BMRB Entry 11308
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11308
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the C2H2 zinc finger domain of the protein arginine N-methyltransferase 3 from Mus musculus
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C2H2 zinc finger domain of the protein arginine N-methyltransferase 3 from Mus musculus" . ., .-..
Assembly members:
C2H2 zinc finger domain, polymer, 121 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
C2H2 zinc finger domain: GSSGSSGEPAHGRQHTPCLF
CDRLFASAEETFSHCKLEHQ
FNIDSMVHKHGLEFYGYIKL
INFIRLKNPTVEYMNSIYNP
VPWEKDEYLKPVLEDDLLLQ
FDVEDLYEPVSTPFSSGPSS
G
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 542 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 809 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C2H2 zinc finger domain | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, C2H2 zinc finger domain 121 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | PRO | ALA | ||||
| 2 | HIS | GLY | ARG | GLN | HIS | THR | PRO | CYS | LEU | PHE | ||||
| 3 | CYS | ASP | ARG | LEU | PHE | ALA | SER | ALA | GLU | GLU | ||||
| 4 | THR | PHE | SER | HIS | CYS | LYS | LEU | GLU | HIS | GLN | ||||
| 5 | PHE | ASN | ILE | ASP | SER | MET | VAL | HIS | LYS | HIS | ||||
| 6 | GLY | LEU | GLU | PHE | TYR | GLY | TYR | ILE | LYS | LEU | ||||
| 7 | ILE | ASN | PHE | ILE | ARG | LEU | LYS | ASN | PRO | THR | ||||
| 8 | VAL | GLU | TYR | MET | ASN | SER | ILE | TYR | ASN | PRO | ||||
| 9 | VAL | PRO | TRP | GLU | LYS | ASP | GLU | TYR | LEU | LYS | ||||
| 10 | PRO | VAL | LEU | GLU | ASP | ASP | LEU | LEU | LEU | GLN | ||||
| 11 | PHE | ASP | VAL | GLU | ASP | LEU | TYR | GLU | PRO | VAL | ||||
| 12 | SER | THR | PRO | PHE | SER | SER | GLY | PRO | SER | SER | ||||
| 13 | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: C2H2 zinc finger domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.8996, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAC25300 BAC27531 BAC39708 BAG60456 |
| GB | AAH50775 AAN84530 EAW68334 EDL22978 ERE78768 |
| REF | NP_001138639 NP_598501 XP_003254364 XP_004711656 XP_005578451 |
| SP | Q922H1 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts