;jsRoesy ;symmetrized CW-ROESY, program for DMX hardware, tested on DMX 500i and DMX750 ;of NMRFAM; AMX version run on AMX400 and AMX600 spectrometers ;by J. Schleucher, J. Quant, S.J. Glaser, C. Griesinger, University of Frankfurt 1993 ;js@nmrfam.wisc.edu ;BMRB Pulse Sequence Accession Number: 65 1 ze 2 d1 3 20u 20u pl9:f1 p18 ph29 ;presat 3u 20u pl1:f1 p1 ph1 d0 p1 ph2 ;"flanking pulse", see C. Griesinger & R.R. Ernst 2u ;JMR 75 (1987), 261-271 3u pl3:f1 ;switching power level for spin-lock 5 (p11:sp1 ph3):f1 ;shaped pulse for downfield spin-lock lo to 5 times l1 ;loop for downfield spin-lock 2u 3u pl2:f1 ;switching power level for 180 deg. pulses p6 ph4 ;1. 180 deg. pulse for realignment 3u p6 ph5 ;2. 180 deg. pulse for realignment 2u 3u pl3:f1 ;not absolutely necessary to switch back to tl0, 6 (p11:sp2 ph6):f1 ;since tp2 will be used anyway, highfield spin-lock lo to 6 times l2 ;loop for highfield spin-lock 2u 3u pl1:f1 ;switching back to high power for 2u p1 ph2 ;2. flanking pulse go=2 ph31 d1 wr #0 if #0 id0 ip1 zd lo to 3 times td1 exit ph1 = 0 2 2 0 1 3 3 1 ph2 = 0 0 2 2 1 1 3 3 ;ph3 = 0 0 2 2 1 1 3 3 ph3 = (360) 6 6 186 186 96 96 276 276 ph4 = 0 0 2 2 1 1 3 3 ;ph4 = (360) 160 160 340 340 250 250 70 70 ph5 = 2 2 0 0 3 3 1 1 ;ph5 = (360) 340 340 160 160 70 70 250 250 ;ph6 = 2 2 0 0 3 3 1 1 ph6 = (360) 186 186 6 6 276 276 96 96 ph29=0 ph31= 0 2 2 0 1 3 3 1 ;Comments: ;As standard parameters, I use a spin-lock field strength of gamma B1/(2*pi)= ;5 kHz (=tp1=tp2= 24 dB) and a lock angle of 63 deg (on resonance, measured ;from the z-axis). The off-resonant spin-lock is implemented using phase- ;modulated shaped pulses of constant amplitude. The shape I use consists of 720 ;real points, a phase increment of 5 deg. per step is applied (use the utilities- ;submenue of the program "shape" on AMX hardware to add a phase-gradient to a ;rectangular shaped pulse). This phase increment results in a total phase of 10 ;revolutions per shape. Therefore, the length of a shape is given by p11= ;(10*tan(63)/gamma B1). Alternatively, the phase- gradient can be generated ;using the parameter "tpoffset". In this case the length of p11 will have to be ;chosen such that the phase of p11 will make an integer number of revolutions, ;that is, p11 has to be a multiple of (1/offset frequency). The mixing time is ;adjusted using the loop counters l1, l2. l2 is chosen somewhat larger than l1. ;This prevents anwanted magnetisation to be refocused at the end of the second ;spin-lock. ;The field strength required for the 180 deg. pulses is given by ;2(gamma B1)/(sin(63 deg)^2)=12.6 kHz for a 5 kHz spin-lock. ;In the phase programs given above,the theoretical phases of the pulses are ;given as comments. Since the phase of the output of an amplifier is in general ;dependent on the amplitude of the output, the phases need to be corrected. The ;correction needed depends on the particular hardware, so you'll have to ;determine these phases once for the set of parameters you want to use. To ;determine the required phase correction of the low-power pulses, record a spectrum ;with a low-power pulse of the power chosen and apply the phase correction of a ;spectrum acquired using a hard pulse. The additional zero-order phase correction ;needed to phase the low-power spectrum to pure absorption on-resonance is the ;phase correction needed. Using AMX hardware, you can also use the parameter ;"phcor" instead of editing the phase-cycle. In the above program, the durations ;of delays used to switch power levels have been chosen minimal. The values proved ;sufficient on our hardware.