BMRB Entry 11010
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PDB ID: 2rmk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR11010
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Title: Rac1/PRK1 Complex PubMed: 18006505
Deposition date: 2007-10-25 Original release date: 2008-06-27
Authors: Modha, Rakhee; Campbell, Louise; Nietlispach, Daniel; Buhecha, Heeran; Owen, Darerca; Mott, Helen
Citation: Modha, Rakhee; Campbell, Louise; Nietlispach, Daniel; Buhecha, Heeran; Owen, Darerca; Mott, Helen. "The Rac1 polybasic region is required for interaction with its effector PRK1" J. Biol. Chem. 283, 1492-1500 (2008).
Assembly members:
Rac1, polymer, 192 residues, 21477.398 Da.
PRK1_HR1b, polymer, 81 residues, 9019.512 Da.
GCP, non-polymer, 521.208 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Rac1: MQAIKCVVVGDGAVGKTCLL
ISYTTNAFPGEYIPTVFDNY
SANVMVDGKPVNLGLWDTAG
LEDYDRLRPLSYPQTDVFLI
CFSLVSPASFENVRAKWYPE
VRHHCPNTPIILVGTKLDLR
DDKDTIEKLKEKKLTPITYP
QGLAMAKEIGAVKYLECSAL
TQRGLKTVFDEAIRAVLCPP
PVKKRKRKCLLL
PRK1_HR1b: GIPATNLSRVAGLEKQLAIE
LKVKQGAENMIQTYSNGSTK
DRKLLLTAQQMLQDSKTKID
IIRMQLRRALQADQLENQAA
P
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 970 |
| 15N chemical shifts | 248 |
| 1H chemical shifts | 1666 |
Additional metadata:
Related Database Links:
| SP | P63000 P62998 P62999 P63000 P63001 Q6RUV5 |
| PDB | 1E96 1FOE 1G4U 1HE1 1HH4 1I4D 1I4L 1I4T 1MH1 2FJU 2H7V 2NZ8 2P2L 2RMK 2VRW 2WKP 2WKQ 2WKR 2YIN 3B13 3BJI 3RYT 3SBD 3SBE 3SU8 3SUA 3TH5 4GZL 4GZM 4YON |
| DBJ | BAB25667 BAB26027 BAB69451 BAC16311 BAC28767 |
| EMBL | CAA39801 CAA40545 CAB53579 CAJ83626 CDQ72552 |
| GB | AAA36537 AAA36544 AAB22206 AAC18960 AAD50299 |
| REF | NP_001003274 NP_001004840 NP_001034907 NP_001084224 NP_001089332 |
| TPG | DAA15019 |
| SWS | Q16512 |
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