BMRB Entry 16450
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16450
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Title: Backbone and side chain 1H, 13C, 15N chemical shift assignments for YSK2 PubMed: 19842064
Deposition date: 2009-08-17 Original release date: 2009-11-16
Authors: Findlater, Emma; Steffen, Graether
Citation: Findlater, Emma; Graether, Steffen. "NMR assignments of the intrinsically disordered K(2) and YSK (2) dehydrins" Biomol. NMR Assignments 3, 273-275 (2009).
Assembly members:
YSK2, polymer, 130 residues, Formula weight is not available
Natural source: Common Name: eudicots Taxonomy ID: 96939 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Vitis riparia
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
YSK2: MAYQQDPCANPTRQTGKTGG
QTDQYGNPVHQTEALGAYGA
GTGTGMHGGEHQQQPHQQPG
VLNRSGSSSSEDDGQGGRRK
KGMKEKIKERIPGMGRKDEQ
KQTSATSTPGQGQQQKGMME
KIKEKLPGAH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 398 |
15N chemical shifts | 109 |
1H chemical shifts | 615 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | YSK2 monomer | 1 |
Entities:
Entity 1, YSK2 monomer 130 residues - Formula weight is not available
1 | MET | ALA | TYR | GLN | GLN | ASP | PRO | CYS | ALA | ASN | |
2 | PRO | THR | ARG | GLN | THR | GLY | LYS | THR | GLY | GLY | |
3 | GLN | THR | ASP | GLN | TYR | GLY | ASN | PRO | VAL | HIS | |
4 | GLN | THR | GLU | ALA | LEU | GLY | ALA | TYR | GLY | ALA | |
5 | GLY | THR | GLY | THR | GLY | MET | HIS | GLY | GLY | GLU | |
6 | HIS | GLN | GLN | GLN | PRO | HIS | GLN | GLN | PRO | GLY | |
7 | VAL | LEU | ASN | ARG | SER | GLY | SER | SER | SER | SER | |
8 | GLU | ASP | ASP | GLY | GLN | GLY | GLY | ARG | ARG | LYS | |
9 | LYS | GLY | MET | LYS | GLU | LYS | ILE | LYS | GLU | ARG | |
10 | ILE | PRO | GLY | MET | GLY | ARG | LYS | ASP | GLU | GLN | |
11 | LYS | GLN | THR | SER | ALA | THR | SER | THR | PRO | GLY | |
12 | GLN | GLY | GLN | GLN | GLN | LYS | GLY | MET | MET | GLU | |
13 | LYS | ILE | LYS | GLU | LYS | LEU | PRO | GLY | ALA | HIS |
Samples:
sample_1: YSK2, [U-100% 13C; U-100% 15N], 0.6 mM; H2O 95%; D2O 5%; NaCl 10 mM
sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CcpNMR, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts