BMRB Entry 10079
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10079
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of putative domain of human KIAA0561 protein
Deposition date: 2007-01-23 Original release date: 2008-08-14
Authors: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of putative domain of human KIAA0561 protein" . ., .-..
Assembly members:
putative domain (residues 180-280), polymer, 114 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
putative domain (residues 180-280): GSSGSSGPKATAQMEGRLQE
FLTAYAPGARLALADGVLGF
IHHQIVELARDCLAKSGENL
VTSRYFLEMQEKLERLLQDA
HERSDSEEVSFIVQLVRKLL
IIISRPARSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 453 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 716 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KIAA0561 protein | 1 |
Entities:
Entity 1, KIAA0561 protein 114 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | LYS | ALA | ||||
| 2 | THR | ALA | GLN | MET | GLU | GLY | ARG | LEU | GLN | GLU | ||||
| 3 | PHE | LEU | THR | ALA | TYR | ALA | PRO | GLY | ALA | ARG | ||||
| 4 | LEU | ALA | LEU | ALA | ASP | GLY | VAL | LEU | GLY | PHE | ||||
| 5 | ILE | HIS | HIS | GLN | ILE | VAL | GLU | LEU | ALA | ARG | ||||
| 6 | ASP | CYS | LEU | ALA | LYS | SER | GLY | GLU | ASN | LEU | ||||
| 7 | VAL | THR | SER | ARG | TYR | PHE | LEU | GLU | MET | GLN | ||||
| 8 | GLU | LYS | LEU | GLU | ARG | LEU | LEU | GLN | ASP | ALA | ||||
| 9 | HIS | GLU | ARG | SER | ASP | SER | GLU | GLU | VAL | SER | ||||
| 10 | PHE | ILE | VAL | GLN | LEU | VAL | ARG | LYS | LEU | LEU | ||||
| 11 | ILE | ILE | ILE | SER | ARG | PRO | ALA | ARG | SER | GLY | ||||
| 12 | PRO | SER | SER | GLY |
Samples:
sample_1: putative domain, [U-13C; U-15N], 1.3 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.853, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA25487 BAI45348 |
| GB | AAC62830 EAW84657 EHH29807 EHH59364 |
| REF | NP_055831 XP_001115216 XP_003915213 XP_004060339 XP_005259879 |
| SP | O60307 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts