BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11117

Title: The solution structure of the second fibronectin type III domain of mouse Ephrin type-A receptor 1

Deposition date: 2010-03-31 Original release date: 2011-04-01

Authors: Tochio, N.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Tochio, N.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the second fibronectin type III domain of mouse Ephrin type-A receptor 1"  . ., .-..

Assembly members:
Fibronectin type-III domain, polymer, 107 residues, Formula weight is not available

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
Fibronectin type-III domain: GSSGSSGAESLSGLSLKLVK KEPRQLELTWAGSRPRNPGG NLSYELHVLNQDEEWHQMVL EPRVLLTKLQPDTTYIVRVR TLTPLGPGPFSPDHEFRTSP PSGPSSG

Data sets:
Data typeCount
13C chemical shifts445
15N chemical shifts95
1H chemical shifts692

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fibronectin type-III domain1

Entities:

Entity 1, Fibronectin type-III domain 107 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYALAGLUSER
2   LEUSERGLYLEUSERLEULYSLEUVALLYS
3   LYSGLUPROARGGLNLEUGLULEUTHRTRP
4   ALAGLYSERARGPROARGASNPROGLYGLY
5   ASNLEUSERTYRGLULEUHISVALLEUASN
6   GLNASPGLUGLUTRPHISGLNMETVALLEU
7   GLUPROARGVALLEULEUTHRLYSLEUGLN
8   PROASPTHRTHRTYRILEVALARGVALARG
9   THRLEUTHRPROLEUGLYPROGLYPROPHE
10   SERPROASPHISGLUPHEARGTHRSERPRO
11   PROSERGLYPROSERSERGLY

Samples:

sample_1: FN3 domain, [U-13C; U-15N], 1.2 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAC25971
GB AAC52384 AAG12206 AAH71215 EDL13481 EDM15513
PRF 2207387A
REF NP_001101328 NP_076069 XP_003504401 XP_005363880 XP_005405417
SP Q60750

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts