BMRB Entry 11221
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11221
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Title: Solution structure of the Ig-like domain (615-713) from human Obscurin-like protein 1
Deposition date: 2010-07-22 Original release date: 2011-07-21
Authors: Qin, X.; Suetake, T.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Suetake, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the Ig-like domain (615-713) from human Obscurin-like protein 1" . ., .-..
Assembly members:
Ig-like domain, polymer, 112 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Ig-like domain: GSSGSSGTARLVAGLEDVQV
YDGEDAVFSLDLSTIIQGTW
FLNGEELKSNEPEGQVEPGA
LRYRIEQKGLQHRLILHAVK
HQDSGALVGFSCPGVQDSAA
LTIQESSGPSSG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 452 |
15N chemical shifts | 112 |
1H chemical shifts | 721 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Ig-like domain of Obscurin-like protein 1 | 1 |
Entities:
Entity 1, Ig-like domain of Obscurin-like protein 1 112 residues - Formula weight is not available
1 | GLY | SER | SER | GLY | SER | SER | GLY | THR | ALA | ARG | ||||
2 | LEU | VAL | ALA | GLY | LEU | GLU | ASP | VAL | GLN | VAL | ||||
3 | TYR | ASP | GLY | GLU | ASP | ALA | VAL | PHE | SER | LEU | ||||
4 | ASP | LEU | SER | THR | ILE | ILE | GLN | GLY | THR | TRP | ||||
5 | PHE | LEU | ASN | GLY | GLU | GLU | LEU | LYS | SER | ASN | ||||
6 | GLU | PRO | GLU | GLY | GLN | VAL | GLU | PRO | GLY | ALA | ||||
7 | LEU | ARG | TYR | ARG | ILE | GLU | GLN | LYS | GLY | LEU | ||||
8 | GLN | HIS | ARG | LEU | ILE | LEU | HIS | ALA | VAL | LYS | ||||
9 | HIS | GLN | ASP | SER | GLY | ALA | LEU | VAL | GLY | PHE | ||||
10 | SER | CYS | PRO | GLY | VAL | GLN | ASP | SER | ALA | ALA | ||||
11 | LEU | THR | ILE | GLN | GLU | SER | SER | GLY | PRO | SER | ||||
12 | SER | GLY |
Samples:
sample_1: Ig-like domain, [U-13C; U-15N], 1.10 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, varian - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9816, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
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