BMRB Entry 10091
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10091
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Title: Solution structure of the C-terminal ubiquitin-like domain of mouse tubulin-specific chaperone e
Deposition date: 2007-01-24 Original release date: 2008-08-14
Authors: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C-terminal ubiquitin-like domain of mouse tubulin-specific chaperone e" . ., .-..
Assembly members:
ubiquitin like-domain, polymer, 97 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
ubiquitin like-domain: GSSGSSGQLLTLKIKCSNQP
ERQILEKQLPDSMTVQKVKG
LLSRLLKVPVSELLLSYESS
KMPGREIELENDLQPLQFYS
VENGDCLLVRWSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 422 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 689 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tubulin-folding protein TBCE | 1 |
Entities:
Entity 1, Tubulin-folding protein TBCE 97 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLN | LEU | LEU | ||||
| 2 | THR | LEU | LYS | ILE | LYS | CYS | SER | ASN | GLN | PRO | ||||
| 3 | GLU | ARG | GLN | ILE | LEU | GLU | LYS | GLN | LEU | PRO | ||||
| 4 | ASP | SER | MET | THR | VAL | GLN | LYS | VAL | LYS | GLY | ||||
| 5 | LEU | LEU | SER | ARG | LEU | LEU | LYS | VAL | PRO | VAL | ||||
| 6 | SER | GLU | LEU | LEU | LEU | SER | TYR | GLU | SER | SER | ||||
| 7 | LYS | MET | PRO | GLY | ARG | GLU | ILE | GLU | LEU | GLU | ||||
| 8 | ASN | ASP | LEU | GLN | PRO | LEU | GLN | PHE | TYR | SER | ||||
| 9 | VAL | GLU | ASN | GLY | ASP | CYS | LEU | LEU | VAL | ARG | ||||
| 10 | TRP | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: ubiquitin-like domain, [U-13C; U-15N], 1.1 mM; d-Tris-HCl 20 mM; NaCl 200 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.8994, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAE39475 |
| GB | AAH50206 AAL92570 EDL32750 EDL32752 EDL32753 |
| REF | NP_848027 XP_006516836 XP_006516838 |
| SP | Q8CIV8 |
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