BMRB Entry 11337
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11337
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Title: Solution structure of the RWD domain of human RWD domain containing protein 3
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Yoneyama, M.; Koshiba, S.; Watabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, M.; Koshiba, S.; Watabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the RWD domain of human RWD domain containing protein 3" . ., .-..
Assembly members:
RWD domain, polymer, 128 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
RWD domain: GSSGSSGMAEPVQEELSVLA
AIFCRPHEWEVLSRSETDGT
VFRIHTKAEGFMDADIPLEL
VFHLPVNYPSCLPGISINSE
QLTRAQCVTVKEKLLEQAES
LLSEPMVHELVLWIQQNLRH
ILSQPETG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 555 |
| 15N chemical shifts | 125 |
| 1H chemical shifts | 897 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RWD domain | 1 |
Entities:
Entity 1, RWD domain 128 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | ALA | GLU | ||||
| 2 | PRO | VAL | GLN | GLU | GLU | LEU | SER | VAL | LEU | ALA | ||||
| 3 | ALA | ILE | PHE | CYS | ARG | PRO | HIS | GLU | TRP | GLU | ||||
| 4 | VAL | LEU | SER | ARG | SER | GLU | THR | ASP | GLY | THR | ||||
| 5 | VAL | PHE | ARG | ILE | HIS | THR | LYS | ALA | GLU | GLY | ||||
| 6 | PHE | MET | ASP | ALA | ASP | ILE | PRO | LEU | GLU | LEU | ||||
| 7 | VAL | PHE | HIS | LEU | PRO | VAL | ASN | TYR | PRO | SER | ||||
| 8 | CYS | LEU | PRO | GLY | ILE | SER | ILE | ASN | SER | GLU | ||||
| 9 | GLN | LEU | THR | ARG | ALA | GLN | CYS | VAL | THR | VAL | ||||
| 10 | LYS | GLU | LYS | LEU | LEU | GLU | GLN | ALA | GLU | SER | ||||
| 11 | LEU | LEU | SER | GLU | PRO | MET | VAL | HIS | GLU | LEU | ||||
| 12 | VAL | LEU | TRP | ILE | GLN | GLN | ASN | LEU | ARG | HIS | ||||
| 13 | ILE | LEU | SER | GLN | PRO | GLU | THR | GLY |
Samples:
sample_1: RWD domain, [U-13C; U-15N], 0.63 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAF85354 BAF85388 BAG59117 BAI46391 |
| EMBL | CAB43254 CAG38524 CAL37651 CAL38135 |
| GB | AAI25143 AIC56138 AIC63357 EAW73019 EAW73020 |
| PIR | T08730 |
| REF | NP_001121614 NP_001186611 NP_001265176 NP_001265177 NP_056300 |
| SP | Q9Y3V2 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts