BMRB Entry 11310
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11310
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the C-terminal domain of mouse phosphoacetylglucosamine mutase (PAGM)
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Yoneyama, M.; Tochio, N.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, M.; Tochio, N.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C-terminal domain of mouse phosphoacetylglucosamine mutase (PAGM)" . ., .-..
Assembly members:
C-terminal domain, polymer, 112 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
C-terminal domain: GSSGSSGAIYVDLPNRQLKV
KVADRRVISTTDAERQAVTP
PGLQEAINDLVKKYTLARAF
VRPSGTEDIVRVYAEANSQE
SADRLAYEVSLLVFQLAGGI
GERPQPSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 440 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 719 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C-terminal domain | 1 |
Entities:
Entity 1, C-terminal domain 112 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ALA | ILE | TYR | ||||
| 2 | VAL | ASP | LEU | PRO | ASN | ARG | GLN | LEU | LYS | VAL | ||||
| 3 | LYS | VAL | ALA | ASP | ARG | ARG | VAL | ILE | SER | THR | ||||
| 4 | THR | ASP | ALA | GLU | ARG | GLN | ALA | VAL | THR | PRO | ||||
| 5 | PRO | GLY | LEU | GLN | GLU | ALA | ILE | ASN | ASP | LEU | ||||
| 6 | VAL | LYS | LYS | TYR | THR | LEU | ALA | ARG | ALA | PHE | ||||
| 7 | VAL | ARG | PRO | SER | GLY | THR | GLU | ASP | ILE | VAL | ||||
| 8 | ARG | VAL | TYR | ALA | GLU | ALA | ASN | SER | GLN | GLU | ||||
| 9 | SER | ALA | ASP | ARG | LEU | ALA | TYR | GLU | VAL | SER | ||||
| 10 | LEU | LEU | VAL | PHE | GLN | LEU | ALA | GLY | GLY | ILE | ||||
| 11 | GLY | GLU | ARG | PRO | GLN | PRO | SER | GLY | PRO | SER | ||||
| 12 | SER | GLY |
Samples:
sample_1: C-terminal domain of Phosphoacetylglucosamine mutase(PAGM), [U-13C; U-15N], 1 mM; Phosphate Buffer Na 20 mM; NaCl 300 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 320 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delagio, F. - processing
NMRView v5.0.4, Jhonson, B. A. - data analysis
Kujira v0.896, Kobayashi, N. - data analysis
CYANA v1.0.8, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB28834 BAC25733 BAC29478 BAC33692 BAC34728 |
| GB | AAI38701 EDL26495 |
| REF | NP_082628 XP_011240958 |
| SP | Q9CYR6 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts