BMRB Entry 11297
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11297
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Title: Solusion structure of the Todor domain of human Lamin-B receptor
Deposition date: 2010-08-09 Original release date: 2011-08-19
Authors: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solusion structure of the Todor domain of human Lamin-B receptor" . ., .-..
Assembly members:
Tudor domain, polymer, 68 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Tudor domain: GSSGSSGMPSRKFADGEVVR
GRWPGSSLYYEVEILSHDST
SQLYTVKYKDGTELELKEND
IKSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 258 |
| 15N chemical shifts | 59 |
| 1H chemical shifts | 408 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tudor domain | 1 |
Entities:
Entity 1, Tudor domain 68 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | PRO | SER | ||||
| 2 | ARG | LYS | PHE | ALA | ASP | GLY | GLU | VAL | VAL | ARG | ||||
| 3 | GLY | ARG | TRP | PRO | GLY | SER | SER | LEU | TYR | TYR | ||||
| 4 | GLU | VAL | GLU | ILE | LEU | SER | HIS | ASP | SER | THR | ||||
| 5 | SER | GLN | LEU | TYR | THR | VAL | LYS | TYR | LYS | ASP | ||||
| 6 | GLY | THR | GLU | LEU | GLU | LEU | LYS | GLU | ASN | ASP | ||||
| 7 | ILE | LYS | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: Tudor domain, [U-13C; U-15N], 1.17 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9736, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92751 BAD96554 BAG10816 BAG35190 BAG64605 |
| EMBL | CAH92286 |
| GB | AAA59494 AAA59495 AAH20079 ABM81975 ABM85153 |
| REF | NP_001127533 NP_001248262 NP_002287 NP_919424 XP_002760653 |
| SP | Q14739 Q5R7H4 |
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