BMRB Entry 11256
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11256
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Title: Solution Structure of the N-terminal Ubiquitin-like Domain of Mouse Ubiquitin Specific Protease 14 (USP14)
Deposition date: 2010-08-09 Original release date: 2011-08-17
Authors: Zhao, C.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Zhao, C.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the N-terminal Ubiquitin-like Domain of Mouse Ubiquitin Specific Protease 14 (USP14)" . ., .-..
Assembly members:
ubiquitin-like domain, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
ubiquitin-like domain: GSSGSSGYSVTVKWGKEKFE
GVELNTDEPPMVFKAQLFAL
TGVQPARQKVMVKGGTLKDD
DWGNIKMKNGMTVLMMGSAD
ALPEEPSAKTSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 408 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 634 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ubiquitin-like domain | 1 |
Entities:
Entity 1, ubiquitin-like domain 96 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | TYR | SER | VAL | ||||
| 2 | THR | VAL | LYS | TRP | GLY | LYS | GLU | LYS | PHE | GLU | ||||
| 3 | GLY | VAL | GLU | LEU | ASN | THR | ASP | GLU | PRO | PRO | ||||
| 4 | MET | VAL | PHE | LYS | ALA | GLN | LEU | PHE | ALA | LEU | ||||
| 5 | THR | GLY | VAL | GLN | PRO | ALA | ARG | GLN | LYS | VAL | ||||
| 6 | MET | VAL | LYS | GLY | GLY | THR | LEU | LYS | ASP | ASP | ||||
| 7 | ASP | TRP | GLY | ASN | ILE | LYS | MET | LYS | ASN | GLY | ||||
| 8 | MET | THR | VAL | LEU | MET | MET | GLY | SER | ALA | ASP | ||||
| 9 | ALA | LEU | PRO | GLU | GLU | PRO | SER | ALA | LYS | THR | ||||
| 10 | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: ubiquitin-like domain mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.8996, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA93551 BAB27544 BAC26713 BAC32528 BAE89403 |
| GB | AAB60365 AAH03556 AAH05571 AAH50197 AAH85947 |
| REF | NP_001008302 NP_001032411 NP_001033678 NP_001038961 NP_001068657 |
| SP | P54578 P60051 Q0IIF7 Q9JMA1 |
| TPG | DAA15782 |
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