BMRB Entry 11233
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11233
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Title: Solution structure of the SH3 domain of human KIAA1783 protein
Deposition date: 2010-07-23 Original release date: 2011-08-03
Authors: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the SH3 domain of human KIAA1783 protein" . ., .-..
Assembly members:
SH3 domain, polymer, 85 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
SH3 domain: GSSGSSGSGYVIALRSYITD
NCSLLSFHRGDLIKLLPVAT
LEPGWQFGSAGGRSGLFPAD
IVQPAAAPDFSFSKEQRSGS
GPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 338 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 514 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 85 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | GLY | TYR | ||||
| 2 | VAL | ILE | ALA | LEU | ARG | SER | TYR | ILE | THR | ASP | ||||
| 3 | ASN | CYS | SER | LEU | LEU | SER | PHE | HIS | ARG | GLY | ||||
| 4 | ASP | LEU | ILE | LYS | LEU | LEU | PRO | VAL | ALA | THR | ||||
| 5 | LEU | GLU | PRO | GLY | TRP | GLN | PHE | GLY | SER | ALA | ||||
| 6 | GLY | GLY | ARG | SER | GLY | LEU | PHE | PRO | ALA | ASP | ||||
| 7 | ILE | VAL | GLN | PRO | ALA | ALA | ALA | PRO | ASP | PHE | ||||
| 8 | SER | PHE | SER | LYS | GLU | GLN | ARG | SER | GLY | SER | ||||
| 9 | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.15 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
Delta v4.3, JEOL - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A - data analysis
Kujira v0.9296, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- JEOL ECA 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAB47412 BAG10147 |
| GB | EAW89288 |
| REF | NP_001296171 XP_001095714 XP_002827884 XP_004041092 XP_009431703 |
| SP | Q96JP2 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts