BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11157

Title: Solution structure of the BTK motif of human Cytoplasmic tyrosine-protein kinase BMX

Deposition date: 2010-04-15 Original release date: 2011-05-05

Authors: Abe, H.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Yoneyama, M.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Abe, H.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Yoneyama, M.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the BTK motif of human Cytoplasmic tyrosine-protein kinase BMX"  . ., .-..

Assembly members:
BTK, polymer, 50 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
BTK: GSSGSSGNPHLLVKYHSGFF VDGKFLCCQQSCKAAPGCTL WEAYSGPSSG

Data sets:
Data typeCount
13C chemical shifts176
15N chemical shifts39
1H chemical shifts264

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BTK1
2ZINC ION2

Entities:

Entity 1, BTK 50 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNPROHIS
2   LEULEUVALLYSTYRHISSERGLYPHEPHE
3   VALASPGLYLYSPHELEUCYSCYSGLNGLN
4   SERCYSLYSALAALAPROGLYCYSTHRLEU
5   TRPGLUALATYRSERGLYPROSERSERGLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: BTK, [U-13C; U-15N], 1.29 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAG36341
GB AAC08966
REF XP_002831455 XP_003261130 XP_003805761 XP_012356387 XP_012640214

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts