BMRB Entry 25339
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25339
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Title: Backbone amide assignments for human Cyclophilin C with saturating concentrations of the model peptide GSFGPDLRAGD PubMed: 25923019
Deposition date: 2014-11-13 Original release date: 2014-12-16
Authors: Holliday, Michael; Camilloni, Carlo; Armstrong, Geoffrey; Isern, Nancy; Zhang, Fengli; Vendruscolo, Michele; Eisenmesser, Elan
Citation: Holliday, Michael; Camilloni, Carlo; Armstrong, Geoffrey; Isern, Nancy; Zhang, Fengli; Vendruscolo, Michele; Eisenmesser, Elan. "Structure and Dynamics of GeoCyp: A Thermophilic Cyclophilin with a Novel Substrate Binding Mechanism That Functions Efficiently at Low Temperatures" Biochemistry 54, 3207-3217 (2015).
Assembly members:
CypC, polymer, 190 residues, 20515.3 Da.
GSFGPDLRAGD, polymer, 11 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CypC: GSGAEGFRKRGPSVTAKVFF
DVRIGDKDVGRIVIGLFGKV
VPKTVENFVALATGEKGYGY
KGSKFHRVIKDFMIQGGDIT
TGDGTGGVSIYGETFPDENF
KLKHYGIGWVSMANAGPDTN
GSQFFITLTKPTWLDGKHVV
FGKVIDGMTVVHSIELQATD
GHDRPLTNCSIINSGKIDVK
TPFVVEIADW
GSFGPDLRAGD: GSFGPDLRAGD
- assigned_chemical_shifts
- spectral_peak_list
Data type | Count |
15N chemical shifts | 175 |
1H chemical shifts | 175 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | CypC | 1 |
2 | GSFGPDLRAGD | 2 |
Entities:
Entity 1, CypC 190 residues - 20515.3 Da.
Residues 25-212 of human Cyclophilin C with two additional N-terminal residues, Gly-Ser-, remaining after removal of affinity tag
1 | GLY | SER | GLY | ALA | GLU | GLY | PHE | ARG | LYS | ARG | |
2 | GLY | PRO | SER | VAL | THR | ALA | LYS | VAL | PHE | PHE | |
3 | ASP | VAL | ARG | ILE | GLY | ASP | LYS | ASP | VAL | GLY | |
4 | ARG | ILE | VAL | ILE | GLY | LEU | PHE | GLY | LYS | VAL | |
5 | VAL | PRO | LYS | THR | VAL | GLU | ASN | PHE | VAL | ALA | |
6 | LEU | ALA | THR | GLY | GLU | LYS | GLY | TYR | GLY | TYR | |
7 | LYS | GLY | SER | LYS | PHE | HIS | ARG | VAL | ILE | LYS | |
8 | ASP | PHE | MET | ILE | GLN | GLY | GLY | ASP | ILE | THR | |
9 | THR | GLY | ASP | GLY | THR | GLY | GLY | VAL | SER | ILE | |
10 | TYR | GLY | GLU | THR | PHE | PRO | ASP | GLU | ASN | PHE | |
11 | LYS | LEU | LYS | HIS | TYR | GLY | ILE | GLY | TRP | VAL | |
12 | SER | MET | ALA | ASN | ALA | GLY | PRO | ASP | THR | ASN | |
13 | GLY | SER | GLN | PHE | PHE | ILE | THR | LEU | THR | LYS | |
14 | PRO | THR | TRP | LEU | ASP | GLY | LYS | HIS | VAL | VAL | |
15 | PHE | GLY | LYS | VAL | ILE | ASP | GLY | MET | THR | VAL | |
16 | VAL | HIS | SER | ILE | GLU | LEU | GLN | ALA | THR | ASP | |
17 | GLY | HIS | ASP | ARG | PRO | LEU | THR | ASN | CYS | SER | |
18 | ILE | ILE | ASN | SER | GLY | LYS | ILE | ASP | VAL | LYS | |
19 | THR | PRO | PHE | VAL | VAL | GLU | ILE | ALA | ASP | TRP |
Entity 2, GSFGPDLRAGD 11 residues - Formula weight is not available
1 | GLY | SER | PHE | GLY | PRO | ASP | LEU | ARG | ALA | GLY | ||||
2 | ASP |
Samples:
CypC-GSFGPDLRAGD: CypC, [U-13C; U-15N; U-2H], 1 mM; GSFGPDLRAGD 6 mM; sodium phosphate 50 mM; EDTA 1 mM; DTT 1 mM; H2O 93%; D2O 7%
sample_conditions: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | CypC-GSFGPDLRAGD | isotropic | sample_conditions |
3D 1H-15N NOESY | CypC-GSFGPDLRAGD | isotropic | sample_conditions |
Software:
CcpNmr_Analysis v2.4, CCPN - chemical shift assignment, peak picking
nmrPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Varian - Spectrum processing, collection
NMR spectrometers:
- Varian UnityInova 900 MHz
Related Database Links:
BMRB | 25341 |
PDB | |
DBJ | BAF83010 |
GB | AAB31350 AAH02678 ABO43038 ADQ32383 EAW48878 |
REF | NP_000934 NP_001180902 XP_002815869 XP_003310839 XP_003826742 |
SP | P45877 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts