BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10028

Title: Solution structure of the second WW domain from mouse salvador homolog 1 protein (mm45).   PubMed: 17239860

Deposition date: 2006-10-20 Original release date: 2007-10-19

Authors: Ohnishi, Satoshi; Kigawa, Takanori; Koshiba, Seizo; Tomizawa, Tadashi; Sato, Manami; Tochio, Naoya; Inoue, Makoto; Harada, Takushi; Watanabe, Satoru; Yokoyama, Shigeyuki

Citation: Ohnishi, S.; Guntert, P.; Koshiba, S.; Tomizawa, T.; Akasaka, R.; Tochio, N.; Sato, M.; Inoue, M.; Harada, T.; Watanabe, S.; Tanaka, A.; Shirouzu, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of an atypical WW domain in a novel beta-clam-like dimeric form"  Febs Lett. 581, 462-468 (2007).

Assembly members:
WW domain, polymer, 49 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
WW domain: GSSGSSGPLEREGLPPGWER VESSEFGTYYVDHTNKRAQY RHPSGPSSG

Data sets:
Data typeCount
13C chemical shifts184
15N chemical shifts39
1H chemical shifts269

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1WW domain 11
2WW domain 21

Entities:

Entity 1, WW domain 1 49 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROLEUGLU
2   ARGGLUGLYLEUPROPROGLYTRPGLUARG
3   VALGLUSERSERGLUPHEGLYTHRTYRTYR
4   VALASPHISTHRASNLYSARGALAGLNTYR
5   ARGHISPROSERGLYPROSERSERGLY

Samples:

sample_1: WW domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 0.12 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.935, Kobayashi, N. - data analysis

CYANA v2.2, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download simulated HSQC data in one of the following formats:
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