 31 A A B . n 
A 1 32 C 32 32 32 C C B . n 
A 1 33 U 33 33 33 U U B . n 
A 1 34 G 34 34 34 G G B . n 
A 1 35 A 35 35 35 A A B . n 
A 1 36 A 36 36 36 A A B . n 
A 1 37 G 37 37 37 G G B . n 
A 1 38 A 38 38 38 A A B . n 
A 1 39 U 39 39 39 U U B . n 
A 1 40 C 40 40 40 C C B . n 
A 1 41 U 41 41 41 U U B . n 
A 1 42 G 42 42 42 G G B . n 
A 1 43 G 43 43 43 G G B . n 
A 1 44 A 44 44 44 A A B . n 
A 1 45 G 45 45 45 G G B . n 
A 1 46 G 46 46 46 G G B . n 
A 1 47 U 47 47 47 U U B . n 
A 1 48 C 48 48 48 C C B . n 
A 1 49 C 49 49 49 C C B . n 
A 1 50 U 50 50 50 U U B . n 
A 1 51 G 51 51 51 G G B . n 
A 1 52 U 52 52 52 U U B . n 
A 1 53 G 53 53 53 G G B . n 
A 1 54 U 54 54 54 U U B . n 
A 1 55 U 55 55 55 U U B . n 
A 1 56 C 56 56 56 C C B . n 
A 1 57 G 57 57 57 G G B . n 
A 1 58 A 58 58 58 A A B . n 
A 1 59 U 59 59 59 U U B . n 
A 1 60 C 60 60 60 C C B . n 
A 1 61 C 61 61 61 C C B . n 
A 1 62 A 62 62 62 A A B . n 
A 1 63 C 63 63 63 C C B . n 
A 1 64 A 64 64 64 A A B . n 
A 1 65 G 65 65 65 G G B . n 
A 1 66 A 66 66 66 A A B . n 
A 1 67 A 67 67 67 A A B . n 
A 1 68 U 68 68 68 U U B . n 
A 1 69 U 69 69 69 U U B . n 
A 1 70 C 70 70 70 C C B . n 
A 1 71 G 71 71 71 G G B . n 
A 1 72 C 72 72 72 C C B . n 
A 1 73 A 73 73 73 A A B . n 
A 1 74 C 74 74 74 C C B . n 
A 1 75 C 75 75 75 C C B . n 
# 
_em_3d_fitting.id                1 
_em_3d_fitting.entry_id          1QZA 
_em_3d_fitting.ref_space         REAL 
_em_3d_fitting.ref_protocol      ? 
_em_3d_fitting.target_criteria   ? 
_em_3d_fitting.overall_b_value   ? 
_em_3d_fitting.method            'Manual fitting in O' 
_em_3d_fitting.software_name     ? 
_em_3d_fitting.details           ? 
# 
_em_3d_fitting_list.id              1 
_em_3d_fitting_list.pdb_entry_id    1OB2 
_em_3d_fitting_list.3d_fitting_id   1 
_em_3d_fitting_list.pdb_chain_id    ? 
# 
_em_3d_reconstruction.entry_id                    1QZA 
_em_3d_reconstruction.id                          1 
_em_3d_reconstruction.method                      '3D projection matching; conjugate gradient with regularization' 
_em_3d_reconstruction.nominal_pixel_size          ? 
_em_3d_reconstruction.actual_pixel_size           2.82 
_em_3d_reconstruction.resolution                  10 
_em_3d_reconstruction.ctf_correction_method       'CTF correction of 3D maps by Wiener filteration' 
_em_3d_reconstruction.magnification_calibration   TMV 
_em_3d_reconstruction.details                     'SPIDER package' 
_em_3d_reconstruction.resolution_method           ? 
_em_3d_reconstruction.citation_id                 ? 
# 
_em_assembly.id                  1 
_em_assembly.entry_id            1QZA 
_em_assembly.aggregation_state   PARTICLE 
_em_assembly.name                ? 
_em_assembly.details             '70S + fMet-tRNA + PhetRNA + EF-Tu + GDP + kir mRNA codes for MP-stop' 
_em_assembly.composition         ? 
_em_assembly.mol_wt_exp          ? 
_em_assembly.mol_wt_method       ? 
_em_assembly.mol_wt_theo         ? 
_em_assembly.num_components      ? 
# 
_em_experiment.entry_id                1QZA 
_em_experiment.reconstruction_method   'SINGLE PARTICLE' 
_em_experiment.specimen_type           'VITREOUS ICE (CRYO EM)' 
# 
_em_single_particle_entity.entry_id        1QZA 
_em_single_particle_entity.symmetry_type   ASYMMETRIC 
# 
_em_buffer.id        1 
_em_buffer.name      'Polymix buffer' 
_em_buffer.details   ? 
# 
_em_detector.entry_id                       1QZA 
_em_detector.id                             1 
_em_detector.type                           'KODAK SO163 FILM' 
_em_detector.detective_quantum_efficiency   ? 
_em_detector.details                        ? 
# 
_em_entity_assembly.id                              1 
_em_entity_assembly.assembly_id                     1 
_em_entity_assembly.type                            ? 
_em_entity_assembly.name                            tRNA 
_em_entity_assembly.go_id                           ? 
_em_entity_assembly.ipr_id                          ? 
_em_entity_assembly.details                         ? 
_em_entity_assembly.ebi_cell                        ? 
_em_entity_assembly.ebi_cellular_location           ? 
_em_entity_assembly.ebi_engineered                  ? 
_em_entity_assembly.ebi_expression_system           ? 
_em_entity_assembly.ebi_expression_system_plasmid   ? 
_em_entity_assembly.ebi_organelle                   ? 
_em_entity_assembly.ebi_organism_common             ? 
_em_entity_assembly.ebi_organism_scientific         ? 
_em_entity_assembly.ebi_strain                      ? 
_em_entity_assembly.ebi_tissue                      ? 
_em_entity_assembly.synonym                         ? 
# 
_em_image_scans.entry_id                1QZA 
_em_image_scans.id                      1 
_em_image_scans.number_digital_images   ? 
# 
_em_imaging.entry_id                        1QZA 
_em_imaging.id                              1 
_em_imaging.sample_support_id               1 
_em_imaging.detector_id                     1 
_em_imaging.scans_id                        1 
_em_imaging.date                            ? 
_em_imaging.temperature                     93 
_em_imaging.microscope_model                'FEI TECNAI F20' 
_em_imaging.nominal_defocus_min             2000 
_em_imaging.nominal_defocus_max             4000 
_em_imaging.tilt_angle_min                  0 
_em_imaging.tilt_angle_max                  0 
_em_imaging.nominal_cs                      ? 
_em_imaging.mode                            'BRIGHT FIELD' 
_em_imaging.electron_dose                   2000 
_em_imaging.illumination_mode               'FLOOD BEAM' 
_em_imaging.nominal_magnification           50000 
_em_imaging.calibrated_magnification        49696 
_em_imaging.electron_source                 'FIELD EMISSION GUN' 
_em_imaging.accelerating_voltage            200 
_em_imaging.details                         ? 
_em_imaging.specimen_holder_type            ? 
_em_imaging.specimen_holder_model           ? 
_em_imaging.citation_id                     ? 
_em_imaging.detector_distance               ? 
_em_imaging.energy_filter                   ? 
_em_imaging.energy_window                   ? 
_em_imaging.recording_temperature_maximum   ? 
_em_imaging.recording_temperature_minimum   ? 
_em_imaging.microscope_id                   1 
# 
_em_sample_support.id               1 
_em_sample_support.details          'Quantifoil holley carbon film grids' 
_em_sample_support.citation_id      ? 
_em_sample_support.film_material    ? 
_em_sample_support.grid_material    ? 
_em_sample_support.grid_mesh_size   ? 
_em_sample_support.grid_type        ? 
_em_sample_support.method           ? 
_em_sample_support.pretreatment     ? 
# 
_em_vitrification.entry_id                1QZA 
_em_vitrification.id                      1 
_em_vitrification.details                 'Rapid-freezing in liquid ethane' 
_em_vitrification.citation_id             ? 
_em_vitrification.cryogen_name            ? 
_em_vitrification.humidity                ? 
_em_vitrification.instrument              ? 
_em_vitrification.method                  ? 
_em_vitrification.sample_preparation_id   1 
_em_vitrification.temp                    ? 
_em_vitrification.time_resolved_state     ? 
# 
_pdbx_database_remark.id     999 
_pdbx_database_remark.text   
;SEQUENCE
THE STRUCTURE CONTAINS P ATOMS ONLY 
;
# 
loop_
_pdbx_version.entry_id 
_pdbx_version.revision_date 
_pdbx_version.major_version 
_pdbx_version.minor_version 
_pdbx_version.revision_type 
_pdbx_version.details 
1QZA 2008-04-29 3 2    'Version format compliance' 'compliance with PDB format V.3.15'          
1QZA 2011-07-13 4 0000 'Version format compliance' 'compliance with PDB Exchange Dictionary V4' 
# 
_pdbx_struct_assembly.id                   1 
_pdbx_struct_assembly.details              author_defined_assembly 
_pdbx_struct_assembly.method_details       ? 
_pdbx_struct_assembly.oligomeric_details   monomeric 
_pdbx_struct_assembly.oligomeric_count     1 
# 
_pdbx_struct_assembly_gen.assembly_id       1 
_pdbx_struct_assembly_gen.oper_expression   1 
_pdbx_struct_assembly_gen.asym_id_list      A 
# 
_pdbx_struct_oper_list.id                   1 
_pdbx_struct_oper_list.type                 'identity operation' 
_pdbx_struct_oper_list.name                 1_555 
_pdbx_struct_oper_list.symmetry_operation   x,y,z 
_pdbx_struct_oper_list.matrix[1][1]         1.0000000000 
_pdbx_struct_oper_list.matrix[1][2]         0.0000000000 
_pdbx_struct_oper_list.matrix[1][3]         0.0000000000 
_pdbx_struct_oper_list.vector[1]            0.0000000000 
_pdbx_struct_oper_list.matrix[2][1]         0.0000000000 
_pdbx_struct_oper_list.matrix[2][2]         1.0000000000 
_pdbx_struct_oper_list.matrix[2][3]         0.0000000000 
_pdbx_struct_oper_list.vector[2]            0.0000000000 
_pdbx_struct_oper_list.matrix[3][1]         0.0000000000 
_pdbx_struct_oper_list.matrix[3][2]         0.0000000000 
_pdbx_struct_oper_list.matrix[3][3]         1.0000000000 
_pdbx_struct_oper_list.vector[3]            0.0000000000 
# 
_em_sample_preparation.entry_id               1QZA 
_em_sample_preparation.id                     1 
_em_sample_preparation.buffer_id              ? 
_em_sample_preparation.support_id             ? 
_em_sample_preparation.sample_concentration   32 
_em_sample_preparation.ph                     7.5 
_em_sample_preparation.2d_crystal_grow_id     ? 
_em_sample_preparation.entity_assembly_id     . 
# 
_pdbx_coordinate_model.asym_id   A 
_pdbx_coordinate_model.type      'P ATOMS ONLY' 
# 
