BMRB Entry 11070
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11070
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Title: DPC micelle bound structure of 1-28 GBP (growth-blocking peptide) PubMed: 19710009
Deposition date: 2009-04-07 Original release date: 2009-09-04
Authors: Umetsu, Yoshitaka; Aizawa, Tomoyasu; Muto, Kaori; Yamamoto, Hiroko; Kamiya, Masakatsu; Kumaki, Yasuhiro; Mizuguchi, Mineyuki; Demura, Makoto; Hayakawa, Yoichi; Kawano, Keiichi
Citation: Umetsu, Yoshitaka; Aizawa, Tomoyasu; Muto, Kaori; Yamamoto, Hiroko; Kamiya, Masakatsu; Kumaki, Yasuhiro; Mizuguchi, Mineyuki; Demura, Makoto; Hayakawa, Yoichi; Kawano, Keiichi. "C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity." J. Biol. Chem. 284, 29625-29634 (2009).
Assembly members:
1-28 GBP, polymer, 28 residues, Formula weight is not available
Natural source: Common Name: armyworm Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Spodoptera not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
1-28 GBP: ENFSGGCVAGYMRTPDGRCK
PTFYQLIT
- assigned_chemical_shifts
Data type | Count |
15N chemical shifts | 24 |
1H chemical shifts | 175 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | 1-28 GBP | 1 |
Entities:
Entity 1, 1-28 GBP 28 residues - Formula weight is not available
1 | GLU | ASN | PHE | SER | GLY | GLY | CYS | VAL | ALA | GLY | ||||
2 | TYR | MET | ARG | THR | PRO | ASP | GLY | ARG | CYS | LYS | ||||
3 | PRO | THR | PHE | TYR | GLN | LEU | ILE | THR |
Samples:
sample_1: 1-28 GBP 3 mM; DPC, [U-99% 2H], 200 mM; DSS 0.1 mM; H2O 90%; D2O 10%
sample_2: 1-28 GBP, [U-99% 15N], 1 mM; DPC, [U-99% 2H], 100 mM; DSS 0.1 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 4.4; pressure: 1 atm; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_2 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- JEOL ECA 600 MHz
- Bruker DRX 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts