BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11330

Title: Solution structure of the plus-3 domain of human KIAA0252 protein

Deposition date: 2010-08-10 Original release date: 2011-08-19

Authors: Yoneyama, M.; Kigawa, T.; Tochio, N.; Nameki, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Yoneyama, M.; Kigawa, T.; Tochio, N.; Nameki, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the plus-3 domain of human KIAA0252 protein"  . ., .-..

Assembly members:
Plus-3 domain, Residues 8-143, polymer, 149 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
Plus-3 domain, Residues 8-143: GSSGSSGPPKSQPVSLPEEL NRVRLSRHKLERWCHMPFFA KTVTGCFVRIGIGNHNSKPV YRVAEITGVVETAKVYQLGG TRTNKGLQLRHGNDQRVFRL EFVSNQEFTESEFMKWKEAM FSAGMQLPTLDEINKKELSI KEASGPSSG

Data sets:
Data typeCount
13C chemical shifts631
15N chemical shifts145
1H chemical shifts1006

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Plus-3 domain, Residues 8-1431

Entities:

Entity 1, Plus-3 domain, Residues 8-143 149 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROPROLYS
2   SERGLNPROVALSERLEUPROGLUGLULEU
3   ASNARGVALARGLEUSERARGHISLYSLEU
4   GLUARGTRPCYSHISMETPROPHEPHEALA
5   LYSTHRVALTHRGLYCYSPHEVALARGILE
6   GLYILEGLYASNHISASNSERLYSPROVAL
7   TYRARGVALALAGLUILETHRGLYVALVAL
8   GLUTHRALALYSVALTYRGLNLEUGLYGLY
9   THRARGTHRASNLYSGLYLEUGLNLEUARG
10   HISGLYASNASPGLNARGVALPHEARGLEU
11   GLUPHEVALSERASNGLNGLUPHETHRGLU
12   SERGLUPHEMETLYSTRPLYSGLUALAMET
13   PHESERALAGLYMETGLNLEUPROTHRLEU
14   ASPGLUILEASNLYSLYSGLULEUSERILE
15   LYSGLUALASERGLYPROSERSERGLY

Samples:

sample_1: plus-3 domain, [U-13C; U-15N], 0.73 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9318, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA13382 BAE22595 BAF84991 BAG09677
EMBL CAG31800 CAH91275
GB AAH15052 AAH76973 AAI17849 AAI17850 EAW92490
REF NP_001005068 NP_001102428 NP_001179414 NP_055953 NP_084388
SP A2AQ19 Q5RAD5 Q92541
TPG DAA25394

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts