BMRB Entry 11342
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11342
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Title: Solution Structure of the RING domain of the human RING-box protein 2
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RING domain of the human RING-box protein 2" . ., .-..
Assembly members:
RING domain, polymer, 81 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
RING domain: GSSGSSGMWSWDVECDTCAI
CRVQVMDACLRCQAENKQED
CVVVWGECNHSFHNCCMSLW
VKQNNRCPLCQQDWVVQRIG
K
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 329 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 522 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RING domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
| 4 | ZINC ION no.3 | 2 |
Entities:
Entity 1, RING domain 81 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | TRP | SER | ||||
| 2 | TRP | ASP | VAL | GLU | CYS | ASP | THR | CYS | ALA | ILE | ||||
| 3 | CYS | ARG | VAL | GLN | VAL | MET | ASP | ALA | CYS | LEU | ||||
| 4 | ARG | CYS | GLN | ALA | GLU | ASN | LYS | GLN | GLU | ASP | ||||
| 5 | CYS | VAL | VAL | VAL | TRP | GLY | GLU | CYS | ASN | HIS | ||||
| 6 | SER | PHE | HIS | ASN | CYS | CYS | MET | SER | LEU | TRP | ||||
| 7 | VAL | LYS | GLN | ASN | ASN | ARG | CYS | PRO | LEU | CYS | ||||
| 8 | GLN | GLN | ASP | TRP | VAL | VAL | GLN | ARG | ILE | GLY | ||||
| 9 | LYS |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: RING domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
TOPSPIN v1.3, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE II 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAB22666 BAE26843 BAG34921 BAJ20342 |
| EMBL | CAF94886 CAG32193 CDQ67670 CDQ72674 |
| GB | AAD25961 AAD25962 AAD30147 AAD55984 AAH05966 |
| REF | NP_001012516 NP_001026478 NP_001069188 NP_001100318 NP_001134211 |
| SP | Q9UBF6 Q9WTZ1 |
| TPG | DAA33088 |
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