BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11197

Title: Solution structure of the sixth PDZ domain of human InaD-like protein

Deposition date: 2010-07-21 Original release date: 2011-07-20

Authors: Inoue, K.; Hayashi, F.; Yokoyama, S.

Citation: Inoue, K.; Hayashi, F.; Yokoyama, S.. "Solution structure of the sixth PDZ domain of human InaD-like protein"  . ., .-..

Assembly members:
PDZ domain, polymer, 117 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
PDZ domain: GSSGSSGPNFSHWGPPRIVE IFREPNVSLGISIVGGQTVI KRLKNGEELKGIFIKQVLED SPAGKTNALKTGDKILEVSG VDLQNASHSEAVEAIKNAGN PVVFIVQSLSSTPRVIP

Data sets:
Data typeCount
13C chemical shifts481
15N chemical shifts111
1H chemical shifts792

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain1

Entities:

Entity 1, PDZ domain 117 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROASNPHE
2   SERHISTRPGLYPROPROARGILEVALGLU
3   ILEPHEARGGLUPROASNVALSERLEUGLY
4   ILESERILEVALGLYGLYGLNTHRVALILE
5   LYSARGLEULYSASNGLYGLUGLULEULYS
6   GLYILEPHEILELYSGLNVALLEUGLUASP
7   SERPROALAGLYLYSTHRASNALALEULYS
8   THRGLYASPLYSILELEUGLUVALSERGLY
9   VALASPLEUGLNASNALASERHISSERGLU
10   ALAVALGLUALAILELYSASNALAGLYASN
11   PROVALVALPHEILEVALGLNSERLEUSER
12   SERTHRPROARGVALILEPRO

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.19 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20060324, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9807, Kobayashi, N. - data analysis

CYANA v2.1, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB EHH61906

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts