BMRB Entry 10289
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10289
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Title: Solution structure of the homeobox domain of Zinc finger homeobox protein 1b (Smad interacting protein 1)
Deposition date: 2008-12-17 Original release date: 2009-12-17
Authors: Ohnishi, S.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Ohnishi, S.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the homeobox domain of Zinc finger homeobox protein 1b (Smad interacting protein 1)" . ., .-..
Assembly members:
homeobox domain, polymer, 71 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
homeobox domain: GSSGSSGSPINPYKDHMSVL
KAYYAMNMEPNSDELLKISI
AVGLPQEFVKEWFEQRKVYQ
YSNSRSGPSSG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 292 |
15N chemical shifts | 63 |
1H chemical shifts | 443 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | homeobox domain | 1 |
Entities:
Entity 1, homeobox domain 71 residues - Formula weight is not available
1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | PRO | ILE | ||||
2 | ASN | PRO | TYR | LYS | ASP | HIS | MET | SER | VAL | LEU | ||||
3 | LYS | ALA | TYR | TYR | ALA | MET | ASN | MET | GLU | PRO | ||||
4 | ASN | SER | ASP | GLU | LEU | LEU | LYS | ILE | SER | ILE | ||||
5 | ALA | VAL | GLY | LEU | PRO | GLN | GLU | PHE | VAL | LYS | ||||
6 | GLU | TRP | PHE | GLU | GLN | ARG | LYS | VAL | TYR | GLN | ||||
7 | TYR | SER | ASN | SER | ARG | SER | GLY | PRO | SER | SER | ||||
8 | GLY |
Samples:
sample_1: homeobox domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
PDB | |
DBJ | BAA25495 BAB40819 BAC65594 BAE24572 BAG72873 |
EMBL | CAH90122 CAJ29798 |
GB | AAD56590 AAH60699 AAI27102 AAI27103 AAK52081 |
REF | NP_001028873 NP_001125020 NP_001165124 NP_001276450 NP_055610 |
SP | O60315 Q9R0G7 |
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