BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11345

Title: Solution structure of the SWIRM domain of baker's yeast Transcriptional adapter 2

Deposition date: 2010-08-10 Original release date: 2011-08-19

Authors: Yoneyama, M.; Tochio, N.; Koshiba, S.; Tomizawa, T.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Tomizawa, T.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SWIRM domain of baker's yeast Transcriptional adapter 2"  . ., .-..

Assembly members:
SWIRM, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
SWIRM: GSSGSSGNMTISDIQHAPDY ALLSNDEQQLCIQLKILPKP YLVLKEVMFRELLKTGGNLS KSACRELLNIDPIKANRIYD FFQSQNWM

Data sets:
Data typeCount
13C chemical shifts382
15N chemical shifts87
1H chemical shifts627

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SWIRM1

Entities:

Entity 1, SWIRM 88 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNMETTHR
2   ILESERASPILEGLNHISALAPROASPTYR
3   ALALEULEUSERASNASPGLUGLNGLNLEU
4   CYSILEGLNLEULYSILELEUPROLYSPRO
5   TYRLEUVALLEULYSGLUVALMETPHEARG
6   GLULEULEULYSTHRGLYGLYASNLEUSER
7   LYSSERALACYSARGGLULEULEUASNILE
8   ASPPROILELYSALAASNARGILETYRASP
9   PHEPHEGLNSERGLNASNTRPMET

Samples:

sample_1: SWIRM, [U-13C; U-15N], 0.3 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE2 900 MHz

Related Database Links:

PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts