BMRB Entry 15930
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15930
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Title: Sequence-specific 1H, 15N, and 13C resonance assignments and relaxation parameters for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit in 10% TFE PubMed: 19847776
Deposition date: 2008-08-26 Original release date: 2012-01-04
Authors: Kaczka, Piotr; Polkowska, Agnieszka
Citation: Kaczka, Piotr; Polkowska, Agnieszka; Bolewska, Krystyna; Zhukov, Igor; Poznanski, Jaroslaw; Wierzchowski, Kazimierz. "Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit." Proteins 78, 754-768 (2010).
Assembly members:
4_sigma70, polymer, 107 residues, 12032.5 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
4_sigma70: MGSSHHHHHHSSGLVPRGSH
MLELPLDSATTESLRAATHD
VLAGLTAREAKVLRMRFGID
MNTDYTLEEVGKQFDVTRER
IRQIEAKALRKLRHPSRSEV
LRSFLDD
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 279 |
15N chemical shifts | 101 |
1H chemical shifts | 346 |
T1 relaxation values | 101 |
T2 relaxation values | 101 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | 4 sigma70 | 1 |
Entities:
Entity 1, 4 sigma70 107 residues - 12032.5 Da.
1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
3 | MET | LEU | GLU | LEU | PRO | LEU | ASP | SER | ALA | THR | ||||
4 | THR | GLU | SER | LEU | ARG | ALA | ALA | THR | HIS | ASP | ||||
5 | VAL | LEU | ALA | GLY | LEU | THR | ALA | ARG | GLU | ALA | ||||
6 | LYS | VAL | LEU | ARG | MET | ARG | PHE | GLY | ILE | ASP | ||||
7 | MET | ASN | THR | ASP | TYR | THR | LEU | GLU | GLU | VAL | ||||
8 | GLY | LYS | GLN | PHE | ASP | VAL | THR | ARG | GLU | ARG | ||||
9 | ILE | ARG | GLN | ILE | GLU | ALA | LYS | ALA | LEU | ARG | ||||
10 | LYS | LEU | ARG | HIS | PRO | SER | ARG | SER | GLU | VAL | ||||
11 | LEU | ARG | SER | PHE | LEU | ASP | ASP |
Samples:
sample_1: 4 sigma70, [U-13C; U-15N], 3 mM
sample_conditions_1: ionic strength: 0.0 M; pH: 4.55; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
{1H-} -15N NOE | sample_1 | isotropic | sample_conditions_1 |
{1H-} -15N T1 | sample_1 | isotropic | sample_conditions_1 |
{1H-} -15N T2 | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v1.7, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY v3.111, Goddard - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Varian UnityPlus 500 MHz
Related Database Links:
BMRB | 15936 15975 |
PDB | |
DBJ | BAB37373 BAE77118 BAG66772 BAG78873 BAH65247 |
EMBL | CAD07736 CAL34114 CAP77541 CAQ33404 CAQ90501 |
GB | AAA24601 AAA27242 AAA89147 AAB60181 AAC76103 |
PIR | AB0893 |
REF | NP_311977 NP_417539 NP_457602 NP_462126 NP_708877 |
SP | P00579 P0A2E3 P0A2E4 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts