BMRB Entry 10057

Name: Solution structure of Iron-sulfur cluster protein U (IscU)
Published: 2008-08-13

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PDB ID: 1wfz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10057
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of Iron-sulfur cluster protein U (IscU)

Deposition date: 2006-11-29 Original release date: 2008-08-13

Authors: Nakanishi, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Nakanishi, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of Iron-sulfur cluster protein U (IscU)" . ., .-..

Assembly members:
Iron-sulfur cluster protein U (IscU), polymer, 130 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: cell free synthesis

Entity Sequences (FASTA):
Iron-sulfur cluster protein U (IscU): GSSGSSGENPRNVGSLDKTS KNVGTGLVGAPACGDVMKLQ IQVDEKGKIVDARFKTFGCG SAIASSSLATEWVKGKTVEE ALTIKNTDIAKELCLPPVKL HCSMLAEDAIKAALADYKLK QESKSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	508
15N chemical shifts	124
1H chemical shifts	852

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	nitrogen fixation cluster-like	1
2	ZINC (II) ION	2

1	GLY	SER	SER	GLY	SER	SER	GLY	GLU	ASN	PRO
2	ARG	ASN	VAL	GLY	SER	LEU	ASP	LYS	THR	SER
3	LYS	ASN	VAL	GLY	THR	GLY	LEU	VAL	GLY	ALA
4	PRO	ALA	CYS	GLY	ASP	VAL	MET	LYS	LEU	GLN
5	ILE	GLN	VAL	ASP	GLU	LYS	GLY	LYS	ILE	VAL
6	ASP	ALA	ARG	PHE	LYS	THR	PHE	GLY	CYS	GLY
7	SER	ALA	ILE	ALA	SER	SER	SER	LEU	ALA	THR
8	GLU	TRP	VAL	LYS	GLY	LYS	THR	VAL	GLU	GLU
9	ALA	LEU	THR	ILE	LYS	ASN	THR	ASP	ILE	ALA
10	LYS	GLU	LEU	CYS	LEU	PRO	PRO	VAL	LYS	LEU
11	HIS	CYS	SER	MET	LEU	ALA	GLU	ASP	ALA	ILE
12	LYS	ALA	ALA	LEU	ALA	ASP	TYR	LYS	LEU	LYS
13	GLN	GLU	SER	LYS	SER	GLY	PRO	SER	SER	GLY

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	not available	condition_1
3D 13C-separated NOESY	sample_1	not available	condition_1

PDB	1WFZ
DBJ	BAB26031 BAC30464 BAC38830 BAE91311 BAG51892
EMBL	CAL38012
GB	AAC50885 AAG37427 AAG37428 AAH11906 AAH28800
REF	NP_001069151 NP_001099406 NP_001230245 NP_001248403 NP_001270426
SP	Q9D7P6 Q9H1K1
TPG	DAA20564

Biological Magnetic Resonance Data Bank

BMRB Entry 10057

Title: Solution structure of Iron-sulfur cluster protein U (IscU)

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	SER	GLY	SER	SER	GLY	GLU	ASN	PRO
2	ARG	ASN	VAL	GLY	SER	LEU	ASP	LYS	THR	SER
3	LYS	ASN	VAL	GLY	THR	GLY	LEU	VAL	GLY	ALA
4	PRO	ALA	CYS	GLY	ASP	VAL	MET	LYS	LEU	GLN
5	ILE	GLN	VAL	ASP	GLU	LYS	GLY	LYS	ILE	VAL
6	ASP	ALA	ARG	PHE	LYS	THR	PHE	GLY	CYS	GLY
7	SER	ALA	ILE	ALA	SER	SER	SER	LEU	ALA	THR
8	GLU	TRP	VAL	LYS	GLY	LYS	THR	VAL	GLU	GLU
9	ALA	LEU	THR	ILE	LYS	ASN	THR	ASP	ILE	ALA
10	LYS	GLU	LEU	CYS	LEU	PRO	PRO	VAL	LYS	LEU
11	HIS	CYS	SER	MET	LEU	ALA	GLU	ASP	ALA	ILE
12	LYS	ALA	ALA	LEU	ALA	ASP	TYR	LYS	LEU	LYS
13	GLN	GLU	SER	LYS	SER	GLY	PRO	SER	SER	GLY

1	GLY	SER	SER	GLY	SER	SER	GLY	GLU	ASN	PRO
2	ARG	ASN	VAL	GLY	SER	LEU	ASP	LYS	THR	SER
3	LYS	ASN	VAL	GLY	THR	GLY	LEU	VAL	GLY	ALA
4	PRO	ALA	CYS	GLY	ASP	VAL	MET	LYS	LEU	GLN
5	ILE	GLN	VAL	ASP	GLU	LYS	GLY	LYS	ILE	VAL
6	ASP	ALA	ARG	PHE	LYS	THR	PHE	GLY	CYS	GLY
7	SER	ALA	ILE	ALA	SER	SER	SER	LEU	ALA	THR
8	GLU	TRP	VAL	LYS	GLY	LYS	THR	VAL	GLU	GLU
9	ALA	LEU	THR	ILE	LYS	ASN	THR	ASP	ILE	ALA
10	LYS	GLU	LEU	CYS	LEU	PRO	PRO	VAL	LYS	LEU
11	HIS	CYS	SER	MET	LEU	ALA	GLU	ASP	ALA	ILE
12	LYS	ALA	ALA	LEU	ALA	ASP	TYR	LYS	LEU	LYS
13	GLN	GLU	SER	LYS	SER	GLY	PRO	SER	SER	GLY

1	GLY	SER	SER	GLY	SER	SER	GLY	GLU	ASN	PRO
2	ARG	ASN	VAL	GLY	SER	LEU	ASP	LYS	THR	SER
3	LYS	ASN	VAL	GLY	THR	GLY	LEU	VAL	GLY	ALA
4	PRO	ALA	CYS	GLY	ASP	VAL	MET	LYS	LEU	GLN
5	ILE	GLN	VAL	ASP	GLU	LYS	GLY	LYS	ILE	VAL
6	ASP	ALA	ARG	PHE	LYS	THR	PHE	GLY	CYS	GLY
7	SER	ALA	ILE	ALA	SER	SER	SER	LEU	ALA	THR
8	GLU	TRP	VAL	LYS	GLY	LYS	THR	VAL	GLU	GLU
9	ALA	LEU	THR	ILE	LYS	ASN	THR	ASP	ILE	ALA
10	LYS	GLU	LEU	CYS	LEU	PRO	PRO	VAL	LYS	LEU
11	HIS	CYS	SER	MET	LEU	ALA	GLU	ASP	ALA	ILE
12	LYS	ALA	ALA	LEU	ALA	ASP	TYR	LYS	LEU	LYS
13	GLN	GLU	SER	LYS	SER	GLY	PRO	SER	SER	GLY