BMRB Entry 11237
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11237
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Title: Solution structure of the first and second PHD domain from Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog
Deposition date: 2010-07-23 Original release date: 2011-08-03
Authors: Qin, X.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first and second PHD domain from Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog" . ., .-..
Assembly members:
PHD domain, polymer, 111 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
PHD domain: GSSGSSGANCAVCDSPGDLL
DQFFCTTCGQHYHGMCLDIA
VTPLKRAGWQCPECKVCQNC
KQSGEDSKMLVCDTCDKGYH
TFCLQPVMKSVPTNGWKCKN
CRICISGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 454 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 699 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PHD domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
| 4 | ZINC ION no.3 | 2 |
| 5 | ZINC ION no.4 | 2 |
Entities:
Entity 1, PHD domain 111 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ALA | ASN | CYS | ||||
| 2 | ALA | VAL | CYS | ASP | SER | PRO | GLY | ASP | LEU | LEU | ||||
| 3 | ASP | GLN | PHE | PHE | CYS | THR | THR | CYS | GLY | GLN | ||||
| 4 | HIS | TYR | HIS | GLY | MET | CYS | LEU | ASP | ILE | ALA | ||||
| 5 | VAL | THR | PRO | LEU | LYS | ARG | ALA | GLY | TRP | GLN | ||||
| 6 | CYS | PRO | GLU | CYS | LYS | VAL | CYS | GLN | ASN | CYS | ||||
| 7 | LYS | GLN | SER | GLY | GLU | ASP | SER | LYS | MET | LEU | ||||
| 8 | VAL | CYS | ASP | THR | CYS | ASP | LYS | GLY | TYR | HIS | ||||
| 9 | THR | PHE | CYS | LEU | GLN | PRO | VAL | MET | LYS | SER | ||||
| 10 | VAL | PRO | THR | ASN | GLY | TRP | LYS | CYS | LYS | ASN | ||||
| 11 | CYS | ARG | ILE | CYS | ILE | SER | GLY | PRO | SER | SER | ||||
| 12 | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: PHD domain, [U-13C; U-15N], 0.94 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 900 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts