BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11398

Title: Solution structure of PDZ domain in protein XP_110852

Deposition date: 2010-09-09 Original release date: 2011-09-08

Authors: He, F.; Hanaki, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.

Citation: He, F.; Hanaki, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.. "Solution structure of PDZ domain in protein XP_110852"  . ., .-..

Assembly members:
PDZ domain, polymer, 127 residues, Formula weight is not available

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
PDZ domain: GSSGSSGLKGEPDCYALSLE SSEQLTLEIPLNDSGSAGLG VSLKGNKSRETGTDLGIFIK SIIHGGAAFKDGRLRMNDQL IAVNGETLLGKSNHEAMETL RRSMSMEGNIRGMIQLVILR RSGPSSG

Data sets:
Data typeCount
13C chemical shifts501
15N chemical shifts131
1H chemical shifts838

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain1

Entities:

Entity 1, PDZ domain 127 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLEULYSGLY
2   GLUPROASPCYSTYRALALEUSERLEUGLU
3   SERSERGLUGLNLEUTHRLEUGLUILEPRO
4   LEUASNASPSERGLYSERALAGLYLEUGLY
5   VALSERLEULYSGLYASNLYSSERARGGLU
6   THRGLYTHRASPLEUGLYILEPHEILELYS
7   SERILEILEHISGLYGLYALAALAPHELYS
8   ASPGLYARGLEUARGMETASNASPGLNLEU
9   ILEALAVALASNGLYGLUTHRLEULEUGLY
10   LYSSERASNHISGLUALAMETGLUTHRLEU
11   ARGARGSERMETSERMETGLUGLYASNILE
12   ARGGLYMETILEGLNLEUVALILELEUARG
13   ARGSERGLYPROSERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 0.8 mM; phosphate buffer NA 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio F. - processing

NMRView v5.0.4, Johnson B.A. - data analysis

Kujira v0.863, Kobayashi N. - data analysis

CYANA v1.0.8, Guntert P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
GB EDL98917 EGW06143 ERE85499

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts