BMRB Entry 11508

Name: Solution structure of the SH2 domain of Csk in complex with a phosphopeptide from Cbp
Published: 2013-04-08

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PDB ID: 2rsy
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11508
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of the SH2 domain of Csk in complex with a phosphopeptide from Cbp

Deposition date: 2012-09-10 Original release date: 2013-04-08

Authors: Tanaka, Hiroaki; Akagi, Ken-ichi; Oneyama, Chitose; Tanaka, Masakazu; Sasaki, Yuichi; Kanou, Takashi; Lee, Young-Ho; Yokogawa, Daisuke; Debenecker, Marc-Werner; Nakagawa, Atsushi; Okada, Masato; Ikegami, Takahisa

Citation: Tanaka, Hiroaki; Akagi, Ken-ichi; Oneyama, Chitose; Tanaka, Masakazu; Sasaki, Yuichi; Kanou, Takashi; Lee, Young-Ho; Yokogawa, Daisuke; Debenecker, Marc-Werner; Nakagawa, Atsushi; Okada, Masato; Ikegami, Takahisa. "A new SH2 domain binding mode of C-terminal Src kianse to Cbp/PAG"" Not known ., .-..

Assembly members:
Csk_SH2, polymer, 99 residues, 11473.218 Da.
Cbp_phosphopeptide, polymer, 38 residues, 4263.602 Da.

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukayota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
Csk_SH2: GPLGSMPWFHGKITREQAER LLYPPETGLFLVRESTNYPG DYTLCVSCEGKVEHYRIMYH ASKLSIDEEVYFENLMQLVE HYTTDADGLCTRLIKPKVM
Cbp_phosphopeptide: GPLGSKRFSSLSYKSREEDP TLTEEEISAMXSSVNKPG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	581
15N chemical shifts	137
1H chemical shifts	887

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Samples:

sample_1: Csk SH2 domain, [U-13C; U-15N], 0.5 – 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; Cbp phosphopeptide0.5 – 1.0 mM; H2O 90%; D2O, [U-99% 2H], 10%

sample_2: Csk SH2 domain, [U-15N], 0.5 – 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; Cbp phosphopeptide0.5 – 1.0 mM; H2O 90%; D2O, [U-99% 2H], 10%

sample_3: Cbp phosphopeptide, [U-13C; U-15N], 0.5 – 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; Csk SH2 domain0.5 – 1.0 mM; H2O 90%; D2O, [U-99% 2H], 10%

sample_4: Cbp phosphopeptide, [U-15N], 0.5 – 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; Csk SH2 domain0.5 – 1.0 mM; H2O 90%; D2O, [U-99% 2H], 10%

sample_5: Csk SH2 domain, [U-13C; U-15N], 0.5 – 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; Cbp phosphopeptide0.5 – 1.0 mM; D2O, [U-99% 2H], 100%

sample_6: Cbp phosphopeptide, [U-13C; U-15N], 0.5 – 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; Csk SH2 domain0.5 – 1.0 mM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 0.076 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_3	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_3	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_5	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_6	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_5	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_6	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_5	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_6	isotropic	sample_conditions_1
2D DQF-COSY	sample_5	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_3	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_3	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_5	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_6	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_4	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_5	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_6	isotropic	sample_conditions_1
2D CBHD	sample_5	isotropic	sample_conditions_1
2D CBHD	sample_6	isotropic	sample_conditions_1
2D CBHE	sample_5	isotropic	sample_conditions_1
2D CBHE	sample_6	isotropic	sample_conditions_1
3D 13C-filtered/13C-edited NOESY	sample_5	isotropic	sample_conditions_1
3D 13C-filtered/13C-edited NOESY	sample_6	isotropic	sample_conditions_1
3D 13C,15N-filtered/15N-edited NOESY	sample_3	isotropic	sample_conditions_1

UNP	P32577 Q9JM80
PDB	1K9A 2RSY 2RSY
DBJ	BAE23297 BAE33565 BAE42047 BAG60052 BAA95413 BAC30545 BAC40842 BAE33539 BAE42829
EMBL	CAA41484
GB	AAA18766 AAH18394 AAH52006 AAH98863 AAX90624 AAG44565 AAI45329 AAI45762 AAI45764 EDL05179
PRF	1709363A
REF	NP_001025210 NP_001291690 NP_031809 XP_003463668 XP_004628790 NP_001181960 NP_071589 NP_444412 XP_005066844 XP_006530170
SP	P32577 P41241 Q3U1F9 Q9JM80

Biological Magnetic Resonance Data Bank

BMRB Entry 11508

Title: Solution structure of the SH2 domain of Csk in complex with a phosphopeptide from Cbp

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