BMRB Entry 11519
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11519
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Title: The chemical shift assignments of the headpiece subdomain of chicken villin protein under the diluted condition
Deposition date: 2012-12-12 Original release date: 2013-02-15
Authors: Tochio, Naoya; Kigawa, Takanori
Citation: Harada, Ryuhei; Tochio, Naoya; Kigawa, Takanori; Sugita, Yuji; Feig, Michael. "Reduced native state stability in crowded cellular environment due to protein-protein interactions" Not known ., .-..
Assembly members:
protein, polymer, 36 residues, Formula weight is not available
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: E. coli - cell free
Entity Sequences (FASTA):
protein: MLSDEDFKAVFGMTRSAFAN
LPLWKQQNLKKEKGLF
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 34 |
15N chemical shifts | 33 |
1H chemical shifts | 33 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Villin | 1 |
Entities:
Entity 1, Villin 36 residues - Formula weight is not available
1 | MET | LEU | SER | ASP | GLU | ASP | PHE | LYS | ALA | VAL | ||||
2 | PHE | GLY | MET | THR | ARG | SER | ALA | PHE | ALA | ASN | ||||
3 | LEU | PRO | LEU | TRP | LYS | GLN | GLN | ASN | LEU | LYS | ||||
4 | LYS | GLU | LYS | GLY | LEU | PHE |
Samples:
sample_1: protein, [U-13C; U-15N], 1 mM; HEPES 50 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 0 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
Kujira, N. Kobayashi - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
BMRB | 11520 11521 15097 15245 17698 19911 4428 |
PDB | |
GB | AAA49133 |
REF | NP_990773 XP_003207697 XP_010711916 XP_010711917 |
SP | P02640 |
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SPARKY: Backbone
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