BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11282

Title: Solution Structure of the UBA domain in Human Protein FAM100B

Deposition date: 2010-08-09 Original release date: 2011-08-18

Authors: Zhao, C.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation: Zhao, C.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the UBA domain in Human Protein FAM100B"  . ., .-..

Assembly members:
UBA-like domain, residues 8-66, polymer, 66 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
UBA-like domain, residues 8-66: GSSGSSGMSVNMDELRHQVM INQFVLAAGCAADQAKQLLQ AAHWQFETALSTFFQETNIP NSHHHH

Data sets:
Data typeCount
13C chemical shifts234
15N chemical shifts63
1H chemical shifts373

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UBA-like domain, residues 8-661

Entities:

Entity 1, UBA-like domain, residues 8-66 66 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETSERVAL
2   ASNMETASPGLULEUARGHISGLNVALMET
3   ILEASNGLNPHEVALLEUALAALAGLYCYS
4   ALAALAASPGLNALALYSGLNLEULEUGLN
5   ALAALAHISTRPGLNPHEGLUTHRALALEU
6   SERTHRPHEPHEGLNGLUTHRASNILEPRO
7   ASNSERHISHISHISHIS

Samples:

sample_1: UBA-like domain, [U-13C; U-15N], 0.43 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9748, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
GB AAH35511 AAH60859 ABW03765 EAW89383 EHA99413
REF NP_872371 XP_001151089 XP_002696214 XP_003340352 XP_003358005
SP Q8IYN6
TPG DAA18129

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts